anti c3b ic3b sections (Vector Laboratories)

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VECTASHIELD Hardset Antifade Mounting Medium with DAPI

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VECTASHIELD Antifade Mounting Medium is a unique stable formula for preserving fluorescence VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes Features Inhibits photobleaching of dyes and fluorescent proteins Ideal refractive index Ready to use No warming necessary Offered with nuclear or cytoskeletal counterstains Available in non hardening or hardening formulations Can be stored without sealing for long term analysis Easy to useVECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping After approximately 15 minutes at room temperature the coverslip will become immobilized and optimal antifade ability and refractive index will be achieved

Catalog Number:

h-1500

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None

Size:

10 ml

Category:

Histology reagents or solutions or stains

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Vector Laboratories anti c3b ic3b sections
VECTASHIELD Hardset Antifade Mounting Medium with DAPI

anti c3b ic3b sections - by Bioz Stars, 2021-02

92/100 stars

Images

1) Product Images from "Monoclonal Antibodies Capable of Inhibiting Complement Downstream of C5 in Multiple Species"

Article Title: Monoclonal Antibodies Capable of Inhibiting Complement Downstream of C5 in Multiple Species

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2020.612402

Testing anti-C7 mAbs in vivo . (A) mAb 73D1 or BB5.1 anti-mouse C5 as positive control was administered IP at a dose of 1 mg/kg to female wildtype mice (n=5 per group); blood was sampled at intervals serum obtained and added to human C7D or C5D serum respectively prior to measuring CP hemolytic activity measured. Controls included C7D and C5D human sera at the same dose, NMS to demonstrate the requirement for human depleted sera and 1% Tween-20 and HBS to set 100% and 0% lysis in the assay. Significance of differences between groups was determined by one-way ANOVA; significant differences and p values are shown in the figure. Error bars are standard errors of triplicates. (B) C7-deficient mice (10 females) were reconstituted with human C7 (500µg; IP), then split into test and control groups (5 in each). After 1 h, test and control animals were injected subcutaneously (SC) with 17E7 mAb or irrelevant isotype control mAb (1 mg) respectively. Blood was collected prior to administration of C7, immediately prior to administration of mAb and 3 h after mAb administration. Hemolytic activity was measured as above. Significance of differences between groups was determined using an unpaired t test; significant differences and p values are shown in the figure. Error bars are standard errors of triplicates. (C) Female Lewis rats were divided into three groups (n = 2 each) and injected intraperitoneally with mAb 2H2 at doses of 10, 20, and 40 mg/kg; blood was collected from all the animals just before mAb administration, 2 h after mAb 2H2 administration, and then every 12 h over 1 week. Sera prepared and hemolytic activity tested in standard CP assays. Significance of differences between groups was determined using an unpaired t test; significant differences and p values are shown in the figure. Error bars are standard errors of triplicates. (D–F) EAMG was induced in rats (5 per group) and either mAb 2H2 or isotype control mAb(10mg/kg) administered at induction. Weight loss (D) and clinical score (E) were measured at intervals; mice were bled at 0, 2, 24, and 48 h, serum harvested and hemolytic activity measured (F) . All animals were killed at 48 h. Results are means of five in each group and vertical bars represent SD. Significance of differences between groups was determined using an unpaired t test except panel E where paired t test was used; significant differences and p values are shown in the figures. (G–K). Soleus muscles were harvested at time of sacrifice (48 h) and snap frozen in OCT. Sections (10 µm) were stained for AChR with TRITC-conjugated a-BuTX; AChR-positive endplates were counted in 10 fields from each animal using ImageJ software. (G) . Sections were stained for C3b/iC3b (H) and C9/MAC (I) and staining quantified as above. Tissue sections from isotype control (J) or 2H2 (K) treated animals were double-stained for AChR together with anti-C3b/iC3b (top panel) or C9/MAC (bottom panel) and imaged on a Zeiss confocal microscope. The scale bar shown is 10 µm; all images were captured at identical magnification. Statistical significance was obtained by unpaired t-test and P

Figure Legend Snippet: Testing anti-C7 mAbs in vivo . (A) mAb 73D1 or BB5.1 anti-mouse C5 as positive control was administered IP at a dose of 1 mg/kg to female wildtype mice (n=5 per group); blood was sampled at intervals serum obtained and added to human C7D or C5D serum respectively prior to measuring CP hemolytic activity measured. Controls included C7D and C5D human sera at the same dose, NMS to demonstrate the requirement for human depleted sera and 1% Tween-20 and HBS to set 100% and 0% lysis in the assay. Significance of differences between groups was determined by one-way ANOVA; significant differences and p values are shown in the figure. Error bars are standard errors of triplicates. (B) C7-deficient mice (10 females) were reconstituted with human C7 (500µg; IP), then split into test and control groups (5 in each). After 1 h, test and control animals were injected subcutaneously (SC) with 17E7 mAb or irrelevant isotype control mAb (1 mg) respectively. Blood was collected prior to administration of C7, immediately prior to administration of mAb and 3 h after mAb administration. Hemolytic activity was measured as above. Significance of differences between groups was determined using an unpaired t test; significant differences and p values are shown in the figure. Error bars are standard errors of triplicates. (C) Female Lewis rats were divided into three groups (n = 2 each) and injected intraperitoneally with mAb 2H2 at doses of 10, 20, and 40 mg/kg; blood was collected from all the animals just before mAb administration, 2 h after mAb 2H2 administration, and then every 12 h over 1 week. Sera prepared and hemolytic activity tested in standard CP assays. Significance of differences between groups was determined using an unpaired t test; significant differences and p values are shown in the figure. Error bars are standard errors of triplicates. (D–F) EAMG was induced in rats (5 per group) and either mAb 2H2 or isotype control mAb(10mg/kg) administered at induction. Weight loss (D) and clinical score (E) were measured at intervals; mice were bled at 0, 2, 24, and 48 h, serum harvested and hemolytic activity measured (F) . All animals were killed at 48 h. Results are means of five in each group and vertical bars represent SD. Significance of differences between groups was determined using an unpaired t test except panel E where paired t test was used; significant differences and p values are shown in the figures. (G–K). Soleus muscles were harvested at time of sacrifice (48 h) and snap frozen in OCT. Sections (10 µm) were stained for AChR with TRITC-conjugated a-BuTX; AChR-positive endplates were counted in 10 fields from each animal using ImageJ software. (G) . Sections were stained for C3b/iC3b (H) and C9/MAC (I) and staining quantified as above. Tissue sections from isotype control (J) or 2H2 (K) treated animals were double-stained for AChR together with anti-C3b/iC3b (top panel) or C9/MAC (bottom panel) and imaged on a Zeiss confocal microscope. The scale bar shown is 10 µm; all images were captured at identical magnification. Statistical significance was obtained by unpaired t-test and P

Techniques Used: In Vivo, Positive Control, Mouse Assay, Activity Assay, Lysis, Injection, Staining, Software, Microscopy

2) Product Images from "Cancer Stem Cells in Head and Neck Cutaneous Squamous Cell Carcinoma Express Cathepsins"

Article Title: Cancer Stem Cells in Head and Neck Cutaneous Squamous Cell Carcinoma Express Cathepsins

Journal: Plastic and Reconstructive Surgery Global Open

doi: 10.1097/GOX.0000000000003042

Representative immunofluorescence sections of moderately differentiated head and neck squamous cell carcinoma tissue samples demonstrating expression of cathepsin B (A, D, red) by the c-MYC + (A, green) cells within the tumor nests (TNs) (A, arrows), as well as cells in the peritumoral stroma (PTS) (A, arrowheads). The cells within the PTS are both OCT4 + (D, green, arrows) and OCT4 − (D, red, arrowheads). Similarly, cathepsin D (B, E, red) was also expressed by the c-MYC + (B, green) cells within the TNs (B, arrows) and the PTS (B, arrowheads), as well as both the OCT4 + (E, green, arrows) and OCT4 − (E, red, arrowheads) cells of the PTS. Cathepsin G (C, F, red) was expressed by some c-MYC + (C, green, arrows) cells within the PTS, but not by the OCT4 + (F, green) cells. Tryptase + (G, green) cells within the PTS also expressed cathepsin G (G, red). All slides were counter-stained with 4′,6′-diamino-2-phenylindole. Original magnification: ×400. Scale bar: 20 µm.

Figure Legend Snippet: Representative immunofluorescence sections of moderately differentiated head and neck squamous cell carcinoma tissue samples demonstrating expression of cathepsin B (A, D, red) by the c-MYC + (A, green) cells within the tumor nests (TNs) (A, arrows), as well as cells in the peritumoral stroma (PTS) (A, arrowheads). The cells within the PTS are both OCT4 + (D, green, arrows) and OCT4 − (D, red, arrowheads). Similarly, cathepsin D (B, E, red) was also expressed by the c-MYC + (B, green) cells within the TNs (B, arrows) and the PTS (B, arrowheads), as well as both the OCT4 + (E, green, arrows) and OCT4 − (E, red, arrowheads) cells of the PTS. Cathepsin G (C, F, red) was expressed by some c-MYC + (C, green, arrows) cells within the PTS, but not by the OCT4 + (F, green) cells. Tryptase + (G, green) cells within the PTS also expressed cathepsin G (G, red). All slides were counter-stained with 4′,6′-diamino-2-phenylindole. Original magnification: ×400. Scale bar: 20 µm.

Techniques Used: Immunofluorescence, Expressing, Staining

Immunohistochemistry:

Article Title: The Identification of Three Cancer Stem Cell Subpopulations within Moderately Differentiated Lip Squamous Cell Carcinoma
Article Snippet: .. All IF IHC-stained slides were mounted in Vectashield HardSet Antifade mounting medium with 4′,6-diamidino-2-phenylindole (cat#H-1500, Vector Laboratories). ..

Laser-Scanning Microscopy:

Article Title: Intraneural convection enhanced delivery of AAVrh20 for targeting primary sensory neurons
Article Snippet: .. All slides were mounted using 4',6-diamidino-2-phenylindole (DAPI) containing mounting media (H-1500, Vector Laboratories, Vector Laboratories, Burlingame, CA, USA) and the fluorescent markers were visualized by laser scanning microscopy (LSM 780, Carl Zeiss, Jena, Germany). .. For the quantification of transduction, native EGFP fluorescence was detected in λ-stack mode and the specific EGFP signal distinguished from a non-specific tissue autofluorescence by linear unmixing as reported in .

Microscopy:

Article Title: Low-Affinity Neurotrophin Receptor p75 Promotes the Transduction of Targeted Lentiviral Vectors to Cholinergic Neurons of Rat Basal Forebrain
Article Snippet: .. After extensive rinses (3 × 15 min in 1 × PBS), samples were air-dried and cover-slipped in Vectashield mounting medium with 4’,6-diamidino-2-phenylindole (H-1500; Vector Laboratories, Burlingame, CA, USA) and image acquisition using a confocal microscope. .. Sulfo-NHS-SS-Biotin (cat. # 21328; Thermo Fisher Scientific, Waltham, MA, USA) was used for lentivirus biotinylation, in line with the manufacturer’s instructions.

Article Title: RIT2, a neuron-specific small guanosine triphosphatase, is expressed in retinal neuronal cells and its promoter is modulated by the POU4 transcription factors
Article Snippet: .. The flat mounts were washed with 0.1% PBST, mounted in VECTASHIELD HardSet Mounting Medium with 4', 6-diamidino-2-phenylindole (H-1500, Vector Laboratories, Burlingame, CA), and examined on an LSM 510 inverted laser scanning confocal microscope (Carl Zeiss, Thornwood, NY). .. For the initial analyses of RIT2 expression by immunohistochemistry of the retinal sections, the mouse eyes were fixed in ice-cold 4% paraformaldehyde in PBS for 1 h, washed in 0.3% PBST, cryoprotected in 20% sucrose in PBS at 4 °C overnight, embedded in OCT Tissue-Tek (Ted Pella, Redding, CA), and cut at 12 μm on a cryostat.

Article Title: Renoprotective Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Immunodeficient Mice Suffering from Acute Kidney Injury
Article Snippet: .. Slides were washed and then mounted with glass coverslips using Vectashield hard set mounting medium with 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) (H-1500; Vector Laboratories) for subsequent observation under the fluorescent microscope. .. Slides were analyzed using a Zeiss Axioplan 2 fluorescence microscope (Carl Zeiss UK Ltd., Hertfordshire, UK) equipped with a triple bandpass filter.

Immunostaining:

Article Title: Human Mutation within Per-Arnt-Sim (PAS) Domain-containing Protein Kinase (PASK) Causes Basal Insulin Hypersecretion *
Article Snippet: .. After permeabilizing sections in 0.1% Triton X-100 overnight at 4 °C, immunostaining was carried out using the following antibodies: mouse monoclonal anti-glucagon antibody (1:200; Sigma), polyclonal anti-swine insulin (1:200; DakoCytomation, Ely, UK), and Alexa 564- or 633-coupled secondary antibodies (1:1000; Molecular Probes), and coverslips were mounted in VECTASHIELD (Vector Laboratories) hardset medium with DAPI. .. Isolated infected islet sections were then imaged using a Leica SP2 upright confocal microscope with an HC PL APO 20 × 0.70 CS objective and the following laser lines: Arg ion (488 nm), HeNe (633 nm), 561 nm diode and UV (350 nm) controlled by Leica LAS AF LiteTM software.

Staining:

Article Title: Role of Macrophage Socs3 in the Pathogenesis of Aortic Dissection
Article Snippet: .. Nuclei were stained with 4',6‐diamidino‐2‐phenylindole in mounting media (#H‐1500; Vector Laboratories, Burlingame, CA). .. Specificity of the fluorescence signal was validated by the comparable level of background signals among the samples with multicolor staining, single color staining, and without staining.

anti c3b ic3b sections (Vector Laboratories)

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Images

1) Product Images from "Monoclonal Antibodies Capable of Inhibiting Complement Downstream of C5 in Multiple Species"

2) Product Images from "Cancer Stem Cells in Head and Neck Cutaneous Squamous Cell Carcinoma Express Cathepsins"

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Immunohistochemistry:

Laser-Scanning Microscopy:

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Immunostaining:

Staining:

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