Structured Review

FUJIFILM dispase
Primary culture procedure. (a) Disinfection and washing of biopsied oral mucosal tissue. (b) Photograph of washed oral mucosal tissue in 35 mm dish. (c) Cutting of oral tissue into 5 mm cubes for <t>dispase</t> treatment. (d) Separation of epithelial layer from connective tissue. (e) Cutting of separated epithelial tissue into 1 mm cubes for trypsin–EDTA treatment. (f) 1000 μL micropipette tip with cutting down the tip at 0-mm long (Normal), 1-mm long, and 2-mm long. (g) Counting suspended cells. Seeding cells into thermo-responsive cell culture inserts at a density of 8 × 10 4 viable cells/cm 2 .
Dispase, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dispase/product/FUJIFILM
Average 99 stars, based on 15 article reviews
Price from $9.99 to $1999.99
dispase - by Bioz Stars, 2021-01
99/100 stars

Images

1) Product Images from "A stable protocol for the fabrication of transplantable human oral mucosal epithelial cell sheets for clinical application"

Article Title: A stable protocol for the fabrication of transplantable human oral mucosal epithelial cell sheets for clinical application

Journal: Regenerative Therapy

doi: 10.1016/j.reth.2019.11.007

Primary culture procedure. (a) Disinfection and washing of biopsied oral mucosal tissue. (b) Photograph of washed oral mucosal tissue in 35 mm dish. (c) Cutting of oral tissue into 5 mm cubes for dispase treatment. (d) Separation of epithelial layer from connective tissue. (e) Cutting of separated epithelial tissue into 1 mm cubes for trypsin–EDTA treatment. (f) 1000 μL micropipette tip with cutting down the tip at 0-mm long (Normal), 1-mm long, and 2-mm long. (g) Counting suspended cells. Seeding cells into thermo-responsive cell culture inserts at a density of 8 × 10 4 viable cells/cm 2 .
Figure Legend Snippet: Primary culture procedure. (a) Disinfection and washing of biopsied oral mucosal tissue. (b) Photograph of washed oral mucosal tissue in 35 mm dish. (c) Cutting of oral tissue into 5 mm cubes for dispase treatment. (d) Separation of epithelial layer from connective tissue. (e) Cutting of separated epithelial tissue into 1 mm cubes for trypsin–EDTA treatment. (f) 1000 μL micropipette tip with cutting down the tip at 0-mm long (Normal), 1-mm long, and 2-mm long. (g) Counting suspended cells. Seeding cells into thermo-responsive cell culture inserts at a density of 8 × 10 4 viable cells/cm 2 .

Techniques Used: Cell Culture

2) Product Images from "Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations"

Article Title: Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations

Journal: Scientific Reports

doi: 10.1038/srep04646

Effects of dissociation without divalent cation and with dispase. (a–c): Comparison between the hiPSCs (253G1) detached and dissociated by PBS −/− (−/−) and those by PBS ca/− (Ca). (ab): Micrograph (a) and FCM analysis (b) of apoptosis marker annexin V-FITC after four hours floating culture (RI: with 5 μM ROCK inhibitor) following detachment and dissociation. The cells were stained by annexin V-FITC (green), propidium iodide (red: late apoptosis or necrosis marker), and Hoechst 33342 (blue: nuclei marker). (c): The number of cells remaining after 3 days culture in ESF9a on fibronectin-coated 10-cm dishes (the initial cell number was 1.2 × 10 5 cells/10-cm dish, mean ± SE, n = 5, t-test). (d–g): Effects of dispase in PBS ca/− for detaching and dissociating cells. (d): Mean cell clump size detached from the plates using PBS ca/− (Ca) and 1 U/ml dispase in PBS ca/− (Ca + Ds). (ef): Micrograph (e) and FCM analysis (f) of annexin V-FITC after four hours floating culture following detachment and dissociation by PBS ca/− or 1 U/ml dispase in PBS ca/− . The staining were the same as (a). (g): Reattachment efficiency. The cells were digested with 0–2 U/ml dispase in PBS ca/− and were plated with ESF9a medium including 5 μM ROCK inhibitor. The numbers of cells were estimated using calcein-AM 1 day after plating and normalized against the PBS ca/− results (0 U/ml dispase). The mean value at 1 U/ml dispase was smaller than 1 ( P
Figure Legend Snippet: Effects of dissociation without divalent cation and with dispase. (a–c): Comparison between the hiPSCs (253G1) detached and dissociated by PBS −/− (−/−) and those by PBS ca/− (Ca). (ab): Micrograph (a) and FCM analysis (b) of apoptosis marker annexin V-FITC after four hours floating culture (RI: with 5 μM ROCK inhibitor) following detachment and dissociation. The cells were stained by annexin V-FITC (green), propidium iodide (red: late apoptosis or necrosis marker), and Hoechst 33342 (blue: nuclei marker). (c): The number of cells remaining after 3 days culture in ESF9a on fibronectin-coated 10-cm dishes (the initial cell number was 1.2 × 10 5 cells/10-cm dish, mean ± SE, n = 5, t-test). (d–g): Effects of dispase in PBS ca/− for detaching and dissociating cells. (d): Mean cell clump size detached from the plates using PBS ca/− (Ca) and 1 U/ml dispase in PBS ca/− (Ca + Ds). (ef): Micrograph (e) and FCM analysis (f) of annexin V-FITC after four hours floating culture following detachment and dissociation by PBS ca/− or 1 U/ml dispase in PBS ca/− . The staining were the same as (a). (g): Reattachment efficiency. The cells were digested with 0–2 U/ml dispase in PBS ca/− and were plated with ESF9a medium including 5 μM ROCK inhibitor. The numbers of cells were estimated using calcein-AM 1 day after plating and normalized against the PBS ca/− results (0 U/ml dispase). The mean value at 1 U/ml dispase was smaller than 1 ( P

Techniques Used: Marker, Staining

3) Product Images from "Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration"

Article Title: Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration

Journal: PLoS ONE

doi: 10.1371/journal.pone.0129096

Isolation of LNGFR + THY-1 + MSCs from SYN. (A) Synovium (SYN) was harvested from patients undergoing total knee arthroplasty surgery and digested with collagenase and dispase. Mesenchymal stem cells (MSCs) were isolated using flow cytometry. DMEM, Dulbecco’s Modified Eagle’s Medium; FBS, fetal bovine serum. (B) Representative flow cytometric profile of SYN-derived cells stained for LNGFR and THY-1. Each population shows in LNGFR + /THY-1 + (red), LNGFR + /THY - (green), LNGFR - /THY + (blue) and LNGFR - /THY - (black) gates. PI, propidium iodide. (C) Number of colonies formed after 2 weeks of culturing 2,000 of the following cells: LNGFR + /THY-1 + , LNGFR + /THY - , LNGFR - /THY + , and LNGFR - /THY - (mean ± standard deviation, n = 4 per group; *p
Figure Legend Snippet: Isolation of LNGFR + THY-1 + MSCs from SYN. (A) Synovium (SYN) was harvested from patients undergoing total knee arthroplasty surgery and digested with collagenase and dispase. Mesenchymal stem cells (MSCs) were isolated using flow cytometry. DMEM, Dulbecco’s Modified Eagle’s Medium; FBS, fetal bovine serum. (B) Representative flow cytometric profile of SYN-derived cells stained for LNGFR and THY-1. Each population shows in LNGFR + /THY-1 + (red), LNGFR + /THY - (green), LNGFR - /THY + (blue) and LNGFR - /THY - (black) gates. PI, propidium iodide. (C) Number of colonies formed after 2 weeks of culturing 2,000 of the following cells: LNGFR + /THY-1 + , LNGFR + /THY - , LNGFR - /THY + , and LNGFR - /THY - (mean ± standard deviation, n = 4 per group; *p

Techniques Used: Isolation, Flow Cytometry, Cytometry, Modification, Derivative Assay, Staining, Standard Deviation

Related Articles

Polymerase Chain Reaction:

Article Title: Low-dose testosterone protects against renal ischemia-reperfusion injury by increasing renal IL-10-to-TNF-α ratio and attenuating T-cell infiltration
Article Snippet: .. PCr was measured using a commercial creatinine kit (Lab Assay, Wako Bio Products, catalog no. 290-65901) by Jaffe's method, according to the manufacturer's protocol, as our laboratory previously described ( ). ..

Concentration Assay:

Article Title: Novel Interplay Between Smad1 and Smad3 Phosphorylation via AGE Regulates the Progression of Diabetic Nephropathy
Article Snippet: .. Creatinine concentration in the urine was measured using LabAssay TM Creatinine (290–65901; Wako). ..

DC Protein Assay:

Article Title: Use of gas chromatography mass spectrometry to elucidate metabolites predicting the phenotypes of IgA nephropathy in hyper IgA mice
Article Snippet: .. The urine total protein levels were measured using a colorimetric assay to determine protein concentration following detergent solubilization (DC protein assay, 500–0116, Bio-Rad Laboratories, Inc. Hercules, CA); the levels were then adjusted according to the urinary creatinine level, which was measured using Jaffe’s reaction (290–65901; WAKO Pure Chemical Industries). ..

Protein Concentration:

Article Title: Use of gas chromatography mass spectrometry to elucidate metabolites predicting the phenotypes of IgA nephropathy in hyper IgA mice
Article Snippet: .. The urine total protein levels were measured using a colorimetric assay to determine protein concentration following detergent solubilization (DC protein assay, 500–0116, Bio-Rad Laboratories, Inc. Hercules, CA); the levels were then adjusted according to the urinary creatinine level, which was measured using Jaffe’s reaction (290–65901; WAKO Pure Chemical Industries). ..

Colorimetric Assay:

Article Title: Use of gas chromatography mass spectrometry to elucidate metabolites predicting the phenotypes of IgA nephropathy in hyper IgA mice
Article Snippet: .. The urine total protein levels were measured using a colorimetric assay to determine protein concentration following detergent solubilization (DC protein assay, 500–0116, Bio-Rad Laboratories, Inc. Hercules, CA); the levels were then adjusted according to the urinary creatinine level, which was measured using Jaffe’s reaction (290–65901; WAKO Pure Chemical Industries). ..

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    FUJIFILM dispase
    Dispase, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dispase/product/FUJIFILM
    Average 99 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2021-01
    99/100 stars
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    92
    FUJIFILM pu dispase ii
    Pu Dispase Ii, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pu dispase ii/product/FUJIFILM
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pu dispase ii - by Bioz Stars, 2021-01
    92/100 stars
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