dispase  (Becton Dickinson)

 
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    Becton Dickinson dispase
    Experimental design. Tooth germs from embryonic day 14.5 mice were obtained and digested with <t>dispase</t> to separate the dental mesenchyme from dental epithelium. Freshly isolated dental mesenchymal cells were designated as P0 and cultured in vitro . RNA samples from the P0, the first (P1), and second (P2) passages were collected before they were submitted for RNA-seq using an Illumina Hiseq™ 2000.
    Dispase, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dispase/product/Becton Dickinson
    Average 92 stars, based on 327 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2021-01
    92/100 stars

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    Images

    1) Product Images from "Insight into the maintenance of odontogenic potential in mouse dental mesenchymal cells based on transcriptomic analysis"

    Article Title: Insight into the maintenance of odontogenic potential in mouse dental mesenchymal cells based on transcriptomic analysis

    Journal: PeerJ

    doi: 10.7717/peerj.1684

    Experimental design. Tooth germs from embryonic day 14.5 mice were obtained and digested with dispase to separate the dental mesenchyme from dental epithelium. Freshly isolated dental mesenchymal cells were designated as P0 and cultured in vitro . RNA samples from the P0, the first (P1), and second (P2) passages were collected before they were submitted for RNA-seq using an Illumina Hiseq™ 2000.
    Figure Legend Snippet: Experimental design. Tooth germs from embryonic day 14.5 mice were obtained and digested with dispase to separate the dental mesenchyme from dental epithelium. Freshly isolated dental mesenchymal cells were designated as P0 and cultured in vitro . RNA samples from the P0, the first (P1), and second (P2) passages were collected before they were submitted for RNA-seq using an Illumina Hiseq™ 2000.

    Techniques Used: Mouse Assay, Isolation, Cell Culture, In Vitro, RNA Sequencing Assay

    2) Product Images from "Adhesive Signature-based, Label-free Isolation of Human Pluripotent Stem Cells"

    Article Title: Adhesive Signature-based, Label-free Isolation of Human Pluripotent Stem Cells

    Journal: Nature methods

    doi: 10.1038/nmeth.2437

    Adhesive differences in spontaneously differentiated pluripotent stem cells enable adhesive force-based enrichment of undifferentiated cells ( a ) μSHEAR-based isolation of UD-hiPSCs (white arrowhead) from SD-hiPSCs (red arrowhead) at 100 dynes cm −2 with high enrichment efficiency irrespective of SD-hiPSC contamination (table). Bottom panel shows non-selective detachment of cells using a trypsin-like enzyme (TrypLE). ( b ) Flow cytometry histograms indicating high purification and survival efficiency of hiPSCs compared to EasySep. ( c ) Flow cytometry scatter plots showing detached UD-hiPSCs (TRA-1-60 + CMPTX + ) and SD-hiPSCs (TRA-1-60 − CMPTX + ) across 10 passages using μSHEAR and TrypLE. ( d ) Enrichment efficiency of hiPSCs when repeatedly passaged by μSHEAR, EDTA, TrypLE, Dispase, or Accutase. hiPSCs from same batch (P 0, 90% TRA-1-60 + ) were used and the recovered culture was propagated for 5–6 days. ( e ) Cell survival on Matrigel after passaging with μSHEAR, manual hand-picking, or TrypLE. ( f ) Growth curves for cells on Matrigel using μSHEAR or hand-picking and starting with an equivalent number of cells at day 0 for each passage (5×10 4 cells). ( g ) Immunostaining for SSEA4 and OCT4 showing μSHEAR-isolated UD-hiPSC cultured on Matrigel retained undifferentiated characteristics across 10 passages. ( h ) Heat-map of expression of stem cell-related and differentiation genes in hiPSCs at P 10 showing no differences in expression between μSHEAR and manual hand-picking, compared to the starting P 0 population. ( i ) Relative expression comparison for stem cell-related genes in isolated hiPSCs at P 10 . Magenta lines indicate two-fold change in gene expression threshold. ( j ) Karyotype analysis of hiPSCs at P 10 . Data report average ± s.d. (* P
    Figure Legend Snippet: Adhesive differences in spontaneously differentiated pluripotent stem cells enable adhesive force-based enrichment of undifferentiated cells ( a ) μSHEAR-based isolation of UD-hiPSCs (white arrowhead) from SD-hiPSCs (red arrowhead) at 100 dynes cm −2 with high enrichment efficiency irrespective of SD-hiPSC contamination (table). Bottom panel shows non-selective detachment of cells using a trypsin-like enzyme (TrypLE). ( b ) Flow cytometry histograms indicating high purification and survival efficiency of hiPSCs compared to EasySep. ( c ) Flow cytometry scatter plots showing detached UD-hiPSCs (TRA-1-60 + CMPTX + ) and SD-hiPSCs (TRA-1-60 − CMPTX + ) across 10 passages using μSHEAR and TrypLE. ( d ) Enrichment efficiency of hiPSCs when repeatedly passaged by μSHEAR, EDTA, TrypLE, Dispase, or Accutase. hiPSCs from same batch (P 0, 90% TRA-1-60 + ) were used and the recovered culture was propagated for 5–6 days. ( e ) Cell survival on Matrigel after passaging with μSHEAR, manual hand-picking, or TrypLE. ( f ) Growth curves for cells on Matrigel using μSHEAR or hand-picking and starting with an equivalent number of cells at day 0 for each passage (5×10 4 cells). ( g ) Immunostaining for SSEA4 and OCT4 showing μSHEAR-isolated UD-hiPSC cultured on Matrigel retained undifferentiated characteristics across 10 passages. ( h ) Heat-map of expression of stem cell-related and differentiation genes in hiPSCs at P 10 showing no differences in expression between μSHEAR and manual hand-picking, compared to the starting P 0 population. ( i ) Relative expression comparison for stem cell-related genes in isolated hiPSCs at P 10 . Magenta lines indicate two-fold change in gene expression threshold. ( j ) Karyotype analysis of hiPSCs at P 10 . Data report average ± s.d. (* P

    Techniques Used: Isolation, Flow Cytometry, Cytometry, Purification, Passaging, Immunostaining, Cell Culture, Expressing

    Related Articles

    Cell Isolation:

    Article Title: c-Maf restrains T-bet-driven programming of CCR6-negative group 3 innate lymphoid cells
    Article Snippet: .. Cell isolation from small intestine and flow cytometry Small intestinal tissue was treated with HBSS buffer (without calcium and magnesium) containing 5 mM EDTA and 10 mM HEPES (pH 7.5) at 37°C for 30 min to remove epithelial cells, minced and digested in HBSS buffer (with calcium and magnesium) containing 10 mM HEPES, 4% FCS, 0.5 mg/ml Collagenase D, 0.5 mg/ml DNaseI (Sigma), 0.5 U/ml Dispase (BD) with constantly stirring at 37°C for 30 min. .. The supernatant was filtered and the remaining tissue was mashed through a 70 μm mesh. siLP cells were separated using a 40%/80% step-gradient (Percoll solution, GE Healthcare).

    Isolation:

    Article Title: Infection of mice with influenza A/WSN/33 (H1N1) virus alters alveolar type II cell phenotype
    Article Snippet: .. The trachea was cannulated, and 2 ml dispase II (5 U/ml in PBS; Becton-Dickinson, San Jose, CA) were injected in the lungs followed by 0.3 ml warmed low-melting-point agarose (1% in PBS) to prevent the isolation of Clara cells and upper airway epithelial cells. ..

    Flow Cytometry:

    Article Title: c-Maf restrains T-bet-driven programming of CCR6-negative group 3 innate lymphoid cells
    Article Snippet: .. Cell isolation from small intestine and flow cytometry Small intestinal tissue was treated with HBSS buffer (without calcium and magnesium) containing 5 mM EDTA and 10 mM HEPES (pH 7.5) at 37°C for 30 min to remove epithelial cells, minced and digested in HBSS buffer (with calcium and magnesium) containing 10 mM HEPES, 4% FCS, 0.5 mg/ml Collagenase D, 0.5 mg/ml DNaseI (Sigma), 0.5 U/ml Dispase (BD) with constantly stirring at 37°C for 30 min. .. The supernatant was filtered and the remaining tissue was mashed through a 70 μm mesh. siLP cells were separated using a 40%/80% step-gradient (Percoll solution, GE Healthcare).

    Blocking Assay:

    Article Title: miR-17 family of microRNAs controls FGF10-mediated embryonic lung epithelial branching morphogenesis through MAPK14 and STAT3 regulation of E-Cadherin distribution
    Article Snippet: .. Lungs from E11.5 embryos were treated with dispase (BD Bioscences) for 5min at 4°C and then transferred to Dulbecco’s Modified Eagle’s Medium:Nutrient Mixture F-12 (DMEM:F12) with 10% fetal bovine serum (FBS) (Invitrogen) to block the enzymatic reaction. .. The epithelial buds were separated from mesenchyme using tungsten needles (Fine Science Tools), embedded in 200µl of Matrigel (BD Biosciences) diluted 1:1 with DMEM:F12 serum-free media containing 250ng/ml FGF10 (R & D Systems), and left to polymerize by incubation at 37°C for 20 minutes.

    Mouse Assay:

    Article Title: Insight into the maintenance of odontogenic potential in mouse dental mesenchymal cells based on transcriptomic analysis
    Article Snippet: .. The lower first molar tooth germs were dissected from the mandibles of E14.5 mice with fine needles and treated with 0.75 mg/ml dispase (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) for 40 min at 37 °C to facilitate separation from dental epithelium. .. The mesenchymal tissues were then incubated in 0.25% trypsin (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 1 min and dissociated with gentle pipetting.

    other:

    Article Title: Febrile-Range Hyperthermia Augments Lipopolysaccharide-Induced Lung Injury by a Mechanism of Enhanced Alveolar Epithelial Apoptosis
    Article Snippet: Collagen IV, Dispase, and biotin-conjugated anti-mouse CD45 and anti-mouse TER-119 Abs were purchased from BD Biosciences (San Jose, CA).

    Modification:

    Article Title: miR-17 family of microRNAs controls FGF10-mediated embryonic lung epithelial branching morphogenesis through MAPK14 and STAT3 regulation of E-Cadherin distribution
    Article Snippet: .. Lungs from E11.5 embryos were treated with dispase (BD Bioscences) for 5min at 4°C and then transferred to Dulbecco’s Modified Eagle’s Medium:Nutrient Mixture F-12 (DMEM:F12) with 10% fetal bovine serum (FBS) (Invitrogen) to block the enzymatic reaction. .. The epithelial buds were separated from mesenchyme using tungsten needles (Fine Science Tools), embedded in 200µl of Matrigel (BD Biosciences) diluted 1:1 with DMEM:F12 serum-free media containing 250ng/ml FGF10 (R & D Systems), and left to polymerize by incubation at 37°C for 20 minutes.

    Injection:

    Article Title: Infection of mice with influenza A/WSN/33 (H1N1) virus alters alveolar type II cell phenotype
    Article Snippet: .. The trachea was cannulated, and 2 ml dispase II (5 U/ml in PBS; Becton-Dickinson, San Jose, CA) were injected in the lungs followed by 0.3 ml warmed low-melting-point agarose (1% in PBS) to prevent the isolation of Clara cells and upper airway epithelial cells. ..

    Similar Products

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    • dispase  (Becton Dickinson)
    92
    Becton Dickinson dispase
    Experimental design. Tooth germs from embryonic day 14.5 mice were obtained and digested with <t>dispase</t> to separate the dental mesenchyme from dental epithelium. Freshly isolated dental mesenchymal cells were designated as P0 and cultured in vitro . RNA samples from the P0, the first (P1), and second (P2) passages were collected before they were submitted for RNA-seq using an Illumina Hiseq™ 2000.
    Dispase, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dispase/product/Becton Dickinson
    Average 92 stars, based on 358 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2021-01
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Experimental design. Tooth germs from embryonic day 14.5 mice were obtained and digested with dispase to separate the dental mesenchyme from dental epithelium. Freshly isolated dental mesenchymal cells were designated as P0 and cultured in vitro . RNA samples from the P0, the first (P1), and second (P2) passages were collected before they were submitted for RNA-seq using an Illumina Hiseq™ 2000.

    Journal: PeerJ

    Article Title: Insight into the maintenance of odontogenic potential in mouse dental mesenchymal cells based on transcriptomic analysis

    doi: 10.7717/peerj.1684

    Figure Lengend Snippet: Experimental design. Tooth germs from embryonic day 14.5 mice were obtained and digested with dispase to separate the dental mesenchyme from dental epithelium. Freshly isolated dental mesenchymal cells were designated as P0 and cultured in vitro . RNA samples from the P0, the first (P1), and second (P2) passages were collected before they were submitted for RNA-seq using an Illumina Hiseq™ 2000.

    Article Snippet: The lower first molar tooth germs were dissected from the mandibles of E14.5 mice with fine needles and treated with 0.75 mg/ml dispase (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) for 40 min at 37 °C to facilitate separation from dental epithelium.

    Techniques: Mouse Assay, Isolation, Cell Culture, In Vitro, RNA Sequencing Assay

    Adhesive differences in spontaneously differentiated pluripotent stem cells enable adhesive force-based enrichment of undifferentiated cells ( a ) μSHEAR-based isolation of UD-hiPSCs (white arrowhead) from SD-hiPSCs (red arrowhead) at 100 dynes cm −2 with high enrichment efficiency irrespective of SD-hiPSC contamination (table). Bottom panel shows non-selective detachment of cells using a trypsin-like enzyme (TrypLE). ( b ) Flow cytometry histograms indicating high purification and survival efficiency of hiPSCs compared to EasySep. ( c ) Flow cytometry scatter plots showing detached UD-hiPSCs (TRA-1-60 + CMPTX + ) and SD-hiPSCs (TRA-1-60 − CMPTX + ) across 10 passages using μSHEAR and TrypLE. ( d ) Enrichment efficiency of hiPSCs when repeatedly passaged by μSHEAR, EDTA, TrypLE, Dispase, or Accutase. hiPSCs from same batch (P 0, 90% TRA-1-60 + ) were used and the recovered culture was propagated for 5–6 days. ( e ) Cell survival on Matrigel after passaging with μSHEAR, manual hand-picking, or TrypLE. ( f ) Growth curves for cells on Matrigel using μSHEAR or hand-picking and starting with an equivalent number of cells at day 0 for each passage (5×10 4 cells). ( g ) Immunostaining for SSEA4 and OCT4 showing μSHEAR-isolated UD-hiPSC cultured on Matrigel retained undifferentiated characteristics across 10 passages. ( h ) Heat-map of expression of stem cell-related and differentiation genes in hiPSCs at P 10 showing no differences in expression between μSHEAR and manual hand-picking, compared to the starting P 0 population. ( i ) Relative expression comparison for stem cell-related genes in isolated hiPSCs at P 10 . Magenta lines indicate two-fold change in gene expression threshold. ( j ) Karyotype analysis of hiPSCs at P 10 . Data report average ± s.d. (* P

    Journal: Nature methods

    Article Title: Adhesive Signature-based, Label-free Isolation of Human Pluripotent Stem Cells

    doi: 10.1038/nmeth.2437

    Figure Lengend Snippet: Adhesive differences in spontaneously differentiated pluripotent stem cells enable adhesive force-based enrichment of undifferentiated cells ( a ) μSHEAR-based isolation of UD-hiPSCs (white arrowhead) from SD-hiPSCs (red arrowhead) at 100 dynes cm −2 with high enrichment efficiency irrespective of SD-hiPSC contamination (table). Bottom panel shows non-selective detachment of cells using a trypsin-like enzyme (TrypLE). ( b ) Flow cytometry histograms indicating high purification and survival efficiency of hiPSCs compared to EasySep. ( c ) Flow cytometry scatter plots showing detached UD-hiPSCs (TRA-1-60 + CMPTX + ) and SD-hiPSCs (TRA-1-60 − CMPTX + ) across 10 passages using μSHEAR and TrypLE. ( d ) Enrichment efficiency of hiPSCs when repeatedly passaged by μSHEAR, EDTA, TrypLE, Dispase, or Accutase. hiPSCs from same batch (P 0, 90% TRA-1-60 + ) were used and the recovered culture was propagated for 5–6 days. ( e ) Cell survival on Matrigel after passaging with μSHEAR, manual hand-picking, or TrypLE. ( f ) Growth curves for cells on Matrigel using μSHEAR or hand-picking and starting with an equivalent number of cells at day 0 for each passage (5×10 4 cells). ( g ) Immunostaining for SSEA4 and OCT4 showing μSHEAR-isolated UD-hiPSC cultured on Matrigel retained undifferentiated characteristics across 10 passages. ( h ) Heat-map of expression of stem cell-related and differentiation genes in hiPSCs at P 10 showing no differences in expression between μSHEAR and manual hand-picking, compared to the starting P 0 population. ( i ) Relative expression comparison for stem cell-related genes in isolated hiPSCs at P 10 . Magenta lines indicate two-fold change in gene expression threshold. ( j ) Karyotype analysis of hiPSCs at P 10 . Data report average ± s.d. (* P

    Article Snippet: Briefly, hiPSC were enzymatically passaged 1:2 with dispase (1 mg mL−1 ) followed by cell scraping onto BD Matrigel (diluted 1:100) in mTeSR1 Medium (STEMCELL Technologies).

    Techniques: Isolation, Flow Cytometry, Cytometry, Purification, Passaging, Immunostaining, Cell Culture, Expressing

    Effect of typical experimental procedures on HT-FC antigen detection. ( A ) 267 out of 344 antigens showed expression of greater than 1% on the “untreated” cell pool (nominal limit of detection of 1%; dashed line). Digestion with a cocktail of enzymes containing trypsin, collagenase, hyaluronidase, dispase and DNAse affected the largest number of antigens, while fixation and cryopreservation affected fewer antigens. ( B ) Examples of validated antigen loss after digestion with individual enzymes trypsin (CD304), collagenase (CD245), dispase (CD120b) and hyaluronidase (CD42a). ( C ) Collagenase and trypsin were typically the most influential enzymes, but all had some influence. Graph shows the number of antigens altered by ≥2-fold relative to untreated cells (blue), or by ≥5 percent-positive cells (red), or both 2-fold and ≥5 percent-positive cells (green). Data for all enzymes is provided in Table S2 .

    Journal: PLoS ONE

    Article Title: Cell Surface Profiling Using High-Throughput Flow Cytometry: A Platform for Biomarker Discovery and Analysis of Cellular Heterogeneity

    doi: 10.1371/journal.pone.0105602

    Figure Lengend Snippet: Effect of typical experimental procedures on HT-FC antigen detection. ( A ) 267 out of 344 antigens showed expression of greater than 1% on the “untreated” cell pool (nominal limit of detection of 1%; dashed line). Digestion with a cocktail of enzymes containing trypsin, collagenase, hyaluronidase, dispase and DNAse affected the largest number of antigens, while fixation and cryopreservation affected fewer antigens. ( B ) Examples of validated antigen loss after digestion with individual enzymes trypsin (CD304), collagenase (CD245), dispase (CD120b) and hyaluronidase (CD42a). ( C ) Collagenase and trypsin were typically the most influential enzymes, but all had some influence. Graph shows the number of antigens altered by ≥2-fold relative to untreated cells (blue), or by ≥5 percent-positive cells (red), or both 2-fold and ≥5 percent-positive cells (green). Data for all enzymes is provided in Table S2 .

    Article Snippet: Cells were incubated at 37°C for 20 minutes whereupon dispase, (Becton Dickinson, 354235, final concentration 2.5 U/mL) was added followed 5 minutes later by 0.25% trypsin (Gibco, 25200-056, final concentration 0.05%) for a final 5 minutes.

    Techniques: Expressing