Structured Review

Santa Cruz Biotechnology dio2 anti rabbit sc 98716 antibodies
Dio2 Anti Rabbit Sc 98716 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dio2 anti rabbit sc 98716 antibodies/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dio2 anti rabbit sc 98716 antibodies - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Santa Cruz Biotechnology dio2 anti rabbit sc 98716 antibodies
QPCR primer list.
Dio2 Anti Rabbit Sc 98716 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dio2 anti rabbit sc 98716 antibodies/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dio2 anti rabbit sc 98716 antibodies - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "Loss of Brain Angiogenesis Inhibitor-3 (BAI3) G-Protein Coupled Receptor in Mice Regulates Adaptive Thermogenesis by Enhancing Energy Expenditure"

Article Title: Loss of Brain Angiogenesis Inhibitor-3 (BAI3) G-Protein Coupled Receptor in Mice Regulates Adaptive Thermogenesis by Enhancing Energy Expenditure

Journal: Metabolites

doi: 10.3390/metabo13060711

QPCR primer list.
Figure Legend Snippet: QPCR primer list.

Techniques Used:


Structured Review

Santa Cruz Biotechnology antibody anti dio2 sc 98716
Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is <t>Dio2</t> . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.
Antibody Anti Dio2 Sc 98716, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti dio2 sc 98716/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
antibody anti dio2 sc 98716 - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade"

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

Journal: PLoS ONE

doi: 10.1371/journal.pone.0026334

Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.
Figure Legend Snippet: Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.

Techniques Used:

(A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).
Figure Legend Snippet: (A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).

Techniques Used: Expressing

(A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.
Figure Legend Snippet: (A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.

Techniques Used: Western Blot, Molecular Weight

(A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.
Figure Legend Snippet: (A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.

Techniques Used:

Primers used for the real time PCR.
Figure Legend Snippet: Primers used for the real time PCR.

Techniques Used:


Structured Review

Santa Cruz Biotechnology anti dio2 sc 98716
Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is <t>Dio2</t> . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.
Anti Dio2 Sc 98716, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti dio2 sc 98716/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti dio2 sc 98716 - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade"

Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

Journal: PLoS ONE

doi: 10.1371/journal.pone.0026334

Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.
Figure Legend Snippet: Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.

Techniques Used:

(A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).
Figure Legend Snippet: (A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).

Techniques Used: Expressing

(A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.
Figure Legend Snippet: (A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.

Techniques Used: Western Blot, Molecular Weight

(A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.
Figure Legend Snippet: (A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.

Techniques Used:

Primers used for the real time PCR.
Figure Legend Snippet: Primers used for the real time PCR.

Techniques Used:


Structured Review

Santa Cruz Biotechnology dio2 sc 98716
Dio2 Sc 98716, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dio2 sc 98716/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dio2 sc 98716 - by Bioz Stars, 2024-04
93/100 stars

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    Santa Cruz Biotechnology dio2 anti rabbit sc 98716 antibodies
    Dio2 Anti Rabbit Sc 98716 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dio2 anti rabbit sc 98716 antibodies/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dio2 anti rabbit sc 98716 antibodies - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology antibody anti dio2 sc 98716
    Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is <t>Dio2</t> . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.
    Antibody Anti Dio2 Sc 98716, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody anti dio2 sc 98716/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody anti dio2 sc 98716 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology anti dio2 sc 98716
    Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is <t>Dio2</t> . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.
    Anti Dio2 Sc 98716, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti dio2 sc 98716/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti dio2 sc 98716 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology dio2 sc 98716
    Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is <t>Dio2</t> . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.
    Dio2 Sc 98716, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dio2 sc 98716/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dio2 sc 98716 - by Bioz Stars, 2024-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.

    Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

    Techniques:

    (A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: (A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).

    Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

    Techniques: Expressing

    (A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: (A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.

    Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

    Techniques: Western Blot, Molecular Weight

    (A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: (A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.

    Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

    Techniques:

    Primers used for the real time PCR.

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: Primers used for the real time PCR.

    Article Snippet: Immunolabeling was performed by standard protocols for tissue fixation and processing, using as primary antibody anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc) and DAPI (Boehringer Mannheim GmbH, Germany) for nuclear staining.

    Techniques:

    Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: Genes up-regulated of at least 1.6 fold at (A) 3 hours and (B) 6 hours of light exposure. The genes that showed an up-regulation in the three replicas are listed and the only gene that appears in both lists is Dio2 . Genes that showed a down-regulation less than 0.7 at (C) 3 hours and (D) 6 hours of exposure to light. The only gene that showed a down-regulation at both timings was Adra1b . Each circle represents one replica. The numbers in each set represent genes that are identified, predicted genes or transcribed locus. In the list, only the identified ones are mentioned.

    Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

    Techniques:

    (A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: (A) Expression of Dio2 showing an up-regulation due to the time of the day (compared to Dark) and as response to light (Circadian Rhythm and Intense Light). (B) Expression of Dio3 showing a down regulation mainly due to light. Data are reported as mean ± S.E.M (N = 6).

    Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

    Techniques: Expressing

    (A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: (A) Representative immunoblot corresponding to DIO2 and DIO3. Although DIO2 shows non-specific bands, a protein band of estimated molecular weight is clearly present in rats exposed to light and absent in dark. MW: Molecular Weight. *:band corresponding to DIO2. (B) Histograms of densitometric measurements of the western blots (N = 3) All band intensities of each protein were compared separately with that of β-actin. The specificity of both antibodies can be observed.

    Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

    Techniques: Western Blot, Molecular Weight

    (A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: (A) In blue for the nuclei (DAPI) and in green for DIO2. (OS photoreceptor outer segment; IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GC ganglion cells). Scale bar = 10μm. (B) Inner nuclear layer nucleus in blue, do not colocalize with DIO2 in green, in the right side the y–z plane, and in the lower left the x–z plane. Scale bar = 5 µm.

    Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

    Techniques:

    Primers used for the real time PCR.

    Journal: PLoS ONE

    Article Title: A Component of Retinal Light Adaptation Mediated by the Thyroid Hormone Cascade

    doi: 10.1371/journal.pone.0026334

    Figure Lengend Snippet: Primers used for the real time PCR.

    Article Snippet: The homogenate was diluted in a sample buffer (20μg), run on SDS-PAGE and western blotted using the following antibodies: anti-Dio2 sc-98716 (Santa Cruz Biotechnology, Inc), anti-Dio3 ab82041 (Abcam, Cambridge, UK). β-Actin HRP- conjugated A3854 (Sigma-Aldrich) was used as housekeeping control.

    Techniques: