Structured Review

Boehringer Mannheim digoxigenin
OLA plate demonstrating the detection of wild-type genotypes (a) and mutant genotypes (b) from randomly selected patients receiving nucleosides. Some wells contain mixtures of genotypes (e.g., row 2, codon 74; row 4, codons 41, 70, and 215), while others reveal only wild-type (e.g., rows 1 and 7, all codons) or mutant (e.g., rows 2 and 6, codons 41 and 215) genotypes. Positive results were easily determined visually by the development of a colored product. The <t>digoxigenin</t> reporter (wild type) was identified by a specific antibody coupled with horseradish peroxidase, which resulted in a brilliant blue color (a). After washing this microtiter plate, the presence of the fluorescein reporter (mutant) was assessed by the production of a deep magenta color due to the alkaline phosphatase labelling of the antibodies to fluorescein (b). The absence of either genotype was also quite clear, because no color was produced in the wells (a and b). A and B, mutants A and B.
Digoxigenin, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine"

Article Title: Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine

Journal: Journal of Clinical Microbiology

doi:

OLA plate demonstrating the detection of wild-type genotypes (a) and mutant genotypes (b) from randomly selected patients receiving nucleosides. Some wells contain mixtures of genotypes (e.g., row 2, codon 74; row 4, codons 41, 70, and 215), while others reveal only wild-type (e.g., rows 1 and 7, all codons) or mutant (e.g., rows 2 and 6, codons 41 and 215) genotypes. Positive results were easily determined visually by the development of a colored product. The digoxigenin reporter (wild type) was identified by a specific antibody coupled with horseradish peroxidase, which resulted in a brilliant blue color (a). After washing this microtiter plate, the presence of the fluorescein reporter (mutant) was assessed by the production of a deep magenta color due to the alkaline phosphatase labelling of the antibodies to fluorescein (b). The absence of either genotype was also quite clear, because no color was produced in the wells (a and b). A and B, mutants A and B.
Figure Legend Snippet: OLA plate demonstrating the detection of wild-type genotypes (a) and mutant genotypes (b) from randomly selected patients receiving nucleosides. Some wells contain mixtures of genotypes (e.g., row 2, codon 74; row 4, codons 41, 70, and 215), while others reveal only wild-type (e.g., rows 1 and 7, all codons) or mutant (e.g., rows 2 and 6, codons 41 and 215) genotypes. Positive results were easily determined visually by the development of a colored product. The digoxigenin reporter (wild type) was identified by a specific antibody coupled with horseradish peroxidase, which resulted in a brilliant blue color (a). After washing this microtiter plate, the presence of the fluorescein reporter (mutant) was assessed by the production of a deep magenta color due to the alkaline phosphatase labelling of the antibodies to fluorescein (b). The absence of either genotype was also quite clear, because no color was produced in the wells (a and b). A and B, mutants A and B.

Techniques Used: Mutagenesis, Produced

2) Product Images from "Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii"

Article Title: Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.47.4.1343-1346.2003

Localization of aadD2 in B. clausii . Total DNA from B. clausii strains NR (lanes 1), OC (lanes 2), T (lanes 3), SIN (lanes 4), and DSM8716 (lanes 5) was digested with restriction enzyme I -Ceu I and subjected to pulsed-field gel electrophoresis. DNA was transferred to a nylon membrane and hybridized successively with rrs (16S rRNA) (left) and aadD2 (right) probes labeled with digoxigenin. The low-molecular-weight I- Ceu I band is barely visible in lanes 2, 3, 4, and 5 (left).
Figure Legend Snippet: Localization of aadD2 in B. clausii . Total DNA from B. clausii strains NR (lanes 1), OC (lanes 2), T (lanes 3), SIN (lanes 4), and DSM8716 (lanes 5) was digested with restriction enzyme I -Ceu I and subjected to pulsed-field gel electrophoresis. DNA was transferred to a nylon membrane and hybridized successively with rrs (16S rRNA) (left) and aadD2 (right) probes labeled with digoxigenin. The low-molecular-weight I- Ceu I band is barely visible in lanes 2, 3, 4, and 5 (left).

Techniques Used: Pulsed-Field Gel, Electrophoresis, Labeling, Molecular Weight

3) Product Images from "Efficient Class II Major Histocompatibility Complex Presentation of Endogenously Synthesized Hepatitis C Virus Core Protein by Epstein-Barr Virus-Transformed B-Lymphoblastoid Cell Lines to CD4+ T Cells"

Article Title: Efficient Class II Major Histocompatibility Complex Presentation of Endogenously Synthesized Hepatitis C Virus Core Protein by Epstein-Barr Virus-Transformed B-Lymphoblastoid Cell Lines to CD4+ T Cells

Journal: Journal of Virology

doi:

IF analysis of protein expression and localization. Expression of core protein in COS cells (A) and B-LCL980-P1 (B) was probed by a MAb to core protein (H-29). (C and D) Localization of core protein in association with the ER in doubly immunostained B cells as detected by a digoxigenin-labeled human polyclonal antibody to core protein (channel 1, FITC) (C) and a MAb to ER membrane protein (PDI) (channel 2, Texas red) (D).
Figure Legend Snippet: IF analysis of protein expression and localization. Expression of core protein in COS cells (A) and B-LCL980-P1 (B) was probed by a MAb to core protein (H-29). (C and D) Localization of core protein in association with the ER in doubly immunostained B cells as detected by a digoxigenin-labeled human polyclonal antibody to core protein (channel 1, FITC) (C) and a MAb to ER membrane protein (PDI) (channel 2, Texas red) (D).

Techniques Used: Expressing, Labeling

Related Articles

other:

Article Title: Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence
Article Snippet: Labelling was done with digoxigenin (digoxigenin-11-dUTP, Boehringer-Mannheim) or biotin (biotin-16-dUTP, Boehringer-Mannheim).

Article Title: Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid `real-time' polymerase chain reaction
Article Snippet: All digoxigenin detection reagents were purchased from Boehringer-Mannheim.

Article Title: Site-Specific Integration in Mammalian Cells Mediated by a New Hybrid Baculovirus-Adeno-Associated Virus Vector
Article Snippet: The digoxigenin-labeled probe was detected by using mouse anti-digoxigenin antibody, digoxigenin-labeled anti-mouse antibody, and fluorescein isothiocyanate (FITC)-labeled anti-digoxigenin antibody (Boehringer Mannheim).

Labeling:

Article Title: Microbial Ecosystem Analysis in Root Canal Infections Refractory to Endodontic Treatment
Article Snippet: The concentration of the purified DNA was determined by spectrophotometric measurement of the absorbance at 260 nm. .. Whole genomic DNA probes were prepared from each of the 107 test strains by labeling 1–3 μg of DNA with digoxigenin (Boehringer Mannheim, Indianapolis, IN, USA), using a random primer technique ( ). .. Approximately 1500 ng of DNA (5 μL) from the amplified sample was placed in a microcentrifuge tube containing 1 ml of TE buffer prior to boiling.

Article Title: “Microbiologic profile of endodontic infections from HIV− and HIV+ patients using MDA and Checkerboard”
Article Snippet: The concentration of the purified DNA was determined by spectrophotometric measurement of the absorbance at 260 nm. .. Whole genomic DNA probes were prepared from each of the 107 test strains by labeling 1 – 3 μg of DNA with digoxigenin (Boehringer Mannheim, Indianapolis, IN, USA) using a random primer technique ( ). .. Approximately 1500 ng of DNA (5 μl) of the amplified sample were placed in a microcentrifuge tube containing 1 mL of TE buffer prior to boiling.

Article Title: Mapping of Abutilon Mosaic Geminivirus Minichromosomes
Article Snippet: For calibration, sequencing reactions of the interesting genome regions were run in parallel according to the manufacturer's recommendations. .. Primers were 3′ end labeled with digoxigenin in a terminal transferase reaction adding digoxigenin-dATP and detected according to the manufacturer's instruction (digoxigenin oligonucleotide tailing kit; digoxigenin-detection kit, Boehringer, Mannheim, Germany). .. Hybridization in 5× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate) with 1% blocking reagent, 0.1% N -lauroylsarcosine, and 0.02% sodium dodecyl sulfate was carried out at 5 to 10°C below the melting temperature of the labeled primers.

Incubation:

Article Title: Thyroid Hormone Regulates reelin and dab1Expression During Brain Development
Article Snippet: .. Sections were blocked in 10% normal goat serum (2 hr) and incubated overnight with an alkaline phosphatase-conjugated antibody to digoxigenin (Boehringer Mannheim, 1:2000). .. After washing, sections were developed with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (Life Technologies, Gaithersburg, MD), mounted on gelatin-coated slides and coverslipped with Mowiol.

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  • 86
    Boehringer Mannheim digoxigenin labeled probes
    Molecular analysis of TER94 ovarian expression. (A) A radiolabeled probe derived from the TER94 cDNA clone was hybridized to Northern blots containing poly(A+) ovarian RNA. The positions of commercially available RNA markers are indicated at left. (B) A <t>digoxigenin-labeled</t> TER94 probe was hybridized against wild-type ovaries prepared for RNA in situ hybridization. The germaria are oriented such that the anterior end is upper right; the bracketed area in A shows germarial region 1.
    Digoxigenin Labeled Probes, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/digoxigenin labeled probes/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    86
    Boehringer Mannheim fluorescein labeled sheep anti digoxigenin conjugate
    Increased NTR1 mRNA expression during toxin A–induced colitis in rats. Rat colonic loops were exposed to either toxin A or buffer. After 1 h, animals were sacrificed, and colonic tissues were processed for in situ hybridization using <t>383-base-digoxigenin–labeled</t> antisense riboprobe encoding for the NTR1 mRNA. Tissues were examined by confocal microscopy. ( a ) Section from a rat colon exposed only to buffer shows the presence of little hybridization signal in the epithelial layer and in cells of the lamina propria. ( b ) Colon exposed for 1 h to toxin A shows increased signal for the NTR1 mRNA primarily in intestinal epithelial cells ( arrows ), but also in cells of the lamina propria ( arrowheads ). ( c ) Colon from toxin A–exposed loop hybridized with a sense riboprobe encoding for the NTR1 mRNA shows absence of specific signal. Results are representative of three experiments for each experimental condition. Scale bar: 50 μm. NTR1 , NT receptor-1.
    Fluorescein Labeled Sheep Anti Digoxigenin Conjugate, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein labeled sheep anti digoxigenin conjugate/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein labeled sheep anti digoxigenin conjugate - by Bioz Stars, 2021-04
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    86
    Boehringer Mannheim anti digoxigenin anti dig alkaline phosphatase fab
    Reverse transcription–polymerase chain reaction (RT–PCR) Southern blot analysis of the cDNA fragment from high-molecular-weight (HMW)-K, LMW-K and T kininogen (KG) mRNAs from liver (L), peritoneal mast cell (M) and submandibular gland (SMG) (S) RNA preparations. A control sample (C) was prepared in the absence of tissue but in the presence of yeast tRNA. The cDNAs amplified by using H1/H2, L1/L2 and T1/L2 primers were subjected to Southern blot analysis. <t>Digoxigenin</t> <t>(DIG)</t> -labelled single-stranded oligonucleotide was used as a probe to detect the fragments of each cDNA, as described in the text. H-KIN, cDNA synthesized by using H1/H2 primers and probed with 5′-DIG-CGAGAGATAACAGCTGGCTGAGCTA-3′ for detection of the cDNA fragment for HMW-K KG mRNA. L-KIN and T-KIN, cDNAs synthesized by using L1/L2 and T1/L2 primers, respectively, and probed with 5′-DIG-AGTCTGCCCTTGTACTCACATGAGT-3′ for detection of cDNA fragments corresponding to LMW-K, T-I and T-II KG mRNAs.
    Anti Digoxigenin Anti Dig Alkaline Phosphatase Fab, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti digoxigenin anti dig alkaline phosphatase fab/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti digoxigenin anti dig alkaline phosphatase fab - by Bioz Stars, 2021-04
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    86
    Boehringer Mannheim digoxigenin
    OLA plate demonstrating the detection of wild-type genotypes (a) and mutant genotypes (b) from randomly selected patients receiving nucleosides. Some wells contain mixtures of genotypes (e.g., row 2, codon 74; row 4, codons 41, 70, and 215), while others reveal only wild-type (e.g., rows 1 and 7, all codons) or mutant (e.g., rows 2 and 6, codons 41 and 215) genotypes. Positive results were easily determined visually by the development of a colored product. The <t>digoxigenin</t> reporter (wild type) was identified by a specific antibody coupled with horseradish peroxidase, which resulted in a brilliant blue color (a). After washing this microtiter plate, the presence of the fluorescein reporter (mutant) was assessed by the production of a deep magenta color due to the alkaline phosphatase labelling of the antibodies to fluorescein (b). The absence of either genotype was also quite clear, because no color was produced in the wells (a and b). A and B, mutants A and B.
    Digoxigenin, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/digoxigenin/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    digoxigenin - by Bioz Stars, 2021-04
    86/100 stars
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    Image Search Results


    Molecular analysis of TER94 ovarian expression. (A) A radiolabeled probe derived from the TER94 cDNA clone was hybridized to Northern blots containing poly(A+) ovarian RNA. The positions of commercially available RNA markers are indicated at left. (B) A digoxigenin-labeled TER94 probe was hybridized against wild-type ovaries prepared for RNA in situ hybridization. The germaria are oriented such that the anterior end is upper right; the bracketed area in A shows germarial region 1.

    Journal: Molecular Biology of the Cell

    Article Title: Identification of TER94, an AAA ATPase Protein, as a Bam-dependent Component of the Drosophila Fusome

    doi:

    Figure Lengend Snippet: Molecular analysis of TER94 ovarian expression. (A) A radiolabeled probe derived from the TER94 cDNA clone was hybridized to Northern blots containing poly(A+) ovarian RNA. The positions of commercially available RNA markers are indicated at left. (B) A digoxigenin-labeled TER94 probe was hybridized against wild-type ovaries prepared for RNA in situ hybridization. The germaria are oriented such that the anterior end is upper right; the bracketed area in A shows germarial region 1.

    Article Snippet: Digoxigenin-labeled probes were prepared from cDNA clones following manufacturer’s instructions (Boehringer Mannheim).

    Techniques: Expressing, Derivative Assay, Northern Blot, Labeling, RNA In Situ Hybridization

    Increased NTR1 mRNA expression during toxin A–induced colitis in rats. Rat colonic loops were exposed to either toxin A or buffer. After 1 h, animals were sacrificed, and colonic tissues were processed for in situ hybridization using 383-base-digoxigenin–labeled antisense riboprobe encoding for the NTR1 mRNA. Tissues were examined by confocal microscopy. ( a ) Section from a rat colon exposed only to buffer shows the presence of little hybridization signal in the epithelial layer and in cells of the lamina propria. ( b ) Colon exposed for 1 h to toxin A shows increased signal for the NTR1 mRNA primarily in intestinal epithelial cells ( arrows ), but also in cells of the lamina propria ( arrowheads ). ( c ) Colon from toxin A–exposed loop hybridized with a sense riboprobe encoding for the NTR1 mRNA shows absence of specific signal. Results are representative of three experiments for each experimental condition. Scale bar: 50 μm. NTR1 , NT receptor-1.

    Journal: Journal of Clinical Investigation

    Article Title: Neurotensin is a proinflammatory neuropeptide in colonic inflammation

    doi:

    Figure Lengend Snippet: Increased NTR1 mRNA expression during toxin A–induced colitis in rats. Rat colonic loops were exposed to either toxin A or buffer. After 1 h, animals were sacrificed, and colonic tissues were processed for in situ hybridization using 383-base-digoxigenin–labeled antisense riboprobe encoding for the NTR1 mRNA. Tissues were examined by confocal microscopy. ( a ) Section from a rat colon exposed only to buffer shows the presence of little hybridization signal in the epithelial layer and in cells of the lamina propria. ( b ) Colon exposed for 1 h to toxin A shows increased signal for the NTR1 mRNA primarily in intestinal epithelial cells ( arrows ), but also in cells of the lamina propria ( arrowheads ). ( c ) Colon from toxin A–exposed loop hybridized with a sense riboprobe encoding for the NTR1 mRNA shows absence of specific signal. Results are representative of three experiments for each experimental condition. Scale bar: 50 μm. NTR1 , NT receptor-1.

    Article Snippet: The localization of NTR1 mRNA was observed after incubation of the sections with a fluorescein-labeled sheep anti-digoxigenin conjugate (Boehringer Mannheim) at a dilution of 1:6 in blocking serum (1% donkey serum, 2% BSA, 0.05 M NH4 Cl, and 0.1% Tween-20.).

    Techniques: Expressing, In Situ Hybridization, Labeling, Confocal Microscopy, Hybridization

    Reverse transcription–polymerase chain reaction (RT–PCR) Southern blot analysis of the cDNA fragment from high-molecular-weight (HMW)-K, LMW-K and T kininogen (KG) mRNAs from liver (L), peritoneal mast cell (M) and submandibular gland (SMG) (S) RNA preparations. A control sample (C) was prepared in the absence of tissue but in the presence of yeast tRNA. The cDNAs amplified by using H1/H2, L1/L2 and T1/L2 primers were subjected to Southern blot analysis. Digoxigenin (DIG) -labelled single-stranded oligonucleotide was used as a probe to detect the fragments of each cDNA, as described in the text. H-KIN, cDNA synthesized by using H1/H2 primers and probed with 5′-DIG-CGAGAGATAACAGCTGGCTGAGCTA-3′ for detection of the cDNA fragment for HMW-K KG mRNA. L-KIN and T-KIN, cDNAs synthesized by using L1/L2 and T1/L2 primers, respectively, and probed with 5′-DIG-AGTCTGCCCTTGTACTCACATGAGT-3′ for detection of cDNA fragments corresponding to LMW-K, T-I and T-II KG mRNAs.

    Journal: Immunology

    Article Title: Expression of kininogens in the connective tissue-type mast cells of the rat

    doi: 10.1046/j.1365-2567.2000.00132.x

    Figure Lengend Snippet: Reverse transcription–polymerase chain reaction (RT–PCR) Southern blot analysis of the cDNA fragment from high-molecular-weight (HMW)-K, LMW-K and T kininogen (KG) mRNAs from liver (L), peritoneal mast cell (M) and submandibular gland (SMG) (S) RNA preparations. A control sample (C) was prepared in the absence of tissue but in the presence of yeast tRNA. The cDNAs amplified by using H1/H2, L1/L2 and T1/L2 primers were subjected to Southern blot analysis. Digoxigenin (DIG) -labelled single-stranded oligonucleotide was used as a probe to detect the fragments of each cDNA, as described in the text. H-KIN, cDNA synthesized by using H1/H2 primers and probed with 5′-DIG-CGAGAGATAACAGCTGGCTGAGCTA-3′ for detection of the cDNA fragment for HMW-K KG mRNA. L-KIN and T-KIN, cDNAs synthesized by using L1/L2 and T1/L2 primers, respectively, and probed with 5′-DIG-AGTCTGCCCTTGTACTCACATGAGT-3′ for detection of cDNA fragments corresponding to LMW-K, T-I and T-II KG mRNAs.

    Article Snippet: Anti-digoxigenin (anti-DIG) alkaline phosphatase Fab, disodium 3-(4-methoxyspiro{1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.1 , ]decan}-4-yl) phenyl phosphate (CSPD) (DIG Luminescent Detection Kit for Nucleic Acids) and yeast tRNA were supplied from Boehringer Mannheim GmbH (Mannheim, Germany).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Southern Blot, Molecular Weight, Amplification, Synthesized

    OLA plate demonstrating the detection of wild-type genotypes (a) and mutant genotypes (b) from randomly selected patients receiving nucleosides. Some wells contain mixtures of genotypes (e.g., row 2, codon 74; row 4, codons 41, 70, and 215), while others reveal only wild-type (e.g., rows 1 and 7, all codons) or mutant (e.g., rows 2 and 6, codons 41 and 215) genotypes. Positive results were easily determined visually by the development of a colored product. The digoxigenin reporter (wild type) was identified by a specific antibody coupled with horseradish peroxidase, which resulted in a brilliant blue color (a). After washing this microtiter plate, the presence of the fluorescein reporter (mutant) was assessed by the production of a deep magenta color due to the alkaline phosphatase labelling of the antibodies to fluorescein (b). The absence of either genotype was also quite clear, because no color was produced in the wells (a and b). A and B, mutants A and B.

    Journal: Journal of Clinical Microbiology

    Article Title: Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine

    doi:

    Figure Lengend Snippet: OLA plate demonstrating the detection of wild-type genotypes (a) and mutant genotypes (b) from randomly selected patients receiving nucleosides. Some wells contain mixtures of genotypes (e.g., row 2, codon 74; row 4, codons 41, 70, and 215), while others reveal only wild-type (e.g., rows 1 and 7, all codons) or mutant (e.g., rows 2 and 6, codons 41 and 215) genotypes. Positive results were easily determined visually by the development of a colored product. The digoxigenin reporter (wild type) was identified by a specific antibody coupled with horseradish peroxidase, which resulted in a brilliant blue color (a). After washing this microtiter plate, the presence of the fluorescein reporter (mutant) was assessed by the production of a deep magenta color due to the alkaline phosphatase labelling of the antibodies to fluorescein (b). The absence of either genotype was also quite clear, because no color was produced in the wells (a and b). A and B, mutants A and B.

    Article Snippet: Ligation primers specific for wild-type or mutant codon sequences (Table ) were modified with a 5′-aminohexylphosphate linker (Aminolink2; Applied Biosystems), and following deprotection, digoxigenin or fluorescein was added to the 5′ end by using N -hydroxysuccinimide esters for these primers (Boehringer Mannheim, Indianapolis, Ind.) ( ).

    Techniques: Mutagenesis, Produced