Digoxigenin, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine"
Article Title: Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine
Journal: Journal of Clinical Microbiology
Figure Legend Snippet: OLA plate demonstrating the detection of wild-type genotypes (a) and mutant genotypes (b) from randomly selected patients receiving nucleosides. Some wells contain mixtures of genotypes (e.g., row 2, codon 74; row 4, codons 41, 70, and 215), while others reveal only wild-type (e.g., rows 1 and 7, all codons) or mutant (e.g., rows 2 and 6, codons 41 and 215) genotypes. Positive results were easily determined visually by the development of a colored product. The digoxigenin reporter (wild type) was identified by a specific antibody coupled with horseradish peroxidase, which resulted in a brilliant blue color (a). After washing this microtiter plate, the presence of the fluorescein reporter (mutant) was assessed by the production of a deep magenta color due to the alkaline phosphatase labelling of the antibodies to fluorescein (b). The absence of either genotype was also quite clear, because no color was produced in the wells (a and b). A and B, mutants A and B.
Techniques Used: Mutagenesis, Produced
2) Product Images from "Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii"
Article Title: Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii
Journal: Antimicrobial Agents and Chemotherapy
Figure Legend Snippet: Localization of aadD2 in B. clausii . Total DNA from B. clausii strains NR (lanes 1), OC (lanes 2), T (lanes 3), SIN (lanes 4), and DSM8716 (lanes 5) was digested with restriction enzyme I -Ceu I and subjected to pulsed-field gel electrophoresis. DNA was transferred to a nylon membrane and hybridized successively with rrs (16S rRNA) (left) and aadD2 (right) probes labeled with digoxigenin. The low-molecular-weight I- Ceu I band is barely visible in lanes 2, 3, 4, and 5 (left).
Techniques Used: Pulsed-Field Gel, Electrophoresis, Labeling, Molecular Weight
3) Product Images from "Efficient Class II Major Histocompatibility Complex Presentation of Endogenously Synthesized Hepatitis C Virus Core Protein by Epstein-Barr Virus-Transformed B-Lymphoblastoid Cell Lines to CD4+ T Cells"
Article Title: Efficient Class II Major Histocompatibility Complex Presentation of Endogenously Synthesized Hepatitis C Virus Core Protein by Epstein-Barr Virus-Transformed B-Lymphoblastoid Cell Lines to CD4+ T Cells
Journal: Journal of Virology
Figure Legend Snippet: IF analysis of protein expression and localization. Expression of core protein in COS cells (A) and B-LCL980-P1 (B) was probed by a MAb to core protein (H-29). (C and D) Localization of core protein in association with the ER in doubly immunostained B cells as detected by a digoxigenin-labeled human polyclonal antibody to core protein (channel 1, FITC) (C) and a MAb to ER membrane protein (PDI) (channel 2, Texas red) (D).
Techniques Used: Expressing, Labeling
Article Title: Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence
Article Snippet: Labelling was done with
Article Title: Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid `real-time' polymerase chain reaction
Article Snippet: All
Article Title: Site-Specific Integration in Mammalian Cells Mediated by a New Hybrid Baculovirus-Adeno-Associated Virus Vector
Article Snippet: The digoxigenin-labeled probe was detected by using mouse anti-digoxigenin antibody, digoxigenin-labeled anti-mouse antibody, and
Article Title: Microbial Ecosystem Analysis in Root Canal Infections Refractory to Endodontic Treatment
Article Snippet: The concentration of the purified DNA was determined by spectrophotometric measurement of the absorbance at 260 nm. .. Whole genomic DNA probes were prepared from each of the 107 test strains by labeling 1–3 μg of DNA with
Article Title: “Microbiologic profile of endodontic infections from HIV− and HIV+ patients using MDA and Checkerboard”
Article Snippet: The concentration of the purified DNA was determined by spectrophotometric measurement of the absorbance at 260 nm. .. Whole genomic DNA probes were prepared from each of the 107 test strains by labeling 1 – 3 μg of DNA with
Article Title: Mapping of Abutilon Mosaic Geminivirus Minichromosomes
Article Snippet: For calibration, sequencing reactions of the interesting genome regions were run in parallel according to the manufacturer's recommendations. .. Primers were 3′ end labeled with digoxigenin in a terminal transferase reaction adding digoxigenin-dATP and detected according to the manufacturer's instruction (
Article Title: Thyroid Hormone Regulates reelin and dab1Expression During Brain Development
Article Snippet: .. Sections were blocked in 10% normal goat serum (2 hr) and incubated overnight with an alkaline phosphatase-conjugated antibody to