differentiation culture time points bmscs scaffold constructs  (Millipore)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    Millipore differentiation culture time points bmscs scaffold constructs
    Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + <t>BMSCs–scaffold</t> group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage <t>ECM</t> component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P
    Differentiation Culture Time Points Bmscs Scaffold Constructs, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/differentiation culture time points bmscs scaffold constructs/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    differentiation culture time points bmscs scaffold constructs - by Bioz Stars, 2021-06
    98/100 stars

    Images

    1) Product Images from "Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration"

    Article Title: Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-020-01845-x

    Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + BMSCs–scaffold group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage ECM component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P
    Figure Legend Snippet: Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + BMSCs–scaffold group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage ECM component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P

    Techniques Used: Immunohistochemistry, Staining

    Related Articles

    Construct:

    Article Title: Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration
    Article Snippet: Cell growth of BMSCs–scaffold construct was observed by inverted phase-contrast microscopy. .. Assessment of ECM accumulation at different differentiation culture time points BMSCs–scaffold constructs cultured for 7, 14, 21, and 28 days were digested in 125 μg/mL papain solution (Sigma) at 55 °C overnight and then centrifuged at 10,000g for 10 min; the supernatant was collected for glycosaminoglycan (GAG) and DNA determination. .. GAG quantification was performed using the Blyscan Glycosaminoglycan Assay (Biocolor, UK); briefly, specimens were complexed with Blyscan dye, the absorbance was measured at 656 nm, and the concentration was calculated using a standard curve.

    Cell Culture:

    Article Title: Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration
    Article Snippet: Cell growth of BMSCs–scaffold construct was observed by inverted phase-contrast microscopy. .. Assessment of ECM accumulation at different differentiation culture time points BMSCs–scaffold constructs cultured for 7, 14, 21, and 28 days were digested in 125 μg/mL papain solution (Sigma) at 55 °C overnight and then centrifuged at 10,000g for 10 min; the supernatant was collected for glycosaminoglycan (GAG) and DNA determination. .. GAG quantification was performed using the Blyscan Glycosaminoglycan Assay (Biocolor, UK); briefly, specimens were complexed with Blyscan dye, the absorbance was measured at 656 nm, and the concentration was calculated using a standard curve.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Millipore differentiation culture time points bmscs scaffold constructs
    Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + <t>BMSCs–scaffold</t> group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage <t>ECM</t> component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P
    Differentiation Culture Time Points Bmscs Scaffold Constructs, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/differentiation culture time points bmscs scaffold constructs/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    differentiation culture time points bmscs scaffold constructs - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

    Image Search Results


    Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + BMSCs–scaffold group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage ECM component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P

    Journal: Stem Cell Research & Therapy

    Article Title: Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

    doi: 10.1186/s13287-020-01845-x

    Figure Lengend Snippet: Histological and immunohistochemical observation and evaluation of the knee joint cartilage. a H E staining of the femoral condyle and tibial plateau cartilage (scale bar = 250 μm). b The Mankin score of histology of femoral condyle and tibial plateau cartilage. The PTH + BMSCs–scaffold group showed lower cartilage degeneration than the BMSCs–scaffold and the Meniscectomy groups. c SO/FG staining and immunohistochemical staining of tibial plateau for cartilage ECM component GAG and Col2 and matrix degradation markers Adamts5 and MMP13 (scale bar = 100 μm). d Semiquantitative analysis of SO/FG staining and immunohistochemical staining. Values for integrated optical density per area (IOD/area) of GAG and Col2 in the PTH + BMSCs–scaffold group were higher and those for Col10 and MMP13 were significantly lower than that in the BMSCs–scaffold group (* P

    Article Snippet: Assessment of ECM accumulation at different differentiation culture time points BMSCs–scaffold constructs cultured for 7, 14, 21, and 28 days were digested in 125 μg/mL papain solution (Sigma) at 55 °C overnight and then centrifuged at 10,000g for 10 min; the supernatant was collected for glycosaminoglycan (GAG) and DNA determination.

    Techniques: Immunohistochemistry, Staining