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cluster of differentiation 68 cd68  (Bio-Rad)


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    Bio-Rad cluster of differentiation 68 cd68
    a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, <t>Cd68),</t> are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, <t>CD68</t> + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.
    Cluster Of Differentiation 68 Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/differentiation+68/Rat+anti+Mouse+CD68/pmc12618493-25-0-10
    Average 96 stars, based on 3269 article reviews
    cluster of differentiation 68 cd68 - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during aging"

    Article Title: Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during aging

    Journal: Nature Communications

    doi: 10.1038/s41467-025-65297-8

    a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, Cd68), are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, CD68 + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.
    Figure Legend Snippet: a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, Cd68), are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, CD68 + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.

    Techniques Used: Marker, Derivative Assay, Expressing, Immunofluorescence

    Related Articles

    Immunohistochemistry:

    Article Title: Age-Related Differences in Lipopolysaccharide-Induced Delirium-like Behavior Implicate the Distinct Microglial Composition in the Hippocampus.
    Article Snippet: .. The following antibodies were used for immunohistochemistry: rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1, for microglia; 1:1000; 019-19741; Wako, Osaka, Japan), goat anti-Iba1 (for microglia; 1:600; 011-27991; Wako), rat anti-cluster of differentiation 68 (CD68, for activated microglia; 1:1500; MCA1957; Bio-Rad, Oxford, UK), rabbit anti-CDKN2A/p16INK4a (p16INK4a, for senescent cells; 1:500; ab211542; Abcam, Waltham, MA, USA), mouse anti-NeuN (for neurons; 1:250; MAB377; Millipore, Burlington, MA, USA), and rabbit anti-c-Fos (for activated neurons; 1:200; cat#2250; Cell Signaling Technology, Danvers, MA, USA). .. Alexa Fluor 488-conjugated donkey anti-goat IgG (1:1000, ab150129, Abcam), Alexa Fluor 594-conjugated donkey antirat IgG (1:1000, ab150156, Abcam), Alexa Fluor 568-conjugated donkey anti-mouse IgG (1:1000, A-10037, Invitrogen, Waltham, MA, USA), and Alexa Fluor 647-conjugated donkey anti-rabbit IgG (1:1000, A-31573, Thermo Fisher Scientific, Waltham, MA, USA) were used as secondary antibodies.

    Article Title: Age-Related Differences in Lipopolysaccharide-Induced Delirium-like Behavior Implicate the Distinct Microglial Composition in the Hippocampus
    Article Snippet: .. The following antibodies were used for immunohistochemistry: rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1, for microglia; 1:1000; 019-19741; Wako, Osaka, Japan), goat anti-Iba1 (for microglia; 1:600; 011-27991; Wako), rat anti-cluster of differentiation 68 (CD68, for activated microglia; 1:1500; MCA1957; Bio-Rad, Oxford, UK), rabbit anti-CDKN2A/p16INK4a (p16 INK4a , for senescent cells; 1:500; ab211542; Abcam, Waltham, MA, USA), mouse anti-NeuN (for neurons; 1:250; MAB377; Millipore, Burlington, MA, USA), and rabbit anti-c-Fos (for activated neurons; 1:200; cat#2250; Cell Signaling Technology, Danvers, MA, USA). .. Alexa Fluor 488-conjugated donkey anti-goat IgG (1:1000, ab150129, Abcam), Alexa Fluor 594-conjugated donkey anti-rat IgG (1:1000, ab150156, Abcam), Alexa Fluor 568-conjugated donkey anti-mouse IgG (1:1000, A-10037, Invitrogen, Waltham, MA, USA), and Alexa Fluor 647-conjugated donkey anti-rabbit IgG (1:1000, A-31573, Thermo Fisher Scientific, Waltham, MA, USA) were used as secondary antibodies.

    Blocking Assay:

    Article Title: Neuroprotective role of sialic-acid-binding immunoglobulin-like lectin-11 in humanized transgenic mice
    Article Snippet: After washing three times with PBS, the slices were incubated with the corresponding secondary antibodies in blocking solution for 2 h at RT (Alexa-488-conjugated goat-anti-rabbit antibody, 1:500, Invitrogen #A11008 and Cy3-conjugated goat anti-mouse IgG F[ab′]2 antibody, 1:200, Dianova #115–166-072). .. For microglia evaluation, sections at Bregma level −3.18 mm were blocked with blocking solution (PBS containing 10% BSA, 0.25% triton X-100) for 2 h at RT followed by overnight incubation at 4°C with primary antibodies against microglial marker ionized calcium binding adaptor molecule1 (Iba1, 1:500, rabbit-anti, Synaptic Systems #234003) and lysosomal marker Cluster of Differentiation 68 (CD68, 1:500, rat-anti-mouse, BioRad #MCA1957) in incubation solution (PBS containing 5% BSA and 0.05% triton X-100). .. After five times washing with 1× PBS, the slices were incubated with corresponding secondary antibodies in blocking solution for 2 h at RT (Alexa-488-conjugated goat-anti-rabbit antibody, 1:400, Invitrogen #A11008 and Cy3-conjugated goat-anti-rat IgG F[ab′]2 antibody, 1:200, Dianova #112–166-072).

    Incubation:

    Article Title: Neuroprotective role of sialic-acid-binding immunoglobulin-like lectin-11 in humanized transgenic mice
    Article Snippet: After washing three times with PBS, the slices were incubated with the corresponding secondary antibodies in blocking solution for 2 h at RT (Alexa-488-conjugated goat-anti-rabbit antibody, 1:500, Invitrogen #A11008 and Cy3-conjugated goat anti-mouse IgG F[ab′]2 antibody, 1:200, Dianova #115–166-072). .. For microglia evaluation, sections at Bregma level −3.18 mm were blocked with blocking solution (PBS containing 10% BSA, 0.25% triton X-100) for 2 h at RT followed by overnight incubation at 4°C with primary antibodies against microglial marker ionized calcium binding adaptor molecule1 (Iba1, 1:500, rabbit-anti, Synaptic Systems #234003) and lysosomal marker Cluster of Differentiation 68 (CD68, 1:500, rat-anti-mouse, BioRad #MCA1957) in incubation solution (PBS containing 5% BSA and 0.05% triton X-100). .. After five times washing with 1× PBS, the slices were incubated with corresponding secondary antibodies in blocking solution for 2 h at RT (Alexa-488-conjugated goat-anti-rabbit antibody, 1:400, Invitrogen #A11008 and Cy3-conjugated goat-anti-rat IgG F[ab′]2 antibody, 1:200, Dianova #112–166-072).

    Article Title: Adult mice with noise-induced hearing loss exhibited temporal ordering memory deficits accompanied by microglia-associated neuroplastic changes in the medial prefrontal cortex.
    Article Snippet: .. Selected free-floating sections were incubated overnight at 4 ◦C with given combinations of the following primary antibodies: rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1, for microglia; 1:1500, 019–19741, Wako, Osaka, Japan), rat anti-cluster of differentiation 68 (CD68, for activated microglia; 1:1500, MCA1957, Bio-Rad, Oxford, UK), goat anti-Iba1 (1:600, 011–27991, Wako), and rabbit anti-PSD95 (1:1000, ab18258, Abcam, Cambridge, UK). .. Sections were further incubated with the appropriate secondary antibody for 2 h in the dark at room temperature: Alexa-568 goat anti-rabbit (1:1000, ab175471, Abcam), Alexa-488 goat anti-rat (1:1000, A-11006, Thermo Fisher Scientific, Waltham, USA), Alexa488 donkey anti-goat (1:1000, ab150129, Abcam), Alexa-594 donkey anti-rabbit (1:1000, ab150064, Abcam), and Alexa-647 donkey antirabbit (1:1000, A-31573, Thermo Fisher Scientific).

    Marker:

    Article Title: Neuroprotective role of sialic-acid-binding immunoglobulin-like lectin-11 in humanized transgenic mice
    Article Snippet: After washing three times with PBS, the slices were incubated with the corresponding secondary antibodies in blocking solution for 2 h at RT (Alexa-488-conjugated goat-anti-rabbit antibody, 1:500, Invitrogen #A11008 and Cy3-conjugated goat anti-mouse IgG F[ab′]2 antibody, 1:200, Dianova #115–166-072). .. For microglia evaluation, sections at Bregma level −3.18 mm were blocked with blocking solution (PBS containing 10% BSA, 0.25% triton X-100) for 2 h at RT followed by overnight incubation at 4°C with primary antibodies against microglial marker ionized calcium binding adaptor molecule1 (Iba1, 1:500, rabbit-anti, Synaptic Systems #234003) and lysosomal marker Cluster of Differentiation 68 (CD68, 1:500, rat-anti-mouse, BioRad #MCA1957) in incubation solution (PBS containing 5% BSA and 0.05% triton X-100). .. After five times washing with 1× PBS, the slices were incubated with corresponding secondary antibodies in blocking solution for 2 h at RT (Alexa-488-conjugated goat-anti-rabbit antibody, 1:400, Invitrogen #A11008 and Cy3-conjugated goat-anti-rat IgG F[ab′]2 antibody, 1:200, Dianova #112–166-072).

    Binding Assay:

    Article Title: Neuroprotective role of sialic-acid-binding immunoglobulin-like lectin-11 in humanized transgenic mice
    Article Snippet: After washing three times with PBS, the slices were incubated with the corresponding secondary antibodies in blocking solution for 2 h at RT (Alexa-488-conjugated goat-anti-rabbit antibody, 1:500, Invitrogen #A11008 and Cy3-conjugated goat anti-mouse IgG F[ab′]2 antibody, 1:200, Dianova #115–166-072). .. For microglia evaluation, sections at Bregma level −3.18 mm were blocked with blocking solution (PBS containing 10% BSA, 0.25% triton X-100) for 2 h at RT followed by overnight incubation at 4°C with primary antibodies against microglial marker ionized calcium binding adaptor molecule1 (Iba1, 1:500, rabbit-anti, Synaptic Systems #234003) and lysosomal marker Cluster of Differentiation 68 (CD68, 1:500, rat-anti-mouse, BioRad #MCA1957) in incubation solution (PBS containing 5% BSA and 0.05% triton X-100). .. After five times washing with 1× PBS, the slices were incubated with corresponding secondary antibodies in blocking solution for 2 h at RT (Alexa-488-conjugated goat-anti-rabbit antibody, 1:400, Invitrogen #A11008 and Cy3-conjugated goat-anti-rat IgG F[ab′]2 antibody, 1:200, Dianova #112–166-072).

    Staining:

    Article Title: A novel model of light-induced middle cerebral artery occlusion and recanalization in mice
    Article Snippet: We used Skeleton analysis (ImageJ software) to determine the total length of the processes, the number of branches, the number of junctions, the number of end-points of the branches, the junction voxels, the average branch length, and the maximum branch length of the astrocytes for each ROI. .. Microglial cells and phagosomes were stained respectively with ionized calcium-binding adapter molecule 1 (Iba1; 1:500; rabbit anti-Iba1, Wako, Osaka, Japan, 019–19741) and Cluster of Differentiation 68 (CD68; 1:250; rat anti-CD68, BioRad, #MCA1957). .. Images were acquired with a confocal microscope (Carl Zeiss LSM 900) using a 40× objective with a single z-stack.

    Article Title: A novel model of light-induced middle cerebral artery occlusion and recanalization in mice.
    Article Snippet: We used Skeleton analysis (ImageJ software) to determine the total length of the processes, the number of branches, the number of junctions, the number of end-points of the branches, the junction voxels, the average branch length, and the maximum branch length of the astrocytes for each ROI. .. Microglial cells andphagosomeswere stained respectivelywith ionized calcium-binding adapter molecule 1 (Iba1; 1:500; rabbit anti-Iba1, Wako, Osaka, Japan, 019–19741) and Cluster of Differentiation 68 (CD68; 1:250; rat anti-CD68, BioRad, #MCA1957). .. Images were acquired with a confocal microscope (Carl Zeiss LSM900) using a 40× objectivewith a single z-stack.



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    a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, <t>Cd68),</t> are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, <t>CD68</t> + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.
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    Cell Signaling Technology Inc differentiation 68
    a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, <t>Cd68),</t> are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, <t>CD68</t> + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.
    Differentiation 68, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, <t>Cd68),</t> are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, <t>CD68</t> + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.
    Differentiation 68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, Cd68), are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, CD68 + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during aging

    doi: 10.1038/s41467-025-65297-8

    Figure Lengend Snippet: a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, Cd68), are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, CD68 + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.

    Article Snippet: Cluster of differentiation 68 (CD68) 1:250 , Mouse , MCA1957 Biorad Hercules, CA, USA.

    Techniques: Marker, Derivative Assay, Expressing, Immunofluorescence