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Structured Review

Dade Behring diff quick stain kit
Diff Quick Stain Kit, supplied by Dade Behring, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick+kit/diff+quick/pm42162069-128-8-11
Average 86 stars, based on 1 article reviews
diff quick stain kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Membrane:

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer.
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: 14-3-3ζ silencing retards tongue squamous cell carcinoma progression by inhibiting cell survival and migration.
Article Snippet: Cell migration assay Cell migration assays were performed in 24-well Boyden chambers (8-μm pores, BD Biosciences, Franklin Lakes, NJ, USA) as previously described.18 The cells were plated at a density of 1 × 106 cells per 100 μl medium in the inserts, and incubated for 3 h at 37 °C followed by staining using a Diff-Quick kit (Dade Behring, Carpenteria, CA, USA).

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: Targeting tumor-stroma communication by blocking endothelin-1 receptors sensitizes high-grade serous ovarian cancer to PARP inhibition
Article Snippet: After 24 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, USA) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: The endothelin-1-driven tumor-stroma feed-forward loops in high-grade serous ovarian cancer
Article Snippet: After 48 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer
Article Snippet: After 24 h, the invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: Evaluation of lung VEGF-A transduction during hyperoxia-induced injury in rats
Article Snippet: The cells were fixed and stained with Diff-Quick kit (Dade Behring, France).

Article Title: Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells.
Article Snippet: Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones.. CD4þ T cell-mediated immune response is involved in the progression of periodontitis.. Tobacco consumption increases the risk of periodontal disease.

Staining:

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer.
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: 14-3-3ζ silencing retards tongue squamous cell carcinoma progression by inhibiting cell survival and migration.
Article Snippet: Cell migration assay Cell migration assays were performed in 24-well Boyden chambers (8-μm pores, BD Biosciences, Franklin Lakes, NJ, USA) as previously described.18 The cells were plated at a density of 1 × 106 cells per 100 μl medium in the inserts, and incubated for 3 h at 37 °C followed by staining using a Diff-Quick kit (Dade Behring, Carpenteria, CA, USA).

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: Targeting tumor-stroma communication by blocking endothelin-1 receptors sensitizes high-grade serous ovarian cancer to PARP inhibition
Article Snippet: After 24 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, USA) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: The endothelin-1-driven tumor-stroma feed-forward loops in high-grade serous ovarian cancer
Article Snippet: After 48 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer
Article Snippet: After 24 h, the invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: Evaluation of lung VEGF-A transduction during hyperoxia-induced injury in rats
Article Snippet: The cells were fixed and stained with Diff-Quick kit (Dade Behring, France).

Article Title: Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells.
Article Snippet: Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones.. CD4þ T cell-mediated immune response is involved in the progression of periodontitis.. Tobacco consumption increases the risk of periodontal disease.

Diff-Quik:

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer.
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: 14-3-3ζ silencing retards tongue squamous cell carcinoma progression by inhibiting cell survival and migration.
Article Snippet: Cell migration assay Cell migration assays were performed in 24-well Boyden chambers (8-μm pores, BD Biosciences, Franklin Lakes, NJ, USA) as previously described.18 The cells were plated at a density of 1 × 106 cells per 100 μl medium in the inserts, and incubated for 3 h at 37 °C followed by staining using a Diff-Quick kit (Dade Behring, Carpenteria, CA, USA).

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: Targeting tumor-stroma communication by blocking endothelin-1 receptors sensitizes high-grade serous ovarian cancer to PARP inhibition
Article Snippet: After 24 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, USA) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: The endothelin-1-driven tumor-stroma feed-forward loops in high-grade serous ovarian cancer
Article Snippet: After 48 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer
Article Snippet: After 24 h, the invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: Evaluation of lung VEGF-A transduction during hyperoxia-induced injury in rats
Article Snippet: The cells were fixed and stained with Diff-Quick kit (Dade Behring, France).

Article Title: Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells.
Article Snippet: Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones.. CD4þ T cell-mediated immune response is involved in the progression of periodontitis.. Tobacco consumption increases the risk of periodontal disease.

Cell Migration Assay:

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer.
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: 14-3-3ζ silencing retards tongue squamous cell carcinoma progression by inhibiting cell survival and migration.
Article Snippet: Cell migration assay Cell migration assays were performed in 24-well Boyden chambers (8-μm pores, BD Biosciences, Franklin Lakes, NJ, USA) as previously described.18 The cells were plated at a density of 1 × 106 cells per 100 μl medium in the inserts, and incubated for 3 h at 37 °C followed by staining using a Diff-Quick kit (Dade Behring, Carpenteria, CA, USA).

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: Targeting tumor-stroma communication by blocking endothelin-1 receptors sensitizes high-grade serous ovarian cancer to PARP inhibition
Article Snippet: After 24 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, USA) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: The endothelin-1-driven tumor-stroma feed-forward loops in high-grade serous ovarian cancer
Article Snippet: After 48 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer
Article Snippet: After 24 h, the invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: Evaluation of lung VEGF-A transduction during hyperoxia-induced injury in rats
Article Snippet: The cells were fixed and stained with Diff-Quick kit (Dade Behring, France).

Article Title: Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells.
Article Snippet: Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones.. CD4þ T cell-mediated immune response is involved in the progression of periodontitis.. Tobacco consumption increases the risk of periodontal disease.

Migration:

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer.
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: 14-3-3ζ silencing retards tongue squamous cell carcinoma progression by inhibiting cell survival and migration.
Article Snippet: Cell migration assay Cell migration assays were performed in 24-well Boyden chambers (8-μm pores, BD Biosciences, Franklin Lakes, NJ, USA) as previously described.18 The cells were plated at a density of 1 × 106 cells per 100 μl medium in the inserts, and incubated for 3 h at 37 °C followed by staining using a Diff-Quick kit (Dade Behring, Carpenteria, CA, USA).

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: Targeting tumor-stroma communication by blocking endothelin-1 receptors sensitizes high-grade serous ovarian cancer to PARP inhibition
Article Snippet: After 24 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, USA) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: The endothelin-1-driven tumor-stroma feed-forward loops in high-grade serous ovarian cancer
Article Snippet: After 48 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer
Article Snippet: After 24 h, the invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: Evaluation of lung VEGF-A transduction during hyperoxia-induced injury in rats
Article Snippet: The cells were fixed and stained with Diff-Quick kit (Dade Behring, France).

Article Title: Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells.
Article Snippet: Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones.. CD4þ T cell-mediated immune response is involved in the progression of periodontitis.. Tobacco consumption increases the risk of periodontal disease.

Incubation:

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer.
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: 14-3-3ζ silencing retards tongue squamous cell carcinoma progression by inhibiting cell survival and migration.
Article Snippet: Cell migration assay Cell migration assays were performed in 24-well Boyden chambers (8-μm pores, BD Biosciences, Franklin Lakes, NJ, USA) as previously described.18 The cells were plated at a density of 1 × 106 cells per 100 μl medium in the inserts, and incubated for 3 h at 37 °C followed by staining using a Diff-Quick kit (Dade Behring, Carpenteria, CA, USA).

Article Title: β-arrestin1/YAP/mutant p53 complexes orchestrate the endothelin A receptor signaling in high-grade serous ovarian cancer
Article Snippet: Cells on the upper part of the membrane were scraped using a cotton swab and the invading cells were stained using Diff-Quick kit (Dade Behring, Deerfield, Illinois, USA).

Article Title: Targeting tumor-stroma communication by blocking endothelin-1 receptors sensitizes high-grade serous ovarian cancer to PARP inhibition
Article Snippet: After 24 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, USA) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: The endothelin-1-driven tumor-stroma feed-forward loops in high-grade serous ovarian cancer
Article Snippet: After 48 h, the migrating and invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer
Article Snippet: After 24 h, the invading cells were visualized using a Diff-Quick kit (Dade Behring, IL, U.S.A.) and detected under a ZOE Fluorescent Cell Imager (Bio-Rad).

Article Title: Evaluation of lung VEGF-A transduction during hyperoxia-induced injury in rats
Article Snippet: The cells were fixed and stained with Diff-Quick kit (Dade Behring, France).

Article Title: Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells.
Article Snippet: Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones.. CD4þ T cell-mediated immune response is involved in the progression of periodontitis.. Tobacco consumption increases the risk of periodontal disease.



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
Diff Quick Staining Kit, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction