diaminobenzidine  (Vector Laboratories)


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    Name:
    DAB Peroxidase HRP Substrate Kit with Nickel 3 3 diaminobenzidine
    Description:
    DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
    Catalog Number:
    SK-4100
    Price:
    None
    Category:
    Proteins
    Size:
    1 Kit
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    Structured Review

    Vector Laboratories diaminobenzidine
    DAB Peroxidase HRP Substrate Kit with Nickel 3 3 diaminobenzidine
    DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
    https://www.bioz.com/result/diaminobenzidine/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "Lymphoid hyperplasia in transgenic mice over-expressing a secreted form of the human interleukin-1? gene product"

    Article Title: Lymphoid hyperplasia in transgenic mice over-expressing a secreted form of the human interleukin-1? gene product

    Journal: Immunology

    doi: 10.1046/j.1365-2567.1999.00655.x

    Infiltration of T lymphocytes in colon of the transgenic mice. Parallel sections of colon from control (a–c) and transgenic (d–f) mice were stained with B220 mAb (a and d), CD4 mAb (b and e) and CD8 mAb (c and f), respectively. (g) Staining of transgenic colon with an isotype-matched (IgG2a) control antibody. Staining of control lymph node with isotype-matched control antibody were completely negative as well (data not shown). The bound antibodies were made visible by use of diaminobenzidine (DAB). Sections were counterstained with methyl green. The positively stained cells are brown. ((a–g) Scale bar 100 μm.) The results are representative of several sections from two different animals.
    Figure Legend Snippet: Infiltration of T lymphocytes in colon of the transgenic mice. Parallel sections of colon from control (a–c) and transgenic (d–f) mice were stained with B220 mAb (a and d), CD4 mAb (b and e) and CD8 mAb (c and f), respectively. (g) Staining of transgenic colon with an isotype-matched (IgG2a) control antibody. Staining of control lymph node with isotype-matched control antibody were completely negative as well (data not shown). The bound antibodies were made visible by use of diaminobenzidine (DAB). Sections were counterstained with methyl green. The positively stained cells are brown. ((a–g) Scale bar 100 μm.) The results are representative of several sections from two different animals.

    Techniques Used: Transgenic Assay, Mouse Assay, Staining

    Altered localization of CD4 + T cells in lymph node of the transgenic mice. Parallel sections of lymph node from control (a, c) and transgenic (b, d) mice were stained with B220 mAb (a and b) and CD4 mAb (c and d), respectively. (e) Staining of transgenic lymph node with an isotype-matched (IgG2a) control antibody. Staining of control lymph node with isotype-matched control antibody were negative as well (data not shown). The bound antibodies were made visible by use of diaminobenzidine (DAB). Sections were counterstained with methyl green. The positively stained cells are brown. Note that the CD4 + T cells in the transgenic organs are intervening the majority of the lymph node including normally B-cell restricted areas which is not seen in the control (a,b, bar = 50 μm; c–e, bar = 100 μm). The results are representative of several sections from three different animals.
    Figure Legend Snippet: Altered localization of CD4 + T cells in lymph node of the transgenic mice. Parallel sections of lymph node from control (a, c) and transgenic (b, d) mice were stained with B220 mAb (a and b) and CD4 mAb (c and d), respectively. (e) Staining of transgenic lymph node with an isotype-matched (IgG2a) control antibody. Staining of control lymph node with isotype-matched control antibody were negative as well (data not shown). The bound antibodies were made visible by use of diaminobenzidine (DAB). Sections were counterstained with methyl green. The positively stained cells are brown. Note that the CD4 + T cells in the transgenic organs are intervening the majority of the lymph node including normally B-cell restricted areas which is not seen in the control (a,b, bar = 50 μm; c–e, bar = 100 μm). The results are representative of several sections from three different animals.

    Techniques Used: Transgenic Assay, Mouse Assay, Staining

    2) Product Images from "Specific norovirus interaction with Lewis x and Lewis a on human intestinal inflammatory mucosa during refractory inflammatory bowel disease"

    Article Title: Specific norovirus interaction with Lewis x and Lewis a on human intestinal inflammatory mucosa during refractory inflammatory bowel disease

    Journal: bioRxiv

    doi: 10.1101/2020.11.24.397125

    VLP binding specificity demonstrated in a healthy duodenal sample from a blood group A patient who underwent duodenopancreatectomy. Slides were pretreated with either 50mM sodium periodate (NaIO4), α1,2 fucosidase (boiled or cold) before incubation with HuNoV GII.4 VLP. Mutated ΔD373 GII.4 VLPs were used as negative controls. For this experiment and the following, VLP binding was detected with GII.4 VP1-specific MAb labeled with peroxydase. Peroxydase activity was detected by colorimetry using H2O2 and 3,3’-diaminobenzidine (DAB) giving a brown staining. Mock and pretreatments are indicated above each image.
    Figure Legend Snippet: VLP binding specificity demonstrated in a healthy duodenal sample from a blood group A patient who underwent duodenopancreatectomy. Slides were pretreated with either 50mM sodium periodate (NaIO4), α1,2 fucosidase (boiled or cold) before incubation with HuNoV GII.4 VLP. Mutated ΔD373 GII.4 VLPs were used as negative controls. For this experiment and the following, VLP binding was detected with GII.4 VP1-specific MAb labeled with peroxydase. Peroxydase activity was detected by colorimetry using H2O2 and 3,3’-diaminobenzidine (DAB) giving a brown staining. Mock and pretreatments are indicated above each image.

    Techniques Used: Binding Assay, Incubation, Labeling, Activity Assay, Colorimetric Assay, Staining

    3) Product Images from "Cutaneous Structural and Biochemical Correlates of Foot Complications in High-Risk Diabetes"

    Article Title: Cutaneous Structural and Biochemical Correlates of Foot Complications in High-Risk Diabetes

    Journal: Diabetes Care

    doi: 10.2337/dc11-2076

    A : Comparison of percentage of PAR + nuclei in different subject groups. Lower leg skin tissue from nondiabetic and diabetic subjects was maintained in organ culture for 8 days. Paraffin sections (4 μm) were prepared. Mouse monoclonal anti-PAR antibody (1:400) was applied to sections. Immunoreactivity was revealed using diaminobenzidine, and sections were counterstained with hematoxylin. Data are mean ± SD. P
    Figure Legend Snippet: A : Comparison of percentage of PAR + nuclei in different subject groups. Lower leg skin tissue from nondiabetic and diabetic subjects was maintained in organ culture for 8 days. Paraffin sections (4 μm) were prepared. Mouse monoclonal anti-PAR antibody (1:400) was applied to sections. Immunoreactivity was revealed using diaminobenzidine, and sections were counterstained with hematoxylin. Data are mean ± SD. P

    Techniques Used: Organ Culture

    A : Comparison of type 1 procollagen abundance in the lower leg in different subject groups. Skin tissue from nondiabetic and diabetic subjects was maintained in organ culture for 8 days. At the end of the incubation period, sections were dewaxed, blocked with 10% horse serum, and incubated with antibodies to type 1 procollagen, and the immunoreactivity was revealed using a VECTASTAIN Universal Elite ABC Kit and diaminobenzidine. The area and density of staining was determined by image analysis using Scion Image. Data are mean ± SD. P
    Figure Legend Snippet: A : Comparison of type 1 procollagen abundance in the lower leg in different subject groups. Skin tissue from nondiabetic and diabetic subjects was maintained in organ culture for 8 days. At the end of the incubation period, sections were dewaxed, blocked with 10% horse serum, and incubated with antibodies to type 1 procollagen, and the immunoreactivity was revealed using a VECTASTAIN Universal Elite ABC Kit and diaminobenzidine. The area and density of staining was determined by image analysis using Scion Image. Data are mean ± SD. P

    Techniques Used: Organ Culture, Incubation, Staining

    Related Articles

    Incubation:

    Article Title: Inhibition of Smooth Muscle Cell Proliferation and Migration by a Talin Modulator Attenuates Neointimal Formation after Femoral Arterial Injury
    Article Snippet: The sections were then incubated with α-smooth muscle actin (α-SMA; A2547, Sigma Aldrich) antibodies for 1 hour at RT and then with biotinylated anti-mouse IgG antibodies (BA-2000, Vector, Burlingame, CA, USA) for 30 minutes at RT. .. Subsequently, the sections were incubated with the ABC reagent (PK-6100) and peroxidase substrate (SK-4100), and mounted using VectaMount (H-5000, all from Vector). .. A BX61 motorized system microscope was used to obtain bright-field images.

    Article Title: Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death
    Article Snippet: For detection of ferric iron, DAB-enhanced Perls’ iron staining was performed, as described previously [ ]. .. Briefly, brain sections were incubated in Perls’ solution (Abcam) for 30 min, followed by incubation in 0.05% DAB substrate kit (Vector Laboratories, Burlingame, CA, USA) for 20 min. Then, 1% H2 O2 was added, and samples were incubated for 30 min. .. Sections were then washed, covered with mounting medium, and visualized using a BX51 light microscope (Olympus).

    Plasmid Preparation:

    Article Title: Inhibition of Smooth Muscle Cell Proliferation and Migration by a Talin Modulator Attenuates Neointimal Formation after Femoral Arterial Injury
    Article Snippet: The sections were then incubated with α-smooth muscle actin (α-SMA; A2547, Sigma Aldrich) antibodies for 1 hour at RT and then with biotinylated anti-mouse IgG antibodies (BA-2000, Vector, Burlingame, CA, USA) for 30 minutes at RT. .. Subsequently, the sections were incubated with the ABC reagent (PK-6100) and peroxidase substrate (SK-4100), and mounted using VectaMount (H-5000, all from Vector). .. A BX61 motorized system microscope was used to obtain bright-field images.

    Avidin-Biotin Assay:

    Article Title: Changes of the Structural and Biomechanical Properties of the Bovine Pericardium after the Removal of ?-Gal Epitopes by Decellularization and ?-Galactosidase Treatment
    Article Snippet: The sectioned samples were incubated in 1 ug/mL biotinylated GSIB4 lectin (Vector Lab, Burlingame, CA, USA). .. They were washed with PBS and treated with 5 ug/mL avidin-HRP (Molecular Probes, Eugene, OR, USA), and α-gal epitopes on the valves were visualized using 3,3'-diaminobenzidine (DAB; Vector, Burlingame, CA, USA) as a substrate. .. The DAB staining intensity of the valve tissues was captured under light microscopy.

    Article Title: Glia Maturation Factor and Mitochondrial Uncoupling Proteins 2 and 4 Expression in the Temporal Cortex of Alzheimer’s Disease Brain
    Article Snippet: Anti-UCP2 polyclonal antibody (Calbiochem Millipore, San Diego, CA, USA), Rabbit polyclonal UCP4 antibody (Novus Biologicals, Littleton, CO, USA), anti-rabbit iNOS and rabbit polyclonal to NF-κB p65 (Abcam, Cambridge, MA, USA) were obtained. .. Vectastain avidin biotin peroxidase complex (ABC) reagents and kits (peroxidase mouse IgG/ peroxidase rabbit IgG) and Impact 3,3’-diaminobenzidine (DAB) peroxidase substrate kits were from Vector Labs (Burlingame, CA, USA). .. Additional reagents include phosphate buffered saline (PBS; GIBCO, Life Technologies, Grand Island, NY, USA), Thioflavin-S (Sigma, St Louis, MO, USA).

    Modification:

    Article Title: Hippocampal RAGE Immunoreactivity in Early and Advanced Alzheimer's Disease
    Article Snippet: Non-specific binding sites were blocked by incubation with 5% normal horse serum for two hours at room temperature prior to incubation with the primary antibody (monoclonal mouse anti-human Aβ clone 6E10 antibody, Calbiochem, San Diego, CA, USA) overnight at 4°C. .. The tissue sections were then subjected to a modified ABC technique using the Vectastain Elite ABC Mouse Peroxidase system (Vector Laboratories, Burlingame, CA, USA) with 3,3-diaminobenzidine (Vector Laboratories, Burlingame, CA, USA) as the chromogen. ..

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    Vector Laboratories 3 3 diaminobenzidine
    Sections of human hippocampi (600X) stained with RAGE used in quantitative analyses. The detection system is ABC (Vector Laboratories) with <t>3,3-diaminobenzidine</t> as the chromogen. RAGE immunoreactivity is weak to moderate in hippocampal microvasculature
    3 3 Diaminobenzidine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine - by Bioz Stars, 2021-04
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    Sections of human hippocampi (600X) stained with RAGE used in quantitative analyses. The detection system is ABC (Vector Laboratories) with 3,3-diaminobenzidine as the chromogen. RAGE immunoreactivity is weak to moderate in hippocampal microvasculature

    Journal: Brain research

    Article Title: Hippocampal RAGE Immunoreactivity in Early and Advanced Alzheimer's Disease

    doi: 10.1016/j.brainres.2008.06.124

    Figure Lengend Snippet: Sections of human hippocampi (600X) stained with RAGE used in quantitative analyses. The detection system is ABC (Vector Laboratories) with 3,3-diaminobenzidine as the chromogen. RAGE immunoreactivity is weak to moderate in hippocampal microvasculature

    Article Snippet: The tissue sections were then subjected to a modified ABC technique using the Vectastain Elite ABC Mouse Peroxidase system (Vector Laboratories, Burlingame, CA, USA) with 3,3-diaminobenzidine (Vector Laboratories, Burlingame, CA, USA) as the chromogen.

    Techniques: Staining, Plasmid Preparation

    Brucella melitensis infectious foci in liver. At 3 weeks post-infection, pathogenic B. melitensis -infected cells developed multiple granulomas (A,D; H E staining). Immunohistochemical analysis of bacterial foci was performed using biotinylated primary antibodies (1.0 μg/ml, #TC-7011, Tetracore Inc.), endogenous biotin blocking, and streptavidin-conjugated to horseradish peroxidase (St∼HRP) using 3,3′-diaminobenzidine (DAB) as the final chromogen (B,E; brown precipitate). Staining of normal liver of healthy non-infected mice produced no signal (not shown). Staining of liver tissue at 3 weeks post-infection using the same protocol but substituting biotinylated anti- Brucella antibodies with normal serum produced no signal (F). Immunohistochemical analysis of macrophage lineage cells using antibodies to IBA-1 (dilution 1:500, #019-19741, Wako Chemicals USA, Inc.) followed by HRP rabbit polymer conjugate (#87-9263, Invitrogen Life Technologies) revealed positive staining of cells at the granuloma periphery (C). Residential macrophages – Kupffer cells were also positive for IBA-1 staining (C). Although Brucella was found in the granuloma core, these core cells were basically negative for IBA-1 staining (compare B versus C). Sections were counterstained with hematoxylin (B,C,E,F). Scale bars: 20 μm (A,B,C,E), 50 μm (F) and 200 μm (D).

    Journal: Disease Models & Mechanisms

    Article Title: Osteoarticular tissue infection and development of skeletal pathology in murine brucellosis

    doi: 10.1242/dmm.011056

    Figure Lengend Snippet: Brucella melitensis infectious foci in liver. At 3 weeks post-infection, pathogenic B. melitensis -infected cells developed multiple granulomas (A,D; H E staining). Immunohistochemical analysis of bacterial foci was performed using biotinylated primary antibodies (1.0 μg/ml, #TC-7011, Tetracore Inc.), endogenous biotin blocking, and streptavidin-conjugated to horseradish peroxidase (St∼HRP) using 3,3′-diaminobenzidine (DAB) as the final chromogen (B,E; brown precipitate). Staining of normal liver of healthy non-infected mice produced no signal (not shown). Staining of liver tissue at 3 weeks post-infection using the same protocol but substituting biotinylated anti- Brucella antibodies with normal serum produced no signal (F). Immunohistochemical analysis of macrophage lineage cells using antibodies to IBA-1 (dilution 1:500, #019-19741, Wako Chemicals USA, Inc.) followed by HRP rabbit polymer conjugate (#87-9263, Invitrogen Life Technologies) revealed positive staining of cells at the granuloma periphery (C). Residential macrophages – Kupffer cells were also positive for IBA-1 staining (C). Although Brucella was found in the granuloma core, these core cells were basically negative for IBA-1 staining (compare B versus C). Sections were counterstained with hematoxylin (B,C,E,F). Scale bars: 20 μm (A,B,C,E), 50 μm (F) and 200 μm (D).

    Article Snippet: After three washes with PBST, slides were finally stained with ImmPact 3,3′-diaminobenzidine (DAB; Vector Labs) and counterstained with hematoxylin (Vector Labs) or alcian blue (Thermo Fisher Scientific Inc.).

    Techniques: Infection, Staining, Immunohistochemistry, Blocking Assay, Mouse Assay, Produced

    Detection and in vitro characterization of rSARS‐CoV‐2. ( A ) CPE: At 48 hr after scaling up transfected Vero E6 cells into T‐75 flasks (Fig. 3 ), CPE can be already observed. Representative images of mock‐infected, SARS‐CoV‐2‐infected, and transfected Vero E6 are shown. Scale bars are 400 µm. ( B ) IFA: Vero E6 cells (12‐well plate format, 0.5 × 10 6 cells/well, triplicates) were infected with tissue culture supernatants from transfected Vero E6 cells. Mock‐infected and SARS‐CoV‐2‐infected Vero E6 cells were included as internal controls. At 24 hr post‐infection, cells were fixed with 10% neutral buffered formalin. After fixation for 16 hr, cells were permeabilized with 0.5% Triton X‐100 for 10 min. Cells were then washed three times with 1× PBS and incubated with 1 μg/ml of an anti‐SARS NP MAb 1C7 at 37°C. After 1 hr incubation with the primary NP 1C7 MAb, cells are washed three times with 1× PBS and incubated with an anti‐mouse FITC‐conjugated secondary antibody at 37°C. After 1 hr, cells are washed three times with 1× PBS and observed under a fluorescent microscope. DAPI was used to stain the nucleus. Scale bars are 100 µm. ( C ) Plaque assay: Confluent monolayers of Vero E6 cells (6‐well plate format, 1.2 × 10 6 cells/well, triplicates) were infected with SARS‐CoV‐2 or rSARS‐CoV‐2 at 37°C for 1 hr and overlaid with agar. After 72 hr in a 37°C incubator with 5% CO 2 , cells were fixed in 10% neutral buffered formalin for 16 hr before agar was removed. Next, cells were permeabilized with 0.5% Triton X‐100 for 10 min and prepared for immunostaining as previously described using the anti‐NP MAb (1C7) and vector kits (Vectastain ABC kit and DAB HRP substrate kit; Vector Laboratories). ( D ) Viral growth kinetics: Vero E6 cells (12‐well plate format, 0.5 × 10 6 cells/well, triplicates) were infected (MOI of 0.01) with SARS‐CoV‐2 (black) or rSARS‐CoV‐2 (red) and placed in a 37°C incubator with 5% CO 2 for 4 days. At 12, 24, 48, 72, and 96 hr post‐infection, viral titers in supernatants were determined by plaque assay (PFU/ml). Error bars indicate the standard deviations from three separate experiments. The dashed black line indicates the limit of detection (10 PFU/ml).

    Journal: Current Protocols in Microbiology

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome). Generation of recombinant SARS‐CoV‐2 using a bacterial artificial chromosome

    doi: 10.1002/cpmc.126

    Figure Lengend Snippet: Detection and in vitro characterization of rSARS‐CoV‐2. ( A ) CPE: At 48 hr after scaling up transfected Vero E6 cells into T‐75 flasks (Fig. 3 ), CPE can be already observed. Representative images of mock‐infected, SARS‐CoV‐2‐infected, and transfected Vero E6 are shown. Scale bars are 400 µm. ( B ) IFA: Vero E6 cells (12‐well plate format, 0.5 × 10 6 cells/well, triplicates) were infected with tissue culture supernatants from transfected Vero E6 cells. Mock‐infected and SARS‐CoV‐2‐infected Vero E6 cells were included as internal controls. At 24 hr post‐infection, cells were fixed with 10% neutral buffered formalin. After fixation for 16 hr, cells were permeabilized with 0.5% Triton X‐100 for 10 min. Cells were then washed three times with 1× PBS and incubated with 1 μg/ml of an anti‐SARS NP MAb 1C7 at 37°C. After 1 hr incubation with the primary NP 1C7 MAb, cells are washed three times with 1× PBS and incubated with an anti‐mouse FITC‐conjugated secondary antibody at 37°C. After 1 hr, cells are washed three times with 1× PBS and observed under a fluorescent microscope. DAPI was used to stain the nucleus. Scale bars are 100 µm. ( C ) Plaque assay: Confluent monolayers of Vero E6 cells (6‐well plate format, 1.2 × 10 6 cells/well, triplicates) were infected with SARS‐CoV‐2 or rSARS‐CoV‐2 at 37°C for 1 hr and overlaid with agar. After 72 hr in a 37°C incubator with 5% CO 2 , cells were fixed in 10% neutral buffered formalin for 16 hr before agar was removed. Next, cells were permeabilized with 0.5% Triton X‐100 for 10 min and prepared for immunostaining as previously described using the anti‐NP MAb (1C7) and vector kits (Vectastain ABC kit and DAB HRP substrate kit; Vector Laboratories). ( D ) Viral growth kinetics: Vero E6 cells (12‐well plate format, 0.5 × 10 6 cells/well, triplicates) were infected (MOI of 0.01) with SARS‐CoV‐2 (black) or rSARS‐CoV‐2 (red) and placed in a 37°C incubator with 5% CO 2 for 4 days. At 12, 24, 48, 72, and 96 hr post‐infection, viral titers in supernatants were determined by plaque assay (PFU/ml). Error bars indicate the standard deviations from three separate experiments. The dashed black line indicates the limit of detection (10 PFU/ml).

    Article Snippet: DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100).

    Techniques: In Vitro, Transfection, Infection, Immunofluorescence, Incubation, Microscopy, Staining, Plaque Assay, Immunostaining, Plasmid Preparation

    Autoimmune reactions against spinal cord cells. Lumbar spinal cord sections of control mice were reacted with a 1:20 dilution of the serum from a control ( A ) or IL3-Tg mouse ( B – D ). Detection of primary antibodies was done by using the avidin-biotin-peroxidase technique and diaminobenzidine. C and D are higher magnification views (×40) of areas highlighted by the left or right rectangle, respectively (×10). Sensory neurons ( C ) and MN ( D ) are the main targets of the autoimmune reaction.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Transgenic mice for interleukin 3 develop motor neuron degeneration associated with autoimmune reaction against spinal cord motor neurons

    doi:

    Figure Lengend Snippet: Autoimmune reactions against spinal cord cells. Lumbar spinal cord sections of control mice were reacted with a 1:20 dilution of the serum from a control ( A ) or IL3-Tg mouse ( B – D ). Detection of primary antibodies was done by using the avidin-biotin-peroxidase technique and diaminobenzidine. C and D are higher magnification views (×40) of areas highlighted by the left or right rectangle, respectively (×10). Sensory neurons ( C ) and MN ( D ) are the main targets of the autoimmune reaction.

    Article Snippet: The detection of primary antibodies was performed by using avidin-biotin-peroxidase techniques and diaminobenzidine (Vector Laboratories).

    Techniques: Mouse Assay, Avidin-Biotin Assay