diaminobenzidine  (Thermo Fisher)


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    Structured Review

    Thermo Fisher diaminobenzidine
    Diaminobenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diaminobenzidine/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
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    diaminobenzidine - by Bioz Stars, 2021-03
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    Related Articles

    Incubation:

    Article Title: ACh-induced endothelial NO synthase translocation, NO release and vasodilatation in the hamster microcirculation in vivo
    Article Snippet: Each section was incubated separately with anti-eNOS (high mol. mass) or anti-Cav-1 (low mol. mass) primary antibodies (1:2500), for 3 h at room temperature. .. This was followed by 1 h incubation with the appropriate conjugated secondary antibody and developed by a 15 min incubation with 0.01 % 3,3′-diaminobenzidine, 0.5 % H2 O2 in the dark, or by chemiluminescence (SuperSignal, WestFemto, Pierce, Rockford, IL, USA). .. Additional Western blots were performed with similar procedures, using primary antibodies for β-COP and Na+ ,K+ -ATPase (1:1000), to determine the presence of these protein markers of Golgi and plasma membranes, respectively.

    Article Title: Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway.
    Article Snippet: The membranes were blocked for 1 h in Tris-buffered saline (TBS) containing 5 % non-fat dry milk at room temperature (phosphorylated proteins were blocked in 5 % BSA), and incubated with the primary antibodies at 4°C overnight. .. After washing three times with TBST, the membranes were incubated with HRP-conjugated goat anti-rabbit IgG (Sangon Biotech) at 37°C for 1 h. The proteins were visualized using 3,3′-diaminobenzidine, and detected by enhanced chemiluminescence (ECL; Thermo Scientific) and autoradiography. .. RT-qPCRThe total RNA was extracted using RNAiso plus (Invitrogen, USA) reagent and subjected to reverse transcription with 5× PrimeScript RT Master Mix (Promega, USA).

    Article Title: In vitro and in vivo anti-tumor activity of CoQ0 against melanoma cells: inhibition of metastasis and induction of cell-cycle arrest and apoptosis through modulation of Wnt/β-catenin signaling pathways
    Article Snippet: The slides were incubated with biotinylated secondary antibody (Zymed Laboratories, South San Francisco, CA) for 20 min at room temperature. .. Finally, slides were incubated with avidin-biotin complex reagent and stained with 3,3′-diaminobenzidine according to the manufacturer's protocol (Histostain® -Plus Kit, Zymed Laboratories). .. For western blot, tumor tissues were homogenized in RIPA buffer containing 1% protease inhibitor cocktail and 1% phosphatase inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and samples (50 μg of protein) were subjected to electrophoresis on SDS gels (8-10%), transferred to a PVDF membrane.

    Staining:

    Article Title: Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of Corchorus olitorius L. on Spheroid Proliferation
    Article Snippet: Biotinylated secondary antibody and streptavidin-peroxidase (Histostain-Plus, IHC Kit, HRP, 859043, Thermo Fischer) were added and each secondary antibody was incubated for 30 min followed by PBS wash (×3) for 5 min. .. Cells were then stained with diaminobenzidine for 5 min for enhancement of immunolabeling. .. After being washed with distilled water, they were counterstained with Mayer’s hematoxylin for 5 min and mounted with mounting medium (Merck Millipore, 107961, Germany).

    Article Title: In vitro and in vivo anti-tumor activity of CoQ0 against melanoma cells: inhibition of metastasis and induction of cell-cycle arrest and apoptosis through modulation of Wnt/β-catenin signaling pathways
    Article Snippet: The slides were incubated with biotinylated secondary antibody (Zymed Laboratories, South San Francisco, CA) for 20 min at room temperature. .. Finally, slides were incubated with avidin-biotin complex reagent and stained with 3,3′-diaminobenzidine according to the manufacturer's protocol (Histostain® -Plus Kit, Zymed Laboratories). .. For western blot, tumor tissues were homogenized in RIPA buffer containing 1% protease inhibitor cocktail and 1% phosphatase inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and samples (50 μg of protein) were subjected to electrophoresis on SDS gels (8-10%), transferred to a PVDF membrane.

    Immunolabeling:

    Article Title: Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of Corchorus olitorius L. on Spheroid Proliferation
    Article Snippet: Biotinylated secondary antibody and streptavidin-peroxidase (Histostain-Plus, IHC Kit, HRP, 859043, Thermo Fischer) were added and each secondary antibody was incubated for 30 min followed by PBS wash (×3) for 5 min. .. Cells were then stained with diaminobenzidine for 5 min for enhancement of immunolabeling. .. After being washed with distilled water, they were counterstained with Mayer’s hematoxylin for 5 min and mounted with mounting medium (Merck Millipore, 107961, Germany).

    Autoradiography:

    Article Title: Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway.
    Article Snippet: The membranes were blocked for 1 h in Tris-buffered saline (TBS) containing 5 % non-fat dry milk at room temperature (phosphorylated proteins were blocked in 5 % BSA), and incubated with the primary antibodies at 4°C overnight. .. After washing three times with TBST, the membranes were incubated with HRP-conjugated goat anti-rabbit IgG (Sangon Biotech) at 37°C for 1 h. The proteins were visualized using 3,3′-diaminobenzidine, and detected by enhanced chemiluminescence (ECL; Thermo Scientific) and autoradiography. .. RT-qPCRThe total RNA was extracted using RNAiso plus (Invitrogen, USA) reagent and subjected to reverse transcription with 5× PrimeScript RT Master Mix (Promega, USA).

    other:

    Article Title: Pathological TDP-43 distinguishes sporadic amyotrophic lateral sclerosis from amyotrophic lateral sclerosis with SOD1 mutations.
    Article Snippet: Amyotrophic lateral sclerosis (ALS) is a common, fatal motor neuron disorder with no effective treatment.

    Avidin-Biotin Assay:

    Article Title: In vitro and in vivo anti-tumor activity of CoQ0 against melanoma cells: inhibition of metastasis and induction of cell-cycle arrest and apoptosis through modulation of Wnt/β-catenin signaling pathways
    Article Snippet: The slides were incubated with biotinylated secondary antibody (Zymed Laboratories, South San Francisco, CA) for 20 min at room temperature. .. Finally, slides were incubated with avidin-biotin complex reagent and stained with 3,3′-diaminobenzidine according to the manufacturer's protocol (Histostain® -Plus Kit, Zymed Laboratories). .. For western blot, tumor tissues were homogenized in RIPA buffer containing 1% protease inhibitor cocktail and 1% phosphatase inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and samples (50 μg of protein) were subjected to electrophoresis on SDS gels (8-10%), transferred to a PVDF membrane.

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  • 86
    Thermo Fisher diaminobenzidine substrate chromogen
    Effects of Aks depletion on the immunohistochemical expression of tumor-associated factors. DEN-induced adenomas had relatively low numbers of proliferating ki-67-positive cells and occasional apoptotic Caspase-3-positive cells (arrows). The depletion of Aks had no effect on proliferation and apoptosis of neoplastic cells. Likewise, it did not alter the expression of tumor-associated proteins NFκ-B p65 and c-Jun. NFκ-B p65 expression in tumor cells remains cytoplasmic, whereas non-parenchymal cells morphologically consistent with kupffer cells show nuclear expression. IHC; <t>Diaminobenzidine</t> chromogen, Hematoxylin counterstain. Scale bars: 50 μm. Numbers on the y-axis of bar graphs correspond to the mean±SEM of the parameters assessed. NS p > 0.05
    Diaminobenzidine Substrate Chromogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diaminobenzidine substrate chromogen/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine substrate chromogen - by Bioz Stars, 2021-03
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    99
    Thermo Fisher 3 3 diaminobenzidine dab
    Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) <t>3,3′</t> <t>diaminobenzidine</t> <t>(DAB),</t> used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.
    3 3 Diaminobenzidine Dab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
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    95
    Thermo Fisher metal enhanced diaminobenzidine substrate
    Quantitative immunohistochemical analysis of H + /K + -ATPase in LN sections of untreated animals and animals with AIG. (a) Anti–H + /K + -ATPase FITC-stained LN sections of untreated BALB/c mice were incubated with secondary anti-FITC HRPO Ab and developed using enhanced <t>diaminobenzidine</t> substrate. Pictures were taken with the bright field setting of the microscope (top) and additionally analyzed using the public domain NIH Image program. Single peaks represent positive staining for H + /K + -ATPase in the threshold histogram plot profile (top) and in the surface plot view (bottom) and can be detected in gastric LN, but not peripheral LN, sections of untreated animals. (b) In animals with AIG, an increase in the staining frequency for H + /K + -ATPase was observed in gastric LN (gastric LN AIG), but not in peripheral LN (peripheral LN AIG), sections.
    Metal Enhanced Diaminobenzidine Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/metal enhanced diaminobenzidine substrate/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    metal enhanced diaminobenzidine substrate - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    Image Search Results


    Effects of Aks depletion on the immunohistochemical expression of tumor-associated factors. DEN-induced adenomas had relatively low numbers of proliferating ki-67-positive cells and occasional apoptotic Caspase-3-positive cells (arrows). The depletion of Aks had no effect on proliferation and apoptosis of neoplastic cells. Likewise, it did not alter the expression of tumor-associated proteins NFκ-B p65 and c-Jun. NFκ-B p65 expression in tumor cells remains cytoplasmic, whereas non-parenchymal cells morphologically consistent with kupffer cells show nuclear expression. IHC; Diaminobenzidine chromogen, Hematoxylin counterstain. Scale bars: 50 μm. Numbers on the y-axis of bar graphs correspond to the mean±SEM of the parameters assessed. NS p > 0.05

    Journal: Journal of Cancer

    Article Title: Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib

    doi: 10.7150/jca.22329

    Figure Lengend Snippet: Effects of Aks depletion on the immunohistochemical expression of tumor-associated factors. DEN-induced adenomas had relatively low numbers of proliferating ki-67-positive cells and occasional apoptotic Caspase-3-positive cells (arrows). The depletion of Aks had no effect on proliferation and apoptosis of neoplastic cells. Likewise, it did not alter the expression of tumor-associated proteins NFκ-B p65 and c-Jun. NFκ-B p65 expression in tumor cells remains cytoplasmic, whereas non-parenchymal cells morphologically consistent with kupffer cells show nuclear expression. IHC; Diaminobenzidine chromogen, Hematoxylin counterstain. Scale bars: 50 μm. Numbers on the y-axis of bar graphs correspond to the mean±SEM of the parameters assessed. NS p > 0.05

    Article Snippet: Color was developed with Diaminobenzidine substrate-chromogen (ThermoFisher Scientific/Lab Vision) and tissues were counterstained with hematoxylin.

    Techniques: Immunohistochemistry, Expressing

    Effects of Aks depletion on the immunohistochemical expression of neoplastic cell Wnt/β-catenin signaling. Hepatocellular adenomas show increased β-catenin immunohistochemical signal. However, β-catenin remains localized in cell membranes with only few cells showing cytoplasmic stabilization and none nuclear translocation of β-catenin. The tumor cells also show prominent cytoplasmic DKK1 expression. Anti-Aks therapy affects neither β-catenin, nor DKK1 expression in liver tumors. IHC; Diaminobenzidine chromogen, Hematoxylin counterstain. Scale bars: 50 μm. Numbers on the y-axis of bar graphs correspond to the mean±SEM of the parameters assessed. NS p > 0.05

    Journal: Journal of Cancer

    Article Title: Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib

    doi: 10.7150/jca.22329

    Figure Lengend Snippet: Effects of Aks depletion on the immunohistochemical expression of neoplastic cell Wnt/β-catenin signaling. Hepatocellular adenomas show increased β-catenin immunohistochemical signal. However, β-catenin remains localized in cell membranes with only few cells showing cytoplasmic stabilization and none nuclear translocation of β-catenin. The tumor cells also show prominent cytoplasmic DKK1 expression. Anti-Aks therapy affects neither β-catenin, nor DKK1 expression in liver tumors. IHC; Diaminobenzidine chromogen, Hematoxylin counterstain. Scale bars: 50 μm. Numbers on the y-axis of bar graphs correspond to the mean±SEM of the parameters assessed. NS p > 0.05

    Article Snippet: Color was developed with Diaminobenzidine substrate-chromogen (ThermoFisher Scientific/Lab Vision) and tissues were counterstained with hematoxylin.

    Techniques: Immunohistochemistry, Expressing, Translocation Assay

    Effects of Aks depletion on INCENP expression and on blood serum tumor markers. (A) Hepatocellular adenomas show ample nuclear INCENP. In the tumors of anti-Aks-treated mice, however, INCENP expression is practically absent. (B) Mice with DEN-induced hepatocellular adenomas have significantly less Bcl-2 in their blood serum by comparison with tumor-free controls. The levels of Bcl-2 increase significantly after anti-Aks treatment. (C) Serum c-met is significantly increased after the neutralization of Aks. IHC; Diaminobenzidine chromogen, Hematoxylin counterstain. Scale bars: 25 μm. Numbers on the y-axis of bar graphs correspond to the mean±SEM of the parameters assessed. NS p > 0.05, *p

    Journal: Journal of Cancer

    Article Title: Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib

    doi: 10.7150/jca.22329

    Figure Lengend Snippet: Effects of Aks depletion on INCENP expression and on blood serum tumor markers. (A) Hepatocellular adenomas show ample nuclear INCENP. In the tumors of anti-Aks-treated mice, however, INCENP expression is practically absent. (B) Mice with DEN-induced hepatocellular adenomas have significantly less Bcl-2 in their blood serum by comparison with tumor-free controls. The levels of Bcl-2 increase significantly after anti-Aks treatment. (C) Serum c-met is significantly increased after the neutralization of Aks. IHC; Diaminobenzidine chromogen, Hematoxylin counterstain. Scale bars: 25 μm. Numbers on the y-axis of bar graphs correspond to the mean±SEM of the parameters assessed. NS p > 0.05, *p

    Article Snippet: Color was developed with Diaminobenzidine substrate-chromogen (ThermoFisher Scientific/Lab Vision) and tissues were counterstained with hematoxylin.

    Techniques: Expressing, Mouse Assay, Neutralization, Immunohistochemistry

    Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) 3,3′ diaminobenzidine (DAB), used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.

    Journal: The European Journal of Neuroscience

    Article Title: Central nervous system neurons acquire mast cell products via transgranulation

    doi: 10.1111/j.1460-9568.2005.04429.x

    Figure Lengend Snippet: Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) 3,3′ diaminobenzidine (DAB), used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.

    Article Snippet: The first made use of conventional staining with 3,3′ diaminobenzidine (DAB) (Immunopure DAB, Pierce) and the second, a silver intensification followed by gold toning of the DAB product (not shown).

    Techniques: Derivative Assay, Binding Assay

    Cell surface expression of B5 in porcine cells. (A) Cartoon of the predicted structure of B5 as an integral membrane protein with a predicted C-terminal α-helix. The C terminus and N terminus are labeled. (B) Cells transiently transfected with pB5-myc or vector only (pcDNA-myc) fixed at 48 h posttransfection and stained with anti-myc antibody, a secondary, peroxidase-conjugated antibody and diaminobenzidine substrate. (C) FACS of SK6-A7 cells transiently transfected with pcDNA vector (left) or pB5-myc (right) and stained with anti-myc (9E10) and a secondary, FITC-conjugated antibody (gray lines). Black peaks are cells in the absence of primary anti-myc antibody. (D) FACS of the indicated stable porcine cell line exposed to anti-Xpress and a secondary, FITC-conjugated antibody. Cells were permeabilized (P) with methanol-acetone or not permeabilized (unpermeabilized [U]) before staining. Neo is a vector-only-transformed cell line. NB5 is a stable cell line made with an N-terminal Xpress epitope-tagged B5. HB1-9 is a stable untagged HVEM-expressing porcine cell line. (E and F) NB5 and HB1-9 cells surface labeled with biotin and lysed as described in Materials and Methods. Lysates normalized for protein concentration were immunoprecipitated with preimmune serum (Pre) or with anti-HVEM polyclonal serum (HVEM), or anti-Xpress monoclonal antibody (XPRS). Proteins visualized in Western blots hybridized with streptavidin-conjugated peroxidase and enhanced chemiluminescence substrate (Amersham) (E) or anti-Xpress monoclonal antibody and alkaline phosphatase-conjugated anti-mouse secondary antibody and NBT/BCIP (F). The positions of molecular mass markers (in kilodaltons) are shown to the left of the gels. The arrow points to B5 at about 43 kDa.

    Journal: Journal of Virology

    Article Title: A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins

    doi: 10.1128/JVI.79.12.7419-7430.2005

    Figure Lengend Snippet: Cell surface expression of B5 in porcine cells. (A) Cartoon of the predicted structure of B5 as an integral membrane protein with a predicted C-terminal α-helix. The C terminus and N terminus are labeled. (B) Cells transiently transfected with pB5-myc or vector only (pcDNA-myc) fixed at 48 h posttransfection and stained with anti-myc antibody, a secondary, peroxidase-conjugated antibody and diaminobenzidine substrate. (C) FACS of SK6-A7 cells transiently transfected with pcDNA vector (left) or pB5-myc (right) and stained with anti-myc (9E10) and a secondary, FITC-conjugated antibody (gray lines). Black peaks are cells in the absence of primary anti-myc antibody. (D) FACS of the indicated stable porcine cell line exposed to anti-Xpress and a secondary, FITC-conjugated antibody. Cells were permeabilized (P) with methanol-acetone or not permeabilized (unpermeabilized [U]) before staining. Neo is a vector-only-transformed cell line. NB5 is a stable cell line made with an N-terminal Xpress epitope-tagged B5. HB1-9 is a stable untagged HVEM-expressing porcine cell line. (E and F) NB5 and HB1-9 cells surface labeled with biotin and lysed as described in Materials and Methods. Lysates normalized for protein concentration were immunoprecipitated with preimmune serum (Pre) or with anti-HVEM polyclonal serum (HVEM), or anti-Xpress monoclonal antibody (XPRS). Proteins visualized in Western blots hybridized with streptavidin-conjugated peroxidase and enhanced chemiluminescence substrate (Amersham) (E) or anti-Xpress monoclonal antibody and alkaline phosphatase-conjugated anti-mouse secondary antibody and NBT/BCIP (F). The positions of molecular mass markers (in kilodaltons) are shown to the left of the gels. The arrow points to B5 at about 43 kDa.

    Article Snippet: For immunostaining, cells at about 100% confluency were fixed with 2% paraformaldehyde-0.2% glutaraldehyde and stained with anti-myc (1:1,000) followed by anti-mouse secondary antibody and diaminobenzidine substrate (Lifetech).

    Techniques: Expressing, Labeling, Transfection, Plasmid Preparation, Staining, FACS, Transformation Assay, Stable Transfection, Protein Concentration, Immunoprecipitation, Western Blot

    Quantitative immunohistochemical analysis of H + /K + -ATPase in LN sections of untreated animals and animals with AIG. (a) Anti–H + /K + -ATPase FITC-stained LN sections of untreated BALB/c mice were incubated with secondary anti-FITC HRPO Ab and developed using enhanced diaminobenzidine substrate. Pictures were taken with the bright field setting of the microscope (top) and additionally analyzed using the public domain NIH Image program. Single peaks represent positive staining for H + /K + -ATPase in the threshold histogram plot profile (top) and in the surface plot view (bottom) and can be detected in gastric LN, but not peripheral LN, sections of untreated animals. (b) In animals with AIG, an increase in the staining frequency for H + /K + -ATPase was observed in gastric LN (gastric LN AIG), but not in peripheral LN (peripheral LN AIG), sections.

    Journal: The Journal of Experimental Medicine

    Article Title: Constitutive Presentation of a Natural Tissue Autoantigen Exclusively by Dendritic Cells in the Draining Lymph Node

    doi: 10.1084/jem.20020991

    Figure Lengend Snippet: Quantitative immunohistochemical analysis of H + /K + -ATPase in LN sections of untreated animals and animals with AIG. (a) Anti–H + /K + -ATPase FITC-stained LN sections of untreated BALB/c mice were incubated with secondary anti-FITC HRPO Ab and developed using enhanced diaminobenzidine substrate. Pictures were taken with the bright field setting of the microscope (top) and additionally analyzed using the public domain NIH Image program. Single peaks represent positive staining for H + /K + -ATPase in the threshold histogram plot profile (top) and in the surface plot view (bottom) and can be detected in gastric LN, but not peripheral LN, sections of untreated animals. (b) In animals with AIG, an increase in the staining frequency for H + /K + -ATPase was observed in gastric LN (gastric LN AIG), but not in peripheral LN (peripheral LN AIG), sections.

    Article Snippet: HRPO localization was revealed using a metal-enhanced diaminobenzidine substrate (Pierce Chemical Co.).

    Techniques: Immunohistochemistry, Staining, Mouse Assay, Incubation, Microscopy