diaminobenzidine (Thermo Fisher)
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Thermo Fisher
diaminobenzidine
Diaminobenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diaminobenzidine/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Diaminobenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diaminobenzidine/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
diaminobenzidine - by Bioz Stars,
2021-03
86/100 stars
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Incubation:Article Title: ACh-induced endothelial NO synthase translocation, NO release and vasodilatation in the hamster microcirculation in vivo Article Snippet: Each section was incubated separately with anti-eNOS (high mol. mass) or anti-Cav-1 (low mol. mass) primary antibodies (1:2500), for 3 h at room temperature. .. This was followed by 1 h incubation with the appropriate conjugated secondary antibody and developed by a 15 min incubation with 0.01 % Article Title: Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway. Article Snippet: The membranes were blocked for 1 h in Tris-buffered saline (TBS) containing 5 % non-fat dry milk at room temperature (phosphorylated proteins were blocked in 5 % BSA), and incubated with the primary antibodies at 4°C overnight. .. After washing three times with TBST, the membranes were incubated with HRP-conjugated goat anti-rabbit IgG (Sangon Biotech) at 37°C for 1 h. The proteins were visualized using Article Title: In vitro and in vivo anti-tumor activity of CoQ0 against melanoma cells: inhibition of metastasis and induction of cell-cycle arrest and apoptosis through modulation of Wnt/β-catenin signaling pathways Article Snippet: The slides were incubated with biotinylated secondary antibody (Zymed Laboratories, South San Francisco, CA) for 20 min at room temperature. .. Finally, slides were incubated with avidin-biotin complex reagent and stained with Staining:Article Title: Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of Corchorus olitorius L. on Spheroid Proliferation Article Snippet: Biotinylated secondary antibody and streptavidin-peroxidase (Histostain-Plus, IHC Kit, HRP, 859043, Thermo Fischer) were added and each secondary antibody was incubated for 30 min followed by PBS wash (×3) for 5 min. .. Cells were then stained with Article Title: In vitro and in vivo anti-tumor activity of CoQ0 against melanoma cells: inhibition of metastasis and induction of cell-cycle arrest and apoptosis through modulation of Wnt/β-catenin signaling pathways Article Snippet: The slides were incubated with biotinylated secondary antibody (Zymed Laboratories, South San Francisco, CA) for 20 min at room temperature. .. Finally, slides were incubated with avidin-biotin complex reagent and stained with Immunolabeling:Article Title: Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of Corchorus olitorius L. on Spheroid Proliferation Article Snippet: Biotinylated secondary antibody and streptavidin-peroxidase (Histostain-Plus, IHC Kit, HRP, 859043, Thermo Fischer) were added and each secondary antibody was incubated for 30 min followed by PBS wash (×3) for 5 min. .. Cells were then stained with Autoradiography:Article Title: Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway. Article Snippet: The membranes were blocked for 1 h in Tris-buffered saline (TBS) containing 5 % non-fat dry milk at room temperature (phosphorylated proteins were blocked in 5 % BSA), and incubated with the primary antibodies at 4°C overnight. .. After washing three times with TBST, the membranes were incubated with HRP-conjugated goat anti-rabbit IgG (Sangon Biotech) at 37°C for 1 h. The proteins were visualized using other:Article Title: Pathological TDP-43 distinguishes sporadic amyotrophic lateral sclerosis from amyotrophic lateral sclerosis with SOD1 mutations. Article Snippet: Amyotrophic lateral sclerosis (ALS) is a common, fatal motor neuron disorder with no effective treatment. Avidin-Biotin Assay:Article Title: In vitro and in vivo anti-tumor activity of CoQ0 against melanoma cells: inhibition of metastasis and induction of cell-cycle arrest and apoptosis through modulation of Wnt/β-catenin signaling pathways Article Snippet: The slides were incubated with biotinylated secondary antibody (Zymed Laboratories, South San Francisco, CA) for 20 min at room temperature. .. Finally, slides were incubated with avidin-biotin complex reagent and stained with |
![Cell surface expression of B5 in porcine cells. (A) Cartoon of the predicted structure of B5 as an integral membrane protein with a predicted C-terminal α-helix. The C terminus and N terminus are labeled. (B) Cells transiently transfected with pB5-myc or vector only (pcDNA-myc) fixed at 48 h posttransfection and stained with anti-myc antibody, a secondary, peroxidase-conjugated antibody and diaminobenzidine substrate. (C) FACS of SK6-A7 cells transiently transfected with pcDNA vector (left) or pB5-myc (right) and stained with anti-myc (9E10) and a secondary, FITC-conjugated antibody (gray lines). Black peaks are cells in the absence of primary anti-myc antibody. (D) FACS of the indicated stable porcine cell line exposed to anti-Xpress and a secondary, FITC-conjugated antibody. Cells were permeabilized (P) with methanol-acetone or not permeabilized (unpermeabilized [U]) before staining. Neo is a vector-only-transformed cell line. NB5 is a stable cell line made with an N-terminal Xpress epitope-tagged B5. HB1-9 is a stable untagged HVEM-expressing porcine cell line. (E and F) NB5 and HB1-9 cells surface labeled with biotin and lysed as described in Materials and Methods. Lysates normalized for protein concentration were immunoprecipitated with preimmune serum (Pre) or with anti-HVEM polyclonal serum (HVEM), or anti-Xpress monoclonal antibody (XPRS). Proteins visualized in Western blots hybridized with streptavidin-conjugated peroxidase and enhanced chemiluminescence substrate (Amersham) (E) or anti-Xpress monoclonal antibody and alkaline phosphatase-conjugated anti-mouse secondary antibody and NBT/BCIP (F). The positions of molecular mass markers (in kilodaltons) are shown to the left of the gels. The arrow points to B5 at about 43 kDa.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1143644/bin/zjv0120563430002.jpg)