diaminobenzidine  (Agilent technologies)

 
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    Structured Review

    Agilent technologies diaminobenzidine
    Immunohistochemical staining of βII-tubulin in colorectal cancer specimen detected by peroxidase-mediated <t>diaminobenzidine</t> (DAB)-staining (brown) with arrows indicating epithelial compartments of colorectal cancer (CRC) (1) and stroma (2). ( A ) Epithelial compartment of tumor cells showing moderate cytoplasmic and more intense nuclear βII-tubulin staining. There are single positive stromal cells (original magnification ×200). ( B ) Adenocarcinoma at lower magnification (original magnification ×100). There are single positive stromal cells. ( C ) Normal colonic mucosa (left) adjacent to tumor complexes (right) showing appearance of positive nuclear βII staining (original magnification ×200).
    Diaminobenzidine, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diaminobenzidine/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer"

    Article Title: Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer

    Journal: Cells

    doi: 10.3390/cells8010025

    Immunohistochemical staining of βII-tubulin in colorectal cancer specimen detected by peroxidase-mediated diaminobenzidine (DAB)-staining (brown) with arrows indicating epithelial compartments of colorectal cancer (CRC) (1) and stroma (2). ( A ) Epithelial compartment of tumor cells showing moderate cytoplasmic and more intense nuclear βII-tubulin staining. There are single positive stromal cells (original magnification ×200). ( B ) Adenocarcinoma at lower magnification (original magnification ×100). There are single positive stromal cells. ( C ) Normal colonic mucosa (left) adjacent to tumor complexes (right) showing appearance of positive nuclear βII staining (original magnification ×200).
    Figure Legend Snippet: Immunohistochemical staining of βII-tubulin in colorectal cancer specimen detected by peroxidase-mediated diaminobenzidine (DAB)-staining (brown) with arrows indicating epithelial compartments of colorectal cancer (CRC) (1) and stroma (2). ( A ) Epithelial compartment of tumor cells showing moderate cytoplasmic and more intense nuclear βII-tubulin staining. There are single positive stromal cells (original magnification ×200). ( B ) Adenocarcinoma at lower magnification (original magnification ×100). There are single positive stromal cells. ( C ) Normal colonic mucosa (left) adjacent to tumor complexes (right) showing appearance of positive nuclear βII staining (original magnification ×200).

    Techniques Used: Immunohistochemistry, Staining

    2) Product Images from "Possible Implication of Local Immune Response in Darier's Disease: An Immunohistochemical Characterization of Lesional Inflammatory Infiltrate"

    Article Title: Possible Implication of Local Immune Response in Darier's Disease: An Immunohistochemical Characterization of Lesional Inflammatory Infiltrate

    Journal: Mediators of Inflammation

    doi: 10.1155/2010/350304

    Few CD1a+ LCs (an intraepidermal CD1a+ LC is indicated by the arrow), small in size, and without evident dendrites, in two cases of Darier's disease ((a), (b)). Normal skin showing more numerous, larger CD1a+ LCs, with long dendrites (c). An increased number of both epidermal and dermal CD1a+ LCs in a case of lichen ruber planus (d). Immunohistochemistry. Original magnification, ×200; Scale bar = 75 μ m; Chromogen: fuchsin ((a), (b)); diaminobenzidine ((c), (d)).
    Figure Legend Snippet: Few CD1a+ LCs (an intraepidermal CD1a+ LC is indicated by the arrow), small in size, and without evident dendrites, in two cases of Darier's disease ((a), (b)). Normal skin showing more numerous, larger CD1a+ LCs, with long dendrites (c). An increased number of both epidermal and dermal CD1a+ LCs in a case of lichen ruber planus (d). Immunohistochemistry. Original magnification, ×200; Scale bar = 75 μ m; Chromogen: fuchsin ((a), (b)); diaminobenzidine ((c), (d)).

    Techniques Used: Immunohistochemistry

    Related Articles

    other:

    Article Title: Twist1 Is Up-Regulated in Gastric Cancer-Associated Fibroblasts with Poor Clinical Outcomes
    Article Snippet: The chromogen used was 3,3′-diaminobenzidine (Dako).

    Article Title: Oncolytic reovirus inhibits angiogenesis through induction of CXCL10/IP-10 and abrogation of HIF activity in soft tissue sarcomas
    Article Snippet: Positive reactions were visualized using 3,3'-diaminobenzidine diaminobenzidine (Dako, Carpinteria, CA) for 10 min.

    Article Title: Promotion of Liver and Lung Tumorigenesis in DEN-Treated Cytoglobin-Deficient Mice
    Article Snippet: 3,3′-Diaminobenzidine (Dako) was used as the chromogen.

    Article Title: Prognostic significance of a component of the Hippo pathway, TAZ, in human uterine endometrioid adenocarcinoma
    Article Snippet: 3,3′-Diaminobenzidine (Dako) was used as a chromogen.

    Article Title: Ferritin Enhances SPIO Tracking of C6 Rat Glioma Cells by MRI
    Article Snippet: Color development was performed with 3,3′-diaminobenzidine (Dako).

    Activity Assay:

    Article Title: Lung dysfunction causes systemic hypoxia in estrogen receptor ? knockout (ER?−/−) mice
    Article Snippet: The Vectastain ABC kit (Vector Laboratories) was used for the avidin–biotin complex (ABC) method according to the manufacturer's instructions. .. Peroxidase activity was visualized with 3,3′-diaminobenzidine (DAKO). ..

    Article Title: Sex differences and effects of oestrogen in rat gastric mucosal defence
    Article Snippet: The Vectastain avidin-biotin complex (ABC) kit (Vector) was used for the ABC method according to the manufacturers' instructions. .. Peroxidase activity was visualized with 3,3'-diaminobenzidine (DAKO). .. Immunohistochemical assessment: Immunohistochemical slides were evaluated by YO, blinded to animal sex status, using light microscopy.

    Article Title: Early onset of puberty and early ovarian failure in CYP7B1 knockout mice
    Article Snippet: The Vectastain ABC kit (Vector Laboratories) was used for ABC method following the manufacturer's instructions. .. Peroxidase activity was visualized with 3,3′-diaminobenzidine (Dako). .. For proliferation studies, BrdUrd was injected s.c. (100 mg/kg of body weight) 2 h before death.

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    • 3 3 diaminobenzidine dab substrate  (Agilent technologies)
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    Agilent technologies 3 3 diaminobenzidine dab substrate
    Image analysis workflow. Quantification of mesenchymal stromal cell/pericyte markers was performed using the CellProfiler image analysis software, and workflows were established for each respective antibody (representative CD146 and CD73 are shown). Fields of view were taken from the original image (Raw) and using the program threshold algorithms <t>DAB</t> stained regions (DAB regions) and nuclei (Nuclei) were defined (colored objects were arbitrarily assigned). Pixel area was determined from the colored segments detected in the DAB regions and nuclei. The images represent individual areas detected with either DAB or nuclei staining. These identified regions were subsequently overlaid on the original field of view to allow for validation (Overlay). x‐ and y ‐axes represent pixel co‐ordinates of the original images. Abbreviation: DAB, <t>3,3′‐diaminobenzidine</t>
    3 3 Diaminobenzidine Dab Substrate, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab substrate/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab substrate - by Bioz Stars, 2021-03
    97/100 stars
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    Image Search Results


    Image analysis workflow. Quantification of mesenchymal stromal cell/pericyte markers was performed using the CellProfiler image analysis software, and workflows were established for each respective antibody (representative CD146 and CD73 are shown). Fields of view were taken from the original image (Raw) and using the program threshold algorithms DAB stained regions (DAB regions) and nuclei (Nuclei) were defined (colored objects were arbitrarily assigned). Pixel area was determined from the colored segments detected in the DAB regions and nuclei. The images represent individual areas detected with either DAB or nuclei staining. These identified regions were subsequently overlaid on the original field of view to allow for validation (Overlay). x‐ and y ‐axes represent pixel co‐ordinates of the original images. Abbreviation: DAB, 3,3′‐diaminobenzidine

    Journal: Stem Cells Translational Medicine

    Article Title: Dynamic Changes in Brain Mesenchymal Perivascular Cells Associate with Multiple Sclerosis Disease Duration, Active Inflammation, and Demyelination

    doi: 10.1002/sctm.17-0028

    Figure Lengend Snippet: Image analysis workflow. Quantification of mesenchymal stromal cell/pericyte markers was performed using the CellProfiler image analysis software, and workflows were established for each respective antibody (representative CD146 and CD73 are shown). Fields of view were taken from the original image (Raw) and using the program threshold algorithms DAB stained regions (DAB regions) and nuclei (Nuclei) were defined (colored objects were arbitrarily assigned). Pixel area was determined from the colored segments detected in the DAB regions and nuclei. The images represent individual areas detected with either DAB or nuclei staining. These identified regions were subsequently overlaid on the original field of view to allow for validation (Overlay). x‐ and y ‐axes represent pixel co‐ordinates of the original images. Abbreviation: DAB, 3,3′‐diaminobenzidine

    Article Snippet: Bound antibody was detected using the 3,3′‐diaminobenzidine (DAB) substrate (DAKO) for HRP and the signal developed for an optimized time period.

    Techniques: Software, Staining

    Perinuclear localization of IDO2 protein in hepatocytes. IDO2 expression was analyzed by immunohistochemistry using a commercial mouse monoclonal antibody. Staining with the monoclonal antibody was observed around the nuclei in (A) Ido2 +/+ mice but absent in (B) Ido2 −/− mice. No staining was observed in tissue from Ido2 +/+ and Ido2 −/− mice with the isotype control antibody (C and D, respectively). IDO2 reactivity produced the brown staining from conversion of the diaminobenzidine substrate and the nuclei were stained blue with hematoxylin.

    Journal: International Journal of Tryptophan Research : IJTR

    Article Title: Investigation of the Tissue Distribution and Physiological Roles of Indoleamine 2,3-Dioxygenase-2

    doi: 10.1177/1178646917735098

    Figure Lengend Snippet: Perinuclear localization of IDO2 protein in hepatocytes. IDO2 expression was analyzed by immunohistochemistry using a commercial mouse monoclonal antibody. Staining with the monoclonal antibody was observed around the nuclei in (A) Ido2 +/+ mice but absent in (B) Ido2 −/− mice. No staining was observed in tissue from Ido2 +/+ and Ido2 −/− mice with the isotype control antibody (C and D, respectively). IDO2 reactivity produced the brown staining from conversion of the diaminobenzidine substrate and the nuclei were stained blue with hematoxylin.

    Article Snippet: Sections were washed thrice with agitation before diaminobenzidine substrate (Dako) was added and the sections incubated for 4 to 8 minutes (depending on the tissue type).

    Techniques: Expressing, Immunohistochemistry, Staining, Mouse Assay, Produced

    Tonsil sections stained with antibodies against (a) CD20 (diluted 1 : 1500), (b) CD3 (diluted 1 : 100), (c) Toll-like receptor (TLR)1 (diluted 1 : 50), (d) TLR2 (diluted 1 : 50), (e) TLR7 (diluted 1 : 50) and (f) TLR9 (diluted 1 : 50), were localized using 3,3′-diaminobenzidine (DAB), which stains tissues brown, and analysed by microscopy (magnification ×40–100).

    Journal: Immunology

    Article Title: A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by Pam3CSK4, R-837 and CpG-2006 stimulation

    doi: 10.1111/j.1365-2567.2006.02392.x

    Figure Lengend Snippet: Tonsil sections stained with antibodies against (a) CD20 (diluted 1 : 1500), (b) CD3 (diluted 1 : 100), (c) Toll-like receptor (TLR)1 (diluted 1 : 50), (d) TLR2 (diluted 1 : 50), (e) TLR7 (diluted 1 : 50) and (f) TLR9 (diluted 1 : 50), were localized using 3,3′-diaminobenzidine (DAB), which stains tissues brown, and analysed by microscopy (magnification ×40–100).

    Article Snippet: Subsequently, HRP-labelled goat anti-mouse or goat anti-rabbit polymer (Dako) was incubated with the sections for 30 min, followed by 3,3′-diaminobenzidine (DAB) substrate-chromogen (Dako) for 5–10 min. On some occasions, sections were counterstained with Mayer's haematoxylin.

    Techniques: Staining, Microscopy

    Histopathological analyzes of mice lung injected with immuno stimulants. After Lung tissues in (A) Carrageenan (B) Heat-killed yeast cells and (C) Zymozan injected mice. The presence of serine proteinase in the tissue was not detected. Scale bars are indicated. The polyclonal antibodies anti-serine-proteinase at 1:150 dilution was used. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin, and examined by light microscopy.

    Journal: Virulence

    Article Title: Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection

    doi: 10.1080/21505594.2017.1355660

    Figure Lengend Snippet: Histopathological analyzes of mice lung injected with immuno stimulants. After Lung tissues in (A) Carrageenan (B) Heat-killed yeast cells and (C) Zymozan injected mice. The presence of serine proteinase in the tissue was not detected. Scale bars are indicated. The polyclonal antibodies anti-serine-proteinase at 1:150 dilution was used. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin, and examined by light microscopy.

    Article Snippet: After, the slides were incubated with Mouse-on Mouse HRP-Polymer (Biocare Medical, #MM620G) for 30 min at 37°C for signal detection, followed by addition of Diaminobenzidine (DAB, Dako, #K3468-1).

    Techniques: Mouse Assay, Injection, Light Microscopy

    Histopathological images of lung sections at 6 h post-infection. (A) In situ presence of enolase, a control protein. (B) The presence of serine proteinase during infection in lung tissue. White arrows evidence fungal cells and black arrows evidence the labeling occurring also outside fungal cells. Scale bars are indicated. The polyclonal antibodies anti-enolase 25 at 1:150 dilution and anti-serine-proteinase 24 at 1:150 dilution, previously obtained, were used in A and B, respectively. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin and examined by light microscopy.

    Journal: Virulence

    Article Title: Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection

    doi: 10.1080/21505594.2017.1355660

    Figure Lengend Snippet: Histopathological images of lung sections at 6 h post-infection. (A) In situ presence of enolase, a control protein. (B) The presence of serine proteinase during infection in lung tissue. White arrows evidence fungal cells and black arrows evidence the labeling occurring also outside fungal cells. Scale bars are indicated. The polyclonal antibodies anti-enolase 25 at 1:150 dilution and anti-serine-proteinase 24 at 1:150 dilution, previously obtained, were used in A and B, respectively. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin and examined by light microscopy.

    Article Snippet: After, the slides were incubated with Mouse-on Mouse HRP-Polymer (Biocare Medical, #MM620G) for 30 min at 37°C for signal detection, followed by addition of Diaminobenzidine (DAB, Dako, #K3468-1).

    Techniques: Infection, In Situ, Labeling, Light Microscopy