diaminobenzidine tetrahydrochloride  (Millipore)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    3 3 Diaminobenzidine tetrahydrochloride
    Description:

    Catalog Number:
    d5905
    Price:
    None
    Applications:
    Reagent for spectrophotometric determination of selenium.
    Buy from Supplier


    Structured Review

    Millipore diaminobenzidine tetrahydrochloride
    3 3 Diaminobenzidine tetrahydrochloride

    https://www.bioz.com/result/diaminobenzidine tetrahydrochloride/product/Millipore
    Average 99 stars, based on 310 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine tetrahydrochloride - by Bioz Stars, 2021-02
    99/100 stars

    Images

    1) Product Images from "Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model"

    Article Title: Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

    Journal: Journal of Clinical Medicine

    doi: 10.3390/jcm10020272

    ( A ) Representative photomicrographs of the immunohistochemical reactions for leptin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for leptin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for ghrelin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for ghrelin in the jejunum, duodenum and duodenal Auerbach plexus (red arrow). Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ghrelin, measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for marvelD3. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of marvelD3, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); * p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for marvelD3. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of marvelD3, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); * p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for occludin. Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of occludin measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for occludin. Sections developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of occludin measured by the comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for nesfatin-1 in the jejunal crypts and ( C ) the Auerbach plexus (red arrow). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB); counterstaining was performed with Mayer’s hematoxylin. All the scale bars represent 50 μm. ( B ) The intensity of expression of nesfatin-1 in the jejunum, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for nesfatin-1 in the jejunal crypts and ( C ) the Auerbach plexus (red arrow). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB); counterstaining was performed with Mayer’s hematoxylin. All the scale bars represent 50 μm. ( B ) The intensity of expression of nesfatin-1 in the jejunum, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for Ki-67 in the jejunum. ( B ) Representative pictures of the immunohistochemical reactions for cadherin in the sections of the jejunum. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB); counterstaining was performed with Mayer’s hematoxylin. All the scale bars represent 50 μm. ( C ) The intensity of expression of cadherin in the jejunum, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. The scale was from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for Ki-67 in the jejunum. ( B ) Representative pictures of the immunohistochemical reactions for cadherin in the sections of the jejunum. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride (DAB); counterstaining was performed with Mayer’s hematoxylin. All the scale bars represent 50 μm. ( C ) The intensity of expression of cadherin in the jejunum, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. The scale was from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for vasoactive intestinal peptide (VIP) in Auerbach plexuses (red arrow) of the duodenum and the jejunum. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of VIP, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for vasoactive intestinal peptide (VIP) in Auerbach plexuses (red arrow) of the duodenum and the jejunum. Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of VIP, measured by the quantitative assessment of mean pixel intensity values in the photomicrographs converted to 8-bit grayscale images. Scale from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); ** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    ( A ) Representative photomicrographs of the immunohistochemical reactions for zonula occludens 1 (ZO-1). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ZO-1, measured by comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p
    Figure Legend Snippet: ( A ) Representative photomicrographs of the immunohistochemical reactions for zonula occludens 1 (ZO-1). Sections were developed in 3,3′-diaminobenzidine tetrahydrochloride with metal enhancer; counterstaining was performed with Nuclear Fast Red. All the scale bars represent 100 μm. ( B ) The intensity of expression of ZO-1, measured by comparison of the pixel brightness values in the microscopic images converted to 8-bit grayscale. The higher the pixel value, the lower the intensity of immunoreactions. Graph shows mean ± standard error. Significance was established using a two-tailed Student’s t -test (normally distributed data), Welch’s test (normally distributed data with unequal variances) or the Mann–Whitney test (for pairwise comparisons with at least one non-normally distributed dataset); *** p

    Techniques Used: Immunohistochemistry, Expressing, Two Tailed Test, MANN-WHITNEY

    2) Product Images from "Disinfection of Ocular Cells and Tissues by Atmospheric-Pressure Cold Plasma"

    Article Title: Disinfection of Ocular Cells and Tissues by Atmospheric-Pressure Cold Plasma

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0033245

    Tunel test on APCP treated corneal tissue. Apoptotic cells in 5 µm paraffin-embedded corneal tissues exposed for 2 minutes to APCP were identified by labelling DNA strand breaks with biotin-labeled deoxynucleotides and peroxidase. The immunoreaction product was visualized using 3,3′-diaminobenzidine and light microscopy. No significant apoptotic effects were evident in corneal tissues treated with APCP or only with helium (He). Data are expressed as the mean ± SE (error bars) of at least two independent experiments.
    Figure Legend Snippet: Tunel test on APCP treated corneal tissue. Apoptotic cells in 5 µm paraffin-embedded corneal tissues exposed for 2 minutes to APCP were identified by labelling DNA strand breaks with biotin-labeled deoxynucleotides and peroxidase. The immunoreaction product was visualized using 3,3′-diaminobenzidine and light microscopy. No significant apoptotic effects were evident in corneal tissues treated with APCP or only with helium (He). Data are expressed as the mean ± SE (error bars) of at least two independent experiments.

    Techniques Used: TUNEL Assay, Labeling, Light Microscopy

    3) Product Images from "Upregulation of activin signaling in experimental colitis"

    Article Title: Upregulation of activin signaling in experimental colitis

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    doi: 10.1152/ajpgi.90631.2008

    Expression of activins in normal and colitic colon. Sections of distal part of the colon were stained with βA-antibodies ( A and B ) or βB-antibodies ( C – F ), detected by 3,3′- diaminobenzidine tetrahydrochloride (DAB), and
    Figure Legend Snippet: Expression of activins in normal and colitic colon. Sections of distal part of the colon were stained with βA-antibodies ( A and B ) or βB-antibodies ( C – F ), detected by 3,3′- diaminobenzidine tetrahydrochloride (DAB), and

    Techniques Used: Expressing, Staining

    4) Product Images from "Prevalence of small testicular hyperechogenic foci in subgroups of 382 non‐vasectomized, azoospermic men: a retrospective cohort study"

    Article Title: Prevalence of small testicular hyperechogenic foci in subgroups of 382 non‐vasectomized, azoospermic men: a retrospective cohort study

    Journal: Andrology

    doi: 10.1111/andr.12291

    Cytokeratin‐18 ( CK ‐18), a marker for Sertoli cell immaturity, detected in the seminiferous tubules using a three‐stage immunoperoxidase technique as described in detail in the main text. Presence of CK ‐18 was visualized using diaminobenzidine tetrahydrochloride ( DAB ). (A) A 29‐year‐old man treated twice with chemotherapy because of Hodgkin's disease, with Sertoli cells in a biopsy, positive for CK ‐18. (B) A 36‐year‐old proven fertile man undergoing vasectomy, negative for CK ‐18.
    Figure Legend Snippet: Cytokeratin‐18 ( CK ‐18), a marker for Sertoli cell immaturity, detected in the seminiferous tubules using a three‐stage immunoperoxidase technique as described in detail in the main text. Presence of CK ‐18 was visualized using diaminobenzidine tetrahydrochloride ( DAB ). (A) A 29‐year‐old man treated twice with chemotherapy because of Hodgkin's disease, with Sertoli cells in a biopsy, positive for CK ‐18. (B) A 36‐year‐old proven fertile man undergoing vasectomy, negative for CK ‐18.

    Techniques Used: Marker

    5) Product Images from "CCR5 Plays a Critical Role in the Development of Myocarditis and Host Protection in Mice Infected with Trypanosoma cruzi"

    Article Title: CCR5 Plays a Critical Role in the Development of Myocarditis and Host Protection in Mice Infected with Trypanosoma cruzi

    Journal: The Journal of Infectious Diseases

    doi: 10.1086/427515

    Expression of CCL3, CCL4, and CCL5 in the myocardia of Trypanosoma cruzi -infected mice. A , Total myocardial RNA was extracted from uninfected (U) and infected (I) mice on day 15 after infection, and expression of CCL3, CCL4, CCL5, and β-actin was assessed by reverse-transcription polymerase chain reaction (PCR). The PCR products were electrophoresed in polyacrylamide gels and stained with silver nitrate. B , Hearts of uninfected ( a, c , and e ) and infected ( b, d , and f ) mice on day 20 after infection with 1000 trypomastigote forms of T. cruzi were prepared for immunohistochemical analysis, and the presence of CCL4 ( a and b ), CCL3 ( c and d ), and CCL5 ( e and f ) were evaluated by use of the immunoperoxidase method (see Materials and Methods). The presence of the chemokines was revealed by use of diaminobenzidine tetrahydrochloride as the substratum for the peroxidase, which generated a brown coloration. Results shown are representative of 3 different experiments. Original magnification for all microphotographs, x 200.
    Figure Legend Snippet: Expression of CCL3, CCL4, and CCL5 in the myocardia of Trypanosoma cruzi -infected mice. A , Total myocardial RNA was extracted from uninfected (U) and infected (I) mice on day 15 after infection, and expression of CCL3, CCL4, CCL5, and β-actin was assessed by reverse-transcription polymerase chain reaction (PCR). The PCR products were electrophoresed in polyacrylamide gels and stained with silver nitrate. B , Hearts of uninfected ( a, c , and e ) and infected ( b, d , and f ) mice on day 20 after infection with 1000 trypomastigote forms of T. cruzi were prepared for immunohistochemical analysis, and the presence of CCL4 ( a and b ), CCL3 ( c and d ), and CCL5 ( e and f ) were evaluated by use of the immunoperoxidase method (see Materials and Methods). The presence of the chemokines was revealed by use of diaminobenzidine tetrahydrochloride as the substratum for the peroxidase, which generated a brown coloration. Results shown are representative of 3 different experiments. Original magnification for all microphotographs, x 200.

    Techniques Used: Expressing, Infection, Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Staining, Immunohistochemistry, Generated

    Expression of CCR5, CD8, and CD4 in the myocardia of uninfected or Trypanosoma cruzi -infected mice. Hearts of uninfected ( A ) or infected ( B-F ) mice on day 17 after infection with 1000 trypomastigote forms of T. cruzi were obtained and prepared for analysis by immunohistochemistry and immunofluorescence. Expression of CCR5 ( A and B ), CD4 ( C ), and CD8 ( D ) in myocardia was evaluated by use of the immunoperoxidase method (see Materials and Methods). Diaminobenzidine tetrahydrochloride was used as the substratum for the peroxidase, which generated a brown coloration. Panels E and F show the results of double immunofluorescence labeling, in which CD4 ( E ) and CD8 ( F ) were stained with fluorescein-labeled specific antibody and CCR5 ( E and F ) was stained with antibody conjugated with Texas red. Original magnification for all microphotographs, x 200.
    Figure Legend Snippet: Expression of CCR5, CD8, and CD4 in the myocardia of uninfected or Trypanosoma cruzi -infected mice. Hearts of uninfected ( A ) or infected ( B-F ) mice on day 17 after infection with 1000 trypomastigote forms of T. cruzi were obtained and prepared for analysis by immunohistochemistry and immunofluorescence. Expression of CCR5 ( A and B ), CD4 ( C ), and CD8 ( D ) in myocardia was evaluated by use of the immunoperoxidase method (see Materials and Methods). Diaminobenzidine tetrahydrochloride was used as the substratum for the peroxidase, which generated a brown coloration. Panels E and F show the results of double immunofluorescence labeling, in which CD4 ( E ) and CD8 ( F ) were stained with fluorescein-labeled specific antibody and CCR5 ( E and F ) was stained with antibody conjugated with Texas red. Original magnification for all microphotographs, x 200.

    Techniques Used: Expressing, Infection, Mouse Assay, Immunohistochemistry, Immunofluorescence, Generated, Labeling, Staining

    Related Articles

    Staining:

    Article Title: Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen
    Article Snippet: .. The HRP complex was visualized by staining with a substrate solution containing 3.3′‐diaminobenzidine tetrahydrochloride (Sigma). ..

    Incubation:

    Article Title: Genetic and Molecular Characterization of Submergence Response Identifies Subtol6 as a Major Submergence Tolerance Locus in Maize
    Article Snippet: .. For H2 O2 visualization, leaf tissue was collected as described above, immersed in 1 mg/mL 3,3-diaminobenzidine tetrahydrochloride (DAB; Sigma-Aldrich, St. Louis, MO, USA) in 50 mM tris-acetate buffer (pH 5.0), and were incubated at 25ºC for 24 h in complete darkness. ..

    Article Title: Fos Expression in Neurons of the Rat Vestibulo-Autonomic Pathway Activated by Sinusoidal Galvanic Vestibular Stimulation
    Article Snippet: .. Sections were then rinsed in PBS (six changes over 2 h), and incubated in Tris buffer (pH 7.6) containing 0.5 mg/ml diaminobenzidine (DAB; Sigma D-5905; St. Louis, MO, USA) with 0.01% H2 O2 . .. Microscopy, stain analysis, and image preparation Sections were examined and images were acquired with a Zeiss Axioplan2 microscope equipped for structured illumination (ApoTome).

    other:

    Article Title: A Pyrimidin-Like Plant Activator Stimulates Plant Disease Resistance and Promotes the Synthesis of Primary Metabolites
    Article Snippet: Trypan blue and diaminobenzidine tetrahydrochloride (DAB) were purchased from Sigma.

    Mouse Assay:

    Article Title: GABA–glutamate supramammillary neurons control theta and gamma oscillations in the dentate gyrus during paradoxical (REM) sleep
    Article Snippet: .. Sections from VGLUT2-EYFP and VGLUT2-ChR2 mice were processed in parallel and for the same period of time (15 min) in 3.3′-diaminobenzidine tetrahydrochloride (DAB, Sigma fast tablets; Sigma), rinsed in KPBS, mounted onto Superfrost Plus slides, dehydrated and coverslipped with Permount. .. Quantification of cFos immunolabeled neurons The number of cFos labeled neurons was calculated in the DG GCL of the right and left (ipsilateral to the optic stimulation) hemispheres in VGLUT2-EYFP control (n = 4), and VGLUT2-ChR2 (n = 4) mice.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore dab solution
    <t>NF200</t> <t>DAB</t> staining results ( a , b ) and 5HT immunohistochemical results ( c , d ) in the injured spinal cord tissue at 12 weeks after injury ( a – d ). ( a ) Representative images of NF200-positive axons in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 1–4) within the lesion cavities of contused spinal cords (outlined by black dashed lines). ( b ) The density of NF200-positive axons of the control and exercise groups ( n = 4 per group). ( c ) Representative images of 5HT-positive axons (green) and GFAP-positive astrocytes (red) in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 5–8) adjacent to the lesion cavities of contused spinal cords. ( d ) The density of 5HT-positive axons of the control and exercise groups ( n = 4 per group). * p
    Dab Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dab solution/product/Millipore
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    dab solution - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    95
    Millipore 3 3 diaminobenzidine dab enhanced liquid substrate system tetrahydrochloride
    Primary roots of sorghum seedlings stained with <t>3,3’-diaminobenzidine</t> <t>(DAB).</t> Cross sections taken from a Si− (a-c) and a Si+ root (d-f) represent regions IV, III, and II of silica aggregation along the root, as defined in Figure 1 . Insets are enlargements of the red rectangles in each of the panels. Brown discoloration attributed to the precipitation of DAB can be seen in the endodermal ITCW and the cell walls of xylem elements in all three regions. The staining is more intense in region II of both treatments. This is the region of Si aggregation onset. Arrowheads indicate some silica aggregates. (g) A longitudinal view of DAB stained ITCW from a Si− root. Slightly stronger tainted spots (arrows) can be seen on the background of the stained cell wall. Pits are seen as dark dots. (h) A similar longitudinal view of a Si+ ITCW. The outlines of Si aggregates are evident in darker stain (arrows) on the uniformly stained ITCW. Scale bar in f, common to a-f represents 50 μm. Scale bar in h, common to g and h represents 5 μm.
    3 3 Diaminobenzidine Dab Enhanced Liquid Substrate System Tetrahydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab enhanced liquid substrate system tetrahydrochloride/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab enhanced liquid substrate system tetrahydrochloride - by Bioz Stars, 2021-02
    95/100 stars
      Buy from Supplier

    99
    Millipore diaminobenzidine dab stainings
    Primary roots of sorghum seedlings stained with <t>3,3’-diaminobenzidine</t> <t>(DAB).</t> Cross sections taken from a Si− (a-c) and a Si+ root (d-f) represent regions IV, III, and II of silica aggregation along the root, as defined in Figure 1 . Insets are enlargements of the red rectangles in each of the panels. Brown discoloration attributed to the precipitation of DAB can be seen in the endodermal ITCW and the cell walls of xylem elements in all three regions. The staining is more intense in region II of both treatments. This is the region of Si aggregation onset. Arrowheads indicate some silica aggregates. (g) A longitudinal view of DAB stained ITCW from a Si− root. Slightly stronger tainted spots (arrows) can be seen on the background of the stained cell wall. Pits are seen as dark dots. (h) A similar longitudinal view of a Si+ ITCW. The outlines of Si aggregates are evident in darker stain (arrows) on the uniformly stained ITCW. Scale bar in f, common to a-f represents 50 μm. Scale bar in h, common to g and h represents 5 μm.
    Diaminobenzidine Dab Stainings, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diaminobenzidine dab stainings/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine dab stainings - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier


    Image Search Results


    NF200 DAB staining results ( a , b ) and 5HT immunohistochemical results ( c , d ) in the injured spinal cord tissue at 12 weeks after injury ( a – d ). ( a ) Representative images of NF200-positive axons in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 1–4) within the lesion cavities of contused spinal cords (outlined by black dashed lines). ( b ) The density of NF200-positive axons of the control and exercise groups ( n = 4 per group). ( c ) Representative images of 5HT-positive axons (green) and GFAP-positive astrocytes (red) in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 5–8) adjacent to the lesion cavities of contused spinal cords. ( d ) The density of 5HT-positive axons of the control and exercise groups ( n = 4 per group). * p

    Journal: Cells

    Article Title: Exercise Ameliorates Spinal Cord Injury by Changing DNA Methylation

    doi: 10.3390/cells10010143

    Figure Lengend Snippet: NF200 DAB staining results ( a , b ) and 5HT immunohistochemical results ( c , d ) in the injured spinal cord tissue at 12 weeks after injury ( a – d ). ( a ) Representative images of NF200-positive axons in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 1–4) within the lesion cavities of contused spinal cords (outlined by black dashed lines). ( b ) The density of NF200-positive axons of the control and exercise groups ( n = 4 per group). ( c ) Representative images of 5HT-positive axons (green) and GFAP-positive astrocytes (red) in the control and exercise groups. The right images are high-magnification images of the black boxes in the left images (Nos. 5–8) adjacent to the lesion cavities of contused spinal cords. ( d ) The density of 5HT-positive axons of the control and exercise groups ( n = 4 per group). * p

    Article Snippet: Finally, NF200 staining was revealed with DAB solution (0.05% 3–3-diaminobenzidine tetrahydrochloride (MilliporeSigma), 0.06% NiCl2 (MilliporeSigma), and 0.003% H2 O2), and the reaction was stopped with distilled water.

    Techniques: Staining, Immunohistochemistry

    Primary roots of sorghum seedlings stained with 3,3’-diaminobenzidine (DAB). Cross sections taken from a Si− (a-c) and a Si+ root (d-f) represent regions IV, III, and II of silica aggregation along the root, as defined in Figure 1 . Insets are enlargements of the red rectangles in each of the panels. Brown discoloration attributed to the precipitation of DAB can be seen in the endodermal ITCW and the cell walls of xylem elements in all three regions. The staining is more intense in region II of both treatments. This is the region of Si aggregation onset. Arrowheads indicate some silica aggregates. (g) A longitudinal view of DAB stained ITCW from a Si− root. Slightly stronger tainted spots (arrows) can be seen on the background of the stained cell wall. Pits are seen as dark dots. (h) A similar longitudinal view of a Si+ ITCW. The outlines of Si aggregates are evident in darker stain (arrows) on the uniformly stained ITCW. Scale bar in f, common to a-f represents 50 μm. Scale bar in h, common to g and h represents 5 μm.

    Journal: bioRxiv

    Article Title: Hydrogen peroxide modulates lignin and silica deposits in sorghum roots

    doi: 10.1101/2021.02.01.429181

    Figure Lengend Snippet: Primary roots of sorghum seedlings stained with 3,3’-diaminobenzidine (DAB). Cross sections taken from a Si− (a-c) and a Si+ root (d-f) represent regions IV, III, and II of silica aggregation along the root, as defined in Figure 1 . Insets are enlargements of the red rectangles in each of the panels. Brown discoloration attributed to the precipitation of DAB can be seen in the endodermal ITCW and the cell walls of xylem elements in all three regions. The staining is more intense in region II of both treatments. This is the region of Si aggregation onset. Arrowheads indicate some silica aggregates. (g) A longitudinal view of DAB stained ITCW from a Si− root. Slightly stronger tainted spots (arrows) can be seen on the background of the stained cell wall. Pits are seen as dark dots. (h) A similar longitudinal view of a Si+ ITCW. The outlines of Si aggregates are evident in darker stain (arrows) on the uniformly stained ITCW. Scale bar in f, common to a-f represents 50 μm. Scale bar in h, common to g and h represents 5 μm.

    Article Snippet: 3,3’-diaminobenzidine (DAB) staining and roots sectioningDAB staining was preformed according to ( ) with minor changes.

    Techniques: Staining