dh5α (Toyobo)
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Dh5α, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dh5α/product/Toyobo
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease"
Article Title: An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease
Journal: The EMBO Journal
doi: 10.1038/sj.emboj.7600049

Figure Legend Snippet: Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into DH5α. ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.
Techniques Used: Selection, Isolation, TUNEL Assay, Staining, Activation Assay, Activity Assay
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