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Toyobo dh5α
Dh5α, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dh5α/product/Toyobo
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dh5α - by Bioz Stars, 2020-01
92/100 stars

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DNA Extraction:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: DNA was isolated from these cells using Genelute mammalian genomic DNA extraction kit, and digested by restriction enzyme BglII (Takara Bio) and BamHI (Toyobo). .. The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit.

Transfection:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: Two days after transfection, cells were harvested, transferred to 100 mm plates and propagated in medium containing 3 µg/ml blasticidin S (InvivoGen). .. The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit.

Luciferase:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit. .. Assay of firefly luciferase activity in liver.

Ligation:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: .. The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit. .. Nucleotide sequences of the pDNA were sequenced by Fasmac sequencing service (Fasmac, Atsugi, Japan).

Isolation:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: .. The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit. .. Nucleotide sequences of the pDNA were sequenced by Fasmac sequencing service (Fasmac, Atsugi, Japan).

Purification:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: .. The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit. .. Nucleotide sequences of the pDNA were sequenced by Fasmac sequencing service (Fasmac, Atsugi, Japan).

Polymerase Chain Reaction:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: After digestion by restriction enzymes, DNA was purified using High Pure PCR Product Purification Kit and ligated using Ligation-Convenience Kit (Nippon Gene). .. The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit.

Activity Assay:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit. .. Assay of firefly luciferase activity in liver.

Sequencing:

Article Title: piggyBac Transposon-mediated Long-term Gene Expression in Mice
Article Snippet: The ligation products were used to transform E. coli Competent Quick DH5α (Toyobo) or E. coli HST08 Premium Competent Cells (Takara Bio). pDNA was isolated and purified using QIAprep Spin Miniprep Kit. .. Nucleotide sequences of the pDNA were sequenced by Fasmac sequencing service (Fasmac, Atsugi, Japan).

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  • 78
    Toyobo coli dh5α strain
    Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli <t>DH5α</t> (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P
    Coli Dh5α Strain, supplied by Toyobo, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli dh5α strain/product/Toyobo
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    coli dh5α strain - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    92
    Toyobo dh5α
    Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into <t>DH5α.</t> ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.
    Dh5α, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dh5α/product/Toyobo
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dh5α - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    78
    Toyobo bacterial strains dh5α chemical competent e coli
    Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into <t>DH5α.</t> ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.
    Bacterial Strains Dh5α Chemical Competent E Coli, supplied by Toyobo, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial strains dh5α chemical competent e coli/product/Toyobo
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacterial strains dh5α chemical competent e coli - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    Image Search Results


    Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Mutagenesis, Negative Control

    Bacterial adherence to Caco-2 cells for the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), PAO1Tn:: flgE mutant ( ΔflgE ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (20, 30, 40, and 50 mM). Bacterial adherence was determined based on the number of adhered bacteria per Caco-2 cell. The assay was performed in six replicates, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: Bacterial adherence to Caco-2 cells for the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), PAO1Tn:: flgE mutant ( ΔflgE ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (20, 30, 40, and 50 mM). Bacterial adherence was determined based on the number of adhered bacteria per Caco-2 cell. The assay was performed in six replicates, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Mutagenesis, Negative Control

    Penetration activity of P . aeruginosa strains. (A) The wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), and PAO1Tn:: flgE mutant ( ΔflgE ) were inoculated onto the apical surfaces of Caco-2 cell monolayers at an MOI of 100, and the number of bacteria in the basolateral medium was counted at 6 h after infection. The assay was performed in triplicate, and the results are expressed as the mean ± SD. E . coli DH5α was used as a negative control. *#: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: Penetration activity of P . aeruginosa strains. (A) The wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), and PAO1Tn:: flgE mutant ( ΔflgE ) were inoculated onto the apical surfaces of Caco-2 cell monolayers at an MOI of 100, and the number of bacteria in the basolateral medium was counted at 6 h after infection. The assay was performed in triplicate, and the results are expressed as the mean ± SD. E . coli DH5α was used as a negative control. *#: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Activity Assay, Mutagenesis, Infection, Negative Control

    PGDH activity assay. (A) SDS-PAGE analysis to verify overexpression of the P . aeruginosa serA and synthesis of SerA proteins in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The red arrow indicates an overexpressed band at the expected size of 44 kDa for P . aeruginosa SerA. (B) Inhibitory effect of 50 mM L -serine on PGDH activity of SerA proteins (mU/mg) in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The assay was performed in five replicates, and the results are expressed as the mean ± SD. Significant differences were observed between SerA and mock (*: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: PGDH activity assay. (A) SDS-PAGE analysis to verify overexpression of the P . aeruginosa serA and synthesis of SerA proteins in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The red arrow indicates an overexpressed band at the expected size of 44 kDa for P . aeruginosa SerA. (B) Inhibitory effect of 50 mM L -serine on PGDH activity of SerA proteins (mU/mg) in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The assay was performed in five replicates, and the results are expressed as the mean ± SD. Significant differences were observed between SerA and mock (*: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Activity Assay, SDS Page, Over Expression, Isolation

    Motility assays. (A) Swimming motility of WT, ΔserA , + serA , and E . coli DH5α (as a negative control). A representative image from the swimming motility assay is shown. The major axis of swimming is the longest length of the swimming area. The assay was performed in triplicate, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: Motility assays. (A) Swimming motility of WT, ΔserA , + serA , and E . coli DH5α (as a negative control). A representative image from the swimming motility assay is shown. The major axis of swimming is the longest length of the swimming area. The assay was performed in triplicate, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Negative Control, Motility Assay

    Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into DH5α. ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.

    Journal: The EMBO Journal

    Article Title: An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease

    doi: 10.1038/sj.emboj.7600049

    Figure Lengend Snippet: Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into DH5α. ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.

    Article Snippet: For the first screening, transfected cells were exposed to Tm for 24 h. For the second screening, cells were transfected with candidate plasmids from the first screening and collected after incubation with Tm for 48 h. Expression vectors harboring Rzs were isolated from surviving cells and used to transform DH5α (TOYOBO, Tokyo, Japan).

    Techniques: Selection, Isolation, TUNEL Assay, Staining, Activation Assay, Activity Assay