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Polyamine metabolism. Putrescine (Put) is synthesized from ornithine by ornithine decarboxylase (ODC) and then converted to spermidine (Spd) and spermine (Spm) by spermidine synthase (SpdSyn) and spermine synthase (SpmSyn), respectively. The aminopropyl group required for the synthesis of Spd and Spm is derived from decarboxylated S -adenosylmethionine (dcSAM). <t>DFMO</t> is an inhibitor of <t>ODC.</t> <t>APCHA</t> is an inhibitor of SpmSyn.
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Polyamine metabolism. Putrescine (Put) is synthesized from ornithine by ornithine decarboxylase (ODC) and then converted to spermidine (Spd) and spermine (Spm) by spermidine synthase (SpdSyn) and spermine synthase (SpmSyn), respectively. The aminopropyl group required for the synthesis of Spd and Spm is derived from decarboxylated S -adenosylmethionine (dcSAM). <t>DFMO</t> is an inhibitor of <t>ODC.</t> <t>APCHA</t> is an inhibitor of SpmSyn.
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Polyamine metabolism. Putrescine (Put) is synthesized from ornithine by ornithine decarboxylase (ODC) and then converted to spermidine (Spd) and spermine (Spm) by spermidine synthase (SpdSyn) and spermine synthase (SpmSyn), respectively. The aminopropyl group required for the synthesis of Spd and Spm is derived from decarboxylated S -adenosylmethionine (dcSAM). <t>DFMO</t> is an inhibitor of <t>ODC.</t> <t>APCHA</t> is an inhibitor of SpmSyn.
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Polyamine metabolism. Putrescine (Put) is synthesized from ornithine by ornithine decarboxylase (ODC) and then converted to spermidine (Spd) and spermine (Spm) by spermidine synthase (SpdSyn) and spermine synthase (SpmSyn), respectively. The aminopropyl group required for the synthesis of Spd and Spm is derived from decarboxylated S -adenosylmethionine (dcSAM). DFMO is an inhibitor of ODC. APCHA is an inhibitor of SpmSyn.

Journal: The Journal of Biological Chemistry

Article Title: Polyamines enhance repeat-associated non-AUG translation from CCUG repeats by stabilizing the tertiary structure of RNA

doi: 10.1016/j.jbc.2025.108251

Figure Lengend Snippet: Polyamine metabolism. Putrescine (Put) is synthesized from ornithine by ornithine decarboxylase (ODC) and then converted to spermidine (Spd) and spermine (Spm) by spermidine synthase (SpdSyn) and spermine synthase (SpmSyn), respectively. The aminopropyl group required for the synthesis of Spd and Spm is derived from decarboxylated S -adenosylmethionine (dcSAM). DFMO is an inhibitor of ODC. APCHA is an inhibitor of SpmSyn.

Article Snippet: After 14 h of incubation, the media was changed to fresh media supplemented with aminoguanidine (1 mM, Wako) for all samples and with DFMO (0.5 mM) plus APCHA (0.2 mM) , Put (100 μM, Wako), or Spm (50 μM, Nacalai) as appropriate (presented as final concentration in medium).

Techniques: Synthesized, Derivative Assay