dextran sulfate sodium dss  (Valiant)

 
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    Name:
    Dextran
    Description:
    Dextran
    Catalog Number:
    0520519580
    Price:
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    Applications:
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    Size:
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    Structured Review

    Valiant dextran sulfate sodium dss
    Dextran
    Dextran
    https://www.bioz.com/result/dextran sulfate sodium dss/product/Valiant
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dextran sulfate sodium dss - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice"

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice

    Journal: Gastroenterology

    doi: 10.1053/j.gastro.2010.10.061

    Klf5 +/− mice show impaired recovery during the DSS recovery phase. WT and Klf5 +/− mice were treated with DSS (3.5% and 2.5%, respectively) for 7 days to achieve similar degrees of colitis prior to 5 day recovery phase. (A) Weights of WT and Klf5 +/− mice after 7 days of DSS treatment and after 5 days of recovery (n = 14, * P = 0.02.) (B) Kaplan-Meier survival curve of WT and Klf5 +/− mice during DSS treatment and recovery phase (n = 14; P = 0.02). (C D) Clinical and histological scores after 7 days of DSS treatment or after 5 days of recovery. (C) n = 7; * P = 0.003; ** P = 0.05 (D) n = 7; * P
    Figure Legend Snippet: Klf5 +/− mice show impaired recovery during the DSS recovery phase. WT and Klf5 +/− mice were treated with DSS (3.5% and 2.5%, respectively) for 7 days to achieve similar degrees of colitis prior to 5 day recovery phase. (A) Weights of WT and Klf5 +/− mice after 7 days of DSS treatment and after 5 days of recovery (n = 14, * P = 0.02.) (B) Kaplan-Meier survival curve of WT and Klf5 +/− mice during DSS treatment and recovery phase (n = 14; P = 0.02). (C D) Clinical and histological scores after 7 days of DSS treatment or after 5 days of recovery. (C) n = 7; * P = 0.003; ** P = 0.05 (D) n = 7; * P

    Techniques Used: Mouse Assay

    EGFR levels are reduced at sites of ulceration in Klf5 +/− mice treated with DSS. (A) EGFR staining in colons of mice after 5 days of DSS treatment (acute phase); panel 1, WT; panel 2, Klf5 +/− . EGFR staining after 5 days of recovery from DSS treatment. Panel 3, WT (enlargement, panel 5); panel 4, Klf5 +/− (enlargement, panel 6) Red arrows indicate luminal epithelial cells. (B) Western blots of colon lysates from WT and Klf5 +/− mice treated with 3.5% DSS, days 0–5. Lysates are pooled from 3 separate mice. (C) Effects of Klf5 expression on EGFR levels in colon cancer cells. Western blot analysis with inhibition of KLF5 by siRNA in DLD-1 cells or overexpression of KLF5 in HCT 116 cells.
    Figure Legend Snippet: EGFR levels are reduced at sites of ulceration in Klf5 +/− mice treated with DSS. (A) EGFR staining in colons of mice after 5 days of DSS treatment (acute phase); panel 1, WT; panel 2, Klf5 +/− . EGFR staining after 5 days of recovery from DSS treatment. Panel 3, WT (enlargement, panel 5); panel 4, Klf5 +/− (enlargement, panel 6) Red arrows indicate luminal epithelial cells. (B) Western blots of colon lysates from WT and Klf5 +/− mice treated with 3.5% DSS, days 0–5. Lysates are pooled from 3 separate mice. (C) Effects of Klf5 expression on EGFR levels in colon cancer cells. Western blot analysis with inhibition of KLF5 by siRNA in DLD-1 cells or overexpression of KLF5 in HCT 116 cells.

    Techniques Used: Mouse Assay, Staining, Western Blot, Expressing, Inhibition, Over Expression

    Induction of KLF5 with DSS treatment is dependent on ERK1/2 signaling. (A) Detection of ERK1/2 and NF-κB activation by Western blotting, using pooled lysates from WT mice treated with 3.5% DSS over a 5 day time course (n=3). (B) Caco-2 cells were treated with 3% DSS for up to 8 hrs, and lysates were examined by Western blotting. (C) Caco-2 cells were pretreated with inhibitors of MEK/ERK (U0126) or NF-κB (Bay 11-7082), and treated for 5 hrs with 3% DSS. Lysates were examined by Western blotting.
    Figure Legend Snippet: Induction of KLF5 with DSS treatment is dependent on ERK1/2 signaling. (A) Detection of ERK1/2 and NF-κB activation by Western blotting, using pooled lysates from WT mice treated with 3.5% DSS over a 5 day time course (n=3). (B) Caco-2 cells were treated with 3% DSS for up to 8 hrs, and lysates were examined by Western blotting. (C) Caco-2 cells were pretreated with inhibitors of MEK/ERK (U0126) or NF-κB (Bay 11-7082), and treated for 5 hrs with 3% DSS. Lysates were examined by Western blotting.

    Techniques Used: Activation Assay, Western Blot, Mouse Assay

    Klf5 expression is reduced at sites of ulceration in DSS-treated Klf5 +/− mice. (A) Immunofluorescence staining of Klf5 in WT and Klf5 +/− mice untreated or treated with DSS for 7 days. White arrows indicate Klf5 staining at luminal surface (B) Quantification of fluorescent intensities of Klf5 staining per cell. Data represent the mean ± SEM of 5 mice per group with at least 50 cells quantified per sample. * P = 0.01; ** P = 0.04. (C) Quantification of Klf5-positive cells per crypt in untreated and DSS-treated WT and Klf5 +/− mice. Data represent the mean ± SEM of 3 mice per group, counting 5 crypts per mouse. * P
    Figure Legend Snippet: Klf5 expression is reduced at sites of ulceration in DSS-treated Klf5 +/− mice. (A) Immunofluorescence staining of Klf5 in WT and Klf5 +/− mice untreated or treated with DSS for 7 days. White arrows indicate Klf5 staining at luminal surface (B) Quantification of fluorescent intensities of Klf5 staining per cell. Data represent the mean ± SEM of 5 mice per group with at least 50 cells quantified per sample. * P = 0.01; ** P = 0.04. (C) Quantification of Klf5-positive cells per crypt in untreated and DSS-treated WT and Klf5 +/− mice. Data represent the mean ± SEM of 3 mice per group, counting 5 crypts per mouse. * P

    Techniques Used: Expressing, Mouse Assay, Immunofluorescence, Staining

    Klf5 +/− mice exhibit increased susceptibility to colitis with DSS treatment. Eight-week old WT and Klf5 +/− mice were treated with 3.5% DSS for 7 days and examined for clinical signs of colitis. Colon tissues were processed for enzymatic and histological analysis. (A) Weights of mice during 7 day treatment period; n = 9 mice per group, a : P
    Figure Legend Snippet: Klf5 +/− mice exhibit increased susceptibility to colitis with DSS treatment. Eight-week old WT and Klf5 +/− mice were treated with 3.5% DSS for 7 days and examined for clinical signs of colitis. Colon tissues were processed for enzymatic and histological analysis. (A) Weights of mice during 7 day treatment period; n = 9 mice per group, a : P

    Techniques Used: Mouse Assay

    Klf5 is induced in wild-type (WT) mice treated with DSS. Eight week-old WT mice were given water or treated with 3.5% DSS (wt/vol). (A) Immunohistochemical staining of colon of Klf5 in WT mice untreated or treated with DSS for 7 days (brown, Klf5; blue; counterstain). Red bars and arrows indicate regions of Klf5 expression. (B) Western blots of lysates from WT mice treated with DSS over a 5 day time course. Lysates for each time point were pooled from 3 separate mice.
    Figure Legend Snippet: Klf5 is induced in wild-type (WT) mice treated with DSS. Eight week-old WT mice were given water or treated with 3.5% DSS (wt/vol). (A) Immunohistochemical staining of colon of Klf5 in WT mice untreated or treated with DSS for 7 days (brown, Klf5; blue; counterstain). Red bars and arrows indicate regions of Klf5 expression. (B) Western blots of lysates from WT mice treated with DSS over a 5 day time course. Lysates for each time point were pooled from 3 separate mice.

    Techniques Used: Mouse Assay, Immunohistochemistry, Staining, Expressing, Western Blot

    2) Product Images from "Goblet cell LRRC26 regulates BK channel activation and protects against colitis in mice"

    Article Title: Goblet cell LRRC26 regulates BK channel activation and protects against colitis in mice

    Journal: bioRxiv

    doi: 10.1101/2020.10.23.341396

    Genetic ablation of BKα subunit dramatically enhances susceptibility to DSS-induced colitis. Survival plots ( A ) and change in body weight ( B ) curves of mice treated for 7 days with 2.5% DSS (MP Biomedicals, MW: 36-50 KDa). Animals whose body weight drop > 30% of their initial weight were considered terminal and euthanized. Graphs compile results from 4 independent assays where 6 litters (with both BK -/- and WT littermates) Mice were 10-14 weeks old at the time of the treatment and both sexes were included. Change in body weight plot represents means±SEM. Curves were compared using a two-way ANOVA that yielded P
    Figure Legend Snippet: Genetic ablation of BKα subunit dramatically enhances susceptibility to DSS-induced colitis. Survival plots ( A ) and change in body weight ( B ) curves of mice treated for 7 days with 2.5% DSS (MP Biomedicals, MW: 36-50 KDa). Animals whose body weight drop > 30% of their initial weight were considered terminal and euthanized. Graphs compile results from 4 independent assays where 6 litters (with both BK -/- and WT littermates) Mice were 10-14 weeks old at the time of the treatment and both sexes were included. Change in body weight plot represents means±SEM. Curves were compared using a two-way ANOVA that yielded P

    Techniques Used: Mouse Assay

    Genetic ablation of LRRC26 dramatically enhances susceptibility to DSS-induced colitis. A-D , Survival plots and change in body weight curves of mice treated for 7 days with 2.5% ( A-B ) or 3.5% DSS (MP Biomedicals, MW: 36-50 KDa) ( C-D ). In accordance with guidance from WUSTL-animal studies committee, animals whose body weight dropped > 30% of their initial value were considered terminal and euthanized. Graphs compile results from 4 independent assays where 8 litters (with both Lrrc26 -/- and WT littermates) were treated with 2.5% DSS or 2 assays where 4 litters were treated with 3.5%. Mice were 10-14 weeks old at the time of the treatment and both sexes were included. In body weight plots (mean ± SEM), for mice that died or required euthanasia, weight at the time of death was considered the same until the end of the experiment. Curves were compared using a two-way ANOVA that yielded P=0.0117 for 2.5% and P=0.0073 for 3.5% DSS. E , Representative images of H E stained most distal segment of colon from Lrrc26 - and WT (littermates) at the 7 th day of treatment (bars: 2.5 mm). F , Areas within the squares in (E), (bars 250 μm). G-I , Histophatologic colitis severity in the distal colon of DSS-treated mice scored from sections as in E-F. Inflammation score ( G ), total ulcer extention ( H ) and fraction of epithelial surface with 50% or more of crypt loss ( I ), represented as mean±SD. Dotted lines connect results from siblings. Sets of values were compared using two-tailed t-test that yielded P=0.00054, P=0.0071, and P=00036 for comparisons in G, H, and I, respectively.
    Figure Legend Snippet: Genetic ablation of LRRC26 dramatically enhances susceptibility to DSS-induced colitis. A-D , Survival plots and change in body weight curves of mice treated for 7 days with 2.5% ( A-B ) or 3.5% DSS (MP Biomedicals, MW: 36-50 KDa) ( C-D ). In accordance with guidance from WUSTL-animal studies committee, animals whose body weight dropped > 30% of their initial value were considered terminal and euthanized. Graphs compile results from 4 independent assays where 8 litters (with both Lrrc26 -/- and WT littermates) were treated with 2.5% DSS or 2 assays where 4 litters were treated with 3.5%. Mice were 10-14 weeks old at the time of the treatment and both sexes were included. In body weight plots (mean ± SEM), for mice that died or required euthanasia, weight at the time of death was considered the same until the end of the experiment. Curves were compared using a two-way ANOVA that yielded P=0.0117 for 2.5% and P=0.0073 for 3.5% DSS. E , Representative images of H E stained most distal segment of colon from Lrrc26 - and WT (littermates) at the 7 th day of treatment (bars: 2.5 mm). F , Areas within the squares in (E), (bars 250 μm). G-I , Histophatologic colitis severity in the distal colon of DSS-treated mice scored from sections as in E-F. Inflammation score ( G ), total ulcer extention ( H ) and fraction of epithelial surface with 50% or more of crypt loss ( I ), represented as mean±SD. Dotted lines connect results from siblings. Sets of values were compared using two-tailed t-test that yielded P=0.00054, P=0.0071, and P=00036 for comparisons in G, H, and I, respectively.

    Techniques Used: Mouse Assay, Staining, Two Tailed Test

    3) Product Images from "HVEM Signalling Promotes Colitis"

    Article Title: HVEM Signalling Promotes Colitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0018495

    HVEM is required for DSS-induced pro-inflammatory cytokine and chemokine production. C57BL/6, HVEM -/- and LIGHT -/- mice were given 5% DSS in the drinking water for 4 days, then returned to normal drinking water. At day 6 mice were sacrificed and the colon removed for RNA isolation. (A) CCL3, (B) CCL4, (C) CCL5, (D) IFNγ, (E) TNFα, (F) IL-6 and (G) CXCL9 gene expression in the distal colon was analyzed by quantitative RT-PCR for C57BL/6 (black bar), HVEM -/- (grey bar) and LIGHT -/- (white bar) mice. For each individual sample, gene expression was normalized relative to β-Actin. Values represent fold increases in mRNA expression over the corresponding untreated controls. Means ± SD are shown for two independent experiments (n = 8–10 mice per group) out of three independent experiments. Statistically significant differences between groups were assessed by a two tailed Student's t test: * p
    Figure Legend Snippet: HVEM is required for DSS-induced pro-inflammatory cytokine and chemokine production. C57BL/6, HVEM -/- and LIGHT -/- mice were given 5% DSS in the drinking water for 4 days, then returned to normal drinking water. At day 6 mice were sacrificed and the colon removed for RNA isolation. (A) CCL3, (B) CCL4, (C) CCL5, (D) IFNγ, (E) TNFα, (F) IL-6 and (G) CXCL9 gene expression in the distal colon was analyzed by quantitative RT-PCR for C57BL/6 (black bar), HVEM -/- (grey bar) and LIGHT -/- (white bar) mice. For each individual sample, gene expression was normalized relative to β-Actin. Values represent fold increases in mRNA expression over the corresponding untreated controls. Means ± SD are shown for two independent experiments (n = 8–10 mice per group) out of three independent experiments. Statistically significant differences between groups were assessed by a two tailed Student's t test: * p

    Techniques Used: Mouse Assay, Isolation, Expressing, Quantitative RT-PCR, Two Tailed Test

    HVEM -/- mice are resistant to DSS-induced colitis. C57BL/6, HVEM -/- and LIGHT -/- mice were given 5% DSS in the drinking water for 4 days, then returned to normal drinking water and sacrificed at day 6. (A) Body weight loss was monitored daily and is expressed as percentage change from initial body weight at day 0 for C57BL/6 (closed squares), HVEM -/- (open circles) or LIGHT -/- (closed circles). (B) Clinical activity scores were assessed at the time of sacrifice by a combination of total weight loss, stool character and occult blood. (C) Histological scores indicating immuno-pathology were calculated as described in the Materials and Methods and are shown for the distal part of the colon of individual mice for C57BL/6 (closed squares), HVEM -/- (open circles) or LIGHT -/- (closed circles). (D) Representative H E staining of distal colon tissue sections from control and DSS-treated mice. Scale bars are 10× magnification = 200 µm and 40× magnification = 0.05 µm. Data in (A), (B) and (C) represent the mean ± SD of one experiment (n = 4-6 mice per group) out of three independent experiments. Statistically significant differences between groups were assessed by a two tailed Student's t test: * p
    Figure Legend Snippet: HVEM -/- mice are resistant to DSS-induced colitis. C57BL/6, HVEM -/- and LIGHT -/- mice were given 5% DSS in the drinking water for 4 days, then returned to normal drinking water and sacrificed at day 6. (A) Body weight loss was monitored daily and is expressed as percentage change from initial body weight at day 0 for C57BL/6 (closed squares), HVEM -/- (open circles) or LIGHT -/- (closed circles). (B) Clinical activity scores were assessed at the time of sacrifice by a combination of total weight loss, stool character and occult blood. (C) Histological scores indicating immuno-pathology were calculated as described in the Materials and Methods and are shown for the distal part of the colon of individual mice for C57BL/6 (closed squares), HVEM -/- (open circles) or LIGHT -/- (closed circles). (D) Representative H E staining of distal colon tissue sections from control and DSS-treated mice. Scale bars are 10× magnification = 200 µm and 40× magnification = 0.05 µm. Data in (A), (B) and (C) represent the mean ± SD of one experiment (n = 4-6 mice per group) out of three independent experiments. Statistically significant differences between groups were assessed by a two tailed Student's t test: * p

    Techniques Used: Mouse Assay, Activity Assay, Staining, Two Tailed Test

    4) Product Images from "Effects of a small molecule R-spondin-1 substitute RS-246204 on a mouse intestinal organoid culture"

    Article Title: Effects of a small molecule R-spondin-1 substitute RS-246204 on a mouse intestinal organoid culture

    Journal: Oncotarget

    doi: 10.18632/oncotarget.23721

    Regenerative effects of RS-246204 in the DSS induced colitis model ( A ) Schedule of the experiment. ( B ) The relative body weight of the DSS induced colitis models treated with vehicle and RS-246204. n = 5; vehicle group, n = 6; RS-246204 group. ( C ) The expression levels of BrdU in the large intestinal epithelium were compared between the vehicle and RS-246204 groups. Bars, 50 μm. ( D ) The area of the BrdU-positive cells per high-power field was counted. n = 10; vehicle group, n = 15; RS-246204 group. Data are presented as the mean ± SEM; ** p
    Figure Legend Snippet: Regenerative effects of RS-246204 in the DSS induced colitis model ( A ) Schedule of the experiment. ( B ) The relative body weight of the DSS induced colitis models treated with vehicle and RS-246204. n = 5; vehicle group, n = 6; RS-246204 group. ( C ) The expression levels of BrdU in the large intestinal epithelium were compared between the vehicle and RS-246204 groups. Bars, 50 μm. ( D ) The area of the BrdU-positive cells per high-power field was counted. n = 10; vehicle group, n = 15; RS-246204 group. Data are presented as the mean ± SEM; ** p

    Techniques Used: Expressing

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    Article Snippet: .. Dextran 40,000 (MP Biomedicals ™, USA) was treated with sodium periodate (at the rate of 40 sodium periodate molecules per 1 dextran molecule) for 60 min in H2 O and then gel filtration was performed on a Sephadex G-25 column in 50 mM carbonate buffer (pH 8.6). .. Aldehyde functions formed were quantified by a colorimetric assay (546 nm) using 2,3,5-triphenyltetrazolium salts being converted into formazan.

    Mouse Assay:

    Article Title: Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis
    Article Snippet: The Indiana University School of Medicine–Northwest Institutional Animal Care and Use Committee approved all experiments with mice. .. Experimental colitis was induced in WT, Nod2 −/− , Pglyrp3 −/− , and Pglyrp3 −/− Nod2 −/− mice with 5% DSS (dextran sulfate sodium, MP Biomedical) in drinking water ( ). .. DSS-induced intestinal inflammation is a well-established animal model for colitis and its manifestations include bloody diarrhea, weight loss, shortening of the colon, mucosal ulceration, and epithelial dysplasia.

    Article Title: Effects of a small molecule R-spondin-1 substitute RS-246204 on a mouse intestinal organoid culture
    Article Snippet: After 5 days of TGF-β1 treatment, immunocytochemistry was performed to analyze the efficiency of the OEMT. .. Induction of DSS-induced colitis and administration of RS-246204Seven-week-old C57Bl/6 mice were given a 2.5% dextran sulfate sodium (DSS) (MP Biomedicals, Aurora, OH) treatment in drinking water for 5 days. ..

    Article Title: Distinct inactivated bacterial-based immune modulators vary in their therapeutic efficacies for treating disease based on the organ site of pathology
    Article Snippet: DSS/AOM model Mice were injected intraperitoneally on day 0 with azoxymethane (AOM; Sigma, Kawasaki, Kanagawa Prefecture, Japan), at 8 mg/kg body weight, 10 days after treatment started. .. After AOM injections, mice received 3 cycles of dextran sodium sulphate (DSS) (MP Biomedicals, Santa Ana, CA, USA) treatment spaced 3 weeks apart. .. In each cycle, DSS was administered in the drinking water ad libitum for 5 days at a final concentration of 2.5% weight/volume.

    Article Title: HVEM Signalling Promotes Colitis
    Article Snippet: .. DSS- induced experimental colitis Acute colitis was induced in age-matched C57BL/6, HVEM-/- , LIGHT-/- and RAG1-/- mice, by oral administration of Dextran sulfate sodium (DSS) (MP Biomedicals) at a concentration of 5% (w/v) in drinking water for 4 days. .. Age-matched C57BL/6, HVEM-/- and LIGHT-/- mice receiving normal drinking water served as controls.

    Article Title: Interleukin-7 enables antibody responses to bacterial polysaccharides by promoting B cell antigen receptor diversity
    Article Snippet: .. For dextran immunizations, 50 μg of α1-6 dextran (B512; MP Biomedicals, LLC, Solon, OH) or 50 μg of α1-3 dextran (B1355; A gift from Dr. A. Jeanes) dissolved in 100 μl DPBS were used to immunize mice i.p. For whole bacterial immunization, mice were injected i.p. with 3×108 heat-killed Escherichia coli strain W3110 pDC5, which expresses ViPS , or 108 heat-killed, paraformaldehyde-fixed Enterobacter cloacae strain MK7, which expresses α1-3 dextran. .. ViPS-specific IgM and IgG were measured by incubating 96-well microtiter plates (Nunc MultiSorp 467340; Thermo Fisher Scientific Nunc A/S, Roskilde, Denmark) with 2 μg/ml of ViPS in DPBS overnight at room temperature.

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice
    Article Snippet: For inhibitor studies, Caco-2 cells were pretreated for 10 min prior to addition of DSS with U0126 at 50 μM (Cell Signaling Technology; Beverly, MA) or Bay 11-7082 at 20μM (Calbiochem; San Diego, CA). .. Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days. ..

    Concentration Assay:

    Article Title: HVEM Signalling Promotes Colitis
    Article Snippet: .. DSS- induced experimental colitis Acute colitis was induced in age-matched C57BL/6, HVEM-/- , LIGHT-/- and RAG1-/- mice, by oral administration of Dextran sulfate sodium (DSS) (MP Biomedicals) at a concentration of 5% (w/v) in drinking water for 4 days. .. Age-matched C57BL/6, HVEM-/- and LIGHT-/- mice receiving normal drinking water served as controls.

    Injection:

    Article Title: Interleukin-7 enables antibody responses to bacterial polysaccharides by promoting B cell antigen receptor diversity
    Article Snippet: .. For dextran immunizations, 50 μg of α1-6 dextran (B512; MP Biomedicals, LLC, Solon, OH) or 50 μg of α1-3 dextran (B1355; A gift from Dr. A. Jeanes) dissolved in 100 μl DPBS were used to immunize mice i.p. For whole bacterial immunization, mice were injected i.p. with 3×108 heat-killed Escherichia coli strain W3110 pDC5, which expresses ViPS , or 108 heat-killed, paraformaldehyde-fixed Enterobacter cloacae strain MK7, which expresses α1-3 dextran. .. ViPS-specific IgM and IgG were measured by incubating 96-well microtiter plates (Nunc MultiSorp 467340; Thermo Fisher Scientific Nunc A/S, Roskilde, Denmark) with 2 μg/ml of ViPS in DPBS overnight at room temperature.

    Molecular Weight:

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice
    Article Snippet: For inhibitor studies, Caco-2 cells were pretreated for 10 min prior to addition of DSS with U0126 at 50 μM (Cell Signaling Technology; Beverly, MA) or Bay 11-7082 at 20μM (Calbiochem; San Diego, CA). .. Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days. ..

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  • dss  (Valiant)
    95
    Valiant dss
    Commensal-specific antibody production is increased in <t>Opg</t> −/− mice. a , d Schematic cartoons depicting experimental designs. Commensal-specific fecal or serum immunoglobulins were detected by flow cytometry. b , c , e , f Feces and sera were collected from Opg −/− or co-housed WT mice at day 0 ( b , e ) or day 5 ( c , f ) after the onset of <t>DSS</t> consumption. Flow cytometry assays of DAPI-positive fecal bacteria bound by the indicated Igs were performed without ( b , c ) or with ( e , f ) serum. Representative data from two independent experiments are shown as the mean ± standard deviation. * p
    Dss, supplied by Valiant, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dss/product/Valiant
    Average 95 stars, based on 1 article reviews
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    dss - by Bioz Stars, 2021-06
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    97
    Valiant dextran sodium sulfate dss
    Effects of KIOM-2015E on body weight, colon length, and myeloperoxidase (MPO) activity in the dextran sodium sulfate- <t>(DSS-)</t> induced colitis model. (a) Body weight. (b and c) Colon length. (d) Myeloperoxidase (MPO) activity. Mice were orally administered KIOM-2015E or <t>5-aminosalicylic</t> acid (5-ASA) (100 mg/kg) before DSS treatment. The body weights were monitored before KIOM-2015E or 5-ASA treatment during the experimental periods. The colonic lengths of mice were measured after being isolated from the sacrificed mice. The MPO activity was measured using MPO activity assay kit according to the manufacturer's protocol. The results represent the mean ± standard error of the mean values of each mouse in the same group. ∗∗∗ p
    Dextran Sodium Sulfate Dss, supplied by Valiant, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dextran sodium sulfate dss/product/Valiant
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dextran sodium sulfate dss - by Bioz Stars, 2021-06
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    Commensal-specific antibody production is increased in Opg −/− mice. a , d Schematic cartoons depicting experimental designs. Commensal-specific fecal or serum immunoglobulins were detected by flow cytometry. b , c , e , f Feces and sera were collected from Opg −/− or co-housed WT mice at day 0 ( b , e ) or day 5 ( c , f ) after the onset of DSS consumption. Flow cytometry assays of DAPI-positive fecal bacteria bound by the indicated Igs were performed without ( b , c ) or with ( e , f ) serum. Representative data from two independent experiments are shown as the mean ± standard deviation. * p

    Journal: Nature Communications

    Article Title: Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity

    doi: 10.1038/s41467-019-13883-y

    Figure Lengend Snippet: Commensal-specific antibody production is increased in Opg −/− mice. a , d Schematic cartoons depicting experimental designs. Commensal-specific fecal or serum immunoglobulins were detected by flow cytometry. b , c , e , f Feces and sera were collected from Opg −/− or co-housed WT mice at day 0 ( b , e ) or day 5 ( c , f ) after the onset of DSS consumption. Flow cytometry assays of DAPI-positive fecal bacteria bound by the indicated Igs were performed without ( b , c ) or with ( e , f ) serum. Representative data from two independent experiments are shown as the mean ± standard deviation. * p

    Article Snippet: Opg −/− mice and co-housed WT mice or littermate Opg +/− mice consumed DSS (dextran sulfate sodium salt; MP Biomedicals LLC., Santa Ana, CA, USA) in their drinking water for up to 8 days.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Standard Deviation

    Absence of Opg ameliorates DSS-induced colitis. a Daily changes in body weight during dextran sodium sulfate (DSS)-induced colitis. Changes in body weight percentage were calculated by dividing the body weight on the specified day by the body weight at day 0. b Colon length was measured after sacrifice at day 8. c Representative histology of hematoxylin- and eosin-stained colonic tissue from WT and Opg −/− mice with DSS-induced colitis on day 8. d Stool scores were measured as described in the Methods. e Spleen weight was measured after sacrifice at day 8. a , b , d , and e Data are presented as the mean ± SEM. ** p

    Journal: Nature Communications

    Article Title: Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity

    doi: 10.1038/s41467-019-13883-y

    Figure Lengend Snippet: Absence of Opg ameliorates DSS-induced colitis. a Daily changes in body weight during dextran sodium sulfate (DSS)-induced colitis. Changes in body weight percentage were calculated by dividing the body weight on the specified day by the body weight at day 0. b Colon length was measured after sacrifice at day 8. c Representative histology of hematoxylin- and eosin-stained colonic tissue from WT and Opg −/− mice with DSS-induced colitis on day 8. d Stool scores were measured as described in the Methods. e Spleen weight was measured after sacrifice at day 8. a , b , d , and e Data are presented as the mean ± SEM. ** p

    Article Snippet: Opg −/− mice and co-housed WT mice or littermate Opg +/− mice consumed DSS (dextran sulfate sodium salt; MP Biomedicals LLC., Santa Ana, CA, USA) in their drinking water for up to 8 days.

    Techniques: Staining, Mouse Assay

    Non-hematopoietic cells in Opg −/− mice contribute to relief of DSS colitis symptoms. a , e Daily changes in body weight during dextran sodium sulfate (DSS)-induced colitis. Changes in body weight percentage were calculated by dividing the body weight on the specified day by the body weight at day 0. b , f Fecal clinical scores were measured as described in the Methods. c , g Colon length was measured after sacrifice at day 8. d , h Spleen weight was measured after sacrifice at day 8. WT or Opg −/− recipient mice following bone marrow transplantation from WT donors ( a – d ) and WT recipient mice following bone marrow transplantation from WT or Opg −/− donors ( e – h ) treated with 1.5% DSS for 7 days. Data are representative of two independent experiments. ** p

    Journal: Nature Communications

    Article Title: Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity

    doi: 10.1038/s41467-019-13883-y

    Figure Lengend Snippet: Non-hematopoietic cells in Opg −/− mice contribute to relief of DSS colitis symptoms. a , e Daily changes in body weight during dextran sodium sulfate (DSS)-induced colitis. Changes in body weight percentage were calculated by dividing the body weight on the specified day by the body weight at day 0. b , f Fecal clinical scores were measured as described in the Methods. c , g Colon length was measured after sacrifice at day 8. d , h Spleen weight was measured after sacrifice at day 8. WT or Opg −/− recipient mice following bone marrow transplantation from WT donors ( a – d ) and WT recipient mice following bone marrow transplantation from WT or Opg −/− donors ( e – h ) treated with 1.5% DSS for 7 days. Data are representative of two independent experiments. ** p

    Article Snippet: Opg −/− mice and co-housed WT mice or littermate Opg +/− mice consumed DSS (dextran sulfate sodium salt; MP Biomedicals LLC., Santa Ana, CA, USA) in their drinking water for up to 8 days.

    Techniques: Mouse Assay, Transplantation Assay

    Effects of KIOM-2015E on body weight, colon length, and myeloperoxidase (MPO) activity in the dextran sodium sulfate- (DSS-) induced colitis model. (a) Body weight. (b and c) Colon length. (d) Myeloperoxidase (MPO) activity. Mice were orally administered KIOM-2015E or 5-aminosalicylic acid (5-ASA) (100 mg/kg) before DSS treatment. The body weights were monitored before KIOM-2015E or 5-ASA treatment during the experimental periods. The colonic lengths of mice were measured after being isolated from the sacrificed mice. The MPO activity was measured using MPO activity assay kit according to the manufacturer's protocol. The results represent the mean ± standard error of the mean values of each mouse in the same group. ∗∗∗ p

    Journal: Journal of Immunology Research

    Article Title: Acer palmatum thumb. Ethanol Extract Alleviates Interleukin-6-Induced Barrier Dysfunction and Dextran Sodium Sulfate-Induced Colitis by Improving Intestinal Barrier Function and Reducing Inflammation

    doi: 10.1155/2018/5718396

    Figure Lengend Snippet: Effects of KIOM-2015E on body weight, colon length, and myeloperoxidase (MPO) activity in the dextran sodium sulfate- (DSS-) induced colitis model. (a) Body weight. (b and c) Colon length. (d) Myeloperoxidase (MPO) activity. Mice were orally administered KIOM-2015E or 5-aminosalicylic acid (5-ASA) (100 mg/kg) before DSS treatment. The body weights were monitored before KIOM-2015E or 5-ASA treatment during the experimental periods. The colonic lengths of mice were measured after being isolated from the sacrificed mice. The MPO activity was measured using MPO activity assay kit according to the manufacturer's protocol. The results represent the mean ± standard error of the mean values of each mouse in the same group. ∗∗∗ p

    Article Snippet: Dextran sodium sulfate (DSS), 5-aminosalicylic acid (5-ASA), and hematoxylin and eosin solutions were purchased from MP Biomedicals (Santa Ana, CA, USA) and Sigma Aldrich (St Louis, MO, USA).

    Techniques: Activity Assay, Mouse Assay, Isolation

    Levels of intestinal and fecal αGalCer MLI are influenced by diet (A), inflammation (B), and viral infection (C). A: Male C57BL/6N mice (23–36 weeks old) were fed for 7 days with control or WTD and total cecum was analyzed for the presence of αGalCer(d18:0/βh16:0), i.e., αGalCer MLI (n = 8). B: Male C57BL/6 mice (9–11 weeks old) were treated with DSS to induce colitis. After 2 and 4 days, liver and the content of the colon were isolated and analyzed for αGalCer MLI (control and 2d DSS, n = 3; 4d DSS, n = 5). C: Male C57BL/6 mice (8 weeks old) were infected with influence virus A. Total cecum and feces were analyzed at day 0 (mock-treated noninfected mice) and at day 7 (cecum, n = 6; feces, n = 5). Data of A and B were obtained with RP18-LC-MS 2 and data of C with the HILIC-MS 2 method. For absolute values see supplemental Fig. S9.

    Journal: Journal of Lipid Research

    Article Title: Bacterial immunogenic α-galactosylceramide identified in the murine large intestine: dependency on diet and inflammation [S]

    doi: 10.1194/jlr.RA119000236

    Figure Lengend Snippet: Levels of intestinal and fecal αGalCer MLI are influenced by diet (A), inflammation (B), and viral infection (C). A: Male C57BL/6N mice (23–36 weeks old) were fed for 7 days with control or WTD and total cecum was analyzed for the presence of αGalCer(d18:0/βh16:0), i.e., αGalCer MLI (n = 8). B: Male C57BL/6 mice (9–11 weeks old) were treated with DSS to induce colitis. After 2 and 4 days, liver and the content of the colon were isolated and analyzed for αGalCer MLI (control and 2d DSS, n = 3; 4d DSS, n = 5). C: Male C57BL/6 mice (8 weeks old) were infected with influence virus A. Total cecum and feces were analyzed at day 0 (mock-treated noninfected mice) and at day 7 (cecum, n = 6; feces, n = 5). Data of A and B were obtained with RP18-LC-MS 2 and data of C with the HILIC-MS 2 method. For absolute values see supplemental Fig. S9.

    Article Snippet: Induction of colitis in miceNine- to 11-week-old C57BL/6 male mice were given 2% dextran sodium sulfate (DSS) (colitis grade, molecular weight 36,000–50,000; MP Biomedicals GmbH, Eschwege, Germany) in drinking water and euthanized, respectively, after 2 or 4 days of treatment.

    Techniques: Infection, Mouse Assay, Isolation, Liquid Chromatography with Mass Spectroscopy, Hydrophilic Interaction Liquid Chromatography