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Biowest SAS dextran charcoal treated fbs
Release of NO from JS-K . (A) Generation of NO from JS-K in <t>RPMI-1640</t> medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.
Dextran Charcoal Treated Fbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dextran charcoal treated fbs/product/Biowest SAS
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dextran charcoal treated fbs - by Bioz Stars, 2021-04
86/100 stars

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1) Product Images from "JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells"

Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells

Journal: BMC Cancer

doi: 10.1186/1471-2407-12-130

Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.
Figure Legend Snippet: Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.

Techniques Used: Fluorescence, Microscopy, Staining

2) Product Images from "JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells"

Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells

Journal: BMC Cancer

doi: 10.1186/1471-2407-12-130

Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.
Figure Legend Snippet: Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.

Techniques Used: Fluorescence, Microscopy, Staining

Related Articles

Cell Culture:

Article Title: Genome-Wide Methylation Patterns in Androgen-Independent Prostate Cancer Cells: A Comprehensive Analysis Combining MeDIP-Bisulfite, RNA, and microRNA Sequencing Data
Article Snippet: LNCaP was chronically cultured in an androgen-deprived medium to establish an androgen-independent LNCaP line. .. First, LNCaP cells were cultured in an androgen-deprived medium composed of the phenol red-free dulbecco's modified eagle medium (DMEM)/F-12 medium (Gibco® ) plus 10% ( v / v ) charcoal/dextran-treated FBS (Biowest) for 5 passages. .. As flutamide was able to competitively inhibit the binding of androgens to AR, LNCaP cells were then cultured in the above androgen-deprived medium plus 10−7 mol/L flutamide (Schering-Plough, Kenilworth, NJ, USA) [ ] for 105 passages to obtain a complete androgen-independent cell line.

Modification:

Article Title: Genome-Wide Methylation Patterns in Androgen-Independent Prostate Cancer Cells: A Comprehensive Analysis Combining MeDIP-Bisulfite, RNA, and microRNA Sequencing Data
Article Snippet: LNCaP was chronically cultured in an androgen-deprived medium to establish an androgen-independent LNCaP line. .. First, LNCaP cells were cultured in an androgen-deprived medium composed of the phenol red-free dulbecco's modified eagle medium (DMEM)/F-12 medium (Gibco® ) plus 10% ( v / v ) charcoal/dextran-treated FBS (Biowest) for 5 passages. .. As flutamide was able to competitively inhibit the binding of androgens to AR, LNCaP cells were then cultured in the above androgen-deprived medium plus 10−7 mol/L flutamide (Schering-Plough, Kenilworth, NJ, USA) [ ] for 105 passages to obtain a complete androgen-independent cell line.

Incubation:

Article Title: Estrogen-related receptor alpha induces epithelial-mesenchymal transition through cancer-stromal interactions in endometrial cancer
Article Snippet: Plasmids (pSG5-empty, pSG5-ERRα, and pSG5-PGC-1α) were transfected using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. After a 4-h incubation, the transfection medium, containing Lipofectamine LTX and plasmids was removed and replaced with phenol red-free MEM supplemented with 10% dextran-coated charcoal-treated FBS (Biowest) and antibiotics. .. RNA interference The small interfering RNA (siRNA) for ERRα (s4831) and the negative control siRNA (control #1) were Silencer Select siRNAs purchased from Ambion (Austin, TX, USA).

Transfection:

Article Title: Estrogen-related receptor alpha induces epithelial-mesenchymal transition through cancer-stromal interactions in endometrial cancer
Article Snippet: Plasmids (pSG5-empty, pSG5-ERRα, and pSG5-PGC-1α) were transfected using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. After a 4-h incubation, the transfection medium, containing Lipofectamine LTX and plasmids was removed and replaced with phenol red-free MEM supplemented with 10% dextran-coated charcoal-treated FBS (Biowest) and antibiotics. .. RNA interference The small interfering RNA (siRNA) for ERRα (s4831) and the negative control siRNA (control #1) were Silencer Select siRNAs purchased from Ambion (Austin, TX, USA).

Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells
Article Snippet: The pRL-tk-LUC vector coding for a Renilla luciferase under control of a constitutively active thymidine kinase promoter was co-transfected (80 ng/well) to correct for transfection efficiency. .. After transfection cells were grown in RPMI 1640 with 5% dextran charcoal treated steroid free, dextran charcoal treated FBS (FBSdcc; Biowest, Nuaillé, France) and treated with 5 nM DHT and different concentrations of JS-K. (2) WNT-signalling : Luciferase reporter plasmids pTopFlash (250 ng/well) and pFopFlash (250 ng/well) were mixed either with pbCAT expression vector (250 ng/well) or insert-free vector (250 ng/well). pRL-tk-luc (80 ng/well) was co-transfected to correct for transfection efficiency. .. After an incubation period of 12 h in RPMI-1640 with 5% FBSdcc, medium was changed to RPMI-1640 containing 2.5% FBSdcc with increasing concentrations of JS-K. AR- and WNT-reporter activities were analyzed after 24 hours using the Dual Luciferase Reporter Assay System (Promega, Mannheim, Germany).

Luciferase:

Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells
Article Snippet: The pRL-tk-LUC vector coding for a Renilla luciferase under control of a constitutively active thymidine kinase promoter was co-transfected (80 ng/well) to correct for transfection efficiency. .. After transfection cells were grown in RPMI 1640 with 5% dextran charcoal treated steroid free, dextran charcoal treated FBS (FBSdcc; Biowest, Nuaillé, France) and treated with 5 nM DHT and different concentrations of JS-K. (2) WNT-signalling : Luciferase reporter plasmids pTopFlash (250 ng/well) and pFopFlash (250 ng/well) were mixed either with pbCAT expression vector (250 ng/well) or insert-free vector (250 ng/well). pRL-tk-luc (80 ng/well) was co-transfected to correct for transfection efficiency. .. After an incubation period of 12 h in RPMI-1640 with 5% FBSdcc, medium was changed to RPMI-1640 containing 2.5% FBSdcc with increasing concentrations of JS-K. AR- and WNT-reporter activities were analyzed after 24 hours using the Dual Luciferase Reporter Assay System (Promega, Mannheim, Germany).

Expressing:

Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells
Article Snippet: The pRL-tk-LUC vector coding for a Renilla luciferase under control of a constitutively active thymidine kinase promoter was co-transfected (80 ng/well) to correct for transfection efficiency. .. After transfection cells were grown in RPMI 1640 with 5% dextran charcoal treated steroid free, dextran charcoal treated FBS (FBSdcc; Biowest, Nuaillé, France) and treated with 5 nM DHT and different concentrations of JS-K. (2) WNT-signalling : Luciferase reporter plasmids pTopFlash (250 ng/well) and pFopFlash (250 ng/well) were mixed either with pbCAT expression vector (250 ng/well) or insert-free vector (250 ng/well). pRL-tk-luc (80 ng/well) was co-transfected to correct for transfection efficiency. .. After an incubation period of 12 h in RPMI-1640 with 5% FBSdcc, medium was changed to RPMI-1640 containing 2.5% FBSdcc with increasing concentrations of JS-K. AR- and WNT-reporter activities were analyzed after 24 hours using the Dual Luciferase Reporter Assay System (Promega, Mannheim, Germany).

Plasmid Preparation:

Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells
Article Snippet: The pRL-tk-LUC vector coding for a Renilla luciferase under control of a constitutively active thymidine kinase promoter was co-transfected (80 ng/well) to correct for transfection efficiency. .. After transfection cells were grown in RPMI 1640 with 5% dextran charcoal treated steroid free, dextran charcoal treated FBS (FBSdcc; Biowest, Nuaillé, France) and treated with 5 nM DHT and different concentrations of JS-K. (2) WNT-signalling : Luciferase reporter plasmids pTopFlash (250 ng/well) and pFopFlash (250 ng/well) were mixed either with pbCAT expression vector (250 ng/well) or insert-free vector (250 ng/well). pRL-tk-luc (80 ng/well) was co-transfected to correct for transfection efficiency. .. After an incubation period of 12 h in RPMI-1640 with 5% FBSdcc, medium was changed to RPMI-1640 containing 2.5% FBSdcc with increasing concentrations of JS-K. AR- and WNT-reporter activities were analyzed after 24 hours using the Dual Luciferase Reporter Assay System (Promega, Mannheim, Germany).

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    Biowest SAS dextran charcoal treated fbs
    Release of NO from JS-K . (A) Generation of NO from JS-K in <t>RPMI-1640</t> medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.
    Dextran Charcoal Treated Fbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dextran charcoal treated fbs/product/Biowest SAS
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dextran charcoal treated fbs - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Biowest SAS steroid free dextran charcoal treated fbs fbsdcc
    Release of NO from JS-K . (A) Generation of NO from JS-K in <t>RPMI-1640</t> medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.
    Steroid Free Dextran Charcoal Treated Fbs Fbsdcc, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steroid free dextran charcoal treated fbs fbsdcc/product/Biowest SAS
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    steroid free dextran charcoal treated fbs fbsdcc - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Biowest SAS dextran coated charcoal treated fbs
    Release of NO from JS-K . (A) Generation of NO from JS-K in <t>RPMI-1640</t> medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.
    Dextran Coated Charcoal Treated Fbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dextran coated charcoal treated fbs/product/Biowest SAS
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dextran coated charcoal treated fbs - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.

    Journal: BMC Cancer

    Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells

    doi: 10.1186/1471-2407-12-130

    Figure Lengend Snippet: Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.

    Article Snippet: LNCaP-SSR were routinely grown in RPMI-1640, 1% Penicillin/Streptomycin supplemented with 10% steroid free, dextran charcoal treated FBS (FBSdcc, BioWest, Nuaille, France) [ ].

    Techniques: Fluorescence, Microscopy, Staining