Journal: Nature Communications

Article Title: Ecophysiology and interactions of a taurine-respiring bacterium in the mouse gut

doi: 10.1038/s41467-023-41008-z

Figure Lengend Snippet: a 16 S rRNA gene tree and FISH probe coverage. Maximum likelihood branch supports (1000 resamplings) equal to or greater than 95% and 80% are indicated by black and gray circles, respectively. The scale bar indicates 0.1 estimated substitutions per residue. Accession numbers are shown in parentheses. Strain LT0009 is shown in bold and type strains are marked with a superscript ‘T’. Sequence sources are indicated with different colors (Supplementary Data ). Sequences were assigned to Taurinivorans and Taurinivorans muris based on the genus-level similarity cutoff of 94.5% and species-level similarity cutoff of 98.7% , respectively. The perfect-match coverage of probes TAU1151 for Taurinivorans and MAIL1151 for Mailhella is indicated. b Phylogenomic tree. Ultrafast bootstrap support values equal to or greater than 95% and 80% for the maximum likelihood tree are indicated with black and gray circles, respectively. Accession numbers are shown in parentheses. Strain LT0009 is shown in bold. Strains with complete genomes (genome size is indicated) are marked with a star. Genomes were assigned to Taurinivorans based on the genus-level AAI cutoff value of 63.4% . The scale bar indicates 0.1 estimated substitutions per residue. c Representative morphology of LT0009 cells in pure culture ( n = 3 independent cultures). SEM: Scanning electron microscopy images of cells of varying lengths. White arrows indicate the flagella. FISH: Cells hybridized with Cy3-labeled probe TAU1151 and Fluos-labeled probe EUB338mix and counterstained by DAPI. d Growth of strain LT0009 in modified Desulfovibrio medium confirmed complete utilization of taurine as electron acceptor concomitant with nearly stoichiometric production of sulfide. Electron donors L-lactate and pyruvate were provided in excess, and their utilization also contributed to acetate formation. Pyruvate in the medium and ammonia released from the deamination of taurine were not analyzed in this experiment. Lines represent averages of measures in triplicate cultures. Error bars represent one standard deviation ( n = 3 biologically independent cultures). Source data are provided as a Source Data file.

Article Snippet: Strain LT0009 respired thiosulfate, such as B. wadsworthia strain RZATAU , but lacks genes for PhsABC thiosulfate reductase and thiosulfate reductase from Desulfovibrio (EC 1.8.2.5) , which both (i) disproportionate thiosulfate to sulfide and sulfite and (ii) are present in the human B. wadsworthia strains ATCC 49260, 4_1_30, and 3_1_6.

Techniques: Residue, Sequencing, Electron Microscopy, Labeling, Modification, Standard Deviation