dermal microvascular endothelial cell  (ATCC)

Journal: Biomolecules

Article Title: Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

doi: 10.3390/biom14121590

Figure Lengend Snippet: MTS assay of HaCaT keratinocytes ( A – C ), human dermal fibroblasts (HDF) ( D – F ), and human dermal microvascular vein endothelial cells (HMVEC-d) ( G – I ) in the presence of Agrimonia eupatoria L. (AE) extract, lipophosphonoxin (LPPO) DR-6180, and combination of AE and LPPO. TGF-β1 was used as the positive control in HaCaT and HDF, while VEGF-A was used in HMVEC-d. The figures were generated using GraphPad Prism software (GraphPad Software, San Diego, CA, USA), which was also utilized to logarithmically transform the concentration values and generate fitting curves to visualize the data.

Article Snippet: Dermal microvascular endothelial cells (HMVEC-d) were obtained from Lonza (Lonza Walkersville, Inc., Walkersville, MD, USA).

Techniques: MTS Assay, Positive Control, Generated, Software, Concentration Assay

Journal: Biomolecules

Article Title: Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

doi: 10.3390/biom14121590

Figure Lengend Snippet: Wound healing (2D migration) assay of HaCaT keratinocytes ( A ) and human dermal microvascular vein endothelial cells—HMVEC-d ( B ) in the presence of Agrimonia eupatoria L. (AE) extract, lipophosphonoxin (LPPO) DR-6180, and combination of AE and LPPO. TGF-β1 was used as the positive control in HaCaT, while VEGF-A was used in HMVEC-d (Magnification 100×).

Article Snippet: Dermal microvascular endothelial cells (HMVEC-d) were obtained from Lonza (Lonza Walkersville, Inc., Walkersville, MD, USA).

Techniques: Migration, Positive Control

Journal: Biomolecules

Article Title: Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

doi: 10.3390/biom14121590

Figure Lengend Snippet: Western blot (WB) analysis of HaCaT keratinocytes ( A ), human dermal fibroblasts—HDF ( B ), and human dermal microvascular vein endothelial cells—HMVEC-d ( C ) in the presence of Agrimonia eupatoria L. (AE) extract, lipophosphonoxin (LPPO) DR-6180, and combination of AE and LPPO. TGF-β1 was used as the positive control in HaCaT and HDF, while VEGF-A was used in HMVEC-d.

Article Snippet: Dermal microvascular endothelial cells (HMVEC-d) were obtained from Lonza (Lonza Walkersville, Inc., Walkersville, MD, USA).

Techniques: Western Blot, Positive Control

Journal: Biomolecules

Article Title: Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

doi: 10.3390/biom14121590

Figure Lengend Snippet: MTS assay of HaCaT keratinocytes ( A – C ), human dermal fibroblasts (HDF) ( D – F ), and human dermal microvascular vein endothelial cells (HMVEC-d) ( G – I ) in the presence of Agrimonia eupatoria L. (AE) extract, lipophosphonoxin (LPPO) DR-6180, and combination of AE and LPPO. TGF-β1 was used as the positive control in HaCaT and HDF, while VEGF-A was used in HMVEC-d. The figures were generated using GraphPad Prism software (GraphPad Software, San Diego, CA, USA), which was also utilized to logarithmically transform the concentration values and generate fitting curves to visualize the data.

Article Snippet: In in vitro experiments conducted on human dermal microvascular endothelial cells, VEGF-A from the Endopan MV Kit (PAN-Biotech GmbH, Aidenbach, Germany) was used as a positive control to assess the cells’ ability to undergo calcium-dependent homophilic binding at adherens junctions [ ].

Techniques: MTS Assay, Positive Control, Generated, Software, Concentration Assay

Journal: Biomolecules

Article Title: Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

doi: 10.3390/biom14121590

Figure Lengend Snippet: Wound healing (2D migration) assay of HaCaT keratinocytes ( A ) and human dermal microvascular vein endothelial cells—HMVEC-d ( B ) in the presence of Agrimonia eupatoria L. (AE) extract, lipophosphonoxin (LPPO) DR-6180, and combination of AE and LPPO. TGF-β1 was used as the positive control in HaCaT, while VEGF-A was used in HMVEC-d (Magnification 100×).

Article Snippet: In in vitro experiments conducted on human dermal microvascular endothelial cells, VEGF-A from the Endopan MV Kit (PAN-Biotech GmbH, Aidenbach, Germany) was used as a positive control to assess the cells’ ability to undergo calcium-dependent homophilic binding at adherens junctions [ ].

Techniques: Migration, Positive Control

Journal: Biomolecules

Article Title: Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

doi: 10.3390/biom14121590

Figure Lengend Snippet: Western blot (WB) analysis of HaCaT keratinocytes ( A ), human dermal fibroblasts—HDF ( B ), and human dermal microvascular vein endothelial cells—HMVEC-d ( C ) in the presence of Agrimonia eupatoria L. (AE) extract, lipophosphonoxin (LPPO) DR-6180, and combination of AE and LPPO. TGF-β1 was used as the positive control in HaCaT and HDF, while VEGF-A was used in HMVEC-d.

Article Snippet: In in vitro experiments conducted on human dermal microvascular endothelial cells, VEGF-A from the Endopan MV Kit (PAN-Biotech GmbH, Aidenbach, Germany) was used as a positive control to assess the cells’ ability to undergo calcium-dependent homophilic binding at adherens junctions [ ].

Techniques: Western Blot, Positive Control

Journal: Nature Communications

Article Title: Endothelial Pim3 kinase protects the vascular barrier during lung metastasis

doi: 10.1038/s41467-024-54445-1

Figure Lengend Snippet: a shPIM3 or control (shScr) silenced human umbilical vein endothelial cells (HUVECs) and dermal microvascular endothelial cells (BECs) were stained for vascular endothelial cadherin (CDH5) and F-actin. Nuclei were stained using DAPI. Relative CDH5 signal intensity ( b ) and area ( c ) (normalized to number of nuclei) in HUVEC ( n = 3 independent experiments for shScr, n = 6 independent experiments for shPIM3) and in BEC ( n = 3 for shScr, n = 5 for shPIM3). Western blot ( d ) and quantification ( e ) of CDH5 in HUVECs treated as in ( a ). n = 3 independent experiments for shScr, n = 6 for shPIM3. f shPIM3 or shScr silenced BECs were stained for α- and β-catenin (CTNNA1 and CTNNB1) and HUVECs for δ-catenin (CTNND1) and F-actin. Nuclei were stained using DAPI. Relative α-catenin ( g , h ), β-catenin ( i , j ) and δ-catenin ( k , l ) signal intensities (per field) and area (normalized to number of nuclei) relative to control (shScr). n = 3 for shScr, n = 6 for shPIM3 ( g – j ). n = 3 for shScr, n = 5 for shPIM3 ( k , l ). m Schematic representation of CDH5 and α-, β- and δ-catenin in adherens junctions created in Biorender.com. Two-tailed unpaired t -test ( b , c , e , g – l ). Data are presented as mean values + /- SD. Data is pooled from independent experiments using two shPIM3 clones in b , c , e , g – l . Scale bars 50 μm ( a , f ); 25 μm in close-up images ( a , f ). Source data are provided as a Source Data file.

Article Snippet: Human Umbilical Vein ECs (HUVEC) (Cell Applications, Inc., San Diego, CA, USA) and Human Dermal Blood Microvascular Endothelial Cells (a.k.a.

Techniques: Control, Staining, Western Blot, Two Tailed Test, Clone Assay

Journal: Nature Communications

Article Title: Endothelial Pim3 kinase protects the vascular barrier during lung metastasis

doi: 10.1038/s41467-024-54445-1

Figure Lengend Snippet: Control or AZD-1208 treated human umbilical vein endothelial cells (HUVEC) ( a ) and human dermal microvascular blood endothelial cells (BEC) ( b ) were analyzed using ECIS electrical cell impedance sensing. Arrows indicate initiation of serum starvation (arrow 1) and initiation of treatment (arrow 2). Shown are representative experiments with triplicate samples with SEM. Significant differences based on three independent experiments ( n = 3, Ctrl vs AZD-1208 at indicated times after treatment initiation) for HUVEC at 24 h 1 µM ( p = 0.0372), 10 µM ( p = 0.0026), at 48 h 1 µM ( p = 0.0456), 10 µM ( p < 0.0001) and at 72 h 10 µM ( p = 0.0053) and for BEC at 48 h 1 µM ( p = 0.0086), 10 µM ( p = 0.0012). c Representative images of HUVEC and BEC treated with AZD-1208 (1 μM) or 0.1% DMSO (Ctrl) for 24 h in reduced 2.5% serum, and stained for CDH5, F-actin and nuclei (DAPI). Relative CDH5 signal intensity (per field) ( d ) and area (normalized to number of nuclei) ( e ). n = 3 independent experiments. f , g CDH5 Western blot and quantification of HUVEC treated as in ( c ). n = 3 independent experiments. h AZD-1208 (30 mg kg −1 ) or vehicle (Ctrl) was orally administered daily for 5 days. CDH5 and collagen IV (Col IV) were stained in thick lung sections. Shown are maximum intensity projections of confocal z-stacks. i Magnification of maximum intensity projection of CDH5 stained lung sections (top) with surface masking (below). Arrows indicate gaps in CDH5 staining. Quantification of CDH5 intensity ( j ) and area ( k ), and number of gaps in CDH5 staining ( l ) as explained in materials and methods. n = 4 independent experiments. Mixed-effects analysis ( a ) and two-way ANOVA ( b ) both with multiple comparisons, two-tailed unpaired t -test ( d , e , g , j , l ), two-sided Mann-Whitney U test ( k ). Data are presented as mean values + /- SD ( d , e , g , j – l ,) or + /- SEM ( a , b ). Scale bars 50 µm ( c ); 25 µm ( h , close-up images in c ) and 10 µm ( i ). Source data are provided as a Source Data file.

Article Snippet: Human Umbilical Vein ECs (HUVEC) (Cell Applications, Inc., San Diego, CA, USA) and Human Dermal Blood Microvascular Endothelial Cells (a.k.a.

Techniques: Control, Staining, Western Blot, Two Tailed Test, MANN-WHITNEY