deparaffinized sections  (Vector Laboratories)


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    Name:
    Antigen Unmasking Solution Citric Acid Based
    Description:
    Vector s Antigen Unmasking Solutions are highly effective at revealing antigens in formalin fixed paraffin embedded tissue sections when used in combination with a high temperature treatment procedure This citrate based solution is pH 6 0 and is supplied as convenient 100x concentrated stock sufficient for preparation of 25 L of working solution
    Catalog Number:
    h-3300
    Price:
    None
    Category:
    Histology reagents or solutions or stains
    Size:
    250 ml
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    Structured Review

    Vector Laboratories deparaffinized sections
    Antigen Unmasking Solution Citric Acid Based
    Vector s Antigen Unmasking Solutions are highly effective at revealing antigens in formalin fixed paraffin embedded tissue sections when used in combination with a high temperature treatment procedure This citrate based solution is pH 6 0 and is supplied as convenient 100x concentrated stock sufficient for preparation of 25 L of working solution
    https://www.bioz.com/result/deparaffinized sections/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinized sections - by Bioz Stars, 2021-03
    86/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Augmentation of vaccine-induced humoral and cellular immunity by a physical radiofrequency adjuvant
    Article Snippet: Paraffin sections were subjected to standard hematoxylin and eosin staining to visualize microscopic structures or Trichrome staining to visualize dermal collagen levels. .. For IHC, paraffin sections were deparaffinized and then subjected to heat-induced epitope retrieval with antigen unmasking solution (H-3300, Vector Laboratories). ..

    Article Title: Expression Patterns of VEGF and Flk-1 in Human Endometrium during the Menstrual Cycle
    Article Snippet: .. Immunohistochemistry Tissue sections were dewaxed through descending grades of ethanol to distilled water, and pretreated with citra buffer (Vector H3300, Vector Laboratories, Burlingame, CA) in a steamer (HA900; Black & Decker, Hampstead, MD) at 90°C for 20 min . .. Immunohisto-chemical staining was then performed using monoclonal antibodies of anti mouse VEGF (sc-7269) and anti-human Flk-1(sc-6251) (Santa Cruz Biotechnology Inc, CA).

    Article Title: The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma
    Article Snippet: .. IHC and immunofluorescence followed our established procedures ( ; ; ), including high-temperature antigen retrieval (Vector Unmasking Solution; Vector Laboratories, Burlingame, CA) prior to staining paraffin sections. ..

    Staining:

    Article Title: Pre-existing smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesions
    Article Snippet: Secondary antibodies were Alexa 488 goat anti-chicken, Alexa 546 goat anti-rabbit, and Alex 546 goat anti-rat, all purchased from Life Technologies (Grand Island, NY). .. Antigen retrieval was required for CD31 and β-galactosidase staining and achieved by incubating sections with citrate acid (H-3300, Vector Labs, Burlingame, CA) in a pressure cooker for 10 min. All assays were evaluated with confocal microscopy to determine co-localization of the examined markers. .. The R26R+ ;Myh11-CreER+ or EGFP+ marrow cells (on a Ly5.2 background) were harvested from femurs by flushing with buffer-containing PBS plus 2%FBS.

    Article Title: The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma
    Article Snippet: .. IHC and immunofluorescence followed our established procedures ( ; ; ), including high-temperature antigen retrieval (Vector Unmasking Solution; Vector Laboratories, Burlingame, CA) prior to staining paraffin sections. ..

    Article Title: Subcortical TDP-43 pathology occurs infrequently in multiple system atrophy
    Article Snippet: The following primary antibodies were used: mouse anti-paired helical filament (PHF1) monoclonal antibody (mAb; a gift of Peter Davies; 1:1000), mouse anti-ubiquitin mAb (1510, Chemicon, Temecula, CA, USA; 1:100,000), rabbit polyclonal anti-TDP-43 (Protein-Tech Group, Chicago, IL, USA; 1:4,500), rat anti-phosphorylated TDP-43 mAb (S409/410 [ ], 1:1000), mouse anti-α-synuclein mAb (Syn303, generated in CNDR, Philadelphia, PA; 1:4000), rabbit polyclonal anti-FUS antibody (Sigma-Aldrich, Saint Louis, MO, US, 1:400). .. Sections stained for ubiquitin, TDP-43, and FUS were pre-treated by boiling in citrate antigen unmasking solution (Vector Laboratories Burlingame, CA, USA, 1:100) using a microwave, and those stained for α-synuclein were pretreated with 80% formic acid (as was a subset of sections stained for TDP-43). .. Double-labeling immunofluorescence IHC using Alexa Fluor 488 and 594 conjugated secondary antibodies (Molecular Probes, Eugene, OR, USA) was performed as previously described [ , ].

    Confocal Microscopy:

    Article Title: Pre-existing smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesions
    Article Snippet: Secondary antibodies were Alexa 488 goat anti-chicken, Alexa 546 goat anti-rabbit, and Alex 546 goat anti-rat, all purchased from Life Technologies (Grand Island, NY). .. Antigen retrieval was required for CD31 and β-galactosidase staining and achieved by incubating sections with citrate acid (H-3300, Vector Labs, Burlingame, CA) in a pressure cooker for 10 min. All assays were evaluated with confocal microscopy to determine co-localization of the examined markers. .. The R26R+ ;Myh11-CreER+ or EGFP+ marrow cells (on a Ly5.2 background) were harvested from femurs by flushing with buffer-containing PBS plus 2%FBS.

    Plasmid Preparation:

    Article Title: Expression Patterns of VEGF and Flk-1 in Human Endometrium during the Menstrual Cycle
    Article Snippet: .. Immunohistochemistry Tissue sections were dewaxed through descending grades of ethanol to distilled water, and pretreated with citra buffer (Vector H3300, Vector Laboratories, Burlingame, CA) in a steamer (HA900; Black & Decker, Hampstead, MD) at 90°C for 20 min . .. Immunohisto-chemical staining was then performed using monoclonal antibodies of anti mouse VEGF (sc-7269) and anti-human Flk-1(sc-6251) (Santa Cruz Biotechnology Inc, CA).

    Article Title: The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma
    Article Snippet: .. IHC and immunofluorescence followed our established procedures ( ; ; ), including high-temperature antigen retrieval (Vector Unmasking Solution; Vector Laboratories, Burlingame, CA) prior to staining paraffin sections. ..

    Immunofluorescence:

    Article Title: The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma
    Article Snippet: .. IHC and immunofluorescence followed our established procedures ( ; ; ), including high-temperature antigen retrieval (Vector Unmasking Solution; Vector Laboratories, Burlingame, CA) prior to staining paraffin sections. ..

    Transferring:

    Article Title: Gene-Specific Genetic Complementation between Brca1 and Cobra1 During Mouse Mammary Gland Development
    Article Snippet: 3 µM paraffin section slides were first de-paraffinized with xylene, and then rehydrated in descending grade of alcohol (100%, 95%, 70%, and 50%). .. Samples were washed briefly with PBS before transferring to boiling antigen-unmasking solution (Vector Labs, H-3300) for 20 min. Endogenous peroxidase was blocked by pre-incubating slides in 3% hydrogen peroxide for 10 min followed by 10% normal goat serum in PBS for 1 hr blocking at room temperature. ..

    Blocking Assay:

    Article Title: Gene-Specific Genetic Complementation between Brca1 and Cobra1 During Mouse Mammary Gland Development
    Article Snippet: 3 µM paraffin section slides were first de-paraffinized with xylene, and then rehydrated in descending grade of alcohol (100%, 95%, 70%, and 50%). .. Samples were washed briefly with PBS before transferring to boiling antigen-unmasking solution (Vector Labs, H-3300) for 20 min. Endogenous peroxidase was blocked by pre-incubating slides in 3% hydrogen peroxide for 10 min followed by 10% normal goat serum in PBS for 1 hr blocking at room temperature. ..

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  • 93
    Vector Laboratories dba lectin
    Effect of fluid therapy on glycocalyx preservation. Representative immunofluorescent staining of the glomeruli using <t>DBA</t> <t>lectin</t> in the HVFT group (A) and the IGDT group (B). C, Mean fluorescent intensity of the glomeruli after staining with DBA lectin in the HVFT (n = 14) and IGDT groups (n = 14). Error bars represent SD. DBA indicates Dolichos biflorus agglutinin; HVFT, high-volume fluid infusion therapy; IGDT, individual goal-directed fluid therapy; MFI, mean fluorescent intensity; SD, standard deviation.
    Dba Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dba lectin/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dba lectin - by Bioz Stars, 2021-03
    93/100 stars
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    93
    Vector Laboratories fluorescein labeled succinylated wheat germ agglutinin
    Relationship between O-GlcNAcylation and RVD in PAH patients. ( A ) Correlation between HgbA1C and right ventricular contractility in PAH patients. There is a significant negative relationship between HgbA1C and right ventricular contractility ( r = −0.52, p = 0.02) ( B ) Diabetic patients have lower right ventricular contractility than nondiabetic PAH patients (12.5 ± 4.5 s −1 vs. 17.5 ± 6.0 s −1 , p = 0.01). There were no significant differences in pulmonary vascular disease severity in diabetic versus nondiabetic patients as quantified by mean pulmonary artery pressure (48 ± 12 vs. 48 ± 16 mmHg, p = 0.37) ( C ), pulmonary vascular resistance (8.3 ± 4.9 vs. 8.9 ± 5.5 Wood units, p = 0.68) ( D ), and pulmonary arterial compliance (1.8 ± 1.0 vs. 1.8 ± 1.2 mL/mm Hg, p = 0.64) ( E ). ( F ) Representative confocal micrographs of right ventricular sections stained with <t>succinylated</t> wheat germ agglutinin (WGA). ( G ) Quantification of succinylated WGA signal intensity in right ventricular cardiomyocytes from two control ( n = 63 total cells analyzed) and two PAH biopsy specimens ( n = 55 total cells analyzed). Green: Succinylated WGA, Blue: DAPI, and Magenta: WGA. * indicates significantly different as determined by t -test in ( B ) or Mann–Whitney U-test in ( G ).
    Fluorescein Labeled Succinylated Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein labeled succinylated wheat germ agglutinin/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein labeled succinylated wheat germ agglutinin - by Bioz Stars, 2021-03
    93/100 stars
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    97
    Vector Laboratories isolectin b4
    Mybl2 gene transfer effect on performance of EDCs in vivo. (a) A schematic overview of the experiment. (b) Improvement in heart function (∆EF %) at 3 weeks after EDC injection compared to baseline evaluation at Day 7 when Mybl2 over‐expressing EDC were transplanted into LCA ligated mouse heart ( n = 9) (c) Scar size and representative pictures of Masson’s Trichrome staining ( n = 5). (d) Quantification of vessel number in the border zone infarct area and representative images of <t>isolectin</t> B4 staining ( n = 5). (e) Effect of Mybl2 over‐expression on EDC retention. Representative images of bioluminescence signal evaluated 1, 3, 5, and 7 days after EDC injection (right panel) and the percentage of retained cells determined by calculating the signal ratio to Day 1 (left panel; n = 6). Values are mean ± SEM . * p ≤ .05 as indicated; In E# p
    Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isolectin b4/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isolectin b4 - by Bioz Stars, 2021-03
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    Image Search Results


    Effect of fluid therapy on glycocalyx preservation. Representative immunofluorescent staining of the glomeruli using DBA lectin in the HVFT group (A) and the IGDT group (B). C, Mean fluorescent intensity of the glomeruli after staining with DBA lectin in the HVFT (n = 14) and IGDT groups (n = 14). Error bars represent SD. DBA indicates Dolichos biflorus agglutinin; HVFT, high-volume fluid infusion therapy; IGDT, individual goal-directed fluid therapy; MFI, mean fluorescent intensity; SD, standard deviation.

    Journal: Anesthesia and Analgesia

    Article Title: Goal-Directed Fluid Therapy Does Not Improve Early Glomerular Filtration Rate in a Porcine Renal Transplantation Model

    doi: 10.1213/ANE.0000000000004453

    Figure Lengend Snippet: Effect of fluid therapy on glycocalyx preservation. Representative immunofluorescent staining of the glomeruli using DBA lectin in the HVFT group (A) and the IGDT group (B). C, Mean fluorescent intensity of the glomeruli after staining with DBA lectin in the HVFT (n = 14) and IGDT groups (n = 14). Error bars represent SD. DBA indicates Dolichos biflorus agglutinin; HVFT, high-volume fluid infusion therapy; IGDT, individual goal-directed fluid therapy; MFI, mean fluorescent intensity; SD, standard deviation.

    Article Snippet: Sections were deparaffinized and incubated with DBA lectin (VEC-B-1035; VectorLabs, Burlingame, CA) and further incubation with fluorescein isothiocyanate (FITC)-conjugated streptavidin.

    Techniques: Preserving, Staining, Standard Deviation

    Relationship between O-GlcNAcylation and RVD in PAH patients. ( A ) Correlation between HgbA1C and right ventricular contractility in PAH patients. There is a significant negative relationship between HgbA1C and right ventricular contractility ( r = −0.52, p = 0.02) ( B ) Diabetic patients have lower right ventricular contractility than nondiabetic PAH patients (12.5 ± 4.5 s −1 vs. 17.5 ± 6.0 s −1 , p = 0.01). There were no significant differences in pulmonary vascular disease severity in diabetic versus nondiabetic patients as quantified by mean pulmonary artery pressure (48 ± 12 vs. 48 ± 16 mmHg, p = 0.37) ( C ), pulmonary vascular resistance (8.3 ± 4.9 vs. 8.9 ± 5.5 Wood units, p = 0.68) ( D ), and pulmonary arterial compliance (1.8 ± 1.0 vs. 1.8 ± 1.2 mL/mm Hg, p = 0.64) ( E ). ( F ) Representative confocal micrographs of right ventricular sections stained with succinylated wheat germ agglutinin (WGA). ( G ) Quantification of succinylated WGA signal intensity in right ventricular cardiomyocytes from two control ( n = 63 total cells analyzed) and two PAH biopsy specimens ( n = 55 total cells analyzed). Green: Succinylated WGA, Blue: DAPI, and Magenta: WGA. * indicates significantly different as determined by t -test in ( B ) or Mann–Whitney U-test in ( G ).

    Journal: International Journal of Molecular Sciences

    Article Title: Excess Protein O-GlcNAcylation Links Metabolic Derangements to Right Ventricular Dysfunction in Pulmonary Arterial Hypertension

    doi: 10.3390/ijms21197278

    Figure Lengend Snippet: Relationship between O-GlcNAcylation and RVD in PAH patients. ( A ) Correlation between HgbA1C and right ventricular contractility in PAH patients. There is a significant negative relationship between HgbA1C and right ventricular contractility ( r = −0.52, p = 0.02) ( B ) Diabetic patients have lower right ventricular contractility than nondiabetic PAH patients (12.5 ± 4.5 s −1 vs. 17.5 ± 6.0 s −1 , p = 0.01). There were no significant differences in pulmonary vascular disease severity in diabetic versus nondiabetic patients as quantified by mean pulmonary artery pressure (48 ± 12 vs. 48 ± 16 mmHg, p = 0.37) ( C ), pulmonary vascular resistance (8.3 ± 4.9 vs. 8.9 ± 5.5 Wood units, p = 0.68) ( D ), and pulmonary arterial compliance (1.8 ± 1.0 vs. 1.8 ± 1.2 mL/mm Hg, p = 0.64) ( E ). ( F ) Representative confocal micrographs of right ventricular sections stained with succinylated wheat germ agglutinin (WGA). ( G ) Quantification of succinylated WGA signal intensity in right ventricular cardiomyocytes from two control ( n = 63 total cells analyzed) and two PAH biopsy specimens ( n = 55 total cells analyzed). Green: Succinylated WGA, Blue: DAPI, and Magenta: WGA. * indicates significantly different as determined by t -test in ( B ) or Mann–Whitney U-test in ( G ).

    Article Snippet: Deparaffinized and rehydrated sections were incubated with fluorescein labeled succinylated wheat germ agglutinin (Vector Laboratories, Burlingame, CA, USA) (1:50) and Alexa 633-WGA overnight at 4 °C, washed in PBS twice, treated with autofluorescence quenching kit (Vector Laboratories), and mounted in Antifade media containing DAPI (Vector Laboratories).

    Techniques: Staining, Whole Genome Amplification, MANN-WHITNEY

    Mybl2 gene transfer effect on performance of EDCs in vivo. (a) A schematic overview of the experiment. (b) Improvement in heart function (∆EF %) at 3 weeks after EDC injection compared to baseline evaluation at Day 7 when Mybl2 over‐expressing EDC were transplanted into LCA ligated mouse heart ( n = 9) (c) Scar size and representative pictures of Masson’s Trichrome staining ( n = 5). (d) Quantification of vessel number in the border zone infarct area and representative images of isolectin B4 staining ( n = 5). (e) Effect of Mybl2 over‐expression on EDC retention. Representative images of bioluminescence signal evaluated 1, 3, 5, and 7 days after EDC injection (right panel) and the percentage of retained cells determined by calculating the signal ratio to Day 1 (left panel; n = 6). Values are mean ± SEM . * p ≤ .05 as indicated; In E# p

    Journal: Aging Cell

    Article Title: Mybl2 rejuvenates heart explant‐derived cells from aged donors after myocardial infarction, et al. Mybl2 rejuvenates heart explant‐derived cells from aged donors after myocardial infarction

    doi: 10.1111/acel.13174

    Figure Lengend Snippet: Mybl2 gene transfer effect on performance of EDCs in vivo. (a) A schematic overview of the experiment. (b) Improvement in heart function (∆EF %) at 3 weeks after EDC injection compared to baseline evaluation at Day 7 when Mybl2 over‐expressing EDC were transplanted into LCA ligated mouse heart ( n = 9) (c) Scar size and representative pictures of Masson’s Trichrome staining ( n = 5). (d) Quantification of vessel number in the border zone infarct area and representative images of isolectin B4 staining ( n = 5). (e) Effect of Mybl2 over‐expression on EDC retention. Representative images of bioluminescence signal evaluated 1, 3, 5, and 7 days after EDC injection (right panel) and the percentage of retained cells determined by calculating the signal ratio to Day 1 (left panel; n = 6). Values are mean ± SEM . * p ≤ .05 as indicated; In E# p

    Article Snippet: A separate set of sections were deparaffinized and blocked prior to staining for isolectin B4 (B‐1205, Vector Laboratories), alpha smooth muscle acting (ab5694, Abcam), or von Willebrand factor (11778‐1‐AP, Proteintech).

    Techniques: In Vivo, Injection, Expressing, Staining, Over Expression

    Evaluation of EDC performance in vivo. (a) A schematic overview of the experiment. (b) LVEF functional difference (∆EF %) at 3 weeks after EDC injection compared to baseline evaluation at Day 7 ( n = 12). (c) Scar size evaluation calculated using Masson’s trichrome staining by measuring fibrotic tissue relative to the whole ventricular area on heart sections at 3 weeks post‐EDC treatment ( n = 6). Representative images of Masson’s trichrome staining; left panel (d) The number of vessels per field of view in the infarct border zone calculated after isolectin B4 staining ( n = 6). Representative images of isolectin B4 staining; left panel Ctl, control; ICM, ischemic; Veh, vehicle. Values are mean ± SEM . * p ≤ .05 as indicated. EDCs, explant‐derived cells; LVEF, left ventricular ejection fraction

    Journal: Aging Cell

    Article Title: Mybl2 rejuvenates heart explant‐derived cells from aged donors after myocardial infarction, et al. Mybl2 rejuvenates heart explant‐derived cells from aged donors after myocardial infarction

    doi: 10.1111/acel.13174

    Figure Lengend Snippet: Evaluation of EDC performance in vivo. (a) A schematic overview of the experiment. (b) LVEF functional difference (∆EF %) at 3 weeks after EDC injection compared to baseline evaluation at Day 7 ( n = 12). (c) Scar size evaluation calculated using Masson’s trichrome staining by measuring fibrotic tissue relative to the whole ventricular area on heart sections at 3 weeks post‐EDC treatment ( n = 6). Representative images of Masson’s trichrome staining; left panel (d) The number of vessels per field of view in the infarct border zone calculated after isolectin B4 staining ( n = 6). Representative images of isolectin B4 staining; left panel Ctl, control; ICM, ischemic; Veh, vehicle. Values are mean ± SEM . * p ≤ .05 as indicated. EDCs, explant‐derived cells; LVEF, left ventricular ejection fraction

    Article Snippet: A separate set of sections were deparaffinized and blocked prior to staining for isolectin B4 (B‐1205, Vector Laboratories), alpha smooth muscle acting (ab5694, Abcam), or von Willebrand factor (11778‐1‐AP, Proteintech).

    Techniques: In Vivo, Functional Assay, Injection, Staining, Derivative Assay