deparaffinized sections  (Thermo Fisher)


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    Name:
    Richard Allan Scientific Tissue Section Adhesive
    Description:
    Increase the affinity of the tissue for the slide with Thermo Scientific Richard Allan Scientific Tissue Section Adhesive Pre mixed and ready to use this adhesive will not interfere with staining reactions
    Catalog Number:
    86014
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    Lab Supplies Plastics Glassware
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    Anatomical Pathology|Clinical|Microscope Slides, Glassware, & Accessories
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    Structured Review

    Thermo Fisher deparaffinized sections
    Increase the affinity of the tissue for the slide with Thermo Scientific Richard Allan Scientific Tissue Section Adhesive Pre mixed and ready to use this adhesive will not interfere with staining reactions
    https://www.bioz.com/result/deparaffinized sections/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinized sections - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Derivation and characterization of putative craniofacial mesenchymal progenitor cells from human induced pluripotent stem cells
    Article Snippet: Within 14 days, cells formed micro-masses that were stained using the Alcian Blue staining kit (Lifeline Cell Technologies), according to the manufacturer’s instructions. .. The harvested micro-masses were fixed with 4% PFA for 1 h, then embedded into liquefied Richard-Allan Scientific HistoGel Specimen Processing Gel (ThermoFisher Scientific), solidified on ice for 1 h, and finally paraffin embedded, sectioned, and stained with Alcian Blue stain. .. The stained micro-masses were visualized under light microscope for image capturing and analysis.

    Article Title: The Capacity of High-Grade Serous Ovarian Cancer Cells to Form Multicellular Structures Spontaneously along Disease Progression Correlates with Their Orthotopic Tumorigenicity in Immunosuppressed Mice
    Article Snippet: Thereafter, the structures were fixed in 4% PFA and stained with Giemsa. .. In other studies, the multicellular structures were fixed in 4% PFA and solidified with HistogelTM (Richard-Allan Scientific, Kalamazoo, MI, USA) before paraffin embedding, and followed by 5 μm sectioning before staining with H & E. .. Fluorescence Microscopy For a live/dead® viability/cytotoxicity assay, we followed a protocol we previously described in detail [ ]: multicellular structures were incubated for 45 min at room temperature (RT) and without fixation in the presence of 2 μM calcein AM (Molecular Probes, Eugene, OR, USA) and 4 μM ethidium homodimer 1 (EthD-1) (Molecular Probes).

    Article Title: Mouse Mast Cell Tryptase mMCP-6 Is a Critical Link between Adaptive and Innate Immunity in the Chronic Phase of Trichinella spiralis Infection 1
    Article Snippet: For toluidine blue staining, a working solution of 0.1% toluidine blue O (Sigma-Aldrich) in water was prepared. .. Tissue sections were stained for 30 s, rinsed in distilled water, next dehydrated in graded alcohol, and mounted with Cytoseal 60 (Richard-Allan Scientific). .. H & E staining and Congo red staining were performed as previously described ( ).

    Article Title: miR-210 Targets Iron-Sulfur Cluster Scaffold Homologue in Human Trophoblast Cell Lines
    Article Snippet: For the enhancement of iron staining signals, the cells were incubated with 0.75 mg/mL 3,3′–diaminobenzidine (Sigma–Aldrich) and 0.07% H2 O2 in 1 mol/L Tris–HCl (pH 7.5) for 5 minutes, followed by rinsing with PBS. .. For the demonstration of iron in the tissues, a Perls' reaction was performed with an iron staining kit (Richard–Allan Scientific, Kalamazoo, MI), according to the manufacturer's instructions, using formalin–fixed paraffin sections (5–μm thick). ..

    Immunofluorescence:

    Article Title: Simple 3D culture of dissociated kidney mesenchyme mimics nephron progenitor niche and facilitates nephrogenesis Wnt-independently
    Article Snippet: The recombinant tissue was cultured in Trowell type tissue culture for 72 hours, followed by fixation with 4% PFA for 1 hour at room temperature and subjected to immunofluorescence staining. .. Immunofluorescence The KM spheres were fixed for 20 min at RT with 4% PFA (unless otherwise stated), washed with PBS, pelleted in 1.5 ml low protein binding tubes, embedded in Richard-Allan Scientific HistoGel (for paraffin sections) or OCT embedding compound (for cryosections), and subsequently cut at 5 µm section in microtome or cryostat. ..

    Low Protein Binding:

    Article Title: Simple 3D culture of dissociated kidney mesenchyme mimics nephron progenitor niche and facilitates nephrogenesis Wnt-independently
    Article Snippet: The recombinant tissue was cultured in Trowell type tissue culture for 72 hours, followed by fixation with 4% PFA for 1 hour at room temperature and subjected to immunofluorescence staining. .. Immunofluorescence The KM spheres were fixed for 20 min at RT with 4% PFA (unless otherwise stated), washed with PBS, pelleted in 1.5 ml low protein binding tubes, embedded in Richard-Allan Scientific HistoGel (for paraffin sections) or OCT embedding compound (for cryosections), and subsequently cut at 5 µm section in microtome or cryostat. ..

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    Thermo Fisher anti gpx4
    Erastin promoted matrix metalloproteinase 13 (MMP13) expression while inhibited Type II collagen (collagen II) expression in chondrocytes (A) The protein expression level of <t>GPX4,</t> collagen II and MMP13 when treated with erastin were detected by western blot (B) The band density ratio of GPX4, collagen II and MMP13 to β-actin in the western blots were quantified by densitometry (C) The protein expression level of collagen II and MMP13 when treated by IL-1β with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (D-E) The band density ratio of collagen II and MMP13 to β-actin in the western blots were quantified by densitometry (F) The protein expression level of collagen II and MMP13 when treated by FAC with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (G-H) The band density ratio of collagen II and MMP13 to β-actin in the western blots were quantified by densitometry.∗P
    Anti Gpx4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gpx4/product/Thermo Fisher
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    97
    Thermo Fisher rabbit anti gfp antibody
    Detection of circulating soluble IGF-IR in mice implanted with genetically engineered marrow stromal cells. Ten million MSCs were mixed with Matrigel and implanted subcutaneously into ( a ) syngeneic C57Bl/6 or ( c ) athymic mice. The mice were bled at several intervals postimplantation, the plasma separated and soluble IGF-IR levels measured using the ELISA. To avoid daily bleeding of the same mice, the animals were separated into groups that were bled twice weekly and the data for each time point pooled to generate the curve shown. Each value represents the mean (and SD) of a minimum of three (and up to 33) individual measurements performed on the indicated days. Blood samples collected from mice implanted with mock-transduced MSC (MSC <t>GFP</t> ) or MSC producing erythropoietin (MSC EPO ) were used as controls. Shown in b are representative confocal microscopy images taken of sections prepared from formalin fixed and paraffin-embedded Matrigel plugs containing the indicated cells that were removed 22 days following subcutaneous implantation. The sections were stained with a rabbit antibody to GFP followed by an <t>Alexa</t> Fluor 568 secondary antibody and imaged using confocal microscopy with a ×40 objective. ELISA, enzyme-linked immunosorbent assay; IGF-IR, insulin-like growth factor-I receptor.
    Rabbit Anti Gfp Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gfp antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
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    95
    Thermo Fisher sytox orange
    Extracellular DNA is present in the lungs of an M. haemolytica -infected calf. Tissue sections (10 μm) were obtained from an M. haemolytica -infected calf and a healthy calf, <t>deparaffinized,</t> and incubated with <t>Sytox</t> Orange to stain DNA, or PBS as
    Sytox Orange, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sytox orange/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sytox orange - by Bioz Stars, 2021-03
    95/100 stars
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    Image Search Results


    Erastin promoted matrix metalloproteinase 13 (MMP13) expression while inhibited Type II collagen (collagen II) expression in chondrocytes (A) The protein expression level of GPX4, collagen II and MMP13 when treated with erastin were detected by western blot (B) The band density ratio of GPX4, collagen II and MMP13 to β-actin in the western blots were quantified by densitometry (C) The protein expression level of collagen II and MMP13 when treated by IL-1β with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (D-E) The band density ratio of collagen II and MMP13 to β-actin in the western blots were quantified by densitometry (F) The protein expression level of collagen II and MMP13 when treated by FAC with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (G-H) The band density ratio of collagen II and MMP13 to β-actin in the western blots were quantified by densitometry.∗P

    Journal: Journal of Orthopaedic Translation

    Article Title: Chondrocyte ferroptosis contribute to the progression of osteoarthritis

    doi: 10.1016/j.jot.2020.09.006

    Figure Lengend Snippet: Erastin promoted matrix metalloproteinase 13 (MMP13) expression while inhibited Type II collagen (collagen II) expression in chondrocytes (A) The protein expression level of GPX4, collagen II and MMP13 when treated with erastin were detected by western blot (B) The band density ratio of GPX4, collagen II and MMP13 to β-actin in the western blots were quantified by densitometry (C) The protein expression level of collagen II and MMP13 when treated by IL-1β with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (D-E) The band density ratio of collagen II and MMP13 to β-actin in the western blots were quantified by densitometry (F) The protein expression level of collagen II and MMP13 when treated by FAC with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (G-H) The band density ratio of collagen II and MMP13 to β-actin in the western blots were quantified by densitometry.∗P

    Article Snippet: Other sections were deparaffinized, antigen retrieved, incubated with anti-GPX4 or anti-Collagen II antibody, and then incubated with goat anti-rabbit secondary antibody.

    Techniques: Expressing, Western Blot

    Nrf2 antioxidant system and ferroptosis are mutually regulated under inflammation and iron overload condition (A) The protein expression level of Nrf2, HO-1, NQO-1 and Trx when treated by IL-1β with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (B) The band density ratio of Nrf2, HO-1, NQO-1 and Trx to GAPDH in the western blots were quantified by densitometry (C) The protein expression level of Nrf2, HO-1, NQO-1 and Trx when treated by FAC with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (D) The band density ratio of Nrf2, HO-1, NQO-1 and Trx to GAPDH in the western blots were quantified by densitometry. (E) The nuclear protein level of Nrf2 and HO-1 when treated by IL-1β with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (F) The band density ratio of Nrf2 and HO-1 to Lamin B were quantified by densitometry. (G) The nuclear protein level of Nrf2 and HO-1 when treated by FAC with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (H) The band density ratio of Nrf2 and HO-1 to Lamin B were quantified by densitometry. (I) The protein level of GPX4 when treated by IL-1β with si-Nrf2 or negative control were detected by western blot (J) The band density ratio of GPX4 to GAPDH were quantified by densitometry. (K) The protein level of GPX4 when treated by IL-1β with si-Nrf2 or negative control were detected by western blot (L) The band density ratio of GPX4 to GAPDH were quantified by densitometry.∗P

    Journal: Journal of Orthopaedic Translation

    Article Title: Chondrocyte ferroptosis contribute to the progression of osteoarthritis

    doi: 10.1016/j.jot.2020.09.006

    Figure Lengend Snippet: Nrf2 antioxidant system and ferroptosis are mutually regulated under inflammation and iron overload condition (A) The protein expression level of Nrf2, HO-1, NQO-1 and Trx when treated by IL-1β with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (B) The band density ratio of Nrf2, HO-1, NQO-1 and Trx to GAPDH in the western blots were quantified by densitometry (C) The protein expression level of Nrf2, HO-1, NQO-1 and Trx when treated by FAC with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (D) The band density ratio of Nrf2, HO-1, NQO-1 and Trx to GAPDH in the western blots were quantified by densitometry. (E) The nuclear protein level of Nrf2 and HO-1 when treated by IL-1β with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (F) The band density ratio of Nrf2 and HO-1 to Lamin B were quantified by densitometry. (G) The nuclear protein level of Nrf2 and HO-1 when treated by FAC with 1 μM ferrostain-1 or equal volume of DMSO were detected by western blot (H) The band density ratio of Nrf2 and HO-1 to Lamin B were quantified by densitometry. (I) The protein level of GPX4 when treated by IL-1β with si-Nrf2 or negative control were detected by western blot (J) The band density ratio of GPX4 to GAPDH were quantified by densitometry. (K) The protein level of GPX4 when treated by IL-1β with si-Nrf2 or negative control were detected by western blot (L) The band density ratio of GPX4 to GAPDH were quantified by densitometry.∗P

    Article Snippet: Other sections were deparaffinized, antigen retrieved, incubated with anti-GPX4 or anti-Collagen II antibody, and then incubated with goat anti-rabbit secondary antibody.

    Techniques: Expressing, Western Blot, Negative Control

    Ferrostatin-1 attenuated the cytotoxicity, ROS and lipid-ROS accumulation and ferroptosis related protein expression changes induced by IL-1β and FAC (A–B) Cell viability determined by CCK-8 assay (C) Intracellular ROS and lipid-ROS level detected by DCFH-DA and C11 BODIPY fluorescent probe (D) The protein expression level of ACSL4, GPX4, P53, and SLC7A11 when treated by IL-1β with 1 ​μM ferrostain-1 or equal volume of DMSO were detected by western blot (E) Band density ratios of ACSL4, GPX4, P53 and SLC7A11 to GAPDH in the western blots were quantified by densitometry (F) The protein expression level of ACSL4, GPX4, P53 and SLC7A11 when treated by FAC with 1 ​μM ferrostain-1 or equal volume of DMSO were detected by western blot (G) Band density ratios of ACSL4, GPX4, P53 and SLC7A11 to GAPDH in the western blots were quantified by densitometry. ∗P ​

    Journal: Journal of Orthopaedic Translation

    Article Title: Chondrocyte ferroptosis contribute to the progression of osteoarthritis

    doi: 10.1016/j.jot.2020.09.006

    Figure Lengend Snippet: Ferrostatin-1 attenuated the cytotoxicity, ROS and lipid-ROS accumulation and ferroptosis related protein expression changes induced by IL-1β and FAC (A–B) Cell viability determined by CCK-8 assay (C) Intracellular ROS and lipid-ROS level detected by DCFH-DA and C11 BODIPY fluorescent probe (D) The protein expression level of ACSL4, GPX4, P53, and SLC7A11 when treated by IL-1β with 1 ​μM ferrostain-1 or equal volume of DMSO were detected by western blot (E) Band density ratios of ACSL4, GPX4, P53 and SLC7A11 to GAPDH in the western blots were quantified by densitometry (F) The protein expression level of ACSL4, GPX4, P53 and SLC7A11 when treated by FAC with 1 ​μM ferrostain-1 or equal volume of DMSO were detected by western blot (G) Band density ratios of ACSL4, GPX4, P53 and SLC7A11 to GAPDH in the western blots were quantified by densitometry. ∗P ​

    Article Snippet: Other sections were deparaffinized, antigen retrieved, incubated with anti-GPX4 or anti-Collagen II antibody, and then incubated with goat anti-rabbit secondary antibody.

    Techniques: Expressing, CCK-8 Assay, Western Blot

    (A) The total GPX4 protein level in the chondrocytes treated with IL-1β or in combination with ferrostain-1 was evaluated by immunofluorescence staining. The nuclei were stained with DAPI (B) The total GPX4 protein level in the chondrocytes treated with FAC or in combination with ferrostain-1were evaluated by immunofluorescence staining. The nuclei were stained with DAPI.

    Journal: Journal of Orthopaedic Translation

    Article Title: Chondrocyte ferroptosis contribute to the progression of osteoarthritis

    doi: 10.1016/j.jot.2020.09.006

    Figure Lengend Snippet: (A) The total GPX4 protein level in the chondrocytes treated with IL-1β or in combination with ferrostain-1 was evaluated by immunofluorescence staining. The nuclei were stained with DAPI (B) The total GPX4 protein level in the chondrocytes treated with FAC or in combination with ferrostain-1were evaluated by immunofluorescence staining. The nuclei were stained with DAPI.

    Article Snippet: Other sections were deparaffinized, antigen retrieved, incubated with anti-GPX4 or anti-Collagen II antibody, and then incubated with goat anti-rabbit secondary antibody.

    Techniques: Immunofluorescence, Staining

    Both IL-1β and FAC induced ferroptosis related protein expression changes in chondrocytes (A) The protein expression level of ACSL4, GPX4, P53, and SLC7A11, when treated with IL-1β, were detected by western blot (B) Band density ratios of ACSL4, GPX4, P53, and SLC7A11 to GAPDH in the western blots were quantified by densitometry (C) The protein expression level of ACSL4, GPX4, P53, and SLC7A11 when treated with FAC were detected by western blot (D) Band density ratios of ACSL4, GPX4, P53 and SLC7A11 to GAPDH in the western blots were quantified by densitometry (E) Total GPX4 protein level were evaluated by immunofluorescence staining in chondrocytes treated with IL-1β or FAC. ∗P ​

    Journal: Journal of Orthopaedic Translation

    Article Title: Chondrocyte ferroptosis contribute to the progression of osteoarthritis

    doi: 10.1016/j.jot.2020.09.006

    Figure Lengend Snippet: Both IL-1β and FAC induced ferroptosis related protein expression changes in chondrocytes (A) The protein expression level of ACSL4, GPX4, P53, and SLC7A11, when treated with IL-1β, were detected by western blot (B) Band density ratios of ACSL4, GPX4, P53, and SLC7A11 to GAPDH in the western blots were quantified by densitometry (C) The protein expression level of ACSL4, GPX4, P53, and SLC7A11 when treated with FAC were detected by western blot (D) Band density ratios of ACSL4, GPX4, P53 and SLC7A11 to GAPDH in the western blots were quantified by densitometry (E) Total GPX4 protein level were evaluated by immunofluorescence staining in chondrocytes treated with IL-1β or FAC. ∗P ​

    Article Snippet: Other sections were deparaffinized, antigen retrieved, incubated with anti-GPX4 or anti-Collagen II antibody, and then incubated with goat anti-rabbit secondary antibody.

    Techniques: Expressing, Western Blot, Immunofluorescence, Staining

    Ferrostain-1 attenuated cartilage degradation and increased the Collagen II and GPX4 expression in the mouse OA model (A) Cartilage degradation was assessed by Safranin O/fast green staining (B) Immunohistochemistry staining of GPX4 and collagen II (C) Number of GPX4 positive cells per field under 200-time magnification (D) The number of collagen positive cells per field under 200-time magnification (E) The progression of OA was evaluated using the OARSI scores. ∗P ​

    Journal: Journal of Orthopaedic Translation

    Article Title: Chondrocyte ferroptosis contribute to the progression of osteoarthritis

    doi: 10.1016/j.jot.2020.09.006

    Figure Lengend Snippet: Ferrostain-1 attenuated cartilage degradation and increased the Collagen II and GPX4 expression in the mouse OA model (A) Cartilage degradation was assessed by Safranin O/fast green staining (B) Immunohistochemistry staining of GPX4 and collagen II (C) Number of GPX4 positive cells per field under 200-time magnification (D) The number of collagen positive cells per field under 200-time magnification (E) The progression of OA was evaluated using the OARSI scores. ∗P ​

    Article Snippet: Other sections were deparaffinized, antigen retrieved, incubated with anti-GPX4 or anti-Collagen II antibody, and then incubated with goat anti-rabbit secondary antibody.

    Techniques: Expressing, Staining, Immunohistochemistry

    Detection of circulating soluble IGF-IR in mice implanted with genetically engineered marrow stromal cells. Ten million MSCs were mixed with Matrigel and implanted subcutaneously into ( a ) syngeneic C57Bl/6 or ( c ) athymic mice. The mice were bled at several intervals postimplantation, the plasma separated and soluble IGF-IR levels measured using the ELISA. To avoid daily bleeding of the same mice, the animals were separated into groups that were bled twice weekly and the data for each time point pooled to generate the curve shown. Each value represents the mean (and SD) of a minimum of three (and up to 33) individual measurements performed on the indicated days. Blood samples collected from mice implanted with mock-transduced MSC (MSC GFP ) or MSC producing erythropoietin (MSC EPO ) were used as controls. Shown in b are representative confocal microscopy images taken of sections prepared from formalin fixed and paraffin-embedded Matrigel plugs containing the indicated cells that were removed 22 days following subcutaneous implantation. The sections were stained with a rabbit antibody to GFP followed by an Alexa Fluor 568 secondary antibody and imaged using confocal microscopy with a ×40 objective. ELISA, enzyme-linked immunosorbent assay; IGF-IR, insulin-like growth factor-I receptor.

    Journal: Molecular Therapy: the Journal of the American Society of Gene Therapy

    Article Title: Autologous Bone Marrow Stromal Cells Genetically Engineered to Secrete an IGF-I Receptor Decoy Prevent the Growth of Liver Metastases

    doi: 10.1038/mt.2009.82

    Figure Lengend Snippet: Detection of circulating soluble IGF-IR in mice implanted with genetically engineered marrow stromal cells. Ten million MSCs were mixed with Matrigel and implanted subcutaneously into ( a ) syngeneic C57Bl/6 or ( c ) athymic mice. The mice were bled at several intervals postimplantation, the plasma separated and soluble IGF-IR levels measured using the ELISA. To avoid daily bleeding of the same mice, the animals were separated into groups that were bled twice weekly and the data for each time point pooled to generate the curve shown. Each value represents the mean (and SD) of a minimum of three (and up to 33) individual measurements performed on the indicated days. Blood samples collected from mice implanted with mock-transduced MSC (MSC GFP ) or MSC producing erythropoietin (MSC EPO ) were used as controls. Shown in b are representative confocal microscopy images taken of sections prepared from formalin fixed and paraffin-embedded Matrigel plugs containing the indicated cells that were removed 22 days following subcutaneous implantation. The sections were stained with a rabbit antibody to GFP followed by an Alexa Fluor 568 secondary antibody and imaged using confocal microscopy with a ×40 objective. ELISA, enzyme-linked immunosorbent assay; IGF-IR, insulin-like growth factor-I receptor.

    Article Snippet: The sections were deparaffinized and immunostained with a rabbit anti-GFP antibody and an Alexa Fluor 568 goat anti-rabbit IgG (both from Molecular Probes, Invitrogen Canada), both used at a dilution of 1:200.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Confocal Microscopy, Staining

    Extracellular DNA is present in the lungs of an M. haemolytica -infected calf. Tissue sections (10 μm) were obtained from an M. haemolytica -infected calf and a healthy calf, deparaffinized, and incubated with Sytox Orange to stain DNA, or PBS as

    Journal: Infection and Immunity

    Article Title: Mannheimia haemolytica and Its Leukotoxin Cause Neutrophil Extracellular Trap Formation by Bovine Neutrophils ▿

    doi: 10.1128/IAI.00840-10

    Figure Lengend Snippet: Extracellular DNA is present in the lungs of an M. haemolytica -infected calf. Tissue sections (10 μm) were obtained from an M. haemolytica -infected calf and a healthy calf, deparaffinized, and incubated with Sytox Orange to stain DNA, or PBS as

    Article Snippet: Sections were deparaffinized and incubated for 1 h at room temperature with 2 μM Sytox Orange (Invitrogen), washed, and examined using a Nikon Eclipse E800 immunofluorescence microscope.

    Techniques: Infection, Incubation, Staining