deparaffinized sections  (Abcam)

 
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    Name:
    Recombinant Human KMT6 EZH2 protein
    Description:

    Catalog Number:
    ab132934
    Price:
    None
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    Structured Review

    Abcam deparaffinized sections

    https://www.bioz.com/result/deparaffinized sections/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinized sections - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Purification:

    Article Title: EZH2 contributes to the response to PARP inhibitors through its PARP-mediated poly-ADP ribosylation in breast cancer
    Article Snippet: The following antibodies were used: EZH2 (#5246, Cell Signaling Technology; 612667, BD Biosciences), EED (sc-28701, Santa Cruz Biotechnology; 09-774, EMD Millipore), SUZ12 (#3737, Cell Signaling Technology), PAR (4335-MC-100-AC, Trevigen; 551813, BD Biosciences), PARP1 (#9532, Cell Signaling Technology; sc-7150, Santa Cruz Biotechnology), HA (11666606001, Sigma-Aldrich; sc-805, Santa Cruz Biotechnology), Histone H3 (ab1791, Abcam; #3638, Cell Signaling Technology), H3-K27-me3 (ab6002, ab108245, Abcam), GST (sc-53909, Santa Cruz Biotechnology), γ-H2AX (#05-636, EMD Millipore), Tubulin (T5168, Sigma-Aldrich). .. Purified PARP protein and GST-EZH2 were purchased from Abcam and BPS Bioscience, respectively. ..

    Western Blot:

    Article Title: LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression
    Article Snippet: Then, we used GSK126, an inhibitor of EZH2 catalytic activity, to treat with SUNE-1 cells. .. Western blot results showed that histone marker H3K27me3 was significantly decreased after 10 µm GSK126 treating for 48 hours in SUNE-1 cells, without change in the protein level of EZH2 ( ). ..

    Article Title: LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression
    Article Snippet: Thus, it is believed that ANCR is required for EZH2 binding on PTEN promoter. .. It was reported that ANCR can regulate EZH2 expression and degradation., To examine this possibility in NPC cells, we used Western blot to check the protein level of EZH2 and the result showed that ANCR did not affect EZH2 expression in NPC cells ( ). .. These data indicated that EZH2 binding on PTEN promoter was ANCR dependent.

    Article Title: LINC00152 upregulates ZEB1 expression and enhances epithelial-mesenchymal transition and oxaliplatin resistance in esophageal cancer by interacting with EZH2
    Article Snippet: First, RT-qPCR results displayed that mRNA expression of EZH2 in EC tissues, Kyse-150 and TE-1 cells were notably higher than those in normal adjacent tissues and Het-1A cells (Fig. e-f; p < 0.05). .. Then, results of Western blot analysis suggested that protein expression of EZH2 in EC tissues, Kyse-150 and TE-1 cells was also notably higher than that in normal adjacent tissues and Het-1A, respectively (Fig. g-h; p< 0.05). .. Results of the CCK-8 assay suggested that, compared with cells transfected with oe-LINC00152, survival rate in cells transfected with oe-LINC00152 and si-EZH2 was notably lower after treatments of different doses of L-OHP in Kyse-150 and TE-1 cells for 72 h (p < 0.05).

    Article Title: LINC00152 upregulates ZEB1 expression and enhances epithelial-mesenchymal transition and oxaliplatin resistance in esophageal cancer by interacting with EZH2
    Article Snippet: Additionally, in comparison with the treatment of oe-LINC00152 and si-NC plus L-OHP, the treatment of oe-LINC00152 and si-ZEB1 plus L-OHP led to decreased tumor growth rate, indicating that silencing ZEB1 could inhibit the resistance in EC induced by LINC00152 overexpression (Fig. a) (p< 0.05). .. RT-qPCR was conducted to measure the relative expression of LINC00152, EZH2, and ZEB1 while Western blot analysis was carried out to detect the protein expression of EZH2, and ZEB1. .. The results showed that the expression of LINC00152, EZH2, and ZEB1 was elevated in the tumors of mice treated with oe-LINC00152 and si-NC plus L-OHP versus the mice treated with oe-NC and si-NC plus L-OHP.

    Article Title: MiR-26a inhibits cell proliferation and induces apoptosis in human bladder cancer through regulating EZH2 bioactivity
    Article Snippet: Mimic-mi26a and siRNA was purchased in GenePharma Inc. .. The antibody of EZH2 protein used in western blot and immunohistochemistry experiments is Rabbit-anti-EZH2 (abcam). .. Total RNA was extracted using Trizol (TAKARA) and cDNA synthesis using Superscript II reverse transcriptase (DBI) was primed with random hexamer primers following the manufacturer’s protocols [ ].

    Marker:

    Article Title: LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression
    Article Snippet: Then, we used GSK126, an inhibitor of EZH2 catalytic activity, to treat with SUNE-1 cells. .. Western blot results showed that histone marker H3K27me3 was significantly decreased after 10 µm GSK126 treating for 48 hours in SUNE-1 cells, without change in the protein level of EZH2 ( ). ..

    Expressing:

    Article Title: LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression
    Article Snippet: Thus, it is believed that ANCR is required for EZH2 binding on PTEN promoter. .. It was reported that ANCR can regulate EZH2 expression and degradation., To examine this possibility in NPC cells, we used Western blot to check the protein level of EZH2 and the result showed that ANCR did not affect EZH2 expression in NPC cells ( ). .. These data indicated that EZH2 binding on PTEN promoter was ANCR dependent.

    Article Title: LINC00152 upregulates ZEB1 expression and enhances epithelial-mesenchymal transition and oxaliplatin resistance in esophageal cancer by interacting with EZH2
    Article Snippet: First, RT-qPCR results displayed that mRNA expression of EZH2 in EC tissues, Kyse-150 and TE-1 cells were notably higher than those in normal adjacent tissues and Het-1A cells (Fig. e-f; p < 0.05). .. Then, results of Western blot analysis suggested that protein expression of EZH2 in EC tissues, Kyse-150 and TE-1 cells was also notably higher than that in normal adjacent tissues and Het-1A, respectively (Fig. g-h; p< 0.05). .. Results of the CCK-8 assay suggested that, compared with cells transfected with oe-LINC00152, survival rate in cells transfected with oe-LINC00152 and si-EZH2 was notably lower after treatments of different doses of L-OHP in Kyse-150 and TE-1 cells for 72 h (p < 0.05).

    Article Title: LINC00152 upregulates ZEB1 expression and enhances epithelial-mesenchymal transition and oxaliplatin resistance in esophageal cancer by interacting with EZH2
    Article Snippet: Additionally, in comparison with the treatment of oe-LINC00152 and si-NC plus L-OHP, the treatment of oe-LINC00152 and si-ZEB1 plus L-OHP led to decreased tumor growth rate, indicating that silencing ZEB1 could inhibit the resistance in EC induced by LINC00152 overexpression (Fig. a) (p< 0.05). .. RT-qPCR was conducted to measure the relative expression of LINC00152, EZH2, and ZEB1 while Western blot analysis was carried out to detect the protein expression of EZH2, and ZEB1. .. The results showed that the expression of LINC00152, EZH2, and ZEB1 was elevated in the tumors of mice treated with oe-LINC00152 and si-NC plus L-OHP versus the mice treated with oe-NC and si-NC plus L-OHP.

    Magnetic Beads:

    Article Title: Regulation of Viral and Cellular Gene Expression by Kaposi's Sarcoma-Associated Herpesvirus Polyadenylated Nuclear RNA
    Article Snippet: Cells were pelleted, washed once with PBS, and quenched with 125 mM glycine for 5 min. After a final wash with PBS, cells were placed in in 2 ml RIPA buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 2 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) with protease inhibitor cocktail (Sigma), RNase Out (Invitrogen), and 1 mM phenylmethylsulfonyl fluoride (PMSF). .. RNA was precipitated by adding 300 μl lysate, 5 μl antibody to SUZ12 and EZH2 (Abcam), 50 μl protein G magnetic beads (Active Motif), and 1 μl RNase Out. ..

    Quantitative RT-PCR:

    Article Title: LINC00152 upregulates ZEB1 expression and enhances epithelial-mesenchymal transition and oxaliplatin resistance in esophageal cancer by interacting with EZH2
    Article Snippet: Additionally, in comparison with the treatment of oe-LINC00152 and si-NC plus L-OHP, the treatment of oe-LINC00152 and si-ZEB1 plus L-OHP led to decreased tumor growth rate, indicating that silencing ZEB1 could inhibit the resistance in EC induced by LINC00152 overexpression (Fig. a) (p< 0.05). .. RT-qPCR was conducted to measure the relative expression of LINC00152, EZH2, and ZEB1 while Western blot analysis was carried out to detect the protein expression of EZH2, and ZEB1. .. The results showed that the expression of LINC00152, EZH2, and ZEB1 was elevated in the tumors of mice treated with oe-LINC00152 and si-NC plus L-OHP versus the mice treated with oe-NC and si-NC plus L-OHP.

    Immunohistochemistry:

    Article Title: MiR-26a inhibits cell proliferation and induces apoptosis in human bladder cancer through regulating EZH2 bioactivity
    Article Snippet: Mimic-mi26a and siRNA was purchased in GenePharma Inc. .. The antibody of EZH2 protein used in western blot and immunohistochemistry experiments is Rabbit-anti-EZH2 (abcam). .. Total RNA was extracted using Trizol (TAKARA) and cDNA synthesis using Superscript II reverse transcriptase (DBI) was primed with random hexamer primers following the manufacturer’s protocols [ ].

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    Abcam mouse monoclonal antibodies against progerin
    Increased <t>progerin</t> expression and TUNEL+ cells in DCM hearts. A Immunohistochemistry showing increased numbers of progerin expressing cells (arrows, red nuclei) in DCM hearts compared to non-failing controls. Scale bar represents 100μM (rows 1–2) and 25μM (rows 3–4). B Immunofluorescence stainings revealed increased expression of progerin (green, arrows) in nuclei (blue) of DCM hearts (merged images last row, arrows) compared to non-failing controls. Red: WGA membrane staining, Green: Progerin staining, Blue: DAPI+ nuclei. Scale bar represents 20μM. C TUNEL staining of apoptotic cell death revealed increased numbers of TUNEL+ cells in DCM hearts compared to non-failing controls. Scale bar represents 200μM (rows 1–3) and 20μM (last row). D Quantification of progerin+ nuclei to total nuclei per high power field (HPF) in DCM hearts and Controls (n = 3). ** P ≤ 0.01 DCM vs. Control. E Quantification of apoptotic index (TUNEL+ nuclei to total nuclei) in DCM hearts compared to controls (n = 3). ** P ≤ 0.01 DCM vs. Control.
    Mouse Monoclonal Antibodies Against Progerin, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam mouse anti human cd147
    Changes in MUC5AC after <t>CD147</t> silencing by siRNA transfection. CD147 protein ( a ) and mRNA ( b ) expression levels were assessed after siRNA transfection. Three CD147 siRNA were designed, and CD147 siRNA 1 was the most effective at silencing CD147 expression. * p
    Mouse Anti Human Cd147, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti cxcl1
    HSCs-specific deletion of CD147 deregulated <t>CXCL1</t> expression. ( A ) Fluorescence activated cell sorting (FACS) analysis of CXCL1 expression in primary HSCs isolated from Bsg fl/fl and GFAP-Cre;Bsg fl/fl mice ( n = 3); ( B ) Enzyme-linked immunosorbent assay (ELISA) detection of serum CXCL1 in CCl 4 -induced Bsg fl/fl and GFAP-Cre;Bsg fl/fl mice (eight weeks, n = 4). The results were shown as the mean ± SD. * p
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    Image Search Results


    Increased progerin expression and TUNEL+ cells in DCM hearts. A Immunohistochemistry showing increased numbers of progerin expressing cells (arrows, red nuclei) in DCM hearts compared to non-failing controls. Scale bar represents 100μM (rows 1–2) and 25μM (rows 3–4). B Immunofluorescence stainings revealed increased expression of progerin (green, arrows) in nuclei (blue) of DCM hearts (merged images last row, arrows) compared to non-failing controls. Red: WGA membrane staining, Green: Progerin staining, Blue: DAPI+ nuclei. Scale bar represents 20μM. C TUNEL staining of apoptotic cell death revealed increased numbers of TUNEL+ cells in DCM hearts compared to non-failing controls. Scale bar represents 200μM (rows 1–3) and 20μM (last row). D Quantification of progerin+ nuclei to total nuclei per high power field (HPF) in DCM hearts and Controls (n = 3). ** P ≤ 0.01 DCM vs. Control. E Quantification of apoptotic index (TUNEL+ nuclei to total nuclei) in DCM hearts compared to controls (n = 3). ** P ≤ 0.01 DCM vs. Control.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: Increased progerin expression and TUNEL+ cells in DCM hearts. A Immunohistochemistry showing increased numbers of progerin expressing cells (arrows, red nuclei) in DCM hearts compared to non-failing controls. Scale bar represents 100μM (rows 1–2) and 25μM (rows 3–4). B Immunofluorescence stainings revealed increased expression of progerin (green, arrows) in nuclei (blue) of DCM hearts (merged images last row, arrows) compared to non-failing controls. Red: WGA membrane staining, Green: Progerin staining, Blue: DAPI+ nuclei. Scale bar represents 20μM. C TUNEL staining of apoptotic cell death revealed increased numbers of TUNEL+ cells in DCM hearts compared to non-failing controls. Scale bar represents 200μM (rows 1–3) and 20μM (last row). D Quantification of progerin+ nuclei to total nuclei per high power field (HPF) in DCM hearts and Controls (n = 3). ** P ≤ 0.01 DCM vs. Control. E Quantification of apoptotic index (TUNEL+ nuclei to total nuclei) in DCM hearts compared to controls (n = 3). ** P ≤ 0.01 DCM vs. Control.

    Article Snippet: For Immunofluorescence staining, deparaffinized sections were incubated with primary mouse monoclonal antibodies against progerin (ab66587, Abcam) or (sc-81611, Santa Cruz) overnight at 4°C followed by a one-hour incubation with secondary antibody at room temperature (Alexa Fluor® 488, Molecular Probes Inc, Eugene, OR) and wheat germ agglutinin (WGA) Texas Red™-X Conjugate (W21405, Invitrogen).

    Techniques: Expressing, TUNEL Assay, Immunohistochemistry, Immunofluorescence, Whole Genome Amplification, Staining

    Progerin mRNA is upregulated in DCM hearts and is significantly correlated with measures of heart failure but not with age. A Relative amount of progerin mRNA levels related to the reference gene RPL32 in heart biopsies of patients with DCM (n = 15) and non-failing control hearts (n = 10). Shown are all individual data points, lines show mean ± SD. ** P ≤ 0.01 DCM vs. Control. B Relative amount of progerin mRNA levels related to the reference gene RPL32 in whole blood cells derived from patients with DCM (n = 56) and healthy controls (n = 10). Shown are all individual data points, lines show mean ± SD. P-value was derived from unpaired two-sided T-Test between the groups. C Scatter plot showing the negative correlation between the relative amount of progerin mRNA related to RPL32 with ejection fraction in human heart biopsies (n = 25). ** P ≤ 0.01. D Scatter plot showing the positive correlation between the relative amount of progerin mRNA related to RPL32 with left ventricular enddiastolic diameter (LVEDD) and E Scatter plot showing the positive correlation between progerin mRNA related to RPL32 with the age of the hearts in human heart biopsies (n = 25). N.s. not significant. ** P ≤ 0.01.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: Progerin mRNA is upregulated in DCM hearts and is significantly correlated with measures of heart failure but not with age. A Relative amount of progerin mRNA levels related to the reference gene RPL32 in heart biopsies of patients with DCM (n = 15) and non-failing control hearts (n = 10). Shown are all individual data points, lines show mean ± SD. ** P ≤ 0.01 DCM vs. Control. B Relative amount of progerin mRNA levels related to the reference gene RPL32 in whole blood cells derived from patients with DCM (n = 56) and healthy controls (n = 10). Shown are all individual data points, lines show mean ± SD. P-value was derived from unpaired two-sided T-Test between the groups. C Scatter plot showing the negative correlation between the relative amount of progerin mRNA related to RPL32 with ejection fraction in human heart biopsies (n = 25). ** P ≤ 0.01. D Scatter plot showing the positive correlation between the relative amount of progerin mRNA related to RPL32 with left ventricular enddiastolic diameter (LVEDD) and E Scatter plot showing the positive correlation between progerin mRNA related to RPL32 with the age of the hearts in human heart biopsies (n = 25). N.s. not significant. ** P ≤ 0.01.

    Article Snippet: For Immunofluorescence staining, deparaffinized sections were incubated with primary mouse monoclonal antibodies against progerin (ab66587, Abcam) or (sc-81611, Santa Cruz) overnight at 4°C followed by a one-hour incubation with secondary antibody at room temperature (Alexa Fluor® 488, Molecular Probes Inc, Eugene, OR) and wheat germ agglutinin (WGA) Texas Red™-X Conjugate (W21405, Invitrogen).

    Techniques: Derivative Assay

    PCR strategy for the detection of progerin expression in heart and blood. A Schematic overview of PCR based strategy to analyze progerin mRNA expression in human heart and the blood samples. Shown are the consensus donor splice sequence at the end of exon 11, the sequence of the normal LMNA cryptic splice site, and two common known mutated cryptic splice sites in HGPS patients. B First row: PCR gel showing total lamin A (first arrow) and small amounts of progerin expression (arrow showing Δ150bp band). Second row: Specific progerin expression utilizing primers spanning exon 11 to 12. Third row: RPL32 expression was used for relative quantification of progerin expression. G: Genomic DNA (G) did not reveal any significant LMNA bands in the gel verifying specific mRNA expression.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: PCR strategy for the detection of progerin expression in heart and blood. A Schematic overview of PCR based strategy to analyze progerin mRNA expression in human heart and the blood samples. Shown are the consensus donor splice sequence at the end of exon 11, the sequence of the normal LMNA cryptic splice site, and two common known mutated cryptic splice sites in HGPS patients. B First row: PCR gel showing total lamin A (first arrow) and small amounts of progerin expression (arrow showing Δ150bp band). Second row: Specific progerin expression utilizing primers spanning exon 11 to 12. Third row: RPL32 expression was used for relative quantification of progerin expression. G: Genomic DNA (G) did not reveal any significant LMNA bands in the gel verifying specific mRNA expression.

    Article Snippet: For Immunofluorescence staining, deparaffinized sections were incubated with primary mouse monoclonal antibodies against progerin (ab66587, Abcam) or (sc-81611, Santa Cruz) overnight at 4°C followed by a one-hour incubation with secondary antibody at room temperature (Alexa Fluor® 488, Molecular Probes Inc, Eugene, OR) and wheat germ agglutinin (WGA) Texas Red™-X Conjugate (W21405, Invitrogen).

    Techniques: Polymerase Chain Reaction, Expressing, Sequencing

    Co-expression of apoptotic marker caspase-3 and progerin in DCM. Immunofluorescence stainings revealed expression of the apoptotic cell death marker cleaved caspase-3 (red, arrow) and progerin (green, arrow) in the nucleus (blue) of a cardiomyocyte delineated by red cell membrane staining with WGA (merged image last row, arrow) compared to non-failing controls. Scale bar represents 20μM.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: Co-expression of apoptotic marker caspase-3 and progerin in DCM. Immunofluorescence stainings revealed expression of the apoptotic cell death marker cleaved caspase-3 (red, arrow) and progerin (green, arrow) in the nucleus (blue) of a cardiomyocyte delineated by red cell membrane staining with WGA (merged image last row, arrow) compared to non-failing controls. Scale bar represents 20μM.

    Article Snippet: For Immunofluorescence staining, deparaffinized sections were incubated with primary mouse monoclonal antibodies against progerin (ab66587, Abcam) or (sc-81611, Santa Cruz) overnight at 4°C followed by a one-hour incubation with secondary antibody at room temperature (Alexa Fluor® 488, Molecular Probes Inc, Eugene, OR) and wheat germ agglutinin (WGA) Texas Red™-X Conjugate (W21405, Invitrogen).

    Techniques: Expressing, Marker, Immunofluorescence, Staining, Whole Genome Amplification

    Alignment of progerin PCR product cDNA confirms alternative splicing in the heart. The sequence of purified progerin PCR cDNA (red letters) was aligned to the genomic sequence of LMNA exon 11 (green) to exon 12 (yellow) with the 3´UTR sequence (blue) showing the expected gap of 150bp between exon 11 to exon 12 confirming alternative splicing of progerin.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: Alignment of progerin PCR product cDNA confirms alternative splicing in the heart. The sequence of purified progerin PCR cDNA (red letters) was aligned to the genomic sequence of LMNA exon 11 (green) to exon 12 (yellow) with the 3´UTR sequence (blue) showing the expected gap of 150bp between exon 11 to exon 12 confirming alternative splicing of progerin.

    Article Snippet: For Immunofluorescence staining, deparaffinized sections were incubated with primary mouse monoclonal antibodies against progerin (ab66587, Abcam) or (sc-81611, Santa Cruz) overnight at 4°C followed by a one-hour incubation with secondary antibody at room temperature (Alexa Fluor® 488, Molecular Probes Inc, Eugene, OR) and wheat germ agglutinin (WGA) Texas Red™-X Conjugate (W21405, Invitrogen).

    Techniques: Polymerase Chain Reaction, Sequencing, Purification

    Alignment of progerin PCR product cDNA confirms alternative splicing in the heart. The sequence of purified progerin PCR cDNA (red letters) was aligned to the genomic sequence of LMNA exon 11 (green) to exon 12 (yellow) with the 3´UTR sequence (blue) showing the expected gap of 150bp between exon 11 to exon 12 confirming alternative splicing of progerin.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: Alignment of progerin PCR product cDNA confirms alternative splicing in the heart. The sequence of purified progerin PCR cDNA (red letters) was aligned to the genomic sequence of LMNA exon 11 (green) to exon 12 (yellow) with the 3´UTR sequence (blue) showing the expected gap of 150bp between exon 11 to exon 12 confirming alternative splicing of progerin.

    Article Snippet: For Immunohistochemistry staining, deparaffinized sections were incubated with progerin antibody (ab66587, Abcam) followed by Vectastain Elite ABC kit (AK-5000, Vector laboratories, Cambridgeshire, UK) and Vector® Red subtrate kit (SK-5100, Vector laboratories, Cambridgeshire, UK) staining.

    Techniques: Polymerase Chain Reaction, Sequencing, Purification

    Co-expression of apoptotic marker caspase-3 and progerin in DCM. Immunofluorescence stainings revealed expression of the apoptotic cell death marker cleaved caspase-3 (red, arrow) and progerin (green, arrow) in the nucleus (blue) of a cardiomyocyte delineated by red cell membrane staining with WGA (merged image last row, arrow) compared to non-failing controls. Scale bar represents 20μM.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: Co-expression of apoptotic marker caspase-3 and progerin in DCM. Immunofluorescence stainings revealed expression of the apoptotic cell death marker cleaved caspase-3 (red, arrow) and progerin (green, arrow) in the nucleus (blue) of a cardiomyocyte delineated by red cell membrane staining with WGA (merged image last row, arrow) compared to non-failing controls. Scale bar represents 20μM.

    Article Snippet: For Immunohistochemistry staining, deparaffinized sections were incubated with progerin antibody (ab66587, Abcam) followed by Vectastain Elite ABC kit (AK-5000, Vector laboratories, Cambridgeshire, UK) and Vector® Red subtrate kit (SK-5100, Vector laboratories, Cambridgeshire, UK) staining.

    Techniques: Expressing, Marker, Immunofluorescence, Staining, Whole Genome Amplification

    PCR strategy for the detection of progerin expression in heart and blood. A Schematic overview of PCR based strategy to analyze progerin mRNA expression in human heart and the blood samples. Shown are the consensus donor splice sequence at the end of exon 11, the sequence of the normal LMNA cryptic splice site, and two common known mutated cryptic splice sites in HGPS patients. B First row: PCR gel showing total lamin A (first arrow) and small amounts of progerin expression (arrow showing Δ150bp band). Second row: Specific progerin expression utilizing primers spanning exon 11 to 12. Third row: RPL32 expression was used for relative quantification of progerin expression. G: Genomic DNA (G) did not reveal any significant LMNA bands in the gel verifying specific mRNA expression.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: PCR strategy for the detection of progerin expression in heart and blood. A Schematic overview of PCR based strategy to analyze progerin mRNA expression in human heart and the blood samples. Shown are the consensus donor splice sequence at the end of exon 11, the sequence of the normal LMNA cryptic splice site, and two common known mutated cryptic splice sites in HGPS patients. B First row: PCR gel showing total lamin A (first arrow) and small amounts of progerin expression (arrow showing Δ150bp band). Second row: Specific progerin expression utilizing primers spanning exon 11 to 12. Third row: RPL32 expression was used for relative quantification of progerin expression. G: Genomic DNA (G) did not reveal any significant LMNA bands in the gel verifying specific mRNA expression.

    Article Snippet: For Immunohistochemistry staining, deparaffinized sections were incubated with progerin antibody (ab66587, Abcam) followed by Vectastain Elite ABC kit (AK-5000, Vector laboratories, Cambridgeshire, UK) and Vector® Red subtrate kit (SK-5100, Vector laboratories, Cambridgeshire, UK) staining.

    Techniques: Polymerase Chain Reaction, Expressing, Sequencing

    Increased progerin expression and TUNEL+ cells in DCM hearts. A Immunohistochemistry showing increased numbers of progerin expressing cells (arrows, red nuclei) in DCM hearts compared to non-failing controls. Scale bar represents 100μM (rows 1–2) and 25μM (rows 3–4). B Immunofluorescence stainings revealed increased expression of progerin (green, arrows) in nuclei (blue) of DCM hearts (merged images last row, arrows) compared to non-failing controls. Red: WGA membrane staining, Green: Progerin staining, Blue: DAPI+ nuclei. Scale bar represents 20μM. C TUNEL staining of apoptotic cell death revealed increased numbers of TUNEL+ cells in DCM hearts compared to non-failing controls. Scale bar represents 200μM (rows 1–3) and 20μM (last row). D Quantification of progerin+ nuclei to total nuclei per high power field (HPF) in DCM hearts and Controls (n = 3). ** P ≤ 0.01 DCM vs. Control. E Quantification of apoptotic index (TUNEL+ nuclei to total nuclei) in DCM hearts compared to controls (n = 3). ** P ≤ 0.01 DCM vs. Control.

    Journal: PLoS ONE

    Article Title: Upregulation of the aging related LMNA splice variant progerin in dilated cardiomyopathy

    doi: 10.1371/journal.pone.0196739

    Figure Lengend Snippet: Increased progerin expression and TUNEL+ cells in DCM hearts. A Immunohistochemistry showing increased numbers of progerin expressing cells (arrows, red nuclei) in DCM hearts compared to non-failing controls. Scale bar represents 100μM (rows 1–2) and 25μM (rows 3–4). B Immunofluorescence stainings revealed increased expression of progerin (green, arrows) in nuclei (blue) of DCM hearts (merged images last row, arrows) compared to non-failing controls. Red: WGA membrane staining, Green: Progerin staining, Blue: DAPI+ nuclei. Scale bar represents 20μM. C TUNEL staining of apoptotic cell death revealed increased numbers of TUNEL+ cells in DCM hearts compared to non-failing controls. Scale bar represents 200μM (rows 1–3) and 20μM (last row). D Quantification of progerin+ nuclei to total nuclei per high power field (HPF) in DCM hearts and Controls (n = 3). ** P ≤ 0.01 DCM vs. Control. E Quantification of apoptotic index (TUNEL+ nuclei to total nuclei) in DCM hearts compared to controls (n = 3). ** P ≤ 0.01 DCM vs. Control.

    Article Snippet: For Immunohistochemistry staining, deparaffinized sections were incubated with progerin antibody (ab66587, Abcam) followed by Vectastain Elite ABC kit (AK-5000, Vector laboratories, Cambridgeshire, UK) and Vector® Red subtrate kit (SK-5100, Vector laboratories, Cambridgeshire, UK) staining.

    Techniques: Expressing, TUNEL Assay, Immunohistochemistry, Immunofluorescence, Whole Genome Amplification, Staining

    Changes in MUC5AC after CD147 silencing by siRNA transfection. CD147 protein ( a ) and mRNA ( b ) expression levels were assessed after siRNA transfection. Three CD147 siRNA were designed, and CD147 siRNA 1 was the most effective at silencing CD147 expression. * p

    Journal: BMC Pulmonary Medicine

    Article Title: CD147 increases mucus secretion induced by cigarette smoke in COPD

    doi: 10.1186/s12890-019-0791-0

    Figure Lengend Snippet: Changes in MUC5AC after CD147 silencing by siRNA transfection. CD147 protein ( a ) and mRNA ( b ) expression levels were assessed after siRNA transfection. Three CD147 siRNA were designed, and CD147 siRNA 1 was the most effective at silencing CD147 expression. * p

    Article Snippet: The tissue sections were deparaffinized and stained with mouse anti-human CD147 (1:100, Abcam) and mouse anti-human MUC5AC (1:200, Abcam) primary antibodies overnight at 4 °C; then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (1:1000, Abcam), and the color was developed using diaminobenzidine.

    Techniques: Transfection, Expressing

    CD147 regulates the p38 MAPK/MMP9 signal pathway and is involved in CS-induced MUC5AC secretion in HBE cells. Cells were transfected with CD147 siRNA and pretreated with 10 μM SB203580 (p38 MAPK inhibitor) or 400 nM SB-3CT (MMP9 inhibitor) for 1 h; then, the cells were stimulated with 10% CS. After 24 h, MUC5AC protein ( a ) and mRNA ( b ) levels were detected. * p

    Journal: BMC Pulmonary Medicine

    Article Title: CD147 increases mucus secretion induced by cigarette smoke in COPD

    doi: 10.1186/s12890-019-0791-0

    Figure Lengend Snippet: CD147 regulates the p38 MAPK/MMP9 signal pathway and is involved in CS-induced MUC5AC secretion in HBE cells. Cells were transfected with CD147 siRNA and pretreated with 10 μM SB203580 (p38 MAPK inhibitor) or 400 nM SB-3CT (MMP9 inhibitor) for 1 h; then, the cells were stimulated with 10% CS. After 24 h, MUC5AC protein ( a ) and mRNA ( b ) levels were detected. * p

    Article Snippet: The tissue sections were deparaffinized and stained with mouse anti-human CD147 (1:100, Abcam) and mouse anti-human MUC5AC (1:200, Abcam) primary antibodies overnight at 4 °C; then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (1:1000, Abcam), and the color was developed using diaminobenzidine.

    Techniques: Transfection

    CD147 and MUC5AC expression in airways in the lung specimens. Photomicrographs of brown staining show the expression of CD147 in non-smokers ( a ), smokers without COPD ( b ) and smokers with COPD ( c ). Photomicrographs of brown staining show MUC5AC secretion in non-smokers ( d ), smokers without COPD ( e ) and smokers with COPD ( f ). Scale bars = 50 μm. Integrated optical density (IOD)/area represents the expression of CD147 ( g ) and MUC5AC ( h ). # p

    Journal: BMC Pulmonary Medicine

    Article Title: CD147 increases mucus secretion induced by cigarette smoke in COPD

    doi: 10.1186/s12890-019-0791-0

    Figure Lengend Snippet: CD147 and MUC5AC expression in airways in the lung specimens. Photomicrographs of brown staining show the expression of CD147 in non-smokers ( a ), smokers without COPD ( b ) and smokers with COPD ( c ). Photomicrographs of brown staining show MUC5AC secretion in non-smokers ( d ), smokers without COPD ( e ) and smokers with COPD ( f ). Scale bars = 50 μm. Integrated optical density (IOD)/area represents the expression of CD147 ( g ) and MUC5AC ( h ). # p

    Article Snippet: The tissue sections were deparaffinized and stained with mouse anti-human CD147 (1:100, Abcam) and mouse anti-human MUC5AC (1:200, Abcam) primary antibodies overnight at 4 °C; then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (1:1000, Abcam), and the color was developed using diaminobenzidine.

    Techniques: Expressing, Staining

    Expression of MUC5AC and CD147 induced by CS extract in HBE cells. HBE cells were treated with various concentration of CS extract (from 5 to 15%) for 24 h. MUC5AC protein ( a ) and mRNA ( b ) levels were detected by ELISA and RT-PCR, respectively. CD147 protein ( c ) and mRNA ( d ) levels were assessed by Western blotting and RT-PCR, respectively. * p

    Journal: BMC Pulmonary Medicine

    Article Title: CD147 increases mucus secretion induced by cigarette smoke in COPD

    doi: 10.1186/s12890-019-0791-0

    Figure Lengend Snippet: Expression of MUC5AC and CD147 induced by CS extract in HBE cells. HBE cells were treated with various concentration of CS extract (from 5 to 15%) for 24 h. MUC5AC protein ( a ) and mRNA ( b ) levels were detected by ELISA and RT-PCR, respectively. CD147 protein ( c ) and mRNA ( d ) levels were assessed by Western blotting and RT-PCR, respectively. * p

    Article Snippet: The tissue sections were deparaffinized and stained with mouse anti-human CD147 (1:100, Abcam) and mouse anti-human MUC5AC (1:200, Abcam) primary antibodies overnight at 4 °C; then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (1:1000, Abcam), and the color was developed using diaminobenzidine.

    Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot

    HSCs-specific deletion of CD147 deregulated CXCL1 expression. ( A ) Fluorescence activated cell sorting (FACS) analysis of CXCL1 expression in primary HSCs isolated from Bsg fl/fl and GFAP-Cre;Bsg fl/fl mice ( n = 3); ( B ) Enzyme-linked immunosorbent assay (ELISA) detection of serum CXCL1 in CCl 4 -induced Bsg fl/fl and GFAP-Cre;Bsg fl/fl mice (eight weeks, n = 4). The results were shown as the mean ± SD. * p

    Journal: International Journal of Molecular Sciences

    Article Title: CD147 Promotes CXCL1 Expression and Modulates Liver Fibrogenesis

    doi: 10.3390/ijms19041145

    Figure Lengend Snippet: HSCs-specific deletion of CD147 deregulated CXCL1 expression. ( A ) Fluorescence activated cell sorting (FACS) analysis of CXCL1 expression in primary HSCs isolated from Bsg fl/fl and GFAP-Cre;Bsg fl/fl mice ( n = 3); ( B ) Enzyme-linked immunosorbent assay (ELISA) detection of serum CXCL1 in CCl 4 -induced Bsg fl/fl and GFAP-Cre;Bsg fl/fl mice (eight weeks, n = 4). The results were shown as the mean ± SD. * p

    Article Snippet: Sections were deparaffinized and incubated with primary antibodies including anti-CXCL1 (1:100; ab86436), anti-α-SMA (1:100; ab7817), and anti-desmin (1:100; ab86592) (Abcam, Cambridge, UK).

    Techniques: Expressing, Fluorescence, FACS, Isolation, Mouse Assay, Enzyme-linked Immunosorbent Assay

    CXCL1 promoted HSCs activation and co-localized with CD147 in HSCs. ( A ) Flow cytometry analyses of α-SMA expression; ( B ) Real-time RT-PCR detection of α1(I) collagen mRNA level. GAPDH was used as the normalization control; ( C ) Representative phase contrast images and quantitative analysis of collagen-based cell contraction; ( D ) CCK-8 assay; ( E ) Immunofluorescence detection of CD147, CXCL1 and α-SMA in liver tissues from normal control and CCl 4 -induced mice (eight weeks, n = 5). Arrows indicate α-SMA (blue), CD147 (green), and CXCL1 (red) expression in the activated HSCs. LX-2 cells were treated with 100 ng/mL rCXCL1 for 24 h. The results were shown as the mean ± SD. * p

    Journal: International Journal of Molecular Sciences

    Article Title: CD147 Promotes CXCL1 Expression and Modulates Liver Fibrogenesis

    doi: 10.3390/ijms19041145

    Figure Lengend Snippet: CXCL1 promoted HSCs activation and co-localized with CD147 in HSCs. ( A ) Flow cytometry analyses of α-SMA expression; ( B ) Real-time RT-PCR detection of α1(I) collagen mRNA level. GAPDH was used as the normalization control; ( C ) Representative phase contrast images and quantitative analysis of collagen-based cell contraction; ( D ) CCK-8 assay; ( E ) Immunofluorescence detection of CD147, CXCL1 and α-SMA in liver tissues from normal control and CCl 4 -induced mice (eight weeks, n = 5). Arrows indicate α-SMA (blue), CD147 (green), and CXCL1 (red) expression in the activated HSCs. LX-2 cells were treated with 100 ng/mL rCXCL1 for 24 h. The results were shown as the mean ± SD. * p

    Article Snippet: Sections were deparaffinized and incubated with primary antibodies including anti-CXCL1 (1:100; ab86436), anti-α-SMA (1:100; ab7817), and anti-desmin (1:100; ab86592) (Abcam, Cambridge, UK).

    Techniques: Activation Assay, Flow Cytometry, Cytometry, Expressing, Quantitative RT-PCR, CCK-8 Assay, Immunofluorescence, Mouse Assay

    CXCL1 expression increased in liver fibrosis. ( A ) Real-time RT-PCR detection of hepatic gene expression of CXCL1 in normal control and carbon tetrachloride (CCl 4 )-treated mice ( n = 5). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the normalization control, ** p

    Journal: International Journal of Molecular Sciences

    Article Title: CD147 Promotes CXCL1 Expression and Modulates Liver Fibrogenesis

    doi: 10.3390/ijms19041145

    Figure Lengend Snippet: CXCL1 expression increased in liver fibrosis. ( A ) Real-time RT-PCR detection of hepatic gene expression of CXCL1 in normal control and carbon tetrachloride (CCl 4 )-treated mice ( n = 5). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the normalization control, ** p

    Article Snippet: Sections were deparaffinized and incubated with primary antibodies including anti-CXCL1 (1:100; ab86436), anti-α-SMA (1:100; ab7817), and anti-desmin (1:100; ab86592) (Abcam, Cambridge, UK).

    Techniques: Expressing, Quantitative RT-PCR, Mouse Assay