deparaffinization  (Thermo Fisher)


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    Name:
    Lab Vision PT Module Deparaffinization and Heat Induced Epitope Retrieval Solutions 100X
    Description:
    Perform Heat Induced Antigen Retrieval HIER on formalin fixed paraffin embedded tissue sections mounted on glass microscope slides with Thermo Scientific Lab Vision PT Module Deparaffinization and Heat Induced Epitope Retrieval Solutions
    Catalog Number:
    ta-125-pm1x
    Price:
    None
    Category:
    Lab Reagents and Chemicals
    Applications:
    Anatomical Pathology|Clinical
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    Structured Review

    Thermo Fisher deparaffinization
    Perform Heat Induced Antigen Retrieval HIER on formalin fixed paraffin embedded tissue sections mounted on glass microscope slides with Thermo Scientific Lab Vision PT Module Deparaffinization and Heat Induced Epitope Retrieval Solutions
    https://www.bioz.com/result/deparaffinization/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinization - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Activity of the GHRH Antagonist MIA602 and its Underlying Mechanisms of Action in sarcoidosis
    Article Snippet: Confocal immunofluorescence images were acquired using a Leica DM6000 microscope with a SP5 confocal module at the University of Miami McKnight Analytical Imaging Core Facility.Captured images were processed using Velocity Software version 6.1.1 software (Perkin-Elmer, Waltham, MA). .. For immunohistochemistry,5-μm paraffin sections were processed by deparaffinization and rehydration followed by endogenous peroxidase blocking (1% H2 O2 in methanol for 20 minutes) and antigen retrieval (boiled in 10 mM citrate buffer for 30 minutes). .. Tissue sections were blocked with 2% goat or horse serum (Vector Laboratories) and incubated with antibody CD68 (Proteintech, Cat# 25747-1-AP), PD-1 (cell signaling, Cat # 84651), PD-L1 (Proteintech, Cat# 17952-1-AP), CD30 (Lsbio, cat# LS-c162069) CD3 (cell signaling, cat# 99940), iNOS (invitrogen, cat #PAI-036), Nitrotyrosine (Novus, NBP2-54606) overnight at 4°C, washed with TBST five times, then secondary antibodies were added (Vector Laboratories, cat# PI-2000).

    Blocking Assay:

    Article Title: Activity of the GHRH Antagonist MIA602 and its Underlying Mechanisms of Action in sarcoidosis
    Article Snippet: Confocal immunofluorescence images were acquired using a Leica DM6000 microscope with a SP5 confocal module at the University of Miami McKnight Analytical Imaging Core Facility.Captured images were processed using Velocity Software version 6.1.1 software (Perkin-Elmer, Waltham, MA). .. For immunohistochemistry,5-μm paraffin sections were processed by deparaffinization and rehydration followed by endogenous peroxidase blocking (1% H2 O2 in methanol for 20 minutes) and antigen retrieval (boiled in 10 mM citrate buffer for 30 minutes). .. Tissue sections were blocked with 2% goat or horse serum (Vector Laboratories) and incubated with antibody CD68 (Proteintech, Cat# 25747-1-AP), PD-1 (cell signaling, Cat # 84651), PD-L1 (Proteintech, Cat# 17952-1-AP), CD30 (Lsbio, cat# LS-c162069) CD3 (cell signaling, cat# 99940), iNOS (invitrogen, cat #PAI-036), Nitrotyrosine (Novus, NBP2-54606) overnight at 4°C, washed with TBST five times, then secondary antibodies were added (Vector Laboratories, cat# PI-2000).

    other:

    Article Title: Tumors Resistant to Checkpoint Inhibitors Can Become Sensitive after Treatment with Vascular Disrupting Agents
    Article Snippet: Standard settings were used for deparaffinization and rehydration.

    Article Title: Differential pathogenesis of Usutu virus isolates in mice
    Article Snippet: Following deparaffinization, heat induced antigen retrieval was accomplished by immersing slides in 10 mM sodium citrate buffer (pH 6.0) for 15 minutes.

    Lysis:

    Article Title: Proteomic approach to discover human cancer viruses from formalin-fixed tissues
    Article Snippet: Preparation of FFPE tissue for MS. Deparaffinization was achieved with 2 xylene washes (3 minutes each), rehydrated with serial ethanol washes (100%, 100%, 95%, and 70% for 1 minute each), and washed with LC-MS grade water twice for 3 minutes each. .. After deparaffinization, 100 μL lysis buffer (300 mM Tris [pH 8.0], 100 mM DTT, 2% SDS) was added to each tissue sample, followed by 30 minutes of sonication, 1 hour of incubation at 95°C, and 2 hours of incubation at 65°C. .. After centrifugation at 17,000g for 10 minutes at room temperature, the supernatants containing the extracted proteins were transferred to new eppendorf tubes, and the Pierce 660 nm Protein Assay kit with the IDCR packet (Thermo Fisher Scientific) was used to determine the total protein content.

    Sonication:

    Article Title: Proteomic approach to discover human cancer viruses from formalin-fixed tissues
    Article Snippet: Preparation of FFPE tissue for MS. Deparaffinization was achieved with 2 xylene washes (3 minutes each), rehydrated with serial ethanol washes (100%, 100%, 95%, and 70% for 1 minute each), and washed with LC-MS grade water twice for 3 minutes each. .. After deparaffinization, 100 μL lysis buffer (300 mM Tris [pH 8.0], 100 mM DTT, 2% SDS) was added to each tissue sample, followed by 30 minutes of sonication, 1 hour of incubation at 95°C, and 2 hours of incubation at 65°C. .. After centrifugation at 17,000g for 10 minutes at room temperature, the supernatants containing the extracted proteins were transferred to new eppendorf tubes, and the Pierce 660 nm Protein Assay kit with the IDCR packet (Thermo Fisher Scientific) was used to determine the total protein content.

    Incubation:

    Article Title: Proteomic approach to discover human cancer viruses from formalin-fixed tissues
    Article Snippet: Preparation of FFPE tissue for MS. Deparaffinization was achieved with 2 xylene washes (3 minutes each), rehydrated with serial ethanol washes (100%, 100%, 95%, and 70% for 1 minute each), and washed with LC-MS grade water twice for 3 minutes each. .. After deparaffinization, 100 μL lysis buffer (300 mM Tris [pH 8.0], 100 mM DTT, 2% SDS) was added to each tissue sample, followed by 30 minutes of sonication, 1 hour of incubation at 95°C, and 2 hours of incubation at 65°C. .. After centrifugation at 17,000g for 10 minutes at room temperature, the supernatants containing the extracted proteins were transferred to new eppendorf tubes, and the Pierce 660 nm Protein Assay kit with the IDCR packet (Thermo Fisher Scientific) was used to determine the total protein content.

    Article Title: Long-Term Aerobic Exercise Protects against Cisplatin-Induced Nephrotoxicity by Modulating the Expression of IL-6 and HO-1
    Article Snippet: The bands were analyzed with the software GeneSnap (Syngene, USA) and Gene Tools (Syngene, USA). .. TUNEL assay After deparaffinization and rehydration, kidney sections were incubated with 20 µg/mL proteinase K (Life Technologies), and washed in PBS. ..

    TUNEL Assay:

    Article Title: Long-Term Aerobic Exercise Protects against Cisplatin-Induced Nephrotoxicity by Modulating the Expression of IL-6 and HO-1
    Article Snippet: The bands were analyzed with the software GeneSnap (Syngene, USA) and Gene Tools (Syngene, USA). .. TUNEL assay After deparaffinization and rehydration, kidney sections were incubated with 20 µg/mL proteinase K (Life Technologies), and washed in PBS. ..

    Staining:

    Article Title: The Origin of Amniotic Fluid Monocytes/Macrophages in Women with Intra-Amniotic Inflammation and/or Infection
    Article Snippet: Five-μm-thick sections of formalin-fixed, paraffin-embedded tissue specimens were cut and mounted on SuperFrost Plus microscope slides (Erie Scientific LLC, Portsmouth, NH, USA). .. After deparaffinization, slides were rehydrated and stained with hematoxylin-eosin. ..

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    Thermo Fisher deparaffinization
    Quantification of new bone formation in vivo Rabbit BMSCs were transduced ex vivo with the indicated vectors at an MOI of 1000 particles per cell, loaded onto blocks of absorbable gelatin sponge and incubated for 12 h in osteoblastic differentiation medium to allow the cells to adsorb to the matrix. The gelatin sponge carriers loaded with transduced BMSCs were then implanted between the transverse processes of vertebrae L6 and L7 of New Zealand white rabbits. Animals were sacrificed after 4 weeks and the fusion masses, including the two transverse processes, were harvested. The specimens were fixed, decalcified and cut into 5 micron sections in the sagittal plane, through the middle of the section and including the sponge. After <t>deparaffinization</t> and hydration, sections were stained with hematoxylin and eosin. The area of new bone formed within the region of interest between the two transverse processes was quantified using image analysis software. Data are the means ± standard deviations of values from two sections from four fusion masses per group.
    Deparaffinization, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deparaffinization/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinization - by Bioz Stars, 2021-03
    93/100 stars
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    Image Search Results


    Quantification of new bone formation in vivo Rabbit BMSCs were transduced ex vivo with the indicated vectors at an MOI of 1000 particles per cell, loaded onto blocks of absorbable gelatin sponge and incubated for 12 h in osteoblastic differentiation medium to allow the cells to adsorb to the matrix. The gelatin sponge carriers loaded with transduced BMSCs were then implanted between the transverse processes of vertebrae L6 and L7 of New Zealand white rabbits. Animals were sacrificed after 4 weeks and the fusion masses, including the two transverse processes, were harvested. The specimens were fixed, decalcified and cut into 5 micron sections in the sagittal plane, through the middle of the section and including the sponge. After deparaffinization and hydration, sections were stained with hematoxylin and eosin. The area of new bone formed within the region of interest between the two transverse processes was quantified using image analysis software. Data are the means ± standard deviations of values from two sections from four fusion masses per group.

    Journal: Journal of spinal disorders & techniques

    Article Title: Ex Vivo Transfer of the Hoxc-8-interacting Domain of Smad1 by a Tropism-Modified Adenoviral Vector Results in Efficient Bone Formation in a Rabbit Model of Spinal Fusion

    doi: 10.1097/BSD.0b013e318193b693

    Figure Lengend Snippet: Quantification of new bone formation in vivo Rabbit BMSCs were transduced ex vivo with the indicated vectors at an MOI of 1000 particles per cell, loaded onto blocks of absorbable gelatin sponge and incubated for 12 h in osteoblastic differentiation medium to allow the cells to adsorb to the matrix. The gelatin sponge carriers loaded with transduced BMSCs were then implanted between the transverse processes of vertebrae L6 and L7 of New Zealand white rabbits. Animals were sacrificed after 4 weeks and the fusion masses, including the two transverse processes, were harvested. The specimens were fixed, decalcified and cut into 5 micron sections in the sagittal plane, through the middle of the section and including the sponge. After deparaffinization and hydration, sections were stained with hematoxylin and eosin. The area of new bone formed within the region of interest between the two transverse processes was quantified using image analysis software. Data are the means ± standard deviations of values from two sections from four fusion masses per group.

    Article Snippet: After deparaffinization and hydration, sections were stained with hematoxylin and eosin stains (Richard-Allan Scientific, Kalamazoo, MI).

    Techniques: In Vivo, Ex Vivo, Incubation, Staining, Software

    Visualization of new bone formation in vivo Rabbit BMSCs were transduced ex vivo with the indicated vectors at an MOI of 1000 particles per cell, loaded onto blocks of absorbable gelatin sponge and incubated for 12 h in osteoblastic differentiation medium to allow the cells to adsorb to the matrix. The gelatin sponge carriers loaded with transduced BMSCs were then implanted between the transverse processes of vertebrae L6 and L7 of New Zealand white rabbits. Animals were sacrificed after 4 weeks and the fusion masses, including the two transverse processes, were harvested. The specimens were fixed, decalcified and cut into 5 micron sections in the sagittal plane, through the middle of the section and including the sponge. After deparaffinization and hydration, sections were stained with hematoxylin and eosin. Representative sections are shown.

    Journal: Journal of spinal disorders & techniques

    Article Title: Ex Vivo Transfer of the Hoxc-8-interacting Domain of Smad1 by a Tropism-Modified Adenoviral Vector Results in Efficient Bone Formation in a Rabbit Model of Spinal Fusion

    doi: 10.1097/BSD.0b013e318193b693

    Figure Lengend Snippet: Visualization of new bone formation in vivo Rabbit BMSCs were transduced ex vivo with the indicated vectors at an MOI of 1000 particles per cell, loaded onto blocks of absorbable gelatin sponge and incubated for 12 h in osteoblastic differentiation medium to allow the cells to adsorb to the matrix. The gelatin sponge carriers loaded with transduced BMSCs were then implanted between the transverse processes of vertebrae L6 and L7 of New Zealand white rabbits. Animals were sacrificed after 4 weeks and the fusion masses, including the two transverse processes, were harvested. The specimens were fixed, decalcified and cut into 5 micron sections in the sagittal plane, through the middle of the section and including the sponge. After deparaffinization and hydration, sections were stained with hematoxylin and eosin. Representative sections are shown.

    Article Snippet: After deparaffinization and hydration, sections were stained with hematoxylin and eosin stains (Richard-Allan Scientific, Kalamazoo, MI).

    Techniques: In Vivo, Ex Vivo, Incubation, Staining