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Nichirei deparaffinization
Deparaffinization, supplied by Nichirei, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deparaffinization/product/Nichirei
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deparaffinization - by Bioz Stars, 2021-03
86/100 stars

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Activity Assay:

Article Title: Activation of Src Kinase in Platelet-Derived Growth Factor-B-Dependent Tubular Regeneration after Acute Ischemic Renal Injury
Article Snippet: Immunohistochemical staining was performed by the streptavidin-peroxidase method. .. After deparaffinization and rehydration, sections were treated with distilled water containing 0.3% H2 O2 and 0.1% N2 Na to quench internal peroxidase activity, incubated overnight with anti-active Src kinase monoclonal antibody (clone 28, 0.06 μg/ml) at 4°C, and then subjected to a three-step immunoperoxidase procedure (Histofine kit; Nichirei, Tokyo, Japan). ..

Incubation:

Article Title: Activation of Src Kinase in Platelet-Derived Growth Factor-B-Dependent Tubular Regeneration after Acute Ischemic Renal Injury
Article Snippet: Immunohistochemical staining was performed by the streptavidin-peroxidase method. .. After deparaffinization and rehydration, sections were treated with distilled water containing 0.3% H2 O2 and 0.1% N2 Na to quench internal peroxidase activity, incubated overnight with anti-active Src kinase monoclonal antibody (clone 28, 0.06 μg/ml) at 4°C, and then subjected to a three-step immunoperoxidase procedure (Histofine kit; Nichirei, Tokyo, Japan). ..

Article Title: Immunity of fungal infections alleviated graft reject in liver transplantation compared with non-fungus recipients
Article Snippet: .. After deparaffinization, rehydration, and heating in 95°C buffer, sections were incubated with each antibody and subsequently with Histofine Simple Stain MAX-PO (MULTI) (Nichirei, Japan). .. Incubation was performed overnight at 4°C and followed by a wash in three changes of phosphate buffered saline (PBS) for 5 min. For all staining, the reaction product was developed with the use of 3-diaminobenzidine tetrahydrochloride and H2O2.

Staining:

Article Title: Immunity of fungal infections alleviated graft reject in liver transplantation compared with non-fungus recipients
Article Snippet: .. After deparaffinization, rehydration, and heating in 95°C buffer, sections were incubated with each antibody and subsequently with Histofine Simple Stain MAX-PO (MULTI) (Nichirei, Japan). .. Incubation was performed overnight at 4°C and followed by a wash in three changes of phosphate buffered saline (PBS) for 5 min. For all staining, the reaction product was developed with the use of 3-diaminobenzidine tetrahydrochloride and H2O2.

Immunohistochemistry:

Article Title: Dual-Labeled Near-Infrared/99mTc Imaging Probes Using PAMAM-Coated Silica Nanoparticles for the Imaging of HER2-Expressing Cancer Cells
Article Snippet: Immunohistochemistry Excised tumors were fixed in 10% formaldehyde solution overnight and were embedded paraffin block. .. After deparaffinization, HER2 immunohistochemistry was performed according to a protocol of Histofine® HER2 kit mono atlas (Nichirei Bioscience, Tokyo, Japan) properly with calibrated positive control and negative control. ..

Positive Control:

Article Title: Dual-Labeled Near-Infrared/99mTc Imaging Probes Using PAMAM-Coated Silica Nanoparticles for the Imaging of HER2-Expressing Cancer Cells
Article Snippet: Immunohistochemistry Excised tumors were fixed in 10% formaldehyde solution overnight and were embedded paraffin block. .. After deparaffinization, HER2 immunohistochemistry was performed according to a protocol of Histofine® HER2 kit mono atlas (Nichirei Bioscience, Tokyo, Japan) properly with calibrated positive control and negative control. ..

Negative Control:

Article Title: Dual-Labeled Near-Infrared/99mTc Imaging Probes Using PAMAM-Coated Silica Nanoparticles for the Imaging of HER2-Expressing Cancer Cells
Article Snippet: Immunohistochemistry Excised tumors were fixed in 10% formaldehyde solution overnight and were embedded paraffin block. .. After deparaffinization, HER2 immunohistochemistry was performed according to a protocol of Histofine® HER2 kit mono atlas (Nichirei Bioscience, Tokyo, Japan) properly with calibrated positive control and negative control. ..

Activation Assay:

Article Title: Estradiol/GPER affects the integrity of mammary duct-like structures in vitro
Article Snippet: Immunohistochemical staining Tissue arrays (HBreD060CS05 and BR486) were purchased from US Biomax. .. After deparaffinization and rehydration, antigen retrieval treatment was carried out in an autoclave at 121 °C for 20 min in antigen activation buffer (pH 9.0) (Nichirei Corp.). .. Tissue arrays were blocked in donkey serum for 30 min at room temperature, followed by incubation with primary antibody for 1 h at room temperature.

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    Nichirei deparaffinization
    Deparaffinization, supplied by Nichirei, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deparaffinization/product/Nichirei
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinization - by Bioz Stars, 2021-03
    86/100 stars
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