deparaffinization  (Abcam)

 
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    Name:
    Recombinant Human C 4 Methylsterol Oxidase protein
    Description:

    Catalog Number:
    ab159473
    Price:
    None
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    Structured Review

    Abcam deparaffinization

    https://www.bioz.com/result/deparaffinization/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinization - by Bioz Stars, 2021-03
    86/100 stars

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    Cycling Probe Technology:

    Article Title: Therapeutic Role of Ursolic Acid on Ameliorating Hepatic Steatosis and Improving Metabolic Disorders in High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Rats
    Article Snippet: The nuclear protein from the fresh liver tissues or cultured cells was extracted using a Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime Institute of Biotechnology). .. Antibodies used for protein detecting were bought from the indicated sources, β -actin, and carnitine palmitoyltransferase (CPT)-1 (Santa Cruz Biotechnology, CA), peroxisome proliferator-activated receptor (PPAR)-α, PPAR-γ and sterol regulatory element-binding protein (SREBP)-1c (Abcam, Cambridge, UK), fatty acid translocase (FAT/CD36) and acyl-CoA: diacylglycerol acyltransferase (DGAT)-1 (Life Span, Seattle), histone H3, AMP-activated protein kinase (AMPK) and phosphorylated-AMPK (Cell Sinaling, Boston). ..

    Incubation:

    Article Title: Lgr5 is a potential marker of colorectal carcinoma stem cells that correlates with patient survival
    Article Snippet: Fluorescent immunocytochemistry The cells were immersed in goat serum at room temperature for 15 min to block non-specific binding sites. .. The slides were then incubated with a primary antibody to Lgr5 (Abcam, Cambridge, MA, USA) overnight at 4°C. .. The cells were then washed with PBS and then incubated with a goat anti-rabbit TRITC conjugated secondary antibody (1:50 dilution; Zymed Laboratories, San Francisco, CA, USA) for 2 h at room temperature.

    Article Title: Overexpressed VEPH1 inhibits epithelial-mesenchymal transition, invasion, and migration of human cutaneous melanoma cells through inactivating the TGF-β signaling pathway
    Article Snippet: .. Next, the sections were incubated with diluted primary antibody to VEPH1 (1: 20, ab121875, Abcam, Cambridge, MA, USA) at 4°C overnight. .. The sections were then incubated with streptomycin avidin-peroxidase solution (Zhongshan Biotechnology Co., Ltd., Beijing, China) at 37°C for 30 min and visualized by diaminobenzidine (DAB; Beijing Bioss Biotechnology Co., Ltd., Beijing, China) at room temperature, and soaked in hematoxylin for 5.

    Article Title: 2-[(4-Hydroxybenzyl) Amino] Phenol (HBAP) Restores the Mutated p53 to the Level Similar to That of Wild-Type p53 Protein and Inhibits Breast Cancer Growth in vivo to by Inducing Tumor Cells Apoptosis
    Article Snippet: The cell membrane was permealized with 0.5% Triton X-100 for 5 min to allow the entry of antibody into cells. .. Then the permealized cells were blocked with 1% BSA (bovine serum albumin) for 1 h at room temperature, followed by incubation with the primary antibody PAB1620 or PAB240 (Abcam, United States) at 4°C overnight. ..

    Article Title: Optimization of Circulating Biomarkers of Obatoclax-Induced Cell Death in Patients with Small Cell Lung Cancer
    Article Snippet: Protein lysates were resolved by electrophoresis in appropriate percentage polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon, Millipore, Watford, UK). .. Standard immunoblot analysis procedures were followed with overnight incubation at 4°C with the following primary antibodies: A1 1:1000 (Abcam, Cambridge, UK), Bcl-2 1:1000 (Dako UK Ltd, Ely, UK), Bcl-w 1:1000 (Alexis Biochemical, Lausen, Switzerland), Bcl-xL 1:1000 (BD Transduction Laboratories, Oxford, UK), Mcl-1 1:1000 (BD Biosciences, Oxford, UK), cleaved caspase-3 1:500 (Cell Signaling Technology), cleaved poly (ADP-ribose) polymerase (PARP; Cell Signaling Technology), and tubulin (Cancer Research UK, London). .. Blots were visualized with the enhanced chemiluminescence system (Amersham, Chalfont St. Giles, UK) and analyzed using a Fuji LAS-1000 Plus imaging system with AIDA software (Fuji, Bedford, UK).

    Article Title: Generation of functional hepatocytes from human spermatogonial stem cells
    Article Snippet: For differential plating, cell mixture suspension was seeded into in 10-cm diameter tissue culture dishes in DMEM/F-12 supplemented with 10% fetal bovine serum (FBS) (Gibco) and 103 units/ml leukemia inhibitory factor (LIF, Sigma) and incubated at 34°C in 5% CO2 for 12 hours. .. When Sertoli cells attached to the dishes, human male germ cells remained in suspension and they were collected by centrifuging at 1000 rpm for 5 min. Human male germ cells were incubated with GPR125 (Abcam) at 4°C overnight. .. After washes with BSA-EDTA-PBS (phosphate-buffered saline) buffer, the cells were suspended in 80 μl upon buffer supplemented with 20 μl goat anti-rabbit IgG magnetic microbeads (Miltenyi Biotec) and they were incubated at 4°C for less than 20 min. After incubation, 400 μl of BSA-EDTA-PBS buffer were added to the cells to get a final volume of 500 microliters.

    Article Title: Lipid-laden partially-activated plasmacytoid and CD4−CD8α+ dendritic cells accumulate in tissues in elderly mice
    Article Snippet: For staining molecules associated with lipid uptake, cells were fixed in 1% paraformaldehyde (Sigma-Aldrich) and permeabilised with 0.1% saponin (Sigma-Aldrich). .. Cells were incubated with fluorescently labelled anti-CD36 (Biolegend), anti-CD68 (Biolegend), LRP-1 (Santa Cruz, California, USA), LPL (Abcam, Cambridge, UK) and VLDLr (Abcam) in the dark for 30 mins at 4°C. .. Before staining, mouse IgG1 antibodies (LPL and VLDLr) were directly conjugated to zenon reagents (Invitrogen) as per manufacturer’s instructions.

    Article Title: A signature motif mediating selective interactions of BCL11A with the NR2E/F subfamily of orphan nuclear receptors
    Article Snippet: Immunoprecipitation was performed with PureProteome magnetic beads (Millipore) as per manufacturer’s protocol. .. In all, 10–25 µg of chromatin was used for each IP which was incubated with 10 µg of specific antibody for COUP-TFII (Active motif, 61213) or BCL11A (Abcam, 19489) overnight at 4°C. ..

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  • 93
    Abcam deparaffinization
    Deparaffinization, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deparaffinization/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinization - by Bioz Stars, 2021-03
    93/100 stars
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    86
    Abcam deparaffinized tissue sections
    Deparaffinized Tissue Sections, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deparaffinized tissue sections/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinized tissue sections - by Bioz Stars, 2021-03
    86/100 stars
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    96
    Abcam deparaffinized sections
    Deparaffinized Sections, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deparaffinized sections/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinized sections - by Bioz Stars, 2021-03
    96/100 stars
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