ribonuclease free deoxyribonuclease dnase i  (Roche)


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    Structured Review

    Roche ribonuclease free deoxyribonuclease dnase i
    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, <t>DNAse</t> I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment
    Ribonuclease Free Deoxyribonuclease Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease free deoxyribonuclease dnase i/product/Roche
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ribonuclease free deoxyribonuclease dnase i - by Bioz Stars, 2022-10
    80/100 stars

    Images

    1) Product Images from "Molecular Basis for Glucocorticoid Induction of the Kr?ppel-Like Factor 9 Gene in Hippocampal Neurons"

    Article Title: Molecular Basis for Glucocorticoid Induction of the Kr?ppel-Like Factor 9 Gene in Hippocampal Neurons

    Journal: Endocrinology

    doi: 10.1210/en.2012-1303

    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, DNAse I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment
    Figure Legend Snippet: Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, DNAse I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment

    Techniques Used: Functional Assay

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    Roche ribonuclease free deoxyribonuclease dnase i
    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, <t>DNAse</t> I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment
    Ribonuclease Free Deoxyribonuclease Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease free deoxyribonuclease dnase i/product/Roche
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ribonuclease free deoxyribonuclease dnase i - by Bioz Stars, 2022-10
    80/100 stars
      Buy from Supplier

    90
    Roche deoxyribonuclease i
    Time course of change in NETs by DNase I treatment  in vivo . After the identification of in vivo NETs (red) in postcapillary venules of the cecum of LPS treated mice, DNase I at a dose of 1000 U was administered intravenously via a catheter (DNase I treatment group, n = 5). In control group (n = 5), the equal amount of phosphate buffered saline (PBS) was administered after NETs identification. Images were recorded by a method of 30 sec-imaging followed by 90 sec-pause for at least 30 min (at least 15 cycles) to minimize the photobleaching for NETs during the observation. Anchored NETs with optimal size in postcapillary venules of the cecum were selected to examine the time course of change in NETs by DNase I treatment.   Figure 7  shows in vivo NETs before (  Figure 7-i, iii ) and after (  Figure 7-ii, iv, v ) DNase I treatment.
    Deoxyribonuclease I, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyribonuclease i/product/Roche
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease i - by Bioz Stars, 2022-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, DNAse I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment

    Journal: Endocrinology

    Article Title: Molecular Basis for Glucocorticoid Induction of the Kr?ppel-Like Factor 9 Gene in Hippocampal Neurons

    doi: 10.1210/en.2012-1303

    Figure Lengend Snippet: Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, DNAse I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment

    Article Snippet: We treated 1 μg of total RNA with 20 U of ribonuclease-free deoxyribonuclease (DNAse) I (Roche, Indianapolis, IN) ( ) before cDNA synthesis with the High Capacity Reverse Transcription kit (Invitrogen) with or without the addition of RT.

    Techniques: Functional Assay

    Time course of change in NETs by DNase I treatment  in vivo . After the identification of in vivo NETs (red) in postcapillary venules of the cecum of LPS treated mice, DNase I at a dose of 1000 U was administered intravenously via a catheter (DNase I treatment group, n = 5). In control group (n = 5), the equal amount of phosphate buffered saline (PBS) was administered after NETs identification. Images were recorded by a method of 30 sec-imaging followed by 90 sec-pause for at least 30 min (at least 15 cycles) to minimize the photobleaching for NETs during the observation. Anchored NETs with optimal size in postcapillary venules of the cecum were selected to examine the time course of change in NETs by DNase I treatment.   Figure 7  shows in vivo NETs before (  Figure 7-i, iii ) and after (  Figure 7-ii, iv, v ) DNase I treatment.

    Journal: PLoS ONE

    Article Title: In Vivo Characterization of Neutrophil Extracellular Traps in Various Organs of a Murine Sepsis Model

    doi: 10.1371/journal.pone.0111888

    Figure Lengend Snippet: Time course of change in NETs by DNase I treatment in vivo . After the identification of in vivo NETs (red) in postcapillary venules of the cecum of LPS treated mice, DNase I at a dose of 1000 U was administered intravenously via a catheter (DNase I treatment group, n = 5). In control group (n = 5), the equal amount of phosphate buffered saline (PBS) was administered after NETs identification. Images were recorded by a method of 30 sec-imaging followed by 90 sec-pause for at least 30 min (at least 15 cycles) to minimize the photobleaching for NETs during the observation. Anchored NETs with optimal size in postcapillary venules of the cecum were selected to examine the time course of change in NETs by DNase I treatment. Figure 7 shows in vivo NETs before ( Figure 7-i, iii ) and after ( Figure 7-ii, iv, v ) DNase I treatment.

    Article Snippet: Deoxyribonuclease I (DNase I) was purchased from Roche Applied Science (Mannheim, Germany).

    Techniques: In Vivo, Mouse Assay, Size-exclusion Chromatography, Imaging