Structured Review

Millipore bovine pancreas dnase i
Parasite requirements for TLR9-dependent Leishmania -specific IFN-α/β production of pDCs. (A) IFN-α/β activity (VSV bioassay) in the culture supernatants of FACS-sorted C57BL/6 or TLR9 −/− BM-pDCs stimulated for 48 h with viable L. infantum promastigotes (MOI = 3) in the absence or presence of 500 U/ml DNase I, L. infantum freeze-thaw lysate (MOI = 3, without or with <t>DNase</t> I treatment), 5 μg/ml of synthetic GU-rich ssRNA (without or with DNase I treatment), or 500 U/ml DNase I alone (A, top; mean ± SEM of three experiments) or with L. infantum promastigotes (MOI = 3), L. infantum gDNA, or L. infantum kDNA (A, bottom; mean ± SEM of two experiments). Significant differences (WT vs. KO, gDNA vs. kDNA) are indicated as follows: *, P
Bovine Pancreas Dnase I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bovine pancreas dnase i/product/Millipore
Average 99 stars, based on 10 article reviews
Price from $9.99 to $1999.99
bovine pancreas dnase i - by Bioz Stars, 2022-09
99/100 stars

Images

1) Product Images from "NK cell activation in visceral leishmaniasis requires TLR9, myeloid DCs, and IL-12, but is independent of plasmacytoid DCs"

Article Title: NK cell activation in visceral leishmaniasis requires TLR9, myeloid DCs, and IL-12, but is independent of plasmacytoid DCs

Journal: The Journal of Experimental Medicine

doi: 10.1084/jem.20061293

Parasite requirements for TLR9-dependent Leishmania -specific IFN-α/β production of pDCs. (A) IFN-α/β activity (VSV bioassay) in the culture supernatants of FACS-sorted C57BL/6 or TLR9 −/− BM-pDCs stimulated for 48 h with viable L. infantum promastigotes (MOI = 3) in the absence or presence of 500 U/ml DNase I, L. infantum freeze-thaw lysate (MOI = 3, without or with DNase I treatment), 5 μg/ml of synthetic GU-rich ssRNA (without or with DNase I treatment), or 500 U/ml DNase I alone (A, top; mean ± SEM of three experiments) or with L. infantum promastigotes (MOI = 3), L. infantum gDNA, or L. infantum kDNA (A, bottom; mean ± SEM of two experiments). Significant differences (WT vs. KO, gDNA vs. kDNA) are indicated as follows: *, P
Figure Legend Snippet: Parasite requirements for TLR9-dependent Leishmania -specific IFN-α/β production of pDCs. (A) IFN-α/β activity (VSV bioassay) in the culture supernatants of FACS-sorted C57BL/6 or TLR9 −/− BM-pDCs stimulated for 48 h with viable L. infantum promastigotes (MOI = 3) in the absence or presence of 500 U/ml DNase I, L. infantum freeze-thaw lysate (MOI = 3, without or with DNase I treatment), 5 μg/ml of synthetic GU-rich ssRNA (without or with DNase I treatment), or 500 U/ml DNase I alone (A, top; mean ± SEM of three experiments) or with L. infantum promastigotes (MOI = 3), L. infantum gDNA, or L. infantum kDNA (A, bottom; mean ± SEM of two experiments). Significant differences (WT vs. KO, gDNA vs. kDNA) are indicated as follows: *, P

Techniques Used: Activity Assay, FACS

2) Product Images from "Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis"

Article Title: Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis

Journal: Nature Communications

doi: 10.1038/s41467-018-07550-x

Plasma redox imbalance promotes lung metastasis via NETs. a Experimental schema for ( b – e ): the effect of NETs induced by albumin-thiol blocking on pulmonary metastasis following tail vein injection of CAL-33 HNSCC cells. Saline or IAA (30 mg/kg) was injected intraperitoneally into NSG mice. DNase I or Cl-amidine was administered at the indicated time points. Two day later, SytoxGreen and CAL-33-mCherry cells ( b , c ) or CAL-33-luciferase ( d , e ) were injected. b , c Extracellular DNA (green), CAL-33-mCherry cells (red), and surrounding collagen-rich parenchyma (cyan) within resected lungs were visualized by two-photon microscopy. ( b ) Representative images are shown. Bar = 50 μm. c Quantification of the mCherry-positive volume from two-photon microscopy images of resected lungs. d , e The growth of pulmonary metastases was monitored by bioluminescence imaging. d Representative images are shown. e Luciferase bioluminescence intensity from the lungs over time (0–28 days). f Experimental schema for ( g – i ): the effect of NETs induced by albumin-thiol blocking on a spontaneous pulmonary metastasis model. 4T1 murine mammary carcinoma cells were orthotopically transplanted to the mammary fat pads of female Balb/c mice. Saline or IAA (30 mg/kg) was injected intraperitoneally at the indicated time points. Between 8 to 17 days after transplantation, saline, DNase I, or Cl-amidine was administered daily. g Primary tumor growth. NS not significant. h , i Lungs were removed 30 days after transplantation. h Representative images are shown. i Number of metastatic lung colonies. ( c, i ) Results represent individual values with the mean ± s.d. e , g Results represent the mean ± s.d. c n = 9 per group: three fields in three independent experiments. e n = 5 per group; one of the mice in the DNase I IAA group died on day 1 for unknown reasons. g , i n = 8 per group; one of the mice in the DNase I group died on day 14 for unknown reasons. * P
Figure Legend Snippet: Plasma redox imbalance promotes lung metastasis via NETs. a Experimental schema for ( b – e ): the effect of NETs induced by albumin-thiol blocking on pulmonary metastasis following tail vein injection of CAL-33 HNSCC cells. Saline or IAA (30 mg/kg) was injected intraperitoneally into NSG mice. DNase I or Cl-amidine was administered at the indicated time points. Two day later, SytoxGreen and CAL-33-mCherry cells ( b , c ) or CAL-33-luciferase ( d , e ) were injected. b , c Extracellular DNA (green), CAL-33-mCherry cells (red), and surrounding collagen-rich parenchyma (cyan) within resected lungs were visualized by two-photon microscopy. ( b ) Representative images are shown. Bar = 50 μm. c Quantification of the mCherry-positive volume from two-photon microscopy images of resected lungs. d , e The growth of pulmonary metastases was monitored by bioluminescence imaging. d Representative images are shown. e Luciferase bioluminescence intensity from the lungs over time (0–28 days). f Experimental schema for ( g – i ): the effect of NETs induced by albumin-thiol blocking on a spontaneous pulmonary metastasis model. 4T1 murine mammary carcinoma cells were orthotopically transplanted to the mammary fat pads of female Balb/c mice. Saline or IAA (30 mg/kg) was injected intraperitoneally at the indicated time points. Between 8 to 17 days after transplantation, saline, DNase I, or Cl-amidine was administered daily. g Primary tumor growth. NS not significant. h , i Lungs were removed 30 days after transplantation. h Representative images are shown. i Number of metastatic lung colonies. ( c, i ) Results represent individual values with the mean ± s.d. e , g Results represent the mean ± s.d. c n = 9 per group: three fields in three independent experiments. e n = 5 per group; one of the mice in the DNase I IAA group died on day 1 for unknown reasons. g , i n = 8 per group; one of the mice in the DNase I group died on day 14 for unknown reasons. * P

Techniques Used: Blocking Assay, Injection, Mouse Assay, Luciferase, Microscopy, Imaging, Transplantation Assay

NET-like structures triggered by plasma redox imbalance are lung-predominant. a – c The indicated organs were resected from WT and Alb -/- C57BL/6 mice and NSG mice 2 days post saline or IAA (30 mg/kg) injection with or without single injection of DNase I on day 2, daily injection of Cl-amidine, or single injection of murine albumin on day 0. a , b The resected organs were subjected to two-photon microscopy. Extracellular DNA (green) and surrounding collagen-rich parenchyma (cyan) were visualized by SytoxGreen injected via tail vein 20 min before organ harvest and by second-harmonic generation, respectively. a Representative images are shown. Bar = 40 μm. b Representative images of the lungs from IAA-injected NSG mice with DNase I, Cl-amidine, or albumin treatment. Bar = 40 μm. c Extracts of the organs were subjected to immunoprecipitation using an anti-dsDNA antibody followed by Western blotting using an antibody against Cit-H3. Arrows indicate the molecular weight corresponding to Cit-H3
Figure Legend Snippet: NET-like structures triggered by plasma redox imbalance are lung-predominant. a – c The indicated organs were resected from WT and Alb -/- C57BL/6 mice and NSG mice 2 days post saline or IAA (30 mg/kg) injection with or without single injection of DNase I on day 2, daily injection of Cl-amidine, or single injection of murine albumin on day 0. a , b The resected organs were subjected to two-photon microscopy. Extracellular DNA (green) and surrounding collagen-rich parenchyma (cyan) were visualized by SytoxGreen injected via tail vein 20 min before organ harvest and by second-harmonic generation, respectively. a Representative images are shown. Bar = 40 μm. b Representative images of the lungs from IAA-injected NSG mice with DNase I, Cl-amidine, or albumin treatment. Bar = 40 μm. c Extracts of the organs were subjected to immunoprecipitation using an anti-dsDNA antibody followed by Western blotting using an antibody against Cit-H3. Arrows indicate the molecular weight corresponding to Cit-H3

Techniques Used: Mouse Assay, Injection, Microscopy, Immunoprecipitation, Western Blot, Molecular Weight

3) Product Images from "Mitochondrially targeted Endonuclease III has a powerful anti-infarct effect in an in vivo rat model of myocardial ischemia/reperfusion"

Article Title: Mitochondrially targeted Endonuclease III has a powerful anti-infarct effect in an in vivo rat model of myocardial ischemia/reperfusion

Journal: Basic research in cardiology

doi: 10.1007/s00395-014-0459-0

Infarct size as a % of risk zone in open-chest rats. Open circles represent individual experiments, and closed circles represent group mean ± SEM. DNase I was infused 5 min before reperfusion (rep) and cangrelor infusion was commenced 10 min before
Figure Legend Snippet: Infarct size as a % of risk zone in open-chest rats. Open circles represent individual experiments, and closed circles represent group mean ± SEM. DNase I was infused 5 min before reperfusion (rep) and cangrelor infusion was commenced 10 min before

Techniques Used:

Infarct size as a % of risk zone in isolated, perfused rat hearts with 30 min of regional ischemia. Open circles represent individual experiments, and closed circles represent group mean ± SEM. DNase I was present in the buffer continuously during
Figure Legend Snippet: Infarct size as a % of risk zone in isolated, perfused rat hearts with 30 min of regional ischemia. Open circles represent individual experiments, and closed circles represent group mean ± SEM. DNase I was present in the buffer continuously during

Techniques Used: Isolation

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    Millipore deoxyribonuclease i from bovine pancreas
    Contact-enhanced activation of DCs by DHMs. BMDCs were incubated with DHMs or nmDHMs for 18 hours, after which DHMs/nmDHMs were removed from the well and digested with DNase I. Cells attached to and surrounding DHMs were analyzed for surface marker expression of CD40, CD80, and CD86 by flow cytometry. ** p
    Deoxyribonuclease I From Bovine Pancreas, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyribonuclease i from bovine pancreas/product/Millipore
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease i from bovine pancreas - by Bioz Stars, 2022-09
    99/100 stars
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    95
    Millipore dnase i
    Hsf1 DNA binding is impeded by nucleosomes, both in vitro and in vivo. (A) Hsf1 and H3 ChIP-seq signal at HSP82 under NHS conditions, normalized to their maximum displayed values. The region used for nucleosome reconstitution and <t>DNase</t> I footprinting is highlighted. (B) DNA corresponding to the HSP82 upstream region depicted in A (spanning –9 to –353 [ATG = +1]) and 32 P-end labeled on the upper strand was either reacted directly with GST-Hsf1 (lanes 4–7) or following its reconstitution into a dinucleosome (lanes 9–12). Reconstitution was achieved using a 1:1 (wt/wt) HeLa histone: DNA ratio and salt dilution, followed by purification over a glycerol gradient (see Supplemental Figure S4). Both naked DNA and chromatin templates were challenged with increasing amounts of recombinant Hsf1 (lanes 3 and 8 are -Hsf1 controls) and then subjected to DNase I digestion. DNA was purified and electrophoresed on an 8% sequencing gel. (C) Scatter plots of Hsf1 ChIP-seq signal as a function of the strength of the HSE for the NHS, 5-min HS, and 120-min HS states. HSE strength was determined by MEME as a p value corresponding to how well the binding site beneath the summit of each ChIP peak matched the consensus HSE motif (Figure 1F). ChIP-seq signals represent the mean of two biological replicates. (D) As in C, but here each p value was divided by the H3 ChIP-seq signal below the summit of the Hsf1 peak under NHS conditions (H3 ChIP-seq data from Qiu et al. [2016] ). Outliers (gray) consist of Hsf1-independent housekeeping genes.
    Dnase I, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2022-09
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    Contact-enhanced activation of DCs by DHMs. BMDCs were incubated with DHMs or nmDHMs for 18 hours, after which DHMs/nmDHMs were removed from the well and digested with DNase I. Cells attached to and surrounding DHMs were analyzed for surface marker expression of CD40, CD80, and CD86 by flow cytometry. ** p

    Journal: Advanced healthcare materials

    Article Title: Extracellular trap-mimicking DNA-histone mesostructures synergistically activate dendritic cells

    doi: 10.1002/adhm.201900926

    Figure Lengend Snippet: Contact-enhanced activation of DCs by DHMs. BMDCs were incubated with DHMs or nmDHMs for 18 hours, after which DHMs/nmDHMs were removed from the well and digested with DNase I. Cells attached to and surrounding DHMs were analyzed for surface marker expression of CD40, CD80, and CD86 by flow cytometry. ** p

    Article Snippet: For each replicate, the 36 DHMs were forcefully pipetted into a separate container and centrifuged, after which the supernatant was removed and replaced with 10 U/mL DNase I (Item No D5025, Millipore Sigma) in a Ca2+ and Mg2+-supplemented 10 mM Tris-HCl buffer.

    Techniques: Activation Assay, Incubation, Marker, Expressing, Flow Cytometry

    Parasite requirements for TLR9-dependent Leishmania -specific IFN-α/β production of pDCs. (A) IFN-α/β activity (VSV bioassay) in the culture supernatants of FACS-sorted C57BL/6 or TLR9 −/− BM-pDCs stimulated for 48 h with viable L. infantum promastigotes (MOI = 3) in the absence or presence of 500 U/ml DNase I, L. infantum freeze-thaw lysate (MOI = 3, without or with DNase I treatment), 5 μg/ml of synthetic GU-rich ssRNA (without or with DNase I treatment), or 500 U/ml DNase I alone (A, top; mean ± SEM of three experiments) or with L. infantum promastigotes (MOI = 3), L. infantum gDNA, or L. infantum kDNA (A, bottom; mean ± SEM of two experiments). Significant differences (WT vs. KO, gDNA vs. kDNA) are indicated as follows: *, P

    Journal: The Journal of Experimental Medicine

    Article Title: NK cell activation in visceral leishmaniasis requires TLR9, myeloid DCs, and IL-12, but is independent of plasmacytoid DCs

    doi: 10.1084/jem.20061293

    Figure Lengend Snippet: Parasite requirements for TLR9-dependent Leishmania -specific IFN-α/β production of pDCs. (A) IFN-α/β activity (VSV bioassay) in the culture supernatants of FACS-sorted C57BL/6 or TLR9 −/− BM-pDCs stimulated for 48 h with viable L. infantum promastigotes (MOI = 3) in the absence or presence of 500 U/ml DNase I, L. infantum freeze-thaw lysate (MOI = 3, without or with DNase I treatment), 5 μg/ml of synthetic GU-rich ssRNA (without or with DNase I treatment), or 500 U/ml DNase I alone (A, top; mean ± SEM of three experiments) or with L. infantum promastigotes (MOI = 3), L. infantum gDNA, or L. infantum kDNA (A, bottom; mean ± SEM of two experiments). Significant differences (WT vs. KO, gDNA vs. kDNA) are indicated as follows: *, P

    Article Snippet: In some experiments, ssRNA, Leishmania DNA, or Leishmania lysates were digested with 500 U/ml of bovine pancreas DNase I (Sigma-Aldrich) before their addition to the pDC cultures according to the manufacturer's protocol. gDNA of Leishmania spp. was prepared by proteinase K digestion of promastigotes, followed by phenol/chloroform extraction and ethanol precipitation or by using the Blood & Cell Culture DNA kit (QIAGEN).

    Techniques: Activity Assay, FACS

    Operational schematics of in vivo and in vitro experiments. (A) Schematic diagram of the time axis of intervention in each group of mice. Mice in CON, LPS, and DNase+LPS groups were housed for 5 weeks of “sedentary” feeding and mice in EXE+LPS group were engaged in 5 weeks of aerobic treadmill training. Twenty-four hours after last training, all mice except the CON group were anesthetized and received intraperitoneal administration of 100 μl of Escherichia coli LPS diluted in saline at a dose of 1 μg/μl. Mice in the CON group were submitted to intraperitoneal injection of saline alone, while DNase+LPS mice received aerosolized DNase I 10 μg diluted in 100 μl normal saline at 0 and 12 h after intraperitoneal LPS injection. Then, 24 h later, all mice were anesthetized with 2.5% chloral hydrate (0.1 ml/10 g), and the following analyses were performed. (B) General operation procedures of in vitro experiment: Step 1: Neutrophils were isolated from mouse bone marrow; Step 2: NET formation through PMA stimulation; Step 3: Extraction and purification of NETs by low- and high-speed centrifugation; Step 4: MH-S cells were stimulated with purified NETs to observe polarization reaction.

    Journal: Frontiers in Immunology

    Article Title: Aerobic Exercise Attenuates Acute Lung Injury Through NET Inhibition

    doi: 10.3389/fimmu.2020.00409

    Figure Lengend Snippet: Operational schematics of in vivo and in vitro experiments. (A) Schematic diagram of the time axis of intervention in each group of mice. Mice in CON, LPS, and DNase+LPS groups were housed for 5 weeks of “sedentary” feeding and mice in EXE+LPS group were engaged in 5 weeks of aerobic treadmill training. Twenty-four hours after last training, all mice except the CON group were anesthetized and received intraperitoneal administration of 100 μl of Escherichia coli LPS diluted in saline at a dose of 1 μg/μl. Mice in the CON group were submitted to intraperitoneal injection of saline alone, while DNase+LPS mice received aerosolized DNase I 10 μg diluted in 100 μl normal saline at 0 and 12 h after intraperitoneal LPS injection. Then, 24 h later, all mice were anesthetized with 2.5% chloral hydrate (0.1 ml/10 g), and the following analyses were performed. (B) General operation procedures of in vitro experiment: Step 1: Neutrophils were isolated from mouse bone marrow; Step 2: NET formation through PMA stimulation; Step 3: Extraction and purification of NETs by low- and high-speed centrifugation; Step 4: MH-S cells were stimulated with purified NETs to observe polarization reaction.

    Article Snippet: To investigate the role of DNase I on reducing lung inflammation response, DNase+LPS mice received aerosolized DNase I (Sigma-Aldrich, D5025) 10 μg diluted in 100 μl of normal saline at 0 and 12 h after intraperitoneal LPS injection.

    Techniques: In Vivo, In Vitro, Mouse Assay, Injection, Isolation, Purification, Centrifugation

    NET formation and alveolar macrophage (AM) polarization with aerobic exercise and DNase I treatment. (A) Representative images (magnification ×400) show NET formation of merged DNA (blue), NE (green), and MPO (red) via fluorescence microscopy of lung sections from mice in the CON, LPS, EXE+LPS, and DNase+LPS groups. White circles indicate NETs. (B) MPO–DNA complex levels were detected in BALF of the CON, LPS, EXE+LPS, and DNase+LPS mice; the statistical chart shows that the MPO–DNA complex level of the EXE+LPS and DNase+LPS mice was significantly lower than LPS mice. ** p

    Journal: Frontiers in Immunology

    Article Title: Aerobic Exercise Attenuates Acute Lung Injury Through NET Inhibition

    doi: 10.3389/fimmu.2020.00409

    Figure Lengend Snippet: NET formation and alveolar macrophage (AM) polarization with aerobic exercise and DNase I treatment. (A) Representative images (magnification ×400) show NET formation of merged DNA (blue), NE (green), and MPO (red) via fluorescence microscopy of lung sections from mice in the CON, LPS, EXE+LPS, and DNase+LPS groups. White circles indicate NETs. (B) MPO–DNA complex levels were detected in BALF of the CON, LPS, EXE+LPS, and DNase+LPS mice; the statistical chart shows that the MPO–DNA complex level of the EXE+LPS and DNase+LPS mice was significantly lower than LPS mice. ** p

    Article Snippet: To investigate the role of DNase I on reducing lung inflammation response, DNase+LPS mice received aerosolized DNase I (Sigma-Aldrich, D5025) 10 μg diluted in 100 μl of normal saline at 0 and 12 h after intraperitoneal LPS injection.

    Techniques: Fluorescence, Microscopy, Mouse Assay

    Hsf1 DNA binding is impeded by nucleosomes, both in vitro and in vivo. (A) Hsf1 and H3 ChIP-seq signal at HSP82 under NHS conditions, normalized to their maximum displayed values. The region used for nucleosome reconstitution and DNase I footprinting is highlighted. (B) DNA corresponding to the HSP82 upstream region depicted in A (spanning –9 to –353 [ATG = +1]) and 32 P-end labeled on the upper strand was either reacted directly with GST-Hsf1 (lanes 4–7) or following its reconstitution into a dinucleosome (lanes 9–12). Reconstitution was achieved using a 1:1 (wt/wt) HeLa histone: DNA ratio and salt dilution, followed by purification over a glycerol gradient (see Supplemental Figure S4). Both naked DNA and chromatin templates were challenged with increasing amounts of recombinant Hsf1 (lanes 3 and 8 are -Hsf1 controls) and then subjected to DNase I digestion. DNA was purified and electrophoresed on an 8% sequencing gel. (C) Scatter plots of Hsf1 ChIP-seq signal as a function of the strength of the HSE for the NHS, 5-min HS, and 120-min HS states. HSE strength was determined by MEME as a p value corresponding to how well the binding site beneath the summit of each ChIP peak matched the consensus HSE motif (Figure 1F). ChIP-seq signals represent the mean of two biological replicates. (D) As in C, but here each p value was divided by the H3 ChIP-seq signal below the summit of the Hsf1 peak under NHS conditions (H3 ChIP-seq data from Qiu et al. [2016] ). Outliers (gray) consist of Hsf1-independent housekeeping genes.

    Journal: Molecular Biology of the Cell

    Article Title: Genetic and epigenetic determinants establish a continuum of Hsf1 occupancy and activity across the yeast genome

    doi: 10.1091/mbc.E18-06-0353

    Figure Lengend Snippet: Hsf1 DNA binding is impeded by nucleosomes, both in vitro and in vivo. (A) Hsf1 and H3 ChIP-seq signal at HSP82 under NHS conditions, normalized to their maximum displayed values. The region used for nucleosome reconstitution and DNase I footprinting is highlighted. (B) DNA corresponding to the HSP82 upstream region depicted in A (spanning –9 to –353 [ATG = +1]) and 32 P-end labeled on the upper strand was either reacted directly with GST-Hsf1 (lanes 4–7) or following its reconstitution into a dinucleosome (lanes 9–12). Reconstitution was achieved using a 1:1 (wt/wt) HeLa histone: DNA ratio and salt dilution, followed by purification over a glycerol gradient (see Supplemental Figure S4). Both naked DNA and chromatin templates were challenged with increasing amounts of recombinant Hsf1 (lanes 3 and 8 are -Hsf1 controls) and then subjected to DNase I digestion. DNA was purified and electrophoresed on an 8% sequencing gel. (C) Scatter plots of Hsf1 ChIP-seq signal as a function of the strength of the HSE for the NHS, 5-min HS, and 120-min HS states. HSE strength was determined by MEME as a p value corresponding to how well the binding site beneath the summit of each ChIP peak matched the consensus HSE motif (Figure 1F). ChIP-seq signals represent the mean of two biological replicates. (D) As in C, but here each p value was divided by the H3 ChIP-seq signal below the summit of the Hsf1 peak under NHS conditions (H3 ChIP-seq data from Qiu et al. [2016] ). Outliers (gray) consist of Hsf1-independent housekeeping genes.

    Article Snippet: A 1/100 dilution of DNase I (5 µl; Sigma D7291; 100 U/ µl stock) was then added to each mixture, and digestion was allowed to proceed for 2 min.

    Techniques: Binding Assay, In Vitro, In Vivo, Chromatin Immunoprecipitation, Footprinting, Labeling, Purification, Recombinant, Sequencing