deoxyribonuclease i alexa fluor 488 conjugate  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    Deoxyribonuclease I Alexa Fluor 488 Conjugate
    Description:
    The bright green fluorescent Alexa Fluor 488 DNase I selectively binds to G actin globular and can be used for the detection of unpolymerized actin in fixed cells
    Catalog Number:
    d12371
    Price:
    None
    Applications:
    Cell Analysis|Cytoskeleton|Cell Structure
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher deoxyribonuclease i alexa fluor 488 conjugate
    The bright green fluorescent Alexa Fluor 488 DNase I selectively binds to G actin globular and can be used for the detection of unpolymerized actin in fixed cells
    https://www.bioz.com/result/deoxyribonuclease i alexa fluor 488 conjugate/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease i alexa fluor 488 conjugate - by Bioz Stars, 2020-02
    90/100 stars

    Related Products / Commonly Used Together

    bsa
    atto 550-phalloidin

    Images

    Related Articles

    Transduction:

    Article Title: Urokinase Plasminogen Activator Inhibits HIV Virion Release from Macrophage-Differentiated Chronically Infected Cells via Activation of RhoA and PKC?
    Article Snippet: Hoechst-33342, DNase I Alexa Fluor 488, phalloidin Alexa Fluor 633 were from Molecular Probes (Eugene, OR). .. Hoechst-33342, DNase I Alexa Fluor 488, phalloidin Alexa Fluor 633 were from Molecular Probes (Eugene, OR).

    Article Title: RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿ †
    Article Snippet: Eight-well Lab-Tek II chamber slides (Nunc) were seeded with Vero E6 cells (4 × 104 /well), and the cells were transduced with recombinant baculoviruses (MOI = 50). .. At the same time, G-actin was specifically stained with Alexa Fluor 488-conjugated DNase I (1:500; Invitrogen).

    Immunocytochemistry:

    Article Title: Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization
    Article Snippet: Paragraph title: Lamellipodial formation and immunocytochemistry ... The cells were washed with PBS, fixed with 4% paraformaldehyde for 10 min, and blocked with PBS containing 5% normal goat serum (Abcam) and 0.3% Triton X-100 for 30 min. G-actin and F-actin were stained with Alexa Fluor 488-conjugated DNase I (Life Technologies) and Alexa Fluor 555-conjugated phalloidin (Life Technologies), respectively.

    Cytometry:

    Article Title: Stiffening of Human Skin Fibroblasts with Age
    Article Snippet: For actin staining, Alexa-488-labeled Dnase I and Alexa-647-labeled phalloidin (Invitrogen, Paisley, United Kingdom) were added at concentrations of 161.0 μ M and 6.6 μ M, respectively. .. After two washing steps with PBS, the fluorescence was measured using a FACSCanto flow cytometer (BD Biosciences, San Jose, CA) and analyzed by the software BD FACSDiva 4.0.

    Construct:

    Article Title: The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium
    Article Snippet: To visualize tensin and ILK we used GFP-tagged genomic rescue constructs ( ; ) and Zasp, a GFP gene trap ( ) ( ). .. The DNAse-I staining was performed using a DNAseI–Alexa-Fluor-488 conjugate (0.3 μM, Molecular Probes).

    End-sequence Profiling:

    Article Title: Mechanical stretch-induced vascular hypertrophy occurs through modulation of leptin synthesis-mediated ROS formation and GATA-4 nuclear translocation
    Article Snippet: For F-actin and G-actin ratio study, RPV frozen sections were fixed in 4% formaldehyde, 0.2% Triton x-100 in the cytoskeleton stabilizing PEM buffer (100 mM PIPES, 5 mM EGTA, 2 mM MgCl2, pH 6.9) for 20 min at room temperature. .. Thereafter, the sections were permeabilized, blocked, and stained with the F-actin stain Phalloidin (100 nM; Acti-stain 555 phalloidin, Cytoskeleton, Denver, CO, USA) and the G-actin stain Deoxyribonuclease I (300 nM; Alexa Fluor 488 conjugate, Invitrogen, NY, USA).

    Incubation:

    Article Title: Stiffening of Human Skin Fibroblasts with Age
    Article Snippet: After incubation of fibroblasts to a confluence level of 90%, cells were trypsinized, centrifuged at 10,000 rpm, and washed twice with PBS. .. For actin staining, Alexa-488-labeled Dnase I and Alexa-647-labeled phalloidin (Invitrogen, Paisley, United Kingdom) were added at concentrations of 161.0 μ M and 6.6 μ M, respectively.

    Article Title: Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival *
    Article Snippet: .. Slides were then blocked using 3% BSA and incubated at room temperature in sequential order with deoxyribonuclease I-Alexa Fluor 488 conjugate (Life Technologies, Inc.) and Atto 550-phalloidin (Sigma) to specifically stain G-actin (globular) and F-actin (filaments), respectively. .. Nuclei were stained with Hoechst 33342.

    Article Title: Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxia
    Article Snippet: .. Arteries were incubated overnight at 4°C in the presence of Alexa Fluor phalloidin-568 (5 U/ml, ThermoFisher) to stain F-actin filaments and Alexa Fluor DNAse-488 (9 μg/ml, ThermoFisher) to label G-actin followed by incubation with the nuclear stain TO-PRO3 (0.5 μM, 20 min, room temperature, ThermoFisher). .. Vessels were then washed in ice-cold PBS, mounted on poly- l -lysine slides (ThermoFisher) with Prolong Gold Antifade Mountant (ThermoFisher), and coverslipped.

    Article Title: Low-dose etoposide-treatment induces endoreplication and cell death accompanied by cytoskeletal alterations in A549 cells: Does the response involve senescence? The possible role of vimentin
    Article Snippet: .. After incubation with 0.1 M glycine in DPBS (5 min, RT) and washing with DPBS (2 × 5 min), the cells were incubated with 2 μM phalloidin (Sigma-Aldrich) in DPBS and, subsequently, with DNase I conjugated to Alexa Fluor 488 (Molecular Probes) in DPBS (1:50, 20 min, in the dark). .. The coverslips were then washed with PBS, stained with DAPI, washed again (3 × 5 min) and mounted on glass slides with Aqua Poly/Mount (Polysciences).

    Article Title: Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization
    Article Snippet: The cells were washed with PBS, fixed with 4% paraformaldehyde for 10 min, and blocked with PBS containing 5% normal goat serum (Abcam) and 0.3% Triton X-100 for 30 min. G-actin and F-actin were stained with Alexa Fluor 488-conjugated DNase I (Life Technologies) and Alexa Fluor 555-conjugated phalloidin (Life Technologies), respectively. .. For immunocytochemistry, cells were incubated with an anti-p-Y397-FAK antibody (44-624G, Life Technologies) for 1 h then incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG (Life Technologies) for 1 h. Coverslips were mounted with 50% glycerol, and confocal images were acquired using a confocal laser microscope (FV-300, Olympus).

    Article Title: Mechanical stretch-induced vascular hypertrophy occurs through modulation of leptin synthesis-mediated ROS formation and GATA-4 nuclear translocation
    Article Snippet: Nonspecific binding was blocked by 1% BSA, 0.1% Triton x-100 in PBS for 10 min followed by incubation with anti-leptin antibody [Ob (Y-20) at 1:100 ratio in 1% BSA and 0.05% Tween in PBS] for 1 h. RPV sections were then washed and incubated for another hour at room temperature with Alexa 594-conjugated goat anti-mouse secondary antibody (1:250 in 1% BSA and 0.05% Tween in PBS; Molecular Probes). .. Thereafter, the sections were permeabilized, blocked, and stained with the F-actin stain Phalloidin (100 nM; Acti-stain 555 phalloidin, Cytoskeleton, Denver, CO, USA) and the G-actin stain Deoxyribonuclease I (300 nM; Alexa Fluor 488 conjugate, Invitrogen, NY, USA).

    Article Title: RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿ †
    Article Snippet: Cells were then washed three times with washing buffer (0.1% Tween 20 in DPBS [DPBST]) and incubated with 1:100 dilutions of Alexa Fluor 405 goat anti-mouse immunoglobulin G (IgG) or Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen). .. At the same time, G-actin was specifically stained with Alexa Fluor 488-conjugated DNase I (1:500; Invitrogen).

    Transfection:

    Article Title: Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival *
    Article Snippet: INS-1E cells were cultured on glass slides coated with Matrigel-fibronectin (100 and 2 μg/ml, respectively; Sigma) and transfected with control or Rbfox1 siRNAs for 48 h. Following the same protocol used to analyze insulin secretion, cells were incubated for 1 h in glucose-free RPMI 1640 GlutaMAX-I medium followed by 30-min incubation in glucose-free Krebs-Ringer solution. .. Slides were then blocked using 3% BSA and incubated at room temperature in sequential order with deoxyribonuclease I-Alexa Fluor 488 conjugate (Life Technologies, Inc.) and Atto 550-phalloidin (Sigma) to specifically stain G-actin (globular) and F-actin (filaments), respectively.

    Expressing:

    Article Title: Mechanical stretch-induced vascular hypertrophy occurs through modulation of leptin synthesis-mediated ROS formation and GATA-4 nuclear translocation
    Article Snippet: Immunohistochemistry To visualize leptin expression, 5 μm-thick frozen RPV sections were fixed using 4% formaldehyde and permeabilized using 0.2% Triton X-100. .. Thereafter, the sections were permeabilized, blocked, and stained with the F-actin stain Phalloidin (100 nM; Acti-stain 555 phalloidin, Cytoskeleton, Denver, CO, USA) and the G-actin stain Deoxyribonuclease I (300 nM; Alexa Fluor 488 conjugate, Invitrogen, NY, USA).

    Modification:

    Article Title: Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization
    Article Snippet: Lamellipodial formation and immunocytochemistry Cells were cultured on Matrigel (growth factor-reduced, BD Biosciences)-coated coverslips and serum-starved in low-serum (0.1% fetal bovine serum [FBS]) Dulbecco’s Modified Eagle’s Medium (DMEM) for 24 h. The cells were treated with either fluvoxamine or vehicle (0.1% DMSO) for 15 min and then stimulated with 10% FBS for 15 min to induce lamellipodia formation. .. The cells were washed with PBS, fixed with 4% paraformaldehyde for 10 min, and blocked with PBS containing 5% normal goat serum (Abcam) and 0.3% Triton X-100 for 30 min. G-actin and F-actin were stained with Alexa Fluor 488-conjugated DNase I (Life Technologies) and Alexa Fluor 555-conjugated phalloidin (Life Technologies), respectively.

    Western Blot:

    Article Title: Novel identification of the free fatty acid receptor FFAR1 that promotes contraction in airway smooth muscle
    Article Snippet: Lysates of human brain cerebral cortex obtained from BD Biosciences were used as positive protein controls on immunoblots. .. Alexa Fluor 488-conjugated DNase I, antibiotic-antimycotic, DMEM/F12 medium, fetal bovine serum (FBS), fluo-4 AM, Opti-MEM Pluronic F-127, ProLong gold antifade reagent, and rhodamine-conjugated phalloidin were obtained from Life Technologies. myo -[3 H]inositol (20 Ci/mmol) was obtained from MP Biomedicals.

    Translocation Assay:

    Article Title: Role and regulation of Abelson tyrosine kinase in Crk-associated substrate/profilin-1 interaction and airway smooth muscle contraction
    Article Snippet: The percentage of cells with translocation was calculated as follows: (number of cells with colocalization)/(number of total cells observed) × 100. .. Fluorophores used were rhodamine-phalloidin (Life Technologies, #R415, L/N 1738179) and Alexa fluor-488 DNase I (Life Technologies, #D12371, L/N 38965A).

    Immunohistochemistry:

    Article Title: Mechanical stretch-induced vascular hypertrophy occurs through modulation of leptin synthesis-mediated ROS formation and GATA-4 nuclear translocation
    Article Snippet: Paragraph title: Immunohistochemistry ... Thereafter, the sections were permeabilized, blocked, and stained with the F-actin stain Phalloidin (100 nM; Acti-stain 555 phalloidin, Cytoskeleton, Denver, CO, USA) and the G-actin stain Deoxyribonuclease I (300 nM; Alexa Fluor 488 conjugate, Invitrogen, NY, USA).

    Protease Inhibitor:

    Article Title: Novel identification of the free fatty acid receptor FFAR1 that promotes contraction in airway smooth muscle
    Article Snippet: Protease inhibitor cocktail III was obtained from EMD Biosciences. .. Alexa Fluor 488-conjugated DNase I, antibiotic-antimycotic, DMEM/F12 medium, fetal bovine serum (FBS), fluo-4 AM, Opti-MEM Pluronic F-127, ProLong gold antifade reagent, and rhodamine-conjugated phalloidin were obtained from Life Technologies. myo -[3 H]inositol (20 Ci/mmol) was obtained from MP Biomedicals.

    Article Title: The Rho kinase inhibitor Fasudil up-regulates astrocytic glutamate transport subsequent to actin remodelling in murine cultured astrocytes
    Article Snippet: Complete protease inhibitor cocktail tablets and Lumilight fluorescence solution were obtained from Roche Diagnostics (Castle Hill, Australia). .. Alexa Fluor 488-conjugated DNaseI and Alexa Fluor secondary antibodies were purchased from Molecular Probes (Melbourne, Australia).

    Cell Culture:

    Article Title: Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival *
    Article Snippet: INS-1E cells were cultured on glass slides coated with Matrigel-fibronectin (100 and 2 μg/ml, respectively; Sigma) and transfected with control or Rbfox1 siRNAs for 48 h. Following the same protocol used to analyze insulin secretion, cells were incubated for 1 h in glucose-free RPMI 1640 GlutaMAX-I medium followed by 30-min incubation in glucose-free Krebs-Ringer solution. .. Slides were then blocked using 3% BSA and incubated at room temperature in sequential order with deoxyribonuclease I-Alexa Fluor 488 conjugate (Life Technologies, Inc.) and Atto 550-phalloidin (Sigma) to specifically stain G-actin (globular) and F-actin (filaments), respectively.

    Article Title: Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization
    Article Snippet: Lamellipodial formation and immunocytochemistry Cells were cultured on Matrigel (growth factor-reduced, BD Biosciences)-coated coverslips and serum-starved in low-serum (0.1% fetal bovine serum [FBS]) Dulbecco’s Modified Eagle’s Medium (DMEM) for 24 h. The cells were treated with either fluvoxamine or vehicle (0.1% DMSO) for 15 min and then stimulated with 10% FBS for 15 min to induce lamellipodia formation. .. The cells were washed with PBS, fixed with 4% paraformaldehyde for 10 min, and blocked with PBS containing 5% normal goat serum (Abcam) and 0.3% Triton X-100 for 30 min. G-actin and F-actin were stained with Alexa Fluor 488-conjugated DNase I (Life Technologies) and Alexa Fluor 555-conjugated phalloidin (Life Technologies), respectively.

    other:

    Article Title: The actin cytoskeleton and cytotoxic T lymphocytes: evidence for multiple roles that could affect granule exocytosis-dependent target cell killing
    Article Snippet: Fura-2 AM, calcein AM, latrunculin A, jasplakinolide, Alexa fluor 532-labelled phalloidin, Alexa fluor 488-labelled DNAse 1 and 4′,6-diamidino-2-phenylindol (DAPI) were from Molecular Probes (Eugene, OR, USA).

    Binding Assay:

    Article Title: Urokinase Plasminogen Activator Inhibits HIV Virion Release from Macrophage-Differentiated Chronically Infected Cells via Activation of RhoA and PKC?
    Article Snippet: This concentration reflects uPA binding affinity for uPAR (0.1–1 nM) and its levels determined in pathological conditions , including serum levels in HIV-1+ individuals . .. Hoechst-33342, DNase I Alexa Fluor 488, phalloidin Alexa Fluor 633 were from Molecular Probes (Eugene, OR).

    Article Title: Mechanical stretch-induced vascular hypertrophy occurs through modulation of leptin synthesis-mediated ROS formation and GATA-4 nuclear translocation
    Article Snippet: Nonspecific binding was blocked by 1% BSA, 0.1% Triton x-100 in PBS for 10 min followed by incubation with anti-leptin antibody [Ob (Y-20) at 1:100 ratio in 1% BSA and 0.05% Tween in PBS] for 1 h. RPV sections were then washed and incubated for another hour at room temperature with Alexa 594-conjugated goat anti-mouse secondary antibody (1:250 in 1% BSA and 0.05% Tween in PBS; Molecular Probes). .. Thereafter, the sections were permeabilized, blocked, and stained with the F-actin stain Phalloidin (100 nM; Acti-stain 555 phalloidin, Cytoskeleton, Denver, CO, USA) and the G-actin stain Deoxyribonuclease I (300 nM; Alexa Fluor 488 conjugate, Invitrogen, NY, USA).

    Immunofluorescence:

    Article Title: Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival *
    Article Snippet: Paragraph title: Actin Filaments Immunofluorescence ... Slides were then blocked using 3% BSA and incubated at room temperature in sequential order with deoxyribonuclease I-Alexa Fluor 488 conjugate (Life Technologies, Inc.) and Atto 550-phalloidin (Sigma) to specifically stain G-actin (globular) and F-actin (filaments), respectively.

    Article Title: LMO7 Mediates Cell-Specific Activation of the Rho-Myocardin-Related Transcription Factor-Serum Response Factor Pathway and Plays an Important Role in Breast Cancer Cell Migration ▿
    Article Snippet: Anti-MAL, anti-MRTF-B, and anti-LMO7 antibodies for immunofluorescence staining were prestige antibodies from Sigma. .. Alexa 488-DNase I, Alexa 488-phalloidin and Texas Red-X–phalloidin were obtained from Invitrogen. l -α-Lysophosphatidic acid (LPA) was obtained from Sigma.

    Fluorescence:

    Article Title: Role and regulation of Abelson tyrosine kinase in Crk-associated substrate/profilin-1 interaction and airway smooth muscle contraction
    Article Snippet: The time of image capturing, intensity gaining, and image contrast in both channels were optimally adjusted and kept constant for all experiments to standardize the fluorescence intensity measurements among experiments. .. Fluorophores used were rhodamine-phalloidin (Life Technologies, #R415, L/N 1738179) and Alexa fluor-488 DNase I (Life Technologies, #D12371, L/N 38965A).

    Article Title: Stiffening of Human Skin Fibroblasts with Age
    Article Snippet: For actin staining, Alexa-488-labeled Dnase I and Alexa-647-labeled phalloidin (Invitrogen, Paisley, United Kingdom) were added at concentrations of 161.0 μ M and 6.6 μ M, respectively. .. After two washing steps with PBS, the fluorescence was measured using a FACSCanto flow cytometer (BD Biosciences, San Jose, CA) and analyzed by the software BD FACSDiva 4.0.

    Article Title: Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival *
    Article Snippet: Slides were then blocked using 3% BSA and incubated at room temperature in sequential order with deoxyribonuclease I-Alexa Fluor 488 conjugate (Life Technologies, Inc.) and Atto 550-phalloidin (Sigma) to specifically stain G-actin (globular) and F-actin (filaments), respectively. .. Cells were visualized under a Zeiss Axiovert 200 inverted fluorescence microscope (Carl Zeiss, Zaventem, Belgium) at ×40.

    Article Title: Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxia
    Article Snippet: Paragraph title: Fluorescence Microscopy for F-actin and G-actin in Intact Pulmonary Arteries ... Arteries were incubated overnight at 4°C in the presence of Alexa Fluor phalloidin-568 (5 U/ml, ThermoFisher) to stain F-actin filaments and Alexa Fluor DNAse-488 (9 μg/ml, ThermoFisher) to label G-actin followed by incubation with the nuclear stain TO-PRO3 (0.5 μM, 20 min, room temperature, ThermoFisher).

    Article Title: The Rho kinase inhibitor Fasudil up-regulates astrocytic glutamate transport subsequent to actin remodelling in murine cultured astrocytes
    Article Snippet: Complete protease inhibitor cocktail tablets and Lumilight fluorescence solution were obtained from Roche Diagnostics (Castle Hill, Australia). .. Alexa Fluor 488-conjugated DNaseI and Alexa Fluor secondary antibodies were purchased from Molecular Probes (Melbourne, Australia).

    Isolation:

    Article Title: Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxia
    Article Snippet: Pulmonary arteries (fourth-fifth order) were isolated, cannulated, and subjected to ET-1-dependent vasoconstriction under Ca2+ -permeabilized conditions as described above. .. Arteries were incubated overnight at 4°C in the presence of Alexa Fluor phalloidin-568 (5 U/ml, ThermoFisher) to stain F-actin filaments and Alexa Fluor DNAse-488 (9 μg/ml, ThermoFisher) to label G-actin followed by incubation with the nuclear stain TO-PRO3 (0.5 μM, 20 min, room temperature, ThermoFisher).

    Flow Cytometry:

    Article Title: Stiffening of Human Skin Fibroblasts with Age
    Article Snippet: For actin staining, Alexa-488-labeled Dnase I and Alexa-647-labeled phalloidin (Invitrogen, Paisley, United Kingdom) were added at concentrations of 161.0 μ M and 6.6 μ M, respectively. .. After two washing steps with PBS, the fluorescence was measured using a FACSCanto flow cytometer (BD Biosciences, San Jose, CA) and analyzed by the software BD FACSDiva 4.0.

    Labeling:

    Article Title: Role and regulation of Abelson tyrosine kinase in Crk-associated substrate/profilin-1 interaction and airway smooth muscle contraction
    Article Snippet: The localization of labeled protein was also line scanned using Leica Image software. .. Fluorophores used were rhodamine-phalloidin (Life Technologies, #R415, L/N 1738179) and Alexa fluor-488 DNase I (Life Technologies, #D12371, L/N 38965A).

    Article Title: Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxia
    Article Snippet: On the day of labeling, arteries were fixed in ice-cold 4% paraformaldehyde, rinsed in ice-cold PBS, and permeabilized (0.2% Triton X-100, 30 min at 4°C). .. Arteries were incubated overnight at 4°C in the presence of Alexa Fluor phalloidin-568 (5 U/ml, ThermoFisher) to stain F-actin filaments and Alexa Fluor DNAse-488 (9 μg/ml, ThermoFisher) to label G-actin followed by incubation with the nuclear stain TO-PRO3 (0.5 μM, 20 min, room temperature, ThermoFisher).

    Article Title: Low-dose etoposide-treatment induces endoreplication and cell death accompanied by cytoskeletal alterations in A549 cells: Does the response involve senescence? The possible role of vimentin
    Article Snippet: Paragraph title: G-actin labeling ... After incubation with 0.1 M glycine in DPBS (5 min, RT) and washing with DPBS (2 × 5 min), the cells were incubated with 2 μM phalloidin (Sigma-Aldrich) in DPBS and, subsequently, with DNase I conjugated to Alexa Fluor 488 (Molecular Probes) in DPBS (1:50, 20 min, in the dark).

    Avidin-Biotin Assay:

    Article Title: The Rho kinase inhibitor Fasudil up-regulates astrocytic glutamate transport subsequent to actin remodelling in murine cultured astrocytes
    Article Snippet: EZ-linkTM Sulfo-NHS-Biotin and ImmunoPure Immobilized Avidin were from Pierce (Progen Biosciences, Queensland, Australia). .. Alexa Fluor 488-conjugated DNaseI and Alexa Fluor secondary antibodies were purchased from Molecular Probes (Melbourne, Australia).

    Blocking Assay:

    Article Title: RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿ †
    Article Snippet: After three 5-min washes in DPBS, the cells on the slides were blocked with blocking buffer (10% FBS in DPBS) for 1 h and then incubated with the appropriate primary antibodies for 1 h. Antibodies used included mouse anti-His-tagged antiserum (Serotec), rabbit anti-PML antiserum (Santa Cruz), rabbit anti-SUMO1 antiserum (Santa Cruz), and rabbit anti-Pol II antiserum C21, which recognizes mainly phosphorylated Pol II and was a kind gift from C.-K. Shen (Academia Sinica). .. At the same time, G-actin was specifically stained with Alexa Fluor 488-conjugated DNase I (1:500; Invitrogen).

    FACS:

    Article Title: Stiffening of Human Skin Fibroblasts with Age
    Article Snippet: Paragraph title: FACS analysis ... For actin staining, Alexa-488-labeled Dnase I and Alexa-647-labeled phalloidin (Invitrogen, Paisley, United Kingdom) were added at concentrations of 161.0 μ M and 6.6 μ M, respectively.

    Plasmid Preparation:

    Article Title: The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium
    Article Snippet: The DNAse-I staining was performed using a DNAseI–Alexa-Fluor-488 conjugate (0.3 μM, Molecular Probes). .. The DNAse-I staining was performed using a DNAseI–Alexa-Fluor-488 conjugate (0.3 μM, Molecular Probes).

    Software:

    Article Title: Role and regulation of Abelson tyrosine kinase in Crk-associated substrate/profilin-1 interaction and airway smooth muscle contraction
    Article Snippet: The localization of labeled protein was also line scanned using Leica Image software. .. Fluorophores used were rhodamine-phalloidin (Life Technologies, #R415, L/N 1738179) and Alexa fluor-488 DNase I (Life Technologies, #D12371, L/N 38965A).

    Article Title: Stiffening of Human Skin Fibroblasts with Age
    Article Snippet: For actin staining, Alexa-488-labeled Dnase I and Alexa-647-labeled phalloidin (Invitrogen, Paisley, United Kingdom) were added at concentrations of 161.0 μ M and 6.6 μ M, respectively. .. After two washing steps with PBS, the fluorescence was measured using a FACSCanto flow cytometer (BD Biosciences, San Jose, CA) and analyzed by the software BD FACSDiva 4.0.

    Article Title: Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival *
    Article Snippet: Slides were then blocked using 3% BSA and incubated at room temperature in sequential order with deoxyribonuclease I-Alexa Fluor 488 conjugate (Life Technologies, Inc.) and Atto 550-phalloidin (Sigma) to specifically stain G-actin (globular) and F-actin (filaments), respectively. .. Images were acquired and processed using the AxioVision LE64 software (Carl Zeiss).

    Article Title: Mechanical stretch-induced vascular hypertrophy occurs through modulation of leptin synthesis-mediated ROS formation and GATA-4 nuclear translocation
    Article Snippet: Images were acquired with a laser confocal microscope (LSM710, ZEN confocal software Carl Zeiss). .. Thereafter, the sections were permeabilized, blocked, and stained with the F-actin stain Phalloidin (100 nM; Acti-stain 555 phalloidin, Cytoskeleton, Denver, CO, USA) and the G-actin stain Deoxyribonuclease I (300 nM; Alexa Fluor 488 conjugate, Invitrogen, NY, USA).

    Microscopy:

    Article Title: Role and regulation of Abelson tyrosine kinase in Crk-associated substrate/profilin-1 interaction and airway smooth muscle contraction
    Article Snippet: The cellular localization of fluorescently labeled proteins was viewed under a high resolution digital fluorescent microscopy (Leica, 63× oil objective). .. Fluorophores used were rhodamine-phalloidin (Life Technologies, #R415, L/N 1738179) and Alexa fluor-488 DNase I (Life Technologies, #D12371, L/N 38965A).

    Article Title: Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival *
    Article Snippet: Slides were then blocked using 3% BSA and incubated at room temperature in sequential order with deoxyribonuclease I-Alexa Fluor 488 conjugate (Life Technologies, Inc.) and Atto 550-phalloidin (Sigma) to specifically stain G-actin (globular) and F-actin (filaments), respectively. .. Cells were visualized under a Zeiss Axiovert 200 inverted fluorescence microscope (Carl Zeiss, Zaventem, Belgium) at ×40.

    Article Title: Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxia
    Article Snippet: Paragraph title: Fluorescence Microscopy for F-actin and G-actin in Intact Pulmonary Arteries ... Arteries were incubated overnight at 4°C in the presence of Alexa Fluor phalloidin-568 (5 U/ml, ThermoFisher) to stain F-actin filaments and Alexa Fluor DNAse-488 (9 μg/ml, ThermoFisher) to label G-actin followed by incubation with the nuclear stain TO-PRO3 (0.5 μM, 20 min, room temperature, ThermoFisher).

    Article Title: Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization
    Article Snippet: The cells were washed with PBS, fixed with 4% paraformaldehyde for 10 min, and blocked with PBS containing 5% normal goat serum (Abcam) and 0.3% Triton X-100 for 30 min. G-actin and F-actin were stained with Alexa Fluor 488-conjugated DNase I (Life Technologies) and Alexa Fluor 555-conjugated phalloidin (Life Technologies), respectively. .. For immunocytochemistry, cells were incubated with an anti-p-Y397-FAK antibody (44-624G, Life Technologies) for 1 h then incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG (Life Technologies) for 1 h. Coverslips were mounted with 50% glycerol, and confocal images were acquired using a confocal laser microscope (FV-300, Olympus).

    Article Title: Mechanical stretch-induced vascular hypertrophy occurs through modulation of leptin synthesis-mediated ROS formation and GATA-4 nuclear translocation
    Article Snippet: Images were acquired with a laser confocal microscope (LSM710, ZEN confocal software Carl Zeiss). .. Thereafter, the sections were permeabilized, blocked, and stained with the F-actin stain Phalloidin (100 nM; Acti-stain 555 phalloidin, Cytoskeleton, Denver, CO, USA) and the G-actin stain Deoxyribonuclease I (300 nM; Alexa Fluor 488 conjugate, Invitrogen, NY, USA).

    Article Title: RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿ †
    Article Snippet: At the same time, G-actin was specifically stained with Alexa Fluor 488-conjugated DNase I (1:500; Invitrogen). .. Fluorescent images were visualized with a Zeiss laser confocal microscope (LSM510).

    Recombinant:

    Article Title: RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿ †
    Article Snippet: Eight-well Lab-Tek II chamber slides (Nunc) were seeded with Vero E6 cells (4 × 104 /well), and the cells were transduced with recombinant baculoviruses (MOI = 50). .. At the same time, G-actin was specifically stained with Alexa Fluor 488-conjugated DNase I (1:500; Invitrogen).

    Next-Generation Sequencing:

    Article Title: The Rho kinase inhibitor Fasudil up-regulates astrocytic glutamate transport subsequent to actin remodelling in murine cultured astrocytes
    Article Snippet: Alexa Fluor 488-conjugated DNaseI and Alexa Fluor secondary antibodies were purchased from Molecular Probes (Melbourne, Australia). .. Fasudil, Y27632, NDS, NGS and mouse anti-GFAP antibody were obtained from Chemicon (Melbourne, Australia).

    Laser-Scanning Microscopy:

    Article Title: Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxia
    Article Snippet: Arteries were incubated overnight at 4°C in the presence of Alexa Fluor phalloidin-568 (5 U/ml, ThermoFisher) to stain F-actin filaments and Alexa Fluor DNAse-488 (9 μg/ml, ThermoFisher) to label G-actin followed by incubation with the nuclear stain TO-PRO3 (0.5 μM, 20 min, room temperature, ThermoFisher). .. Arteries were imaged using a Leica laser-scanning microscope (TCS SP5) equipped with a ×63 glycerol immersion objective (numerical aperture: 1.30).

    Concentration Assay:

    Article Title: Urokinase Plasminogen Activator Inhibits HIV Virion Release from Macrophage-Differentiated Chronically Infected Cells via Activation of RhoA and PKC?
    Article Snippet: This concentration reflects uPA binding affinity for uPAR (0.1–1 nM) and its levels determined in pathological conditions , including serum levels in HIV-1+ individuals . .. Hoechst-33342, DNase I Alexa Fluor 488, phalloidin Alexa Fluor 633 were from Molecular Probes (Eugene, OR).

    Article Title: Stiffening of Human Skin Fibroblasts with Age
    Article Snippet: For actin staining, Alexa-488-labeled Dnase I and Alexa-647-labeled phalloidin (Invitrogen, Paisley, United Kingdom) were added at concentrations of 161.0 μ M and 6.6 μ M, respectively. .. Concerning microtubule staining, the cells were incubated for 30 min with Oregon Green 488 conjugated paclitaxel (Invitrogen) at a concentration of 1.0 μ M. Vimentin was detected using an antibody (sc-32322, dilution 1:200) purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Staining:

    Article Title: Urokinase Plasminogen Activator Inhibits HIV Virion Release from Macrophage-Differentiated Chronically Infected Cells via Activation of RhoA and PKC?
    Article Snippet: Hoechst-33342, DNase I Alexa Fluor 488, phalloidin Alexa Fluor 633 were from Molecular Probes (Eugene, OR). .. Cytochalasin D was from Calbiochem (Merck KGaA, Darmstadt, Germany) and was resuspended in DMSO and used at 10 µM, based on preliminary experiments identifying non-toxic concentrations for U1 cells, defined as [3 H]-thymidine uptake and vital cell counts after Trypan blue staining.

    Article Title: Stiffening of Human Skin Fibroblasts with Age
    Article Snippet: .. For actin staining, Alexa-488-labeled Dnase I and Alexa-647-labeled phalloidin (Invitrogen, Paisley, United Kingdom) were added at concentrations of 161.0 μ M and 6.6 μ M, respectively. .. After two washing steps with PBS, the fluorescence was measured using a FACSCanto flow cytometer (BD Biosciences, San Jose, CA) and analyzed by the software BD FACSDiva 4.0.

    Article Title: Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival *
    Article Snippet: .. Slides were then blocked using 3% BSA and incubated at room temperature in sequential order with deoxyribonuclease I-Alexa Fluor 488 conjugate (Life Technologies, Inc.) and Atto 550-phalloidin (Sigma) to specifically stain G-actin (globular) and F-actin (filaments), respectively. .. Nuclei were stained with Hoechst 33342.

    Article Title: Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxia
    Article Snippet: .. Arteries were incubated overnight at 4°C in the presence of Alexa Fluor phalloidin-568 (5 U/ml, ThermoFisher) to stain F-actin filaments and Alexa Fluor DNAse-488 (9 μg/ml, ThermoFisher) to label G-actin followed by incubation with the nuclear stain TO-PRO3 (0.5 μM, 20 min, room temperature, ThermoFisher). .. Vessels were then washed in ice-cold PBS, mounted on poly- l -lysine slides (ThermoFisher) with Prolong Gold Antifade Mountant (ThermoFisher), and coverslipped.

    Article Title: LMO7 Mediates Cell-Specific Activation of the Rho-Myocardin-Related Transcription Factor-Serum Response Factor Pathway and Plays an Important Role in Breast Cancer Cell Migration ▿
    Article Snippet: Anti-MAL, anti-MRTF-B, and anti-LMO7 antibodies for immunofluorescence staining were prestige antibodies from Sigma. .. Alexa 488-DNase I, Alexa 488-phalloidin and Texas Red-X–phalloidin were obtained from Invitrogen. l -α-Lysophosphatidic acid (LPA) was obtained from Sigma.

    Article Title: The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium
    Article Snippet: .. The DNAse-I staining was performed using a DNAseI–Alexa-Fluor-488 conjugate (0.3 μM, Molecular Probes). .. Samples were mounted in Vectashield H-1000 or Vectashield H-1200 with DAPI (Vector Laboratories).

    Article Title: Low-dose etoposide-treatment induces endoreplication and cell death accompanied by cytoskeletal alterations in A549 cells: Does the response involve senescence? The possible role of vimentin
    Article Snippet: In the first case, permeabilization and fixation steps were identical to those described for vimentin staining. .. After incubation with 0.1 M glycine in DPBS (5 min, RT) and washing with DPBS (2 × 5 min), the cells were incubated with 2 μM phalloidin (Sigma-Aldrich) in DPBS and, subsequently, with DNase I conjugated to Alexa Fluor 488 (Molecular Probes) in DPBS (1:50, 20 min, in the dark).

    Article Title: Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization
    Article Snippet: .. The cells were washed with PBS, fixed with 4% paraformaldehyde for 10 min, and blocked with PBS containing 5% normal goat serum (Abcam) and 0.3% Triton X-100 for 30 min. G-actin and F-actin were stained with Alexa Fluor 488-conjugated DNase I (Life Technologies) and Alexa Fluor 555-conjugated phalloidin (Life Technologies), respectively. .. For immunocytochemistry, cells were incubated with an anti-p-Y397-FAK antibody (44-624G, Life Technologies) for 1 h then incubated with Alexa Fluor 488-conjugated goat anti-rabbit IgG (Life Technologies) for 1 h. Coverslips were mounted with 50% glycerol, and confocal images were acquired using a confocal laser microscope (FV-300, Olympus).

    Article Title: Impaired Relaxation of Airway Smooth Muscle in Mice Lacking the Actin-Binding Protein Gelsolin
    Article Snippet: .. After 15 minutes, the cells were fixed, permeabilized, and stained with rhodamine-conjugated phalloidin (Molecular Probes R415; ThermoFisher Scientific, Waltham, MA) and Alexa 488–conjugated DNase I (Molecular Probes ; ThermoFisher Scientific). ..

    Article Title: Mechanical stretch-induced vascular hypertrophy occurs through modulation of leptin synthesis-mediated ROS formation and GATA-4 nuclear translocation
    Article Snippet: .. Thereafter, the sections were permeabilized, blocked, and stained with the F-actin stain Phalloidin (100 nM; Acti-stain 555 phalloidin, Cytoskeleton, Denver, CO, USA) and the G-actin stain Deoxyribonuclease I (300 nM; Alexa Fluor 488 conjugate, Invitrogen, NY, USA). .. All sections were examined and positive intensity signals were quantified using a laser confocal microscope (LSM710, ZEN confocal software Carl Zeiss).

    Article Title: RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells ▿ †
    Article Snippet: .. At the same time, G-actin was specifically stained with Alexa Fluor 488-conjugated DNase I (1:500; Invitrogen). .. After 1 h, cells were washed three times in washing buffer and sealed with aqueous mounting medium (HIS002B; Serotec).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher alexa fluor 488 conjugated deoxyribonuclease i
    Alexa Fluor 488 Conjugated Deoxyribonuclease I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488 conjugated deoxyribonuclease i/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 conjugated deoxyribonuclease i - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results