deoxyribonuclease i alexa fluor 488 conjugate  (Thermo Fisher)


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    Name:
    Deoxyribonuclease I Alexa Fluor 488 Conjugate
    Description:
    The bright green fluorescent Alexa Fluor 488 DNase I selectively binds to G actin globular and can be used for the detection of unpolymerized actin in fixed cells
    Catalog Number:
    d12371
    Price:
    None
    Applications:
    Cell Analysis|Cytoskeleton|Cell Structure
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher deoxyribonuclease i alexa fluor 488 conjugate
    The bright green fluorescent Alexa Fluor 488 DNase I selectively binds to G actin globular and can be used for the detection of unpolymerized actin in fixed cells
    https://www.bioz.com/result/deoxyribonuclease i alexa fluor 488 conjugate/product/Thermo Fisher
    Average 98 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease i alexa fluor 488 conjugate - by Bioz Stars, 2020-09
    98/100 stars

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    Related Articles

    Staining:

    Article Title: YAP/TAZ regulates the expression of proteoglycan 4 and tenascin C in superficial-zone chondrocytes.
    Article Snippet: .. For G- and F-actin staining, samples were incubated for 1 h at room temperature with both Alexa Fluor 488 DNase I conjugate (1 : 500; D12371, Molecular Probes) and Alexa Fluor 598 phalloidin conjugate (1 : 20; A12380, Invitrogen) in 3 % goat serum, 0.1 % Triton X-100 in PBS−/−. .. For YAP/TAZ 52 www.ecmjournal.org detection, samples were incubated overnight at 4 °C with an antibody against YAP/TAZ (1 : 250; 8418S, Cell Signaling) in 3 % goat serum in 0.1 % Triton X-100 in PBS−/−.

    Incubation:

    Article Title: YAP/TAZ regulates the expression of proteoglycan 4 and tenascin C in superficial-zone chondrocytes.
    Article Snippet: .. For G- and F-actin staining, samples were incubated for 1 h at room temperature with both Alexa Fluor 488 DNase I conjugate (1 : 500; D12371, Molecular Probes) and Alexa Fluor 598 phalloidin conjugate (1 : 20; A12380, Invitrogen) in 3 % goat serum, 0.1 % Triton X-100 in PBS−/−. .. For YAP/TAZ 52 www.ecmjournal.org detection, samples were incubated overnight at 4 °C with an antibody against YAP/TAZ (1 : 250; 8418S, Cell Signaling) in 3 % goat serum in 0.1 % Triton X-100 in PBS−/−.

    Amplex Red Cholesterol Assay:

    Article Title: Cholesterol Regulates Glucose-stimulated Insulin Secretion through Phosphatidylinositol 4,5-Bisphosphate *
    Article Snippet: .. Jasplakinolide, Alexa Fluor 488 DNase I, Alexa Fluor 546 phalloidin, the Amplex Red cholesterol assay kit, bis-(1,3-diethylthiobarbituric acid)trimethine oxonol (DiSBAC2 (3)), and Vybrant DiI cell-labeling solution were obtained from Invitrogen. ..

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  • 98
    Thermo Fisher deoxyribonuclease i alexa fluor 488 conjugate
    Deoxyribonuclease I Alexa Fluor 488 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyribonuclease i alexa fluor 488 conjugate/product/Thermo Fisher
    Average 98 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease i alexa fluor 488 conjugate - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

    99
    Thermo Fisher alexa fluor 488 conjugated deoxyribonuclease i
    CAP treatment modulated the cytoskeleton and membrane integrity. Dextran-uptake assay ( A – L ): control-treated cells ( A – D ) and CAP-treated cells ( E – H ), MNNG/HOS ( A , E , I ), U-2 OS ( B , F , J ), A673 ( C , G , K ), and RD-ES ( D , H , L ) cells were seeded on cover slides and allowed to attach for 24 h. After incubation, cells were treated with 15, 30, or 60 s of CAP or carrier gas argon. Fluorescence microscopy was used to analyze of FITC dextran and DAPI. The FITC-positive areas were measured and normalized to the count of DAPI labeled cell cores. G-F-actin assay ( M – X ): MNNG/HOS ( M , Q , U ), U-2 OS ( N , R , V ), A673 ( O , S , W ), and RD-ES ( P , T , X ) cells were seeded on cover slides and allowed to attach for 24 h. After incubation, the cells were treated with 5 s (A673, RD-ES) or 10 s (MNNG/HOS, U-2 OS) of CAP or carrier gas argon. Cells were labeled with TRITC conjugated Phalloidin and <t>Alexa</t> Fluor 488 conjugated Deoxyribonuclease I, and analyzed using fluorescence microscopy. The ratio of the red (TRITC) to green (Deoxyribonuclease I) signal cell was calculated for each. Data show mean ± SD; significant differences were indicated according to a paired t-test as followed: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
    Alexa Fluor 488 Conjugated Deoxyribonuclease I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488 conjugated deoxyribonuclease i/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 conjugated deoxyribonuclease i - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    CAP treatment modulated the cytoskeleton and membrane integrity. Dextran-uptake assay ( A – L ): control-treated cells ( A – D ) and CAP-treated cells ( E – H ), MNNG/HOS ( A , E , I ), U-2 OS ( B , F , J ), A673 ( C , G , K ), and RD-ES ( D , H , L ) cells were seeded on cover slides and allowed to attach for 24 h. After incubation, cells were treated with 15, 30, or 60 s of CAP or carrier gas argon. Fluorescence microscopy was used to analyze of FITC dextran and DAPI. The FITC-positive areas were measured and normalized to the count of DAPI labeled cell cores. G-F-actin assay ( M – X ): MNNG/HOS ( M , Q , U ), U-2 OS ( N , R , V ), A673 ( O , S , W ), and RD-ES ( P , T , X ) cells were seeded on cover slides and allowed to attach for 24 h. After incubation, the cells were treated with 5 s (A673, RD-ES) or 10 s (MNNG/HOS, U-2 OS) of CAP or carrier gas argon. Cells were labeled with TRITC conjugated Phalloidin and Alexa Fluor 488 conjugated Deoxyribonuclease I, and analyzed using fluorescence microscopy. The ratio of the red (TRITC) to green (Deoxyribonuclease I) signal cell was calculated for each. Data show mean ± SD; significant differences were indicated according to a paired t-test as followed: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: An Innovative Therapeutic Option for the Treatment of Skeletal Sarcomas: Elimination of Osteo- and Ewing’s Sarcoma Cells Using Physical Gas Plasma

    doi: 10.3390/ijms21124460

    Figure Lengend Snippet: CAP treatment modulated the cytoskeleton and membrane integrity. Dextran-uptake assay ( A – L ): control-treated cells ( A – D ) and CAP-treated cells ( E – H ), MNNG/HOS ( A , E , I ), U-2 OS ( B , F , J ), A673 ( C , G , K ), and RD-ES ( D , H , L ) cells were seeded on cover slides and allowed to attach for 24 h. After incubation, cells were treated with 15, 30, or 60 s of CAP or carrier gas argon. Fluorescence microscopy was used to analyze of FITC dextran and DAPI. The FITC-positive areas were measured and normalized to the count of DAPI labeled cell cores. G-F-actin assay ( M – X ): MNNG/HOS ( M , Q , U ), U-2 OS ( N , R , V ), A673 ( O , S , W ), and RD-ES ( P , T , X ) cells were seeded on cover slides and allowed to attach for 24 h. After incubation, the cells were treated with 5 s (A673, RD-ES) or 10 s (MNNG/HOS, U-2 OS) of CAP or carrier gas argon. Cells were labeled with TRITC conjugated Phalloidin and Alexa Fluor 488 conjugated Deoxyribonuclease I, and analyzed using fluorescence microscopy. The ratio of the red (TRITC) to green (Deoxyribonuclease I) signal cell was calculated for each. Data show mean ± SD; significant differences were indicated according to a paired t-test as followed: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

    Article Snippet: Cells were stained for 20 min with Rhodamine conjugated Phalloidin (0.022 µM) (Thermo Fisher Scientific, Waltham, MA, USA) and Alexa Fluor 488 conjugated Deoxyribonuclease I (0.3 µM) (Thermo Fisher Scientific, Waltham, MA, USA) in the dark.

    Techniques: Incubation, Fluorescence, Microscopy, Labeling