deoxyribonuclease dnase i  (Thermo Fisher)


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    Structured Review

    Thermo Fisher deoxyribonuclease dnase i
    Deoxyribonuclease Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyribonuclease dnase i/product/Thermo Fisher
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease dnase i - by Bioz Stars, 2020-04
    86/100 stars

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    RNA Extraction:

    Article Title: Fluorescent Reporter Zebrafish Line for Estrogenic Compound Screening Generated Using a CRISPR/Cas9-Mediated Knock-in System.
    Article Snippet: An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health. .. An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health.

    Selection:

    Article Title: Fluorescent Reporter Zebrafish Line for Estrogenic Compound Screening Generated Using a CRISPR/Cas9-Mediated Knock-in System.
    Article Snippet: An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health. .. An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health.

    Fluorescence:

    Article Title: Fluorescent Reporter Zebrafish Line for Estrogenic Compound Screening Generated Using a CRISPR/Cas9-Mediated Knock-in System.
    Article Snippet: An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health. .. An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health.

    Spectrophotometry:

    Article Title: Fluorescent Reporter Zebrafish Line for Estrogenic Compound Screening Generated Using a CRISPR/Cas9-Mediated Knock-in System.
    Article Snippet: An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health. .. An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health.

    Real-time Polymerase Chain Reaction:

    Article Title: Fluorescent Reporter Zebrafish Line for Estrogenic Compound Screening Generated Using a CRISPR/Cas9-Mediated Knock-in System.
    Article Snippet: An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health. .. An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health.

    Imaging:

    Article Title: Fluorescent Reporter Zebrafish Line for Estrogenic Compound Screening Generated Using a CRISPR/Cas9-Mediated Knock-in System.
    Article Snippet: An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health. .. An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health.

    Expressing:

    Article Title: Fluorescent Reporter Zebrafish Line for Estrogenic Compound Screening Generated Using a CRISPR/Cas9-Mediated Knock-in System.
    Article Snippet: An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health. .. An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health.

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    Thermo Fisher deoxyribonuclease dnase
    SHR has impaired nucleic acid clearance capacity, and expression of autophagic machinery is diminished. Activities of ( A ) deoxyribonuclease <t>(DNase)</t> I in serum and DNase II in mesenteric resistance arteries and left ventricle from <t>WKY</t> and SHR. Left , representative
    Deoxyribonuclease Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyribonuclease dnase/product/Thermo Fisher
    Average 99 stars, based on 2092 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease dnase - by Bioz Stars, 2020-04
    99/100 stars
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    SHR has impaired nucleic acid clearance capacity, and expression of autophagic machinery is diminished. Activities of ( A ) deoxyribonuclease (DNase) I in serum and DNase II in mesenteric resistance arteries and left ventricle from WKY and SHR. Left , representative

    Journal: Cardiovascular Research

    Article Title: Circulating mitochondrial DNA and Toll-like receptor 9 are associated with vascular dysfunction in spontaneously hypertensive rats

    doi: 10.1093/cvr/cvv137

    Figure Lengend Snippet: SHR has impaired nucleic acid clearance capacity, and expression of autophagic machinery is diminished. Activities of ( A ) deoxyribonuclease (DNase) I in serum and DNase II in mesenteric resistance arteries and left ventricle from WKY and SHR. Left , representative

    Article Snippet: Deoxyribonuclease (DNase) I and II activity in male WKY and SHR were measured using the single radial enzyme diffusion (SRED) assay, as previously described., For DNase I, 2 μL of serum was loaded into a 2 mm thick 2% agarose gel (Thermo Fisher Scientific) on an agarose-coated polyester Gelbond film (GE Healthcare, Uppsala, Sweden).

    Techniques: Expressing

    Colicin D-based molecular surgery and preparation of Lys3-modivariants. ( A ) Treatment of native tRNA Lys 3 (gray line, modifications indicated as gray dots) with Colicin D generated two fragments with 38 nt length (see PAGE analysis). Differences in migration distance were caused by 2′-3′-cyclic phosphates at the 3′ end of the 5′ fragment. ( B ) Due to similar fragment lengths, a DNA oligonucleotide (darker gray) was hybridized to the 3′ fragment. The corresponding band shift of the double-stranded RNA/DNA hybrid enabled separation and isolation of both tRNA fragments. RNAs were then treated with DNaseI and FastAP. ( C ) Prephosphorylated 5′ and 3′ fragments were subsequently annealed together with unmodified RNAs (black lines) on splint DNA (indicated by the darker gray line, fluorescence marker displayed as a star) which contains a 3′ fluorescein residue. Ligation and DNase treatment yielded mv#1 and mv#2 as shown in the respective PAGE analysis. Evaluation of the fluorescence channel revealed successful digestion of DNA splint in modivariant preparations.

    Journal: Nucleic Acids Research

    Article Title: Double methylation of tRNA-U54 to 2′-O-methylthymidine (Tm) synergistically decreases immune response by Toll-like receptor 7

    doi: 10.1093/nar/gky644

    Figure Lengend Snippet: Colicin D-based molecular surgery and preparation of Lys3-modivariants. ( A ) Treatment of native tRNA Lys 3 (gray line, modifications indicated as gray dots) with Colicin D generated two fragments with 38 nt length (see PAGE analysis). Differences in migration distance were caused by 2′-3′-cyclic phosphates at the 3′ end of the 5′ fragment. ( B ) Due to similar fragment lengths, a DNA oligonucleotide (darker gray) was hybridized to the 3′ fragment. The corresponding band shift of the double-stranded RNA/DNA hybrid enabled separation and isolation of both tRNA fragments. RNAs were then treated with DNaseI and FastAP. ( C ) Prephosphorylated 5′ and 3′ fragments were subsequently annealed together with unmodified RNAs (black lines) on splint DNA (indicated by the darker gray line, fluorescence marker displayed as a star) which contains a 3′ fluorescein residue. Ligation and DNase treatment yielded mv#1 and mv#2 as shown in the respective PAGE analysis. Evaluation of the fluorescence channel revealed successful digestion of DNA splint in modivariant preparations.

    Article Snippet: DNase treatment and purification of RNA fragments To obtain the purified, dephosphorylated native fragment, the DNA/RNA hybrid solution was incubated with 0.1 U/μl FastAP (Thermo Scientific) and 1.25 U/μl DNaseI (Thermo Scientific) for 1 h at 37°C.

    Techniques: Generated, Polyacrylamide Gel Electrophoresis, Migration, Electrophoretic Mobility Shift Assay, Isolation, Fluorescence, Marker, Ligation

    ( A ) Cells (5 × 10 6 ) derived from a postoperative lavage, which contains 80% of LDN, were placed on Poly-L-lysine coated 6 well plate and cultured for 2 hours and SYTOX green was added and immediately observed under fluorescein microscopy. ( B ) the same culture was pretreated with DNAse (100 u/ml) for 5 min before the addition SYTOX green. After the magnetic separation, same number of CD66b(+) LDN ( C ) and CD66b(−) cells ( D ) were cultured for 2 hour and NETs formation was visualized by SYTOX green. ( E ) Purified LDN were cultured for indicated periods and NETs were visualized by SYTOX green. In 3 randomly selected fields, areas of the NETs structure were measured using ImageJ software. Data show mean ± SD. ( F , G ) Purified LDN (5 × 10 6 ) cultured on 6 well plate for 2 hours and stained with SYTOX green were incubated with mAbs to myeloperoxidase ( G ), histone ( H ) as well as control mouse IgG ( F ) at the concentration of 5 μg/ml for 1 hour, washed twice and followed by PE-conjugated secondary antibody for 30 min. In each sample, two photos were taken under optical wavelength filters for FITC or PE and superimposed. White bars show 100 μm.

    Journal: Scientific Reports

    Article Title: Low density neutrophils (LDN) in postoperative abdominal cavity assist the peritoneal recurrence through the production of neutrophil extracellular traps (NETs)

    doi: 10.1038/s41598-017-19091-2

    Figure Lengend Snippet: ( A ) Cells (5 × 10 6 ) derived from a postoperative lavage, which contains 80% of LDN, were placed on Poly-L-lysine coated 6 well plate and cultured for 2 hours and SYTOX green was added and immediately observed under fluorescein microscopy. ( B ) the same culture was pretreated with DNAse (100 u/ml) for 5 min before the addition SYTOX green. After the magnetic separation, same number of CD66b(+) LDN ( C ) and CD66b(−) cells ( D ) were cultured for 2 hour and NETs formation was visualized by SYTOX green. ( E ) Purified LDN were cultured for indicated periods and NETs were visualized by SYTOX green. In 3 randomly selected fields, areas of the NETs structure were measured using ImageJ software. Data show mean ± SD. ( F , G ) Purified LDN (5 × 10 6 ) cultured on 6 well plate for 2 hours and stained with SYTOX green were incubated with mAbs to myeloperoxidase ( G ), histone ( H ) as well as control mouse IgG ( F ) at the concentration of 5 μg/ml for 1 hour, washed twice and followed by PE-conjugated secondary antibody for 30 min. In each sample, two photos were taken under optical wavelength filters for FITC or PE and superimposed. White bars show 100 μm.

    Article Snippet: SYTOX green nucleic acid stain and DNAse I were purchased from Thermo Fisher Scientific (Waltham, MA) and PKH26 was from Sigma-Aldrich (St Louis, MO).

    Techniques: Derivative Assay, Cell Culture, Microscopy, Purification, Software, Staining, Incubation, Concentration Assay

    LDN (5 × 10 6 ) were cultured on poly-L-lysine coated 6 well plate for 2 hours. Three different human gastric cancer cell lines, MKN45, OCUM-1 or NUGC-4, were stained red by PKH26 and 1 × 10 6 cells suspended in 1 ml DMEM were added and incubated for 5 min. After gentle washing, the attached tumor cells were counted under the fluorescein microscope. ( B ) LDN monolayer was pretreated with 100 u/ml DNAse for 5 min just before the addition of tumor cells. ( C ) The number of the attached cells were counted at 3 randomly selected fields and mean ± SD was expressed. P value was less than 0.05 ( D ) Immediately after the adhesion experiment, SYTOX green was added and NETs structure was visualized under the optical wavelength filter for FITC, and superimposed on the photo taken under the wavelength for PKH26. The same experiments were performed for OCUM-1 ( E ) and NUGC-4 ( F ). White bars show 100 μm.

    Journal: Scientific Reports

    Article Title: Low density neutrophils (LDN) in postoperative abdominal cavity assist the peritoneal recurrence through the production of neutrophil extracellular traps (NETs)

    doi: 10.1038/s41598-017-19091-2

    Figure Lengend Snippet: LDN (5 × 10 6 ) were cultured on poly-L-lysine coated 6 well plate for 2 hours. Three different human gastric cancer cell lines, MKN45, OCUM-1 or NUGC-4, were stained red by PKH26 and 1 × 10 6 cells suspended in 1 ml DMEM were added and incubated for 5 min. After gentle washing, the attached tumor cells were counted under the fluorescein microscope. ( B ) LDN monolayer was pretreated with 100 u/ml DNAse for 5 min just before the addition of tumor cells. ( C ) The number of the attached cells were counted at 3 randomly selected fields and mean ± SD was expressed. P value was less than 0.05 ( D ) Immediately after the adhesion experiment, SYTOX green was added and NETs structure was visualized under the optical wavelength filter for FITC, and superimposed on the photo taken under the wavelength for PKH26. The same experiments were performed for OCUM-1 ( E ) and NUGC-4 ( F ). White bars show 100 μm.

    Article Snippet: SYTOX green nucleic acid stain and DNAse I were purchased from Thermo Fisher Scientific (Waltham, MA) and PKH26 was from Sigma-Aldrich (St Louis, MO).

    Techniques: Cell Culture, Staining, Incubation, Microscopy

    ( A ) Cells (5 × 10 6 ) derived from a postoperative lavage, which contains 80% of LDN, were placed on Poly-L-lysine coated 6 well plate and cultured for 2 hours and SYTOX green was added and immediately observed under fluorescein microscopy. ( B ) the same culture was pretreated with DNAse (100 u/ml) for 5 min before the addition SYTOX green. After the magnetic separation, same number of CD66b(+) LDN ( C ) and CD66b(−) cells ( D ) were cultured for 2 hour and NETs formation was visualized by SYTOX green. ( E ) Purified LDN were cultured for indicated periods and NETs were visualized by SYTOX green. In 3 randomly selected fields, areas of the NETs structure were measured using ImageJ software. Data show mean ± SD. ( F , G ) Purified LDN (5 × 10 6 ) cultured on 6 well plate for 2 hours and stained with SYTOX green were incubated with mAbs to myeloperoxidase ( G ), histone ( H ) as well as control mouse IgG ( F ) at the concentration of 5 μg/ml for 1 hour, washed twice and followed by PE-conjugated secondary antibody for 30 min. In each sample, two photos were taken under optical wavelength filters for FITC or PE and superimposed. White bars show 100 μm.

    Journal: Scientific Reports

    Article Title: Low density neutrophils (LDN) in postoperative abdominal cavity assist the peritoneal recurrence through the production of neutrophil extracellular traps (NETs)

    doi: 10.1038/s41598-017-19091-2

    Figure Lengend Snippet: ( A ) Cells (5 × 10 6 ) derived from a postoperative lavage, which contains 80% of LDN, were placed on Poly-L-lysine coated 6 well plate and cultured for 2 hours and SYTOX green was added and immediately observed under fluorescein microscopy. ( B ) the same culture was pretreated with DNAse (100 u/ml) for 5 min before the addition SYTOX green. After the magnetic separation, same number of CD66b(+) LDN ( C ) and CD66b(−) cells ( D ) were cultured for 2 hour and NETs formation was visualized by SYTOX green. ( E ) Purified LDN were cultured for indicated periods and NETs were visualized by SYTOX green. In 3 randomly selected fields, areas of the NETs structure were measured using ImageJ software. Data show mean ± SD. ( F , G ) Purified LDN (5 × 10 6 ) cultured on 6 well plate for 2 hours and stained with SYTOX green were incubated with mAbs to myeloperoxidase ( G ), histone ( H ) as well as control mouse IgG ( F ) at the concentration of 5 μg/ml for 1 hour, washed twice and followed by PE-conjugated secondary antibody for 30 min. In each sample, two photos were taken under optical wavelength filters for FITC or PE and superimposed. White bars show 100 μm.

    Article Snippet: SYTOX green nucleic acid stain and DNAse I were purchased from Thermo Fisher Scientific (Waltham, MA) and PKH26 was from Sigma-Aldrich (St Louis, MO).

    Techniques: Derivative Assay, Cell Culture, Microscopy, Purification, Software, Staining, Incubation, Concentration Assay

    LDN (5 × 10 6 ) were cultured on poly-L-lysine coated 6 well plate for 2 hours. Three different human gastric cancer cell lines, MKN45, OCUM-1 or NUGC-4, were stained red by PKH26 and 1 × 10 6 cells suspended in 1 ml DMEM were added and incubated for 5 min. After gentle washing, the attached tumor cells were counted under the fluorescein microscope. ( B ) LDN monolayer was pretreated with 100 u/ml DNAse for 5 min just before the addition of tumor cells. ( C ) The number of the attached cells were counted at 3 randomly selected fields and mean ± SD was expressed. P value was less than 0.05 ( D ) Immediately after the adhesion experiment, SYTOX green was added and NETs structure was visualized under the optical wavelength filter for FITC, and superimposed on the photo taken under the wavelength for PKH26. The same experiments were performed for OCUM-1 ( E ) and NUGC-4 ( F ). White bars show 100 μm.

    Journal: Scientific Reports

    Article Title: Low density neutrophils (LDN) in postoperative abdominal cavity assist the peritoneal recurrence through the production of neutrophil extracellular traps (NETs)

    doi: 10.1038/s41598-017-19091-2

    Figure Lengend Snippet: LDN (5 × 10 6 ) were cultured on poly-L-lysine coated 6 well plate for 2 hours. Three different human gastric cancer cell lines, MKN45, OCUM-1 or NUGC-4, were stained red by PKH26 and 1 × 10 6 cells suspended in 1 ml DMEM were added and incubated for 5 min. After gentle washing, the attached tumor cells were counted under the fluorescein microscope. ( B ) LDN monolayer was pretreated with 100 u/ml DNAse for 5 min just before the addition of tumor cells. ( C ) The number of the attached cells were counted at 3 randomly selected fields and mean ± SD was expressed. P value was less than 0.05 ( D ) Immediately after the adhesion experiment, SYTOX green was added and NETs structure was visualized under the optical wavelength filter for FITC, and superimposed on the photo taken under the wavelength for PKH26. The same experiments were performed for OCUM-1 ( E ) and NUGC-4 ( F ). White bars show 100 μm.

    Article Snippet: SYTOX green nucleic acid stain and DNAse I were purchased from Thermo Fisher Scientific (Waltham, MA) and PKH26 was from Sigma-Aldrich (St Louis, MO).

    Techniques: Cell Culture, Staining, Incubation, Microscopy