deoxynucleotide triphosphate  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher deoxynucleotide triphosphate
    Deoxynucleotide Triphosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/Thermo Fisher
    Average 99 stars, based on 340 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-10
    99/100 stars

    Images

    Related Articles

    SYBR Green Assay:

    Article Title: Upregulation of lncRNA HOTAIR contributes to IL-1β-induced MMP overexpression and chondrocytes apoptosis in temporomandibular joint osteoarthritis.
    Article Snippet: .. Individual RT-PCR reactions were carried out in a final 25 μL reaction mixture contained 1 μL of cDNA from samples, 12.5 μL of 2× SYBR Green qPCR Master Mix, 1 μL primers (10mM), and 10.5 μL of RNase/DNase free water. .. Amplification conditions included a pre-incubation at 95 °C for 1 min followed by 40 cycles of 10 s at 95 °C and 20 s at 60 °C.

    Amplification:

    Article Title: Haplotype of RNASE 3 polymorphisms is associated with severe malaria in an Indian population
    Article Snippet: .. In brief, RNASE 3 gene fragments were amplified separately in 50μl reactions including 200ng of each forward and reverse primers of set-1 and set-2, 5μl of 10x PCR buffer, 2μl of dNTPs (10mM), 2μl of template DNA and 3 units of Taq® DNA polymerase, reaction volume was raised by PCR-grade water. .. The reaction volume was prepared with PCR-grade water.

    Blocking Assay:

    Article Title: Programmable low-cost DNA-based platform for viral RNA detection
    Article Snippet: .. The detection reaction volume is 10 μl with nanoswitch (0.1 nM), MgCl2 (10mM), 1×PBS and blocking oligos (200 nM). .. The blocking oligos are short oligos (14 nucleotides) that can prevent the binding of target RNA to the inner surface of plastic tubes.

    Real-time Polymerase Chain Reaction:

    Article Title: Upregulation of lncRNA HOTAIR contributes to IL-1β-induced MMP overexpression and chondrocytes apoptosis in temporomandibular joint osteoarthritis.
    Article Snippet: .. Individual RT-PCR reactions were carried out in a final 25 μL reaction mixture contained 1 μL of cDNA from samples, 12.5 μL of 2× SYBR Green qPCR Master Mix, 1 μL primers (10mM), and 10.5 μL of RNase/DNase free water. .. Amplification conditions included a pre-incubation at 95 °C for 1 min followed by 40 cycles of 10 s at 95 °C and 20 s at 60 °C.

    Polymerase Chain Reaction:

    Article Title: Haplotype of RNASE 3 polymorphisms is associated with severe malaria in an Indian population
    Article Snippet: .. In brief, RNASE 3 gene fragments were amplified separately in 50μl reactions including 200ng of each forward and reverse primers of set-1 and set-2, 5μl of 10x PCR buffer, 2μl of dNTPs (10mM), 2μl of template DNA and 3 units of Taq® DNA polymerase, reaction volume was raised by PCR-grade water. .. The reaction volume was prepared with PCR-grade water.

    Article Title: Preliminary screening of the aqueous extracts of twenty-three different seaweed species in Sri Lanka with in-vitro and in-vivo assays
    Article Snippet: .. Polymerase chain reactions (PCR) were performed in 25 μl of the total volume containing 5×PCR buffer, 25 mM MgCl2, 2 mM deoxynucleotide triphosphate (dNTP), 10 μM forward and reverse primers, and Taq DNA polymerase (0.25μl). ..

    Article Title: Mitochondrial haplogroups and expression studies of commonly used human cell lines.
    Article Snippet: .. We developed a multiplex fragment length analysis (MFLA) for clearly assigning mitochondrial haplogroups mostly endemic in Europe for future cardiac diagnostics. .. We developed a multiplex fragment length analysis (MFLA) for clearly assigning mitochondrial haplogroups mostly endemic in Europe for future cardiac diagnostics.

    other:

    Article Title: Overcoming challenges in human saliva gene expression measurements
    Article Snippet: Total RNA was reverse transcribed in 100 µl of total reaction mixture containing 10 µl of 10 × RT buffer, 4 µl of 10 mM deoxynucleotide triphosphate (dNTP), 10 µl of random primers, 21 µl of RNase free water and, 5 µl of reverse transcriptase enzyme.

    Article Title: Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation
    Article Snippet: Reaction was carried out in 25 μL of final volume containing 1X buffer, deoxynucleotide triphosphate (dNTP) 0.2 mM, 0.4 μM primers, MgCl2 2.5 mM, Taq Polymerase 1U and 5 μL of DNA eluate.

    Article Title: Integration of Transcriptome and Small RNA Sequencing to Decipher Molecular Interaction of Chenopodium quinoa Varieties with Cucumber Mosaic Virus
    Article Snippet: Briefly, 1-2 µg total RNA was added to 0.5 µl dNTP (10mM), 1 µl stem-loop primer (10µM) (Supplementary Table S1) and desired volume of RNase/DNase-free water to a total of 13.8 µl.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Upregulation of lncRNA HOTAIR contributes to IL-1β-induced MMP overexpression and chondrocytes apoptosis in temporomandibular joint osteoarthritis.
    Article Snippet: .. Individual RT-PCR reactions were carried out in a final 25 μL reaction mixture contained 1 μL of cDNA from samples, 12.5 μL of 2× SYBR Green qPCR Master Mix, 1 μL primers (10mM), and 10.5 μL of RNase/DNase free water. .. Amplification conditions included a pre-incubation at 95 °C for 1 min followed by 40 cycles of 10 s at 95 °C and 20 s at 60 °C.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher gene exp samhd1 hs00210019 m1
    Proposed model for p21-mediated HIV-1 restriction in MDDCs. The expression of p21 is induced during MDDC maturation (step 1). The increase in p21 expression leads to the downregulation of several enzymes (RNR2, TYMS, and TK1) involved in dNTP biosynthesis (step 2), which would decrease the dNTP pool size (step 3), impairing HIV-1 reverse transcription (step 4). In parallel, p21 induction could increase the active isoform of <t>SAMHD1</t> (step 5), which would further block viral replication through the degradation of dNTPs (step 6) or exonuclease activity (step 7). Low levels of dNTP synthesis as a consequence of p21 induction might further facilitate the exonuclease activity of SAMHD1 (step 8). Although not explored here, induction of p21 might also directly block reverse transcriptase activity and impair HIV transcription, as has been shown in CD4 + ).
    Gene Exp Samhd1 Hs00210019 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp samhd1 hs00210019 m1/product/Thermo Fisher
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    gene exp samhd1 hs00210019 m1 - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    92
    Thermo Fisher deoxynucleotide triphosphate
    Proposed model for p21-mediated HIV-1 restriction in MDDCs. The expression of p21 is induced during MDDC maturation (step 1). The increase in p21 expression leads to the downregulation of several enzymes (RNR2, TYMS, and TK1) involved in dNTP biosynthesis (step 2), which would decrease the dNTP pool size (step 3), impairing HIV-1 reverse transcription (step 4). In parallel, p21 induction could increase the active isoform of <t>SAMHD1</t> (step 5), which would further block viral replication through the degradation of dNTPs (step 6) or exonuclease activity (step 7). Low levels of dNTP synthesis as a consequence of p21 induction might further facilitate the exonuclease activity of SAMHD1 (step 8). Although not explored here, induction of p21 might also directly block reverse transcriptase activity and impair HIV transcription, as has been shown in CD4 + ).
    Deoxynucleotide Triphosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/Thermo Fisher
    Average 92 stars, based on 340 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    Image Search Results


    Proposed model for p21-mediated HIV-1 restriction in MDDCs. The expression of p21 is induced during MDDC maturation (step 1). The increase in p21 expression leads to the downregulation of several enzymes (RNR2, TYMS, and TK1) involved in dNTP biosynthesis (step 2), which would decrease the dNTP pool size (step 3), impairing HIV-1 reverse transcription (step 4). In parallel, p21 induction could increase the active isoform of SAMHD1 (step 5), which would further block viral replication through the degradation of dNTPs (step 6) or exonuclease activity (step 7). Low levels of dNTP synthesis as a consequence of p21 induction might further facilitate the exonuclease activity of SAMHD1 (step 8). Although not explored here, induction of p21 might also directly block reverse transcriptase activity and impair HIV transcription, as has been shown in CD4 + ).

    Journal: Journal of Virology

    Article Title: p21 Restricts HIV-1 in Monocyte-Derived Dendritic Cells through the Reduction of Deoxynucleoside Triphosphate Biosynthesis and Regulation of SAMHD1 Antiviral Activity

    doi: 10.1128/JVI.01324-17

    Figure Lengend Snippet: Proposed model for p21-mediated HIV-1 restriction in MDDCs. The expression of p21 is induced during MDDC maturation (step 1). The increase in p21 expression leads to the downregulation of several enzymes (RNR2, TYMS, and TK1) involved in dNTP biosynthesis (step 2), which would decrease the dNTP pool size (step 3), impairing HIV-1 reverse transcription (step 4). In parallel, p21 induction could increase the active isoform of SAMHD1 (step 5), which would further block viral replication through the degradation of dNTPs (step 6) or exonuclease activity (step 7). Low levels of dNTP synthesis as a consequence of p21 induction might further facilitate the exonuclease activity of SAMHD1 (step 8). Although not explored here, induction of p21 might also directly block reverse transcriptase activity and impair HIV transcription, as has been shown in CD4 + ).

    Article Snippet: PCR amplification of cDNAs was carried out as previously described ( ) on a 7500 real-time PCR system using the TaqMan gene expression premade mix (Applied Biosystems) to amplify the following genes: RRM2 (Hs00357247_g1), TYMS (Hs00426586_m1), SAMHD1 (Hs00210019_m1), CTPS1 (Hs01041858_m1), TK1 (Hs01062125_m1), CDKN1A (Hs00355782_m1), and GAPDH (Hs02758991_g1).

    Techniques: Expressing, Blocking Assay, Activity Assay

    p21 expression modulates SAMHD1 phosphorylation levels in MDDCs. (A to C) Relative copies of SAMHD1 mRNA (A), total SAMHD1 protein levels (B), and pSAMHD1 protein levels (C) in immature and mature MDDC after transfection with siRNA p21 or siRNA NT. The data are expressed relative to the number of mRNA copies or protein levels in immature MDDCs transfected with nontargeting siRNA. The symbols represent independent experiments ( n = 9 donors for mRNA and n = 4 donors for proteins). (D) Changes in luciferase activity after infection with HIV-1 NL4.3ΔenvLuc VSV-G in immature and mature MDDCs cultured in the presence of virus-like particles with or without the SIV VPX protein. The results are shown as the fold change relative to the luciferase activity in immature MDDCs cultured with VLPs not carrying VPX. The symbols represent independent experiments ( n = 9). The median and IQR values are shown. P values of ANOVA are shown. Significant differences ( P

    Journal: Journal of Virology

    Article Title: p21 Restricts HIV-1 in Monocyte-Derived Dendritic Cells through the Reduction of Deoxynucleoside Triphosphate Biosynthesis and Regulation of SAMHD1 Antiviral Activity

    doi: 10.1128/JVI.01324-17

    Figure Lengend Snippet: p21 expression modulates SAMHD1 phosphorylation levels in MDDCs. (A to C) Relative copies of SAMHD1 mRNA (A), total SAMHD1 protein levels (B), and pSAMHD1 protein levels (C) in immature and mature MDDC after transfection with siRNA p21 or siRNA NT. The data are expressed relative to the number of mRNA copies or protein levels in immature MDDCs transfected with nontargeting siRNA. The symbols represent independent experiments ( n = 9 donors for mRNA and n = 4 donors for proteins). (D) Changes in luciferase activity after infection with HIV-1 NL4.3ΔenvLuc VSV-G in immature and mature MDDCs cultured in the presence of virus-like particles with or without the SIV VPX protein. The results are shown as the fold change relative to the luciferase activity in immature MDDCs cultured with VLPs not carrying VPX. The symbols represent independent experiments ( n = 9). The median and IQR values are shown. P values of ANOVA are shown. Significant differences ( P

    Article Snippet: PCR amplification of cDNAs was carried out as previously described ( ) on a 7500 real-time PCR system using the TaqMan gene expression premade mix (Applied Biosystems) to amplify the following genes: RRM2 (Hs00357247_g1), TYMS (Hs00426586_m1), SAMHD1 (Hs00210019_m1), CTPS1 (Hs01041858_m1), TK1 (Hs01062125_m1), CDKN1A (Hs00355782_m1), and GAPDH (Hs02758991_g1).

    Techniques: Expressing, Transfection, Luciferase, Activity Assay, Infection, Cell Culture

    Phosphorylation state of SAMHD1. Monocytes (Monocyte), M-CSF-differentiated macrophages (Macrophage (M-CSF)), and GM-CSF-differentiated macrophages (Macrophage (GM-CSF)) were treated with GH123-Nhe/VSV-G for 2 h and incubated at 37°C for 4 h (4 h) or 24 h (24 h), or mock-treated (−). Cells were lyzed and subjected for SDS-PAGE containing Phos-tag to separate phosphorylated proteins from nonphosphorylated ones. SAMHD1 proteins were detected by anti-SAMHD1 antibody. Upper bands shown by a vertical bar and a lower band shown by an arrow represent phosphorylated and nonphosphorylated SAMHD1, respectively. Ratios of phosphorylated SAMHD1 levels to total SAMHD1 levels (ratio) are shown in vertical numbers.

    Journal: PLoS ONE

    Article Title: Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages

    doi: 10.1371/journal.pone.0090969

    Figure Lengend Snippet: Phosphorylation state of SAMHD1. Monocytes (Monocyte), M-CSF-differentiated macrophages (Macrophage (M-CSF)), and GM-CSF-differentiated macrophages (Macrophage (GM-CSF)) were treated with GH123-Nhe/VSV-G for 2 h and incubated at 37°C for 4 h (4 h) or 24 h (24 h), or mock-treated (−). Cells were lyzed and subjected for SDS-PAGE containing Phos-tag to separate phosphorylated proteins from nonphosphorylated ones. SAMHD1 proteins were detected by anti-SAMHD1 antibody. Upper bands shown by a vertical bar and a lower band shown by an arrow represent phosphorylated and nonphosphorylated SAMHD1, respectively. Ratios of phosphorylated SAMHD1 levels to total SAMHD1 levels (ratio) are shown in vertical numbers.

    Article Snippet: For real time PCR, each 20-µL reaction mixture consisted of 5 µL of cDNA, 10 µL TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 µL of TaqMan Gene Ex Assays (Assay ID: Hs00210019_m1).

    Techniques: Incubation, SDS Page

    Quantification of SAMHD1 mRNA expression. Monocytes were differentiated into macrophages for 6 days in the presence of GM-CSF or M-CSF. Macrophages or monocytes were treated with or without VSV-G-pseudotyped and Env-defective HIV-2 particles (GH123/VSV-G), and harvested. SAMHD1 mRNA expression levels were detected by real-time RT-PCR and normalized against GAPDH. Data are shown as mean ± SD.

    Journal: PLoS ONE

    Article Title: Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages

    doi: 10.1371/journal.pone.0090969

    Figure Lengend Snippet: Quantification of SAMHD1 mRNA expression. Monocytes were differentiated into macrophages for 6 days in the presence of GM-CSF or M-CSF. Macrophages or monocytes were treated with or without VSV-G-pseudotyped and Env-defective HIV-2 particles (GH123/VSV-G), and harvested. SAMHD1 mRNA expression levels were detected by real-time RT-PCR and normalized against GAPDH. Data are shown as mean ± SD.

    Article Snippet: For real time PCR, each 20-µL reaction mixture consisted of 5 µL of cDNA, 10 µL TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 µL of TaqMan Gene Ex Assays (Assay ID: Hs00210019_m1).

    Techniques: Expressing, Quantitative RT-PCR

    Effects of SAMHD1 siRNA on HIV-1 infection. Monocytes (Monocyte) or macrophages (Macrophage) were treated with SAMHD1- (siSAMHD1) or GFP- (siGFP) targeting siRNAs. Three days after transfection, cells were harvested or infected with NL43-Luci/VSV-G virus. SAMHD1 mRNA expression levels in harvested cells were detected by real-time RT-PCR and normalized against GAPDH. Data are shown as mean ± SD. Luciferase activity in infected cells was measured 4 days after infection. Data are plotted as the mean ± SD of triplicate samples; presented data are representative of two independent experiments using two donors. A: Results of samples obtained from a donor 1. B: Results of samples obtained from a donor 2.

    Journal: PLoS ONE

    Article Title: Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages

    doi: 10.1371/journal.pone.0090969

    Figure Lengend Snippet: Effects of SAMHD1 siRNA on HIV-1 infection. Monocytes (Monocyte) or macrophages (Macrophage) were treated with SAMHD1- (siSAMHD1) or GFP- (siGFP) targeting siRNAs. Three days after transfection, cells were harvested or infected with NL43-Luci/VSV-G virus. SAMHD1 mRNA expression levels in harvested cells were detected by real-time RT-PCR and normalized against GAPDH. Data are shown as mean ± SD. Luciferase activity in infected cells was measured 4 days after infection. Data are plotted as the mean ± SD of triplicate samples; presented data are representative of two independent experiments using two donors. A: Results of samples obtained from a donor 1. B: Results of samples obtained from a donor 2.

    Article Snippet: For real time PCR, each 20-µL reaction mixture consisted of 5 µL of cDNA, 10 µL TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 µL of TaqMan Gene Ex Assays (Assay ID: Hs00210019_m1).

    Techniques: Infection, Transfection, Expressing, Quantitative RT-PCR, Luciferase, Activity Assay

    Western blot analysis of SAMHD1 in undifferentiated monocytes and macrophages. Monocytes were differentiated into macrophages for 6 days in the presence of GM-CSF or M-CSF. Macrophages or monocytes were treated with or without VSV-G-pseudotyped and Env-defective HIV-2 particles (GH123/VSV-G), and harvested. Whole-cell extracts were separated on SDS-PAGE and analyzed by western blot using the indicated antibodies. Presented data are representative of two independent experiments.

    Journal: PLoS ONE

    Article Title: Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages

    doi: 10.1371/journal.pone.0090969

    Figure Lengend Snippet: Western blot analysis of SAMHD1 in undifferentiated monocytes and macrophages. Monocytes were differentiated into macrophages for 6 days in the presence of GM-CSF or M-CSF. Macrophages or monocytes were treated with or without VSV-G-pseudotyped and Env-defective HIV-2 particles (GH123/VSV-G), and harvested. Whole-cell extracts were separated on SDS-PAGE and analyzed by western blot using the indicated antibodies. Presented data are representative of two independent experiments.

    Article Snippet: For real time PCR, each 20-µL reaction mixture consisted of 5 µL of cDNA, 10 µL TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 µL of TaqMan Gene Ex Assays (Assay ID: Hs00210019_m1).

    Techniques: Western Blot, SDS Page

    Relationship of SAMHD1 transcription, Type I interferon response, and disease severity. There was a significant positive correlation of SAMHD1 transcript and level of MX1, an indicator of the Type I interferon response ( A ). Comparing Mx1 ( B ) and SAMHD1 ( C ) transcript with disease severity as assigned by pathology (none, mild, moderate, or severe), the association of Mx1 with disease appeared stronger than the association of SAMHD1 and disease. Transcript abundance was normalized to the average of beta actin and GAPDH; hence, the smaller the “dCq” value, the greater the abundance.

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: Relationship of SAMHD1 transcription, Type I interferon response, and disease severity. There was a significant positive correlation of SAMHD1 transcript and level of MX1, an indicator of the Type I interferon response ( A ). Comparing Mx1 ( B ) and SAMHD1 ( C ) transcript with disease severity as assigned by pathology (none, mild, moderate, or severe), the association of Mx1 with disease appeared stronger than the association of SAMHD1 and disease. Transcript abundance was normalized to the average of beta actin and GAPDH; hence, the smaller the “dCq” value, the greater the abundance.

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques:

    Brain SAMHD1 transcript load during SIV infection. SAMHD1 transcript increased in thalamus during SIV infection, with peak expression corresponding with acute and late-stage infection. SAMHD1 mRNA was quantitated by qPCR, with normalization to the average of beta actin and GAPDH. **p

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: Brain SAMHD1 transcript load during SIV infection. SAMHD1 transcript increased in thalamus during SIV infection, with peak expression corresponding with acute and late-stage infection. SAMHD1 mRNA was quantitated by qPCR, with normalization to the average of beta actin and GAPDH. **p

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction

    Viral load and SAMHD1. Tissue viral load as determined by standard curve-normalized qPCR did not correlate with SAMHD1 transcript (normalized to the average of beta actin and GAPDH).

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: Viral load and SAMHD1. Tissue viral load as determined by standard curve-normalized qPCR did not correlate with SAMHD1 transcript (normalized to the average of beta actin and GAPDH).

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques: Real-time Polymerase Chain Reaction

    SAMHD1 and IFNB1 in primary macrophages. M×1 of primary human monocyte-derived macrophages responded rapidly and strongly to 100 U/mL treatment with recombinant IFNB1, but not to TNFalpha (20 ng/mL, ( A )). SAMHD1 also responded to IFNB1, but the increase was detectable and significant only by 12 hours post-treatment ( B ). HIV-1 (BaL) infection of primary monocyte-derived macrophages yielded an apparent increase of SAMHD1 levels by 7 and 10 days post-infection, but this increase was not statistically significant (NS) ( C ). ***p

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: SAMHD1 and IFNB1 in primary macrophages. M×1 of primary human monocyte-derived macrophages responded rapidly and strongly to 100 U/mL treatment with recombinant IFNB1, but not to TNFalpha (20 ng/mL, ( A )). SAMHD1 also responded to IFNB1, but the increase was detectable and significant only by 12 hours post-treatment ( B ). HIV-1 (BaL) infection of primary monocyte-derived macrophages yielded an apparent increase of SAMHD1 levels by 7 and 10 days post-infection, but this increase was not statistically significant (NS) ( C ). ***p

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques: Derivative Assay, Recombinant, Infection

    Primary astrocyte SAMHD1 responds to SIV infection and IFNB1. Quantitative PCR results, normalized to the average of beta actin and GAPDH, indicated that SIV infection (SIV 17E-Fr) and IFNB1 treatment of primary rhesus astrocytes resulted in significant upregulation of SAMHD1 by 48 hours post infection and at all subsequent time points examined. SIV infection alone stimulated apparently significant upregulation at two and 14 days post infection. ***p

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: Primary astrocyte SAMHD1 responds to SIV infection and IFNB1. Quantitative PCR results, normalized to the average of beta actin and GAPDH, indicated that SIV infection (SIV 17E-Fr) and IFNB1 treatment of primary rhesus astrocytes resulted in significant upregulation of SAMHD1 by 48 hours post infection and at all subsequent time points examined. SIV infection alone stimulated apparently significant upregulation at two and 14 days post infection. ***p

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques: Infection, Real-time Polymerase Chain Reaction