Structured Review

Roche deoxynucleotide triphosphate
Deoxynucleotide Triphosphate, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleotide triphosphate/product/Roche
Average 92 stars, based on 23 article reviews
Price from $9.99 to $1999.99
deoxynucleotide triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Amplification:

Article Title: Bone Mass and the CAG and GGN Androgen Receptor Polymorphisms in Young Men
Article Snippet: .. Amplification was performed in a 25 µl reaction volume, containing 50 ng of genomic DNA, 200 µM of each deoxynucleotide triphosphate, 1x Fast Start Taq DNA polymerase Buffer (Roche Applied Science, Mannheim, Germany), 1x GC-rich solution buffer (Roche Applied Science) and 1U of Fast Start Taq DNA polymerase (Roche Applied Science). .. The concentration of each pair of primers was 1.2 and 1.5 µM for the amplification of the CAG and GGN repeats, respectively.

Isolation:

Article Title: Myeloid cell transmigration across the CNS vasculature triggers IL-1β–driven neuroinflammation during autoimmune encephalomyelitis in mice
Article Snippet: .. First-strand cDNA synthesis was accomplished using 0.2 µg of isolated RNA in a reaction containing 200 U SuperScript III RNase H-Reverse transcription (Thermo Fisher Scientific), 300 ng oligo-dT18 , 50 ng of random hexamers, 50 mM Tris-HCl, pH 8.3, 75 mM KCl, 3 mM MgCl2 , 0.5 mM deoxynucleotide triphosphate, 5 mM dithiothreitol, and 40 U Protector RNase inhibitor (Roche) in a final volume of 50 µl. .. The reaction was incubated at 25°C for 10 min and then at 50°C for 1 h, and a PCR purification kit (QIAGEN) was used to purify cDNA (Thermo Fisher Scientific).

Concentration Assay:

Article Title: Mutations in Bartonella bacilliformis gyrB Confer Resistance to Coumermycin A1
Article Snippet: .. Reaction mixtures contained 10 mM Tris-HCl (pH 8.3), 50 mM KCl, a 200 μM concentration of each deoxynucleotide triphosphate, 4 mM MgCl2 , 2.5 U of Ampli Taq DNA polymerase (Roche Molecular Systems, Branchburg, N.J.), 1 to 100 ng of template DNA, and 0.1 μg of each primer. ..

Incubation:

Article Title: Effect of piperlongumine on drug resistance reversal in human retinoblastoma HXO-RB44/VCR and SO-Rb50/CBP cell lines
Article Snippet: .. Total mRNA was then reverse transcribed for 1 hr at 42°C in incubation buffer containing 250 μM of each deoxynucleotide triphosphate, 5 μM oligo (dT) 20, 25 units of RNase inhibitor, and 20 units of avian myeloblastosis virus reverse transcriptase (Roche Diagnostics). .. The transcription level of target proteins was detected by semiquantitative real-time PCR using the icycler iQ detection system (Bio-Rad).

other:

Article Title: Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR
Article Snippet: Reverse transcription was initiated by the addition of 5× First Strand Buffer, 200 U of Superscript II (Invitrogen), 0.5 mM of each deoxynucleotide triphosphate (Roche Applied Science, Mannheim, Germany), with ultra-pure water (Fisher Biotech, Perth, Australia) to a final volume of 20 μl.

Polymerase Chain Reaction:

Article Title: Bone marrow cells participate in tumor vessel formation that supports the growth of Ewing's sarcoma in the lung
Article Snippet: .. The PCR conditions were 50-μL total volume with 200 ng of DNA as template, 0.1 μM primers, 0.4 mM deoxynucleotide triphosphate, 1× PCR buffer with magnesium, and 2.5 units of Taq DNA polymerase (Roche, Nutley, NJ). .. Four weeks after BM transplant, 2 × 106 of TC71-PM4 cells were intravenously injected into recipient nude mice.

Article Title: Serotyping and Genotyping of Genital Chlamydia trachomatis Isolates Reveal Variants of Serovars Ba, G, and J as Confirmed by omp1 Nucleotide Sequence Analysis
Article Snippet: .. The PCR mixture (final volume, 50 μl) contained 50 mM KCl, 1.5 mM MgCl2 , 10 mM Tris-HCl (pH 8.3), 200 μM (each) deoxynucleotide triphosphate (dATP, dTTP, dGTP, and dCTP), 50 pmol of each primer, and 1 U of Taq polymerase (Amplitaq; Roche Molecular Systems, Branchburg, N.Y.). ..

Article Title: The N34S mutation of SPINK1 (PSTI) is associated with a familial pattern of idiopathic chronic pancreatitis but does not cause the disease
Article Snippet: .. PCR was performed using 50 ng genomic DNA template, 0.5 U Pfu DNA polymerase (Promega), 2 mmol/l MgCl2 , 200 μmol/l each deoxynucleotide triphosphate (Roche Diagnostics), and 200 nmol/l of each primer in a 50 μl reaction volume. ..

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  • 85
    Roche deoxynucleotidyl transferase mediated deoxyuridine 5 triphosphate nick end labeling tunel assay
    Impact of glycyrrhizic acid treatment on hepatic apoptosis induced by carbon tetrachloride in rats. A: Liver tissue sections from the different groups were subjected to immunohistochemistry to determine the expression level of cleaved caspase-3 (original magnification, × 400); B: Fluorescence microscopy image showing terminal <t>deoxynucleotidyl</t> transferase-mediated deoxyuridine 5-triphosphate nick end labeling <t>(TUNEL)</t> stain (dashed arrows), and the same tissue slices were respectively counterstained with 4’6-diamidino-2-phenylindole (DAPI) to localize the nuclei (arrows). Images of combined with DAPI, indicated TUNEL-positive cells (arrow heads) (original magnification, × 200). GA: Glycyrrhizic acid; CCl 4 : Carbon tetrachloride.
    Deoxynucleotidyl Transferase Mediated Deoxyuridine 5 Triphosphate Nick End Labeling Tunel Assay, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotidyl transferase mediated deoxyuridine 5 triphosphate nick end labeling tunel assay/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotidyl transferase mediated deoxyuridine 5 triphosphate nick end labeling tunel assay - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    89
    Roche deoxynucleotidyl transferase tdt mediated deoxyuridine 5 triphosphate dutp nick end labeling tunel
    CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; <t>TUNEL,</t> terminal <t>deoxynucleotidyl</t> transferase <t>(TdT)-mediated</t> <t>dUTP</t> nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.
    Deoxynucleotidyl Transferase Tdt Mediated Deoxyuridine 5 Triphosphate Dutp Nick End Labeling Tunel, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotidyl transferase tdt mediated deoxyuridine 5 triphosphate dutp nick end labeling tunel/product/Roche
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotidyl transferase tdt mediated deoxyuridine 5 triphosphate dutp nick end labeling tunel - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    90
    Roche α 32p dctps
    CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; <t>TUNEL,</t> terminal <t>deoxynucleotidyl</t> transferase <t>(TdT)-mediated</t> <t>dUTP</t> nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.
    α 32p Dctps, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α 32p dctps/product/Roche
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α 32p dctps - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    92
    Roche deoxynucleotide triphosphate
    CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; <t>TUNEL,</t> terminal <t>deoxynucleotidyl</t> transferase <t>(TdT)-mediated</t> <t>dUTP</t> nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.
    Deoxynucleotide Triphosphate, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/Roche
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Impact of glycyrrhizic acid treatment on hepatic apoptosis induced by carbon tetrachloride in rats. A: Liver tissue sections from the different groups were subjected to immunohistochemistry to determine the expression level of cleaved caspase-3 (original magnification, × 400); B: Fluorescence microscopy image showing terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) stain (dashed arrows), and the same tissue slices were respectively counterstained with 4’6-diamidino-2-phenylindole (DAPI) to localize the nuclei (arrows). Images of combined with DAPI, indicated TUNEL-positive cells (arrow heads) (original magnification, × 200). GA: Glycyrrhizic acid; CCl 4 : Carbon tetrachloride.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Glycyrrhizic acid attenuates CCl4-induced hepatocyte apoptosis in rats via a p53-mediated pathway

    doi: 10.3748/wjg.v19.i24.3781

    Figure Lengend Snippet: Impact of glycyrrhizic acid treatment on hepatic apoptosis induced by carbon tetrachloride in rats. A: Liver tissue sections from the different groups were subjected to immunohistochemistry to determine the expression level of cleaved caspase-3 (original magnification, × 400); B: Fluorescence microscopy image showing terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) stain (dashed arrows), and the same tissue slices were respectively counterstained with 4’6-diamidino-2-phenylindole (DAPI) to localize the nuclei (arrows). Images of combined with DAPI, indicated TUNEL-positive cells (arrow heads) (original magnification, × 200). GA: Glycyrrhizic acid; CCl 4 : Carbon tetrachloride.

    Article Snippet: The terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) assay (Roche, Germany) was performed in accordance with the manufacturer’s protocol.

    Techniques: Immunohistochemistry, Expressing, Fluorescence, Microscopy, End Labeling, TUNEL Assay, Staining

    CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: International Journal of Nanomedicine

    Article Title: PEGylated apoptotic protein-loaded PLGA microspheres for cancer therapy

    doi: 10.2147/IJN.S75821

    Figure Lengend Snippet: CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: In vitro TUNEL assay in pancreatic Mia Paca-2 cells Apoptotic pancreatic Mia Paca-2 cells produced by TRAIL or PEG-TRAIL release (1, 3, and 7 days) from PLGA MSs were observed by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine 5′-triphosphate (dUTP) nick end labeling (TUNEL) using an in situ cell death detection kit (Fluorescein; Roche Diagnostics).

    Techniques: Confocal Laser Scanning Microscopy, Staining, Mass Spectrometry, TUNEL Assay, End Labeling

    Histologic observations of tumor tissues obtained from Mia Paca-2 cell-xenografted BALB/c nu/nu mice treated with blank, TRAIL, or PEG-TRAIL PLGA MSs for 27 days. The sections shown were stained with H E and TUNEL reagent (nuclei are stained blue and apoptotic cells red) (original magnifications: ×250). Red fluorescence represents apoptosis manifestation. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; PLGA MS, poly(lactic- co -glycolic acid) microsphere; H E, hematoxylin and eosin; TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling; dUTP, deoxyuridine 5′-triphosphate; TNF, tumor necrosis factor; PEG, polyethylene glycol.

    Journal: International Journal of Nanomedicine

    Article Title: PEGylated apoptotic protein-loaded PLGA microspheres for cancer therapy

    doi: 10.2147/IJN.S75821

    Figure Lengend Snippet: Histologic observations of tumor tissues obtained from Mia Paca-2 cell-xenografted BALB/c nu/nu mice treated with blank, TRAIL, or PEG-TRAIL PLGA MSs for 27 days. The sections shown were stained with H E and TUNEL reagent (nuclei are stained blue and apoptotic cells red) (original magnifications: ×250). Red fluorescence represents apoptosis manifestation. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; PLGA MS, poly(lactic- co -glycolic acid) microsphere; H E, hematoxylin and eosin; TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling; dUTP, deoxyuridine 5′-triphosphate; TNF, tumor necrosis factor; PEG, polyethylene glycol.

    Article Snippet: In vitro TUNEL assay in pancreatic Mia Paca-2 cells Apoptotic pancreatic Mia Paca-2 cells produced by TRAIL or PEG-TRAIL release (1, 3, and 7 days) from PLGA MSs were observed by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine 5′-triphosphate (dUTP) nick end labeling (TUNEL) using an in situ cell death detection kit (Fluorescein; Roche Diagnostics).

    Techniques: Mouse Assay, Staining, TUNEL Assay, Fluorescence, Mass Spectrometry, End Labeling