deoxynucleotide triphosphate  (Roche)


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    Structured Review

    Roche deoxynucleotide triphosphate
    Deoxynucleotide Triphosphate, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/Roche
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-09
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Global radiation in a rare biosphere soil diatom
    Article Snippet: .. The PCR mixtures contained per sample 1 µL of the template DNA, 0.4 µM of each primer, 200 µM of each deoxynucleotide triphosphate, 2.5 µL of 10x PCR buffer, and 0.25U of Fast Start High fidelity Taq polymerase (Roche Inc.). .. The final reaction volume was adjusted to 25 µL using HPLC water.

    Article Title: Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons.
    Article Snippet: .. The Tm distribution was adjusted for the frequency of each three-base combination for SNPs in the human genome. pcr Reaction conditions for the engineered plasmids and the -globin samples consisted of 50 mM Tris (pH 8.3), 500 g/mL bovine serum albumin, 3 mM MgCl2, 200 M each deoxynucleotide triphosphate, 0.4 U of Taq polymerase (Roche), 1 LCGreen I (Idaho Technology), and 0.5 M each primer in 10 L. The DNA templates were used at 106 copies (plasmids) or 20 ng (genomic), and a twotemperature PCR was performed with 35 cycles of 85 °C with no hold and 55 °C for 1 s on either the LightCycler (Roche) or the RapidCycler II (Idaho Technology). .. PCR was completed within 12 min. PCR for the prothrombin, factor V, MTHFR, and HFE targets was performed in a LightCycler with reagents ow nloaded from https://academ ic.oup.com /clinchem /article/50/7/1156/5640116 by guest on 16 August 2020 commonly used in clinical laboratories.

    Article Title: Isolates of Cryptococcus neoformans from Infected Animals Reveal Genetic Exchange in Unisexual, ? Mating Type Populations ▿ from Infected Animals Reveal Genetic Exchange in Unisexual, ? Mating Type Populations ▿ †
    Article Snippet: .. The amplification of the URA5 gene was performed with 50-μl reaction mixtures containing 1× PCR buffer, 0.125 mM deoxynucleotide triphosphate, 1.5 mM MgCl2 , 0.2 μM concentrations of each of the primers URA5 and SJO1, 2.5 U of Taq DNA polymerase (Roche), and 1 μl of template DNA. .. PCR amplification conditions were 94°C for 5 min followed by 35 cycles of 94°C for 45 s, 61°C for 1 min, and 72°C for 2 min, with a final extension step at 72°C for 7 min. PCR products were digested with Sau96I and HhaI for 3 h at 37°C and separated on 3% agarose gels by electrophoresis at 10 V/cm for 1.5 h. Standard strains of known molecular types were used as controls.

    Amplification:

    Article Title: Isolates of Cryptococcus neoformans from Infected Animals Reveal Genetic Exchange in Unisexual, ? Mating Type Populations ▿ from Infected Animals Reveal Genetic Exchange in Unisexual, ? Mating Type Populations ▿ †
    Article Snippet: .. The amplification of the URA5 gene was performed with 50-μl reaction mixtures containing 1× PCR buffer, 0.125 mM deoxynucleotide triphosphate, 1.5 mM MgCl2 , 0.2 μM concentrations of each of the primers URA5 and SJO1, 2.5 U of Taq DNA polymerase (Roche), and 1 μl of template DNA. .. PCR amplification conditions were 94°C for 5 min followed by 35 cycles of 94°C for 45 s, 61°C for 1 min, and 72°C for 2 min, with a final extension step at 72°C for 7 min. PCR products were digested with Sau96I and HhaI for 3 h at 37°C and separated on 3% agarose gels by electrophoresis at 10 V/cm for 1.5 h. Standard strains of known molecular types were used as controls.

    Article Title: Rhinovirus-Associated Hospitalizations in Young Children
    Article Snippet: .. Amplifications were performed by adding 0.5 µL of the primary amplification product to 49.5 µL of the reaction mixture containing 1 × reaction buffer with Mg++ , 0.2 mmol/L each deoxynucleotide triphosphate, 0.2 U of Taq DNA polymerase (Roche Diagnostics), and 0.2 µmol/L of each primer. .. Thermocycling was performed on a MicroAmp 9600 (Applied Biosystems) instrument set for 94°C denaturation for 2 min; 30 cycles of amplification at 94°C for 1 min, 58°C for 1 min, and 72°C for 1 min; and a final amplicon extension at 72°C for 5 min. Amplicons were analyzed by electrophoresis on 2% agarose gels with ethidium bromide staining, with a subset sequenced to confirm rhinovirus specificity.

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    Roche deoxynucleotidyl transferase mediated deoxyuridine 5 triphosphate nick end labeling tunel assay
    Impact of glycyrrhizic acid treatment on hepatic apoptosis induced by carbon tetrachloride in rats. A: Liver tissue sections from the different groups were subjected to immunohistochemistry to determine the expression level of cleaved caspase-3 (original magnification, × 400); B: Fluorescence microscopy image showing terminal <t>deoxynucleotidyl</t> transferase-mediated deoxyuridine 5-triphosphate nick end labeling <t>(TUNEL)</t> stain (dashed arrows), and the same tissue slices were respectively counterstained with 4’6-diamidino-2-phenylindole (DAPI) to localize the nuclei (arrows). Images of combined with DAPI, indicated TUNEL-positive cells (arrow heads) (original magnification, × 200). GA: Glycyrrhizic acid; CCl 4 : Carbon tetrachloride.
    Deoxynucleotidyl Transferase Mediated Deoxyuridine 5 Triphosphate Nick End Labeling Tunel Assay, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotidyl transferase mediated deoxyuridine 5 triphosphate nick end labeling tunel assay/product/Roche
    Average 85 stars, based on 1 article reviews
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    89
    Roche deoxynucleotidyl transferase tdt mediated deoxyuridine 5 triphosphate dutp nick end labeling tunel
    CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; <t>TUNEL,</t> terminal <t>deoxynucleotidyl</t> transferase <t>(TdT)-mediated</t> <t>dUTP</t> nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.
    Deoxynucleotidyl Transferase Tdt Mediated Deoxyuridine 5 Triphosphate Dutp Nick End Labeling Tunel, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotidyl transferase tdt mediated deoxyuridine 5 triphosphate dutp nick end labeling tunel/product/Roche
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
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    90
    Roche α 32p dctps
    CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; <t>TUNEL,</t> terminal <t>deoxynucleotidyl</t> transferase <t>(TdT)-mediated</t> <t>dUTP</t> nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.
    α 32p Dctps, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α 32p dctps/product/Roche
    Average 90 stars, based on 1 article reviews
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    α 32p dctps - by Bioz Stars, 2020-09
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    92
    Roche deoxynucleoside triphosphate
    CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; <t>TUNEL,</t> terminal <t>deoxynucleotidyl</t> transferase <t>(TdT)-mediated</t> <t>dUTP</t> nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.
    Deoxynucleoside Triphosphate, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/Roche
    Average 92 stars, based on 196 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Impact of glycyrrhizic acid treatment on hepatic apoptosis induced by carbon tetrachloride in rats. A: Liver tissue sections from the different groups were subjected to immunohistochemistry to determine the expression level of cleaved caspase-3 (original magnification, × 400); B: Fluorescence microscopy image showing terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) stain (dashed arrows), and the same tissue slices were respectively counterstained with 4’6-diamidino-2-phenylindole (DAPI) to localize the nuclei (arrows). Images of combined with DAPI, indicated TUNEL-positive cells (arrow heads) (original magnification, × 200). GA: Glycyrrhizic acid; CCl 4 : Carbon tetrachloride.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Glycyrrhizic acid attenuates CCl4-induced hepatocyte apoptosis in rats via a p53-mediated pathway

    doi: 10.3748/wjg.v19.i24.3781

    Figure Lengend Snippet: Impact of glycyrrhizic acid treatment on hepatic apoptosis induced by carbon tetrachloride in rats. A: Liver tissue sections from the different groups were subjected to immunohistochemistry to determine the expression level of cleaved caspase-3 (original magnification, × 400); B: Fluorescence microscopy image showing terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) stain (dashed arrows), and the same tissue slices were respectively counterstained with 4’6-diamidino-2-phenylindole (DAPI) to localize the nuclei (arrows). Images of combined with DAPI, indicated TUNEL-positive cells (arrow heads) (original magnification, × 200). GA: Glycyrrhizic acid; CCl 4 : Carbon tetrachloride.

    Article Snippet: The terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) assay (Roche, Germany) was performed in accordance with the manufacturer’s protocol.

    Techniques: Immunohistochemistry, Expressing, Fluorescence, Microscopy, End Labeling, TUNEL Assay, Staining

    CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: International Journal of Nanomedicine

    Article Title: PEGylated apoptotic protein-loaded PLGA microspheres for cancer therapy

    doi: 10.2147/IJN.S75821

    Figure Lengend Snippet: CLSM images of Mia Paca-2 cells. Notes: ( A ) CLSM images of untreated control Mia Paca-2 cells, and Mia Paca-2 cells treated with empty PLGA MSs, TRAIL, or PEG-TRAIL supernatants released from PLGA MSs at days 1, 3, and 5 (nuclei are stained blue and apoptotic cells green; original magnifications: ×400). Red arrows indicate late apoptosis manifestation. White arrows indicate no or negligible apoptosis. ( B ) Cell viability profiles of Mia Paca-2 cells treated with released TRAIL or PEG-TRAIL. Abbreviations: CLSM, confocal laser scanning microscopy; PLGA MS, poly(lactic- co -glycolic acid) microsphere; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; TNF, tumor necrosis factor; PEG, polyethylene glycol; TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling; dUTP, deoxyuridine 5′-triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: In vitro TUNEL assay in pancreatic Mia Paca-2 cells Apoptotic pancreatic Mia Paca-2 cells produced by TRAIL or PEG-TRAIL release (1, 3, and 7 days) from PLGA MSs were observed by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine 5′-triphosphate (dUTP) nick end labeling (TUNEL) using an in situ cell death detection kit (Fluorescein; Roche Diagnostics).

    Techniques: Confocal Laser Scanning Microscopy, Staining, Mass Spectrometry, TUNEL Assay, End Labeling

    Histologic observations of tumor tissues obtained from Mia Paca-2 cell-xenografted BALB/c nu/nu mice treated with blank, TRAIL, or PEG-TRAIL PLGA MSs for 27 days. The sections shown were stained with H E and TUNEL reagent (nuclei are stained blue and apoptotic cells red) (original magnifications: ×250). Red fluorescence represents apoptosis manifestation. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; PLGA MS, poly(lactic- co -glycolic acid) microsphere; H E, hematoxylin and eosin; TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling; dUTP, deoxyuridine 5′-triphosphate; TNF, tumor necrosis factor; PEG, polyethylene glycol.

    Journal: International Journal of Nanomedicine

    Article Title: PEGylated apoptotic protein-loaded PLGA microspheres for cancer therapy

    doi: 10.2147/IJN.S75821

    Figure Lengend Snippet: Histologic observations of tumor tissues obtained from Mia Paca-2 cell-xenografted BALB/c nu/nu mice treated with blank, TRAIL, or PEG-TRAIL PLGA MSs for 27 days. The sections shown were stained with H E and TUNEL reagent (nuclei are stained blue and apoptotic cells red) (original magnifications: ×250). Red fluorescence represents apoptosis manifestation. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; TRAIL, TNF-related apoptosis-inducing ligand; PEG-TRAIL, PEGylated TNF-related apoptosis-inducing ligand; PLGA MS, poly(lactic- co -glycolic acid) microsphere; H E, hematoxylin and eosin; TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling; dUTP, deoxyuridine 5′-triphosphate; TNF, tumor necrosis factor; PEG, polyethylene glycol.

    Article Snippet: In vitro TUNEL assay in pancreatic Mia Paca-2 cells Apoptotic pancreatic Mia Paca-2 cells produced by TRAIL or PEG-TRAIL release (1, 3, and 7 days) from PLGA MSs were observed by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine 5′-triphosphate (dUTP) nick end labeling (TUNEL) using an in situ cell death detection kit (Fluorescein; Roche Diagnostics).

    Techniques: Mouse Assay, Staining, TUNEL Assay, Fluorescence, Mass Spectrometry, End Labeling