deoxynucleoside triphosphate  (Thermo Fisher)


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    Structured Review

    Thermo Fisher deoxynucleoside triphosphate
    Deoxynucleoside Triphosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/Thermo Fisher
    Average 93 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-07
    93/100 stars

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    Polymerase Chain Reaction:

    Article Title: The Origin of Malarial Parasites in Orangutans
    Article Snippet: .. PCR was carried out in a 50 µl volume and included 20 ng/µl of total genomic DNA, 2.5 mM MgCl2 , 1× PCR buffer, 1.25 mM of each deoxynucleoside triphosphate, 0.4 mM of each primer, and 0.03 U/µM of AmpliTaq Gold® DNA polymerase (Applied Biosystems, Roche-USA). .. The primer forward 5′ GAC CAA GTA ACA ACG GGA G and reverse 5′ CAA AGA GTG GCT CAG AAC C were used to amplify the MSP142 gene, The PCR conditions were: a partial denaturation at 94°C for 4 min and 35 cycles of 1 min at 94°C, 1 min at 55°C, and 2 min extension at 72°C, and a final extension of 10 min was added in the last cycle.

    Article Title: Accelerated Diversification of Nonhuman Primate Malarias in Southeast Asia: Adaptive Radiation or Geographic Speciation?
    Article Snippet: .. The primary PCR amplifications were carried out in a 50 µl volume reaction using 20 ng of total genomic DNA, 3 mM MgCl2 , 1 × PCR buffer, 1.25 mM of each deoxynucleoside triphosphate, 0.4 mM of each primer, and 0.03 U/µl AmpliTaq polymerase (Applied Biosystems, Roche-USA). .. The primary PCR conditions were: A partial denaturation at 94 °C for 4 min and 36 cycles with 1 min at 94 °C, 1 min at 53 °C and 2 min extension at 72 °C, and a final extension of 10 min at 72 °C was added in the last cycle.

    Article Title: Single genome analysis reveals genetic characteristics of Neuroadaptation across HIV-1 envelope
    Article Snippet: .. PCR amplification was carried out in presence of 1x High Fidelity Platinum Taq PCR buffer, 2 mM MgSO4, 0.2 mM each deoxynucleoside triphosphate, 0.2 μM each primer, and 0.025 units/ μL of Platinum Taq High Fidelity polymerase in a 20 μL reaction (Invitrogen, Carlsbad, CA). ..

    Article Title: The Lower Serum Immunoglobulin G2 Level in Severe Cases than in Mild Cases of Pandemic H1N1 2009 Influenza Is Associated with Cytokine Dysregulation ▿
    Article Snippet: .. The PCR mixture (25 μl) contained denatured human genomic DNA, PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 2 mM MgCl2 , and 0.01% gelatin), 200 μM each deoxynucleoside triphosphate, and 0.625 U AmpliTaq gold polymerase (Applied Biosystems, Foster City, CA). .. Hot-start PCR was performed using the following conditions: 5 min at 95°C, 40 cycles of 95°C for 1 min, 50°C (58°C for primers F1/R1 and F3/R3) for 1 min, and 72°C for 90 s, with a final extension at 72°C for 10 min in an automated thermal cycler (Applied Biosystems, Foster City, CA).

    Article Title: Identifying Host Sources of Fecal Pollution: Diversity of Escherichia coli in Confined Dairy and Swine Production Systems
    Article Snippet: .. The final reaction mix (25μl) consisted of 1× PCR buffer (Promega, Madison, WI), 3 mM MgCl2 , 0.1 mg/ml gelatin, 200 μM of each deoxynucleoside triphosphate (Invitrogen, Burlington, Ontario, Canada), 2 μM each of forward and reverse primers ERIC-1 and ERIC, 1 U of Taq polymerase (Promega), and 2 μl of E. coli suspended cells as template. .. Amplification was performed in a Hybaid OmniGene thermocycler (InterSciences Inc., Markham, Ontario, Canada) as follows: after an initial denaturation at 95°C for 10 min, 34 cycles of denaturation (94°C, 3 seconds), (92°C, 30 seconds), annealing (50°C, 1 min), and extension (65°C, 1 min) were performed, followed by a final extension (65°C, 8 min).

    Amplification:

    Article Title: TNF-α -308 G > A and -238 G > A polymorphisms are not major risk factors in Caucasian patients with exfoliation glaucoma
    Article Snippet: .. DNA samples were amplified in 25 µl aliquots containing 200 µM deoxynucleoside triphosphate, 10 µM of each primer, 1.5 mM MgCl2 , 1 µl DNA sample, and 2 U Taq polymerase (Applied Biosystems, Foster City, CA). .. The PCR products were digested at 37 °C with NcoI to detect the SNP in the −308 gene allele and MspI to detect the polymorphism of the −238 nucleotide.

    Article Title: Single genome analysis reveals genetic characteristics of Neuroadaptation across HIV-1 envelope
    Article Snippet: .. PCR amplification was carried out in presence of 1x High Fidelity Platinum Taq PCR buffer, 2 mM MgSO4, 0.2 mM each deoxynucleoside triphosphate, 0.2 μM each primer, and 0.025 units/ μL of Platinum Taq High Fidelity polymerase in a 20 μL reaction (Invitrogen, Carlsbad, CA). ..

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    Thermo Fisher gene exp samhd1 hs00210019 m1
    Proposed model for p21-mediated HIV-1 restriction in MDDCs. The expression of p21 is induced during MDDC maturation (step 1). The increase in p21 expression leads to the downregulation of several enzymes (RNR2, TYMS, and TK1) involved in dNTP biosynthesis (step 2), which would decrease the dNTP pool size (step 3), impairing HIV-1 reverse transcription (step 4). In parallel, p21 induction could increase the active isoform of <t>SAMHD1</t> (step 5), which would further block viral replication through the degradation of dNTPs (step 6) or exonuclease activity (step 7). Low levels of dNTP synthesis as a consequence of p21 induction might further facilitate the exonuclease activity of SAMHD1 (step 8). Although not explored here, induction of p21 might also directly block reverse transcriptase activity and impair HIV transcription, as has been shown in CD4 + ).
    Gene Exp Samhd1 Hs00210019 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp samhd1 hs00210019 m1/product/Thermo Fisher
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    gene exp samhd1 hs00210019 m1 - by Bioz Stars, 2020-07
    94/100 stars
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    92
    Thermo Fisher deoxynucleotide triphosphate
    Proposed model for p21-mediated HIV-1 restriction in MDDCs. The expression of p21 is induced during MDDC maturation (step 1). The increase in p21 expression leads to the downregulation of several enzymes (RNR2, TYMS, and TK1) involved in dNTP biosynthesis (step 2), which would decrease the dNTP pool size (step 3), impairing HIV-1 reverse transcription (step 4). In parallel, p21 induction could increase the active isoform of <t>SAMHD1</t> (step 5), which would further block viral replication through the degradation of dNTPs (step 6) or exonuclease activity (step 7). Low levels of dNTP synthesis as a consequence of p21 induction might further facilitate the exonuclease activity of SAMHD1 (step 8). Although not explored here, induction of p21 might also directly block reverse transcriptase activity and impair HIV transcription, as has been shown in CD4 + ).
    Deoxynucleotide Triphosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/Thermo Fisher
    Average 92 stars, based on 340 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Proposed model for p21-mediated HIV-1 restriction in MDDCs. The expression of p21 is induced during MDDC maturation (step 1). The increase in p21 expression leads to the downregulation of several enzymes (RNR2, TYMS, and TK1) involved in dNTP biosynthesis (step 2), which would decrease the dNTP pool size (step 3), impairing HIV-1 reverse transcription (step 4). In parallel, p21 induction could increase the active isoform of SAMHD1 (step 5), which would further block viral replication through the degradation of dNTPs (step 6) or exonuclease activity (step 7). Low levels of dNTP synthesis as a consequence of p21 induction might further facilitate the exonuclease activity of SAMHD1 (step 8). Although not explored here, induction of p21 might also directly block reverse transcriptase activity and impair HIV transcription, as has been shown in CD4 + ).

    Journal: Journal of Virology

    Article Title: p21 Restricts HIV-1 in Monocyte-Derived Dendritic Cells through the Reduction of Deoxynucleoside Triphosphate Biosynthesis and Regulation of SAMHD1 Antiviral Activity

    doi: 10.1128/JVI.01324-17

    Figure Lengend Snippet: Proposed model for p21-mediated HIV-1 restriction in MDDCs. The expression of p21 is induced during MDDC maturation (step 1). The increase in p21 expression leads to the downregulation of several enzymes (RNR2, TYMS, and TK1) involved in dNTP biosynthesis (step 2), which would decrease the dNTP pool size (step 3), impairing HIV-1 reverse transcription (step 4). In parallel, p21 induction could increase the active isoform of SAMHD1 (step 5), which would further block viral replication through the degradation of dNTPs (step 6) or exonuclease activity (step 7). Low levels of dNTP synthesis as a consequence of p21 induction might further facilitate the exonuclease activity of SAMHD1 (step 8). Although not explored here, induction of p21 might also directly block reverse transcriptase activity and impair HIV transcription, as has been shown in CD4 + ).

    Article Snippet: PCR amplification of cDNAs was carried out as previously described ( ) on a 7500 real-time PCR system using the TaqMan gene expression premade mix (Applied Biosystems) to amplify the following genes: RRM2 (Hs00357247_g1), TYMS (Hs00426586_m1), SAMHD1 (Hs00210019_m1), CTPS1 (Hs01041858_m1), TK1 (Hs01062125_m1), CDKN1A (Hs00355782_m1), and GAPDH (Hs02758991_g1).

    Techniques: Expressing, Blocking Assay, Activity Assay

    p21 expression modulates SAMHD1 phosphorylation levels in MDDCs. (A to C) Relative copies of SAMHD1 mRNA (A), total SAMHD1 protein levels (B), and pSAMHD1 protein levels (C) in immature and mature MDDC after transfection with siRNA p21 or siRNA NT. The data are expressed relative to the number of mRNA copies or protein levels in immature MDDCs transfected with nontargeting siRNA. The symbols represent independent experiments ( n = 9 donors for mRNA and n = 4 donors for proteins). (D) Changes in luciferase activity after infection with HIV-1 NL4.3ΔenvLuc VSV-G in immature and mature MDDCs cultured in the presence of virus-like particles with or without the SIV VPX protein. The results are shown as the fold change relative to the luciferase activity in immature MDDCs cultured with VLPs not carrying VPX. The symbols represent independent experiments ( n = 9). The median and IQR values are shown. P values of ANOVA are shown. Significant differences ( P

    Journal: Journal of Virology

    Article Title: p21 Restricts HIV-1 in Monocyte-Derived Dendritic Cells through the Reduction of Deoxynucleoside Triphosphate Biosynthesis and Regulation of SAMHD1 Antiviral Activity

    doi: 10.1128/JVI.01324-17

    Figure Lengend Snippet: p21 expression modulates SAMHD1 phosphorylation levels in MDDCs. (A to C) Relative copies of SAMHD1 mRNA (A), total SAMHD1 protein levels (B), and pSAMHD1 protein levels (C) in immature and mature MDDC after transfection with siRNA p21 or siRNA NT. The data are expressed relative to the number of mRNA copies or protein levels in immature MDDCs transfected with nontargeting siRNA. The symbols represent independent experiments ( n = 9 donors for mRNA and n = 4 donors for proteins). (D) Changes in luciferase activity after infection with HIV-1 NL4.3ΔenvLuc VSV-G in immature and mature MDDCs cultured in the presence of virus-like particles with or without the SIV VPX protein. The results are shown as the fold change relative to the luciferase activity in immature MDDCs cultured with VLPs not carrying VPX. The symbols represent independent experiments ( n = 9). The median and IQR values are shown. P values of ANOVA are shown. Significant differences ( P

    Article Snippet: PCR amplification of cDNAs was carried out as previously described ( ) on a 7500 real-time PCR system using the TaqMan gene expression premade mix (Applied Biosystems) to amplify the following genes: RRM2 (Hs00357247_g1), TYMS (Hs00426586_m1), SAMHD1 (Hs00210019_m1), CTPS1 (Hs01041858_m1), TK1 (Hs01062125_m1), CDKN1A (Hs00355782_m1), and GAPDH (Hs02758991_g1).

    Techniques: Expressing, Transfection, Luciferase, Activity Assay, Infection, Cell Culture

    Phosphorylation state of SAMHD1. Monocytes (Monocyte), M-CSF-differentiated macrophages (Macrophage (M-CSF)), and GM-CSF-differentiated macrophages (Macrophage (GM-CSF)) were treated with GH123-Nhe/VSV-G for 2 h and incubated at 37°C for 4 h (4 h) or 24 h (24 h), or mock-treated (−). Cells were lyzed and subjected for SDS-PAGE containing Phos-tag to separate phosphorylated proteins from nonphosphorylated ones. SAMHD1 proteins were detected by anti-SAMHD1 antibody. Upper bands shown by a vertical bar and a lower band shown by an arrow represent phosphorylated and nonphosphorylated SAMHD1, respectively. Ratios of phosphorylated SAMHD1 levels to total SAMHD1 levels (ratio) are shown in vertical numbers.

    Journal: PLoS ONE

    Article Title: Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages

    doi: 10.1371/journal.pone.0090969

    Figure Lengend Snippet: Phosphorylation state of SAMHD1. Monocytes (Monocyte), M-CSF-differentiated macrophages (Macrophage (M-CSF)), and GM-CSF-differentiated macrophages (Macrophage (GM-CSF)) were treated with GH123-Nhe/VSV-G for 2 h and incubated at 37°C for 4 h (4 h) or 24 h (24 h), or mock-treated (−). Cells were lyzed and subjected for SDS-PAGE containing Phos-tag to separate phosphorylated proteins from nonphosphorylated ones. SAMHD1 proteins were detected by anti-SAMHD1 antibody. Upper bands shown by a vertical bar and a lower band shown by an arrow represent phosphorylated and nonphosphorylated SAMHD1, respectively. Ratios of phosphorylated SAMHD1 levels to total SAMHD1 levels (ratio) are shown in vertical numbers.

    Article Snippet: For real time PCR, each 20-µL reaction mixture consisted of 5 µL of cDNA, 10 µL TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 µL of TaqMan Gene Ex Assays (Assay ID: Hs00210019_m1).

    Techniques: Incubation, SDS Page

    Quantification of SAMHD1 mRNA expression. Monocytes were differentiated into macrophages for 6 days in the presence of GM-CSF or M-CSF. Macrophages or monocytes were treated with or without VSV-G-pseudotyped and Env-defective HIV-2 particles (GH123/VSV-G), and harvested. SAMHD1 mRNA expression levels were detected by real-time RT-PCR and normalized against GAPDH. Data are shown as mean ± SD.

    Journal: PLoS ONE

    Article Title: Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages

    doi: 10.1371/journal.pone.0090969

    Figure Lengend Snippet: Quantification of SAMHD1 mRNA expression. Monocytes were differentiated into macrophages for 6 days in the presence of GM-CSF or M-CSF. Macrophages or monocytes were treated with or without VSV-G-pseudotyped and Env-defective HIV-2 particles (GH123/VSV-G), and harvested. SAMHD1 mRNA expression levels were detected by real-time RT-PCR and normalized against GAPDH. Data are shown as mean ± SD.

    Article Snippet: For real time PCR, each 20-µL reaction mixture consisted of 5 µL of cDNA, 10 µL TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 µL of TaqMan Gene Ex Assays (Assay ID: Hs00210019_m1).

    Techniques: Expressing, Quantitative RT-PCR

    Effects of SAMHD1 siRNA on HIV-1 infection. Monocytes (Monocyte) or macrophages (Macrophage) were treated with SAMHD1- (siSAMHD1) or GFP- (siGFP) targeting siRNAs. Three days after transfection, cells were harvested or infected with NL43-Luci/VSV-G virus. SAMHD1 mRNA expression levels in harvested cells were detected by real-time RT-PCR and normalized against GAPDH. Data are shown as mean ± SD. Luciferase activity in infected cells was measured 4 days after infection. Data are plotted as the mean ± SD of triplicate samples; presented data are representative of two independent experiments using two donors. A: Results of samples obtained from a donor 1. B: Results of samples obtained from a donor 2.

    Journal: PLoS ONE

    Article Title: Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages

    doi: 10.1371/journal.pone.0090969

    Figure Lengend Snippet: Effects of SAMHD1 siRNA on HIV-1 infection. Monocytes (Monocyte) or macrophages (Macrophage) were treated with SAMHD1- (siSAMHD1) or GFP- (siGFP) targeting siRNAs. Three days after transfection, cells were harvested or infected with NL43-Luci/VSV-G virus. SAMHD1 mRNA expression levels in harvested cells were detected by real-time RT-PCR and normalized against GAPDH. Data are shown as mean ± SD. Luciferase activity in infected cells was measured 4 days after infection. Data are plotted as the mean ± SD of triplicate samples; presented data are representative of two independent experiments using two donors. A: Results of samples obtained from a donor 1. B: Results of samples obtained from a donor 2.

    Article Snippet: For real time PCR, each 20-µL reaction mixture consisted of 5 µL of cDNA, 10 µL TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 µL of TaqMan Gene Ex Assays (Assay ID: Hs00210019_m1).

    Techniques: Infection, Transfection, Expressing, Quantitative RT-PCR, Luciferase, Activity Assay

    Western blot analysis of SAMHD1 in undifferentiated monocytes and macrophages. Monocytes were differentiated into macrophages for 6 days in the presence of GM-CSF or M-CSF. Macrophages or monocytes were treated with or without VSV-G-pseudotyped and Env-defective HIV-2 particles (GH123/VSV-G), and harvested. Whole-cell extracts were separated on SDS-PAGE and analyzed by western blot using the indicated antibodies. Presented data are representative of two independent experiments.

    Journal: PLoS ONE

    Article Title: Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages

    doi: 10.1371/journal.pone.0090969

    Figure Lengend Snippet: Western blot analysis of SAMHD1 in undifferentiated monocytes and macrophages. Monocytes were differentiated into macrophages for 6 days in the presence of GM-CSF or M-CSF. Macrophages or monocytes were treated with or without VSV-G-pseudotyped and Env-defective HIV-2 particles (GH123/VSV-G), and harvested. Whole-cell extracts were separated on SDS-PAGE and analyzed by western blot using the indicated antibodies. Presented data are representative of two independent experiments.

    Article Snippet: For real time PCR, each 20-µL reaction mixture consisted of 5 µL of cDNA, 10 µL TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 µL of TaqMan Gene Ex Assays (Assay ID: Hs00210019_m1).

    Techniques: Western Blot, SDS Page

    Relationship of SAMHD1 transcription, Type I interferon response, and disease severity. There was a significant positive correlation of SAMHD1 transcript and level of MX1, an indicator of the Type I interferon response ( A ). Comparing Mx1 ( B ) and SAMHD1 ( C ) transcript with disease severity as assigned by pathology (none, mild, moderate, or severe), the association of Mx1 with disease appeared stronger than the association of SAMHD1 and disease. Transcript abundance was normalized to the average of beta actin and GAPDH; hence, the smaller the “dCq” value, the greater the abundance.

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: Relationship of SAMHD1 transcription, Type I interferon response, and disease severity. There was a significant positive correlation of SAMHD1 transcript and level of MX1, an indicator of the Type I interferon response ( A ). Comparing Mx1 ( B ) and SAMHD1 ( C ) transcript with disease severity as assigned by pathology (none, mild, moderate, or severe), the association of Mx1 with disease appeared stronger than the association of SAMHD1 and disease. Transcript abundance was normalized to the average of beta actin and GAPDH; hence, the smaller the “dCq” value, the greater the abundance.

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques:

    Brain SAMHD1 transcript load during SIV infection. SAMHD1 transcript increased in thalamus during SIV infection, with peak expression corresponding with acute and late-stage infection. SAMHD1 mRNA was quantitated by qPCR, with normalization to the average of beta actin and GAPDH. **p

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: Brain SAMHD1 transcript load during SIV infection. SAMHD1 transcript increased in thalamus during SIV infection, with peak expression corresponding with acute and late-stage infection. SAMHD1 mRNA was quantitated by qPCR, with normalization to the average of beta actin and GAPDH. **p

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction

    Viral load and SAMHD1. Tissue viral load as determined by standard curve-normalized qPCR did not correlate with SAMHD1 transcript (normalized to the average of beta actin and GAPDH).

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: Viral load and SAMHD1. Tissue viral load as determined by standard curve-normalized qPCR did not correlate with SAMHD1 transcript (normalized to the average of beta actin and GAPDH).

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques: Real-time Polymerase Chain Reaction

    SAMHD1 and IFNB1 in primary macrophages. M×1 of primary human monocyte-derived macrophages responded rapidly and strongly to 100 U/mL treatment with recombinant IFNB1, but not to TNFalpha (20 ng/mL, ( A )). SAMHD1 also responded to IFNB1, but the increase was detectable and significant only by 12 hours post-treatment ( B ). HIV-1 (BaL) infection of primary monocyte-derived macrophages yielded an apparent increase of SAMHD1 levels by 7 and 10 days post-infection, but this increase was not statistically significant (NS) ( C ). ***p

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: SAMHD1 and IFNB1 in primary macrophages. M×1 of primary human monocyte-derived macrophages responded rapidly and strongly to 100 U/mL treatment with recombinant IFNB1, but not to TNFalpha (20 ng/mL, ( A )). SAMHD1 also responded to IFNB1, but the increase was detectable and significant only by 12 hours post-treatment ( B ). HIV-1 (BaL) infection of primary monocyte-derived macrophages yielded an apparent increase of SAMHD1 levels by 7 and 10 days post-infection, but this increase was not statistically significant (NS) ( C ). ***p

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques: Derivative Assay, Recombinant, Infection

    Primary astrocyte SAMHD1 responds to SIV infection and IFNB1. Quantitative PCR results, normalized to the average of beta actin and GAPDH, indicated that SIV infection (SIV 17E-Fr) and IFNB1 treatment of primary rhesus astrocytes resulted in significant upregulation of SAMHD1 by 48 hours post infection and at all subsequent time points examined. SIV infection alone stimulated apparently significant upregulation at two and 14 days post infection. ***p

    Journal: Scientific Reports

    Article Title: SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

    doi: 10.1038/srep22629

    Figure Lengend Snippet: Primary astrocyte SAMHD1 responds to SIV infection and IFNB1. Quantitative PCR results, normalized to the average of beta actin and GAPDH, indicated that SIV infection (SIV 17E-Fr) and IFNB1 treatment of primary rhesus astrocytes resulted in significant upregulation of SAMHD1 by 48 hours post infection and at all subsequent time points examined. SIV infection alone stimulated apparently significant upregulation at two and 14 days post infection. ***p

    Article Snippet: Pre-designed TaqMan Assays (Applied Biosystems/Life Technologies) were used to quantitate SAMHD1, Mx1, GAPDH, and ACTB from human (Hs00210019_m1, Hs00895608_m1, Hs02758991_g1, and Hs99999903_m1, respectively) and macaque (Rh02869978_m1, Rh02842279_m1, Rh02621745_g1, and Rh03043379_gH, respectively).

    Techniques: Infection, Real-time Polymerase Chain Reaction