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Promega deoxynucleoside triphosphate
RT-PCR amplification of SLE virus SL-33. Lane M, Marker VIII (Boehringer-Mannheim, Mannheim, Germany); lane 1, PCR product obtained by the standard protocol (with each <t>deoxynucleoside</t> triphosphate at a concentration of 200 μM); lane 2, PCR product obtained by the BESS T-scan protocol (each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP).
Deoxynucleoside Triphosphate, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Promega
Average 92 stars, based on 162 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "Use of Base Excision Sequence Scanning for Detection of Genetic Variations in St. Louis Encephalitis Virus Isolates"

Article Title: Use of Base Excision Sequence Scanning for Detection of Genetic Variations in St. Louis Encephalitis Virus Isolates

Journal: Journal of Clinical Microbiology

doi:

RT-PCR amplification of SLE virus SL-33. Lane M, Marker VIII (Boehringer-Mannheim, Mannheim, Germany); lane 1, PCR product obtained by the standard protocol (with each deoxynucleoside triphosphate at a concentration of 200 μM); lane 2, PCR product obtained by the BESS T-scan protocol (each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP).
Figure Legend Snippet: RT-PCR amplification of SLE virus SL-33. Lane M, Marker VIII (Boehringer-Mannheim, Mannheim, Germany); lane 1, PCR product obtained by the standard protocol (with each deoxynucleoside triphosphate at a concentration of 200 μM); lane 2, PCR product obtained by the BESS T-scan protocol (each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP).

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Amplification, Marker, Polymerase Chain Reaction, Concentration Assay

Related Articles

Amplification:

Article Title: Use of Base Excision Sequence Scanning for Detection of Genetic Variations in St. Louis Encephalitis Virus Isolates
Article Snippet: .. Two microliters of a 1:50 dilution of the purified PCR product was used as the target DNA for amplification on a PTC-100 thermocycler (MJ Research) in a 25-μl reaction mixture containing 2.5 μl of 10× PCR buffer (Promega), 1.5 mM (final concentration) MgCl2 , 2 μl of BESS T-scan dNTP Mix with each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP (Epicentre, Madison, Wis.), 1.25 U of Taq DNA polymerase (Promega), 3 pmol of 6-carboxyfluorescein (6-FAM)-labeled forward primer (Perkin-Elmer, Foster City, Calif.), and 3 pmol of a reverse primer. .. All products of the reverse transcription-PCRs were revealed by UV transillumination after electrophoresis on a 1.2% agarose gel containing ethidium bromide to verify that only the specific band had been amplified.

Article Title: Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR †
Article Snippet: .. PCRs were carried out in 25-μl mixtures containing 1× Taq buffer, 1.5 mM MgCl2 , 0.04 μM primer J-RXg , 0.04 μM primer J-RXc2 , each deoxynucleoside triphosphate at a concentration of 0.2 mM, and 1 U of Taq polymerase (Promega); the PCR amplification conditions were the same as those used with the other primers. .. The products were visualized under UV light in 2% agarose gels stained with ethidium bromide.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Amplification of a Complete Simian Immunodeficiency Virus Genome from Fecal RNA of a Wild Chimpanzee
Article Snippet: .. For cDNA synthesis, 10 μl of extracted fecal RNA was added to an RT-PCR master mix consisting of 1× buffer II (Perkin-Elmer, La Jolla, Calif.), 5 mM MgCl2 , 1 mM deoxynucleoside triphosphate, 5 mM dithiothreitol, 20 pmol of reverse primer (Table ), 20 U of RNase inhibitor (Promega, Madison, Wis.), and 100 U of Superscript RT II (Gibco-BRL, Rockville, Md.) and incubated for 1 h at 42°C. .. Ten microliters of this cDNA preparation was then added to a PCR mix consisting of Expand Buffer 2 (Roche Molecular Biochemicals, Indianapolis, Ind.), 0.35 mM deoxynucleoside triphosphate, 2.25 mM MgCl2 , 0.1 μg of bovine serum albumin/ml, 2.5 U of Expand high-fidelity Taq polymerase (Roche Molecular Biochemicals), and 10 pmol of forward and reverse primers (Table ) and subjected to nested (or seminested) PCR amplifications.

Concentration Assay:

Article Title: Use of Base Excision Sequence Scanning for Detection of Genetic Variations in St. Louis Encephalitis Virus Isolates
Article Snippet: .. Two microliters of a 1:50 dilution of the purified PCR product was used as the target DNA for amplification on a PTC-100 thermocycler (MJ Research) in a 25-μl reaction mixture containing 2.5 μl of 10× PCR buffer (Promega), 1.5 mM (final concentration) MgCl2 , 2 μl of BESS T-scan dNTP Mix with each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP (Epicentre, Madison, Wis.), 1.25 U of Taq DNA polymerase (Promega), 3 pmol of 6-carboxyfluorescein (6-FAM)-labeled forward primer (Perkin-Elmer, Foster City, Calif.), and 3 pmol of a reverse primer. .. All products of the reverse transcription-PCRs were revealed by UV transillumination after electrophoresis on a 1.2% agarose gel containing ethidium bromide to verify that only the specific band had been amplified.

Article Title: Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR †
Article Snippet: .. PCRs were carried out in 25-μl mixtures containing 1× Taq buffer, 1.5 mM MgCl2 , 0.04 μM primer J-RXg , 0.04 μM primer J-RXc2 , each deoxynucleoside triphosphate at a concentration of 0.2 mM, and 1 U of Taq polymerase (Promega); the PCR amplification conditions were the same as those used with the other primers. .. The products were visualized under UV light in 2% agarose gels stained with ethidium bromide.

Article Title: Molecular, Antigenic, and Functional Characteristics of Ferric Enterobactin Receptor CfrA in Campylobacter jejuni ▿
Article Snippet: .. Each PCR was performed with a 50-μl mixture containing each deoxynucleoside triphosphate at a concentration of 200 μM, each primer at a concentration of 200 nM, 2.5 mM MgSO4 , 50 ng of template DNA, and 5 U of Taq DNA polymerase (Promega) or PfuUltra high-fidelity DNA polymerase (Stratagene). ..

Incubation:

Article Title: Amplification of a Complete Simian Immunodeficiency Virus Genome from Fecal RNA of a Wild Chimpanzee
Article Snippet: .. For cDNA synthesis, 10 μl of extracted fecal RNA was added to an RT-PCR master mix consisting of 1× buffer II (Perkin-Elmer, La Jolla, Calif.), 5 mM MgCl2 , 1 mM deoxynucleoside triphosphate, 5 mM dithiothreitol, 20 pmol of reverse primer (Table ), 20 U of RNase inhibitor (Promega, Madison, Wis.), and 100 U of Superscript RT II (Gibco-BRL, Rockville, Md.) and incubated for 1 h at 42°C. .. Ten microliters of this cDNA preparation was then added to a PCR mix consisting of Expand Buffer 2 (Roche Molecular Biochemicals, Indianapolis, Ind.), 0.35 mM deoxynucleoside triphosphate, 2.25 mM MgCl2 , 0.1 μg of bovine serum albumin/ml, 2.5 U of Expand high-fidelity Taq polymerase (Roche Molecular Biochemicals), and 10 pmol of forward and reverse primers (Table ) and subjected to nested (or seminested) PCR amplifications.

Polymerase Chain Reaction:

Article Title: Use of Base Excision Sequence Scanning for Detection of Genetic Variations in St. Louis Encephalitis Virus Isolates
Article Snippet: .. Two microliters of a 1:50 dilution of the purified PCR product was used as the target DNA for amplification on a PTC-100 thermocycler (MJ Research) in a 25-μl reaction mixture containing 2.5 μl of 10× PCR buffer (Promega), 1.5 mM (final concentration) MgCl2 , 2 μl of BESS T-scan dNTP Mix with each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP (Epicentre, Madison, Wis.), 1.25 U of Taq DNA polymerase (Promega), 3 pmol of 6-carboxyfluorescein (6-FAM)-labeled forward primer (Perkin-Elmer, Foster City, Calif.), and 3 pmol of a reverse primer. .. All products of the reverse transcription-PCRs were revealed by UV transillumination after electrophoresis on a 1.2% agarose gel containing ethidium bromide to verify that only the specific band had been amplified.

Article Title: Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR †
Article Snippet: .. PCRs were carried out in 25-μl mixtures containing 1× Taq buffer, 1.5 mM MgCl2 , 0.04 μM primer J-RXg , 0.04 μM primer J-RXc2 , each deoxynucleoside triphosphate at a concentration of 0.2 mM, and 1 U of Taq polymerase (Promega); the PCR amplification conditions were the same as those used with the other primers. .. The products were visualized under UV light in 2% agarose gels stained with ethidium bromide.

Article Title: Clonal Characterization of Staphylococcus aureus by Multilocus Restriction Fragment Typing, a Rapid Screening Approach for Molecular Epidemiology
Article Snippet: .. PCR amplifications were done in 15-μl reaction volumes containing 1.0 μl of the boiled whole-cell lysate, 3.75 pmol of each of the forward and reverse primers, 200 μM each deoxynucleoside triphosphate, 0.1 mg of acetylated bovine serum albumin per ml, 0.75 U of Taq polymerase, and 1.5 μl of 10× buffer B with 1.5 mM MgCl2 supplied with the polymerase (Promega Corporation, Madison, Wis.). .. The primers and PCR cycling conditions used for MLRFT are the same as those described by Enright et al. ( ) and are updated on the S. aureus ).

Article Title: Molecular, Antigenic, and Functional Characteristics of Ferric Enterobactin Receptor CfrA in Campylobacter jejuni ▿
Article Snippet: .. Each PCR was performed with a 50-μl mixture containing each deoxynucleoside triphosphate at a concentration of 200 μM, each primer at a concentration of 200 nM, 2.5 mM MgSO4 , 50 ng of template DNA, and 5 U of Taq DNA polymerase (Promega) or PfuUltra high-fidelity DNA polymerase (Stratagene). ..

Activated Clotting Time Assay:

Article Title: Discovery and Phylogenetic Analysis of Novel Members of Class b Enterotoxigenic Escherichia coli Adhesive Fimbriae ▿
Article Snippet: .. Both simplex PCRs were performed in a 50-μl reaction mixture containing 1.2 μM dCS20F (5′-KCC AGY CTW TGC CAR GT-3′)/dCS20R (5′-YAC AGT ACC DGC YKT AAC-3′) and dCS20F/CS12R (5′-ATA GTC ATT ACT GCA TTT GCA TCA AC-3′), 10 μl of 5× Green Go Taq Flexi buffer, 2 mM MgCl2 , 100 μM each deoxynucleoside triphosphate, 2.5 U of Go Taq Flexi DNA polymerase (Promega, Madison, WI), and 5 μl of bacterial whole-cell lysate. .. DNA was denatured at 95°C for 5 min, followed by 30 cycles of denaturation at 95°C for 1 min, annealing at 44°C (dCS20F and dCS20R) or 54°C (dCS20F and CS12R) for 30 s, and extension of amplified products at 72°C for 1 min, with a final extension time of 7 min at 72°C.

Purification:

Article Title: Use of Base Excision Sequence Scanning for Detection of Genetic Variations in St. Louis Encephalitis Virus Isolates
Article Snippet: .. Two microliters of a 1:50 dilution of the purified PCR product was used as the target DNA for amplification on a PTC-100 thermocycler (MJ Research) in a 25-μl reaction mixture containing 2.5 μl of 10× PCR buffer (Promega), 1.5 mM (final concentration) MgCl2 , 2 μl of BESS T-scan dNTP Mix with each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP (Epicentre, Madison, Wis.), 1.25 U of Taq DNA polymerase (Promega), 3 pmol of 6-carboxyfluorescein (6-FAM)-labeled forward primer (Perkin-Elmer, Foster City, Calif.), and 3 pmol of a reverse primer. .. All products of the reverse transcription-PCRs were revealed by UV transillumination after electrophoresis on a 1.2% agarose gel containing ethidium bromide to verify that only the specific band had been amplified.

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  • 92
    Promega deoxynucleoside triphosphate
    RT-PCR amplification of SLE virus SL-33. Lane M, Marker VIII (Boehringer-Mannheim, Mannheim, Germany); lane 1, PCR product obtained by the standard protocol (with each <t>deoxynucleoside</t> triphosphate at a concentration of 200 μM); lane 2, PCR product obtained by the BESS T-scan protocol (each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP).
    Deoxynucleoside Triphosphate, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/Promega
    Average 92 stars, based on 162 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Promega deoxynucleoside triphosphate dntp
    RT-PCR amplification of SLE virus SL-33. Lane M, Marker VIII (Boehringer-Mannheim, Mannheim, Germany); lane 1, PCR product obtained by the standard protocol (with each <t>deoxynucleoside</t> triphosphate at a concentration of 200 μM); lane 2, PCR product obtained by the BESS T-scan protocol (each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP).
    Deoxynucleoside Triphosphate Dntp, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate dntp/product/Promega
    Average 93 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate dntp - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    85
    Promega deoxyonucleoside triphosphate mixture
    RT-PCR amplification of SLE virus SL-33. Lane M, Marker VIII (Boehringer-Mannheim, Mannheim, Germany); lane 1, PCR product obtained by the standard protocol (with each <t>deoxynucleoside</t> triphosphate at a concentration of 200 μM); lane 2, PCR product obtained by the BESS T-scan protocol (each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP).
    Deoxyonucleoside Triphosphate Mixture, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyonucleoside triphosphate mixture/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxyonucleoside triphosphate mixture - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    RT-PCR amplification of SLE virus SL-33. Lane M, Marker VIII (Boehringer-Mannheim, Mannheim, Germany); lane 1, PCR product obtained by the standard protocol (with each deoxynucleoside triphosphate at a concentration of 200 μM); lane 2, PCR product obtained by the BESS T-scan protocol (each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP).

    Journal: Journal of Clinical Microbiology

    Article Title: Use of Base Excision Sequence Scanning for Detection of Genetic Variations in St. Louis Encephalitis Virus Isolates

    doi:

    Figure Lengend Snippet: RT-PCR amplification of SLE virus SL-33. Lane M, Marker VIII (Boehringer-Mannheim, Mannheim, Germany); lane 1, PCR product obtained by the standard protocol (with each deoxynucleoside triphosphate at a concentration of 200 μM); lane 2, PCR product obtained by the BESS T-scan protocol (each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP).

    Article Snippet: Two microliters of a 1:50 dilution of the purified PCR product was used as the target DNA for amplification on a PTC-100 thermocycler (MJ Research) in a 25-μl reaction mixture containing 2.5 μl of 10× PCR buffer (Promega), 1.5 mM (final concentration) MgCl2 , 2 μl of BESS T-scan dNTP Mix with each deoxynucleoside triphosphate at a concentration of 2.5 mM and 200 μM dUTP (Epicentre, Madison, Wis.), 1.25 U of Taq DNA polymerase (Promega), 3 pmol of 6-carboxyfluorescein (6-FAM)-labeled forward primer (Perkin-Elmer, Foster City, Calif.), and 3 pmol of a reverse primer.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Marker, Polymerase Chain Reaction, Concentration Assay