Structured Review

FUJIFILM deoxycholic acid
Selected reaction monitoring (SRM) chromatograms of mixture of 18 bile acid standards. The standard mixture was labeled with either 2PA or 2PA-d6, and 1:1 mixture of the labeled compounds was analyzed by RPLC triple-quadrupole mass spectrometry. LCA: lithocholic acid, UDCA: ursodeoxycholic acid, HDCA: hyodeoxycholic acid, CDCA: chenodeoxycholic acid, DCA: <t>deoxycholic</t> acid, wMCA: ω-muricholic acid, aMCA: α-muricholic acid, bMCA: β-muricholic acid, HCA: hyocholic acid, CA: cholic acid, GLCA: glycolithocholic acid, GUDCA: glycoursodeoxycholic acid, GHDCA: glycohyodeoxycholic acid, GCDCA: glycochenodeoxycholic acid, GDCA: glycodeoxycholic acid, GaMCA: glyco-α-muricholic acid, GbMCA: glyco-β-muricholic acid, GHCA: glycohyocholic acid, GCA: glycocholic acid.
Deoxycholic Acid, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxycholic acid/product/FUJIFILM
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deoxycholic acid - by Bioz Stars, 2021-03
86/100 stars

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1) Product Images from "Comprehensive and semi-quantitative analysis of carboxyl-containing metabolites related to gut microbiota on chronic kidney disease using 2-picolylamine isotopic labeling LC-MS/MS"

Article Title: Comprehensive and semi-quantitative analysis of carboxyl-containing metabolites related to gut microbiota on chronic kidney disease using 2-picolylamine isotopic labeling LC-MS/MS

Journal: Scientific Reports

doi: 10.1038/s41598-019-55600-1

Selected reaction monitoring (SRM) chromatograms of mixture of 18 bile acid standards. The standard mixture was labeled with either 2PA or 2PA-d6, and 1:1 mixture of the labeled compounds was analyzed by RPLC triple-quadrupole mass spectrometry. LCA: lithocholic acid, UDCA: ursodeoxycholic acid, HDCA: hyodeoxycholic acid, CDCA: chenodeoxycholic acid, DCA: deoxycholic acid, wMCA: ω-muricholic acid, aMCA: α-muricholic acid, bMCA: β-muricholic acid, HCA: hyocholic acid, CA: cholic acid, GLCA: glycolithocholic acid, GUDCA: glycoursodeoxycholic acid, GHDCA: glycohyodeoxycholic acid, GCDCA: glycochenodeoxycholic acid, GDCA: glycodeoxycholic acid, GaMCA: glyco-α-muricholic acid, GbMCA: glyco-β-muricholic acid, GHCA: glycohyocholic acid, GCA: glycocholic acid.
Figure Legend Snippet: Selected reaction monitoring (SRM) chromatograms of mixture of 18 bile acid standards. The standard mixture was labeled with either 2PA or 2PA-d6, and 1:1 mixture of the labeled compounds was analyzed by RPLC triple-quadrupole mass spectrometry. LCA: lithocholic acid, UDCA: ursodeoxycholic acid, HDCA: hyodeoxycholic acid, CDCA: chenodeoxycholic acid, DCA: deoxycholic acid, wMCA: ω-muricholic acid, aMCA: α-muricholic acid, bMCA: β-muricholic acid, HCA: hyocholic acid, CA: cholic acid, GLCA: glycolithocholic acid, GUDCA: glycoursodeoxycholic acid, GHDCA: glycohyodeoxycholic acid, GCDCA: glycochenodeoxycholic acid, GDCA: glycodeoxycholic acid, GaMCA: glyco-α-muricholic acid, GbMCA: glyco-β-muricholic acid, GHCA: glycohyocholic acid, GCA: glycocholic acid.

Techniques Used: Labeling, Mass Spectrometry, High Content Screening

Related Articles

Blocking Assay:

Article Title: Reactive oxygen species involved cancer cellular specific 5-aminolevulinic acid uptake in gastric epithelial cells
Article Snippet: .. Ltd., Osaka, Japan), Triton X-100 (Sigma-Aldrich Japan K.K.), sodium dodecyl sulfate (SDS) (Wako), deoxycholic acid (Wako), hydrochloric acid (Wako), NuPAGE® Novex® 12% Bis-Tris gels (Life Technologies Japan Ltd., Tokyo, Japan), PVDF Blocking Reagent for Can Get Signal® (TOYOBO CO., LTD., Osaka, Japan), Tris Buffered Saline with Tween® 20 (TBST-10X, Cell Signaling Technology Japan, K.K., Tokyo, Japan), PEPT1 antibody (Abcam plc., Cambridge, U.K.), Can Get Signal® Immunoreaction Enhancer Solution 1 (TOYOBO) horseradish peroxidase (HRP) linked anti-rabbit IgG antibody (Cell Signaling Technology Japan, K.K.), Lumina forte western HRP substrate (Millipore Co., Billerica, MA), anti-β-actin (Cell Signaling), and Can Get Signal® Immunoreaction Enhancer Solution 2 (TOYOBO) were purchased and used without further purification or modification. .. Cell culture RGM1 and RGK1 were cultured in DMEM/F12 with L-glutamine (Life Technologies Japan Ltd.) and DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.), respectively.

Article Title: Nitric oxide regulates the expression of heme carrier protein-1 via hypoxia inducible factor-1α stabilization
Article Snippet: In this study, we determined whether the increase in NO production upregulated the expression of HCP-1 to enhance the PDT effect by stabilizing the expression of HIF-1α. .. Materials L-arginine, 2-methoxyestradiol (2-Me), deoxycholic acid, hydrochloric acid, and NaOH (Wako Pure Chemicals, Osaka, Japan); N6-(1-iminoethyl)-L-lysine (L-NIL) (Cayman, Michigan, USA); cell counting kit-8 (Dojindo, Tokyo, Japan); DAF-2DA (Daiichi Pure Chemicals, Tokyo, Japan); hematoporphyrin dihydrochloride (HpD), NaCl, sodium dodecyl sulfate (SDS), Trizma base, Triton X-100, and Tween 20 (Sigma-Aldrich Japan K.K., Tokyo, Japan); NuPAGE Novex 12% Bis-Tris gels (Life Technologies Japan, Tokyo, Japan); polyvinylidene difluoride (PVDF) Blocking Reagent for Can Get Signal, Can Get Signal Immunoreaction Enhancer Solution 1, and Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo, Osaka, Japan); HIF-1α, β-actin, and horseradish peroxidase (HRP)-linked anti-rabbit IgG antibodies (Cell Signaling Technology Japan, K.K., Tokyo, Japan); HCP-1 antibody (Abcam, Cambridge, U.K.); iNOS antibody (BiossAntibodies, Massachusetts, U.S.A.) and Lumina Forte western HRP substrate (EMD Millipore, Billerica, MA, USA) were purchased and used without further purification or modification. .. Cell culture The rat cancerous gastric mucosa cell line RGK36 , which is established in our laboratory [ ], was cultured in DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.).

Western Blot:

Article Title: Reactive oxygen species involved cancer cellular specific 5-aminolevulinic acid uptake in gastric epithelial cells
Article Snippet: .. Ltd., Osaka, Japan), Triton X-100 (Sigma-Aldrich Japan K.K.), sodium dodecyl sulfate (SDS) (Wako), deoxycholic acid (Wako), hydrochloric acid (Wako), NuPAGE® Novex® 12% Bis-Tris gels (Life Technologies Japan Ltd., Tokyo, Japan), PVDF Blocking Reagent for Can Get Signal® (TOYOBO CO., LTD., Osaka, Japan), Tris Buffered Saline with Tween® 20 (TBST-10X, Cell Signaling Technology Japan, K.K., Tokyo, Japan), PEPT1 antibody (Abcam plc., Cambridge, U.K.), Can Get Signal® Immunoreaction Enhancer Solution 1 (TOYOBO) horseradish peroxidase (HRP) linked anti-rabbit IgG antibody (Cell Signaling Technology Japan, K.K.), Lumina forte western HRP substrate (Millipore Co., Billerica, MA), anti-β-actin (Cell Signaling), and Can Get Signal® Immunoreaction Enhancer Solution 2 (TOYOBO) were purchased and used without further purification or modification. .. Cell culture RGM1 and RGK1 were cultured in DMEM/F12 with L-glutamine (Life Technologies Japan Ltd.) and DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.), respectively.

Article Title: Nitric oxide regulates the expression of heme carrier protein-1 via hypoxia inducible factor-1α stabilization
Article Snippet: In this study, we determined whether the increase in NO production upregulated the expression of HCP-1 to enhance the PDT effect by stabilizing the expression of HIF-1α. .. Materials L-arginine, 2-methoxyestradiol (2-Me), deoxycholic acid, hydrochloric acid, and NaOH (Wako Pure Chemicals, Osaka, Japan); N6-(1-iminoethyl)-L-lysine (L-NIL) (Cayman, Michigan, USA); cell counting kit-8 (Dojindo, Tokyo, Japan); DAF-2DA (Daiichi Pure Chemicals, Tokyo, Japan); hematoporphyrin dihydrochloride (HpD), NaCl, sodium dodecyl sulfate (SDS), Trizma base, Triton X-100, and Tween 20 (Sigma-Aldrich Japan K.K., Tokyo, Japan); NuPAGE Novex 12% Bis-Tris gels (Life Technologies Japan, Tokyo, Japan); polyvinylidene difluoride (PVDF) Blocking Reagent for Can Get Signal, Can Get Signal Immunoreaction Enhancer Solution 1, and Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo, Osaka, Japan); HIF-1α, β-actin, and horseradish peroxidase (HRP)-linked anti-rabbit IgG antibodies (Cell Signaling Technology Japan, K.K., Tokyo, Japan); HCP-1 antibody (Abcam, Cambridge, U.K.); iNOS antibody (BiossAntibodies, Massachusetts, U.S.A.) and Lumina Forte western HRP substrate (EMD Millipore, Billerica, MA, USA) were purchased and used without further purification or modification. .. Cell culture The rat cancerous gastric mucosa cell line RGK36 , which is established in our laboratory [ ], was cultured in DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.).

Purification:

Article Title: Reactive oxygen species involved cancer cellular specific 5-aminolevulinic acid uptake in gastric epithelial cells
Article Snippet: .. Ltd., Osaka, Japan), Triton X-100 (Sigma-Aldrich Japan K.K.), sodium dodecyl sulfate (SDS) (Wako), deoxycholic acid (Wako), hydrochloric acid (Wako), NuPAGE® Novex® 12% Bis-Tris gels (Life Technologies Japan Ltd., Tokyo, Japan), PVDF Blocking Reagent for Can Get Signal® (TOYOBO CO., LTD., Osaka, Japan), Tris Buffered Saline with Tween® 20 (TBST-10X, Cell Signaling Technology Japan, K.K., Tokyo, Japan), PEPT1 antibody (Abcam plc., Cambridge, U.K.), Can Get Signal® Immunoreaction Enhancer Solution 1 (TOYOBO) horseradish peroxidase (HRP) linked anti-rabbit IgG antibody (Cell Signaling Technology Japan, K.K.), Lumina forte western HRP substrate (Millipore Co., Billerica, MA), anti-β-actin (Cell Signaling), and Can Get Signal® Immunoreaction Enhancer Solution 2 (TOYOBO) were purchased and used without further purification or modification. .. Cell culture RGM1 and RGK1 were cultured in DMEM/F12 with L-glutamine (Life Technologies Japan Ltd.) and DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.), respectively.

Article Title: Nitric oxide regulates the expression of heme carrier protein-1 via hypoxia inducible factor-1α stabilization
Article Snippet: In this study, we determined whether the increase in NO production upregulated the expression of HCP-1 to enhance the PDT effect by stabilizing the expression of HIF-1α. .. Materials L-arginine, 2-methoxyestradiol (2-Me), deoxycholic acid, hydrochloric acid, and NaOH (Wako Pure Chemicals, Osaka, Japan); N6-(1-iminoethyl)-L-lysine (L-NIL) (Cayman, Michigan, USA); cell counting kit-8 (Dojindo, Tokyo, Japan); DAF-2DA (Daiichi Pure Chemicals, Tokyo, Japan); hematoporphyrin dihydrochloride (HpD), NaCl, sodium dodecyl sulfate (SDS), Trizma base, Triton X-100, and Tween 20 (Sigma-Aldrich Japan K.K., Tokyo, Japan); NuPAGE Novex 12% Bis-Tris gels (Life Technologies Japan, Tokyo, Japan); polyvinylidene difluoride (PVDF) Blocking Reagent for Can Get Signal, Can Get Signal Immunoreaction Enhancer Solution 1, and Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo, Osaka, Japan); HIF-1α, β-actin, and horseradish peroxidase (HRP)-linked anti-rabbit IgG antibodies (Cell Signaling Technology Japan, K.K., Tokyo, Japan); HCP-1 antibody (Abcam, Cambridge, U.K.); iNOS antibody (BiossAntibodies, Massachusetts, U.S.A.) and Lumina Forte western HRP substrate (EMD Millipore, Billerica, MA, USA) were purchased and used without further purification or modification. .. Cell culture The rat cancerous gastric mucosa cell line RGK36 , which is established in our laboratory [ ], was cultured in DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.).

Modification:

Article Title: Reactive oxygen species involved cancer cellular specific 5-aminolevulinic acid uptake in gastric epithelial cells
Article Snippet: .. Ltd., Osaka, Japan), Triton X-100 (Sigma-Aldrich Japan K.K.), sodium dodecyl sulfate (SDS) (Wako), deoxycholic acid (Wako), hydrochloric acid (Wako), NuPAGE® Novex® 12% Bis-Tris gels (Life Technologies Japan Ltd., Tokyo, Japan), PVDF Blocking Reagent for Can Get Signal® (TOYOBO CO., LTD., Osaka, Japan), Tris Buffered Saline with Tween® 20 (TBST-10X, Cell Signaling Technology Japan, K.K., Tokyo, Japan), PEPT1 antibody (Abcam plc., Cambridge, U.K.), Can Get Signal® Immunoreaction Enhancer Solution 1 (TOYOBO) horseradish peroxidase (HRP) linked anti-rabbit IgG antibody (Cell Signaling Technology Japan, K.K.), Lumina forte western HRP substrate (Millipore Co., Billerica, MA), anti-β-actin (Cell Signaling), and Can Get Signal® Immunoreaction Enhancer Solution 2 (TOYOBO) were purchased and used without further purification or modification. .. Cell culture RGM1 and RGK1 were cultured in DMEM/F12 with L-glutamine (Life Technologies Japan Ltd.) and DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.), respectively.

Article Title: Nitric oxide regulates the expression of heme carrier protein-1 via hypoxia inducible factor-1α stabilization
Article Snippet: In this study, we determined whether the increase in NO production upregulated the expression of HCP-1 to enhance the PDT effect by stabilizing the expression of HIF-1α. .. Materials L-arginine, 2-methoxyestradiol (2-Me), deoxycholic acid, hydrochloric acid, and NaOH (Wako Pure Chemicals, Osaka, Japan); N6-(1-iminoethyl)-L-lysine (L-NIL) (Cayman, Michigan, USA); cell counting kit-8 (Dojindo, Tokyo, Japan); DAF-2DA (Daiichi Pure Chemicals, Tokyo, Japan); hematoporphyrin dihydrochloride (HpD), NaCl, sodium dodecyl sulfate (SDS), Trizma base, Triton X-100, and Tween 20 (Sigma-Aldrich Japan K.K., Tokyo, Japan); NuPAGE Novex 12% Bis-Tris gels (Life Technologies Japan, Tokyo, Japan); polyvinylidene difluoride (PVDF) Blocking Reagent for Can Get Signal, Can Get Signal Immunoreaction Enhancer Solution 1, and Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo, Osaka, Japan); HIF-1α, β-actin, and horseradish peroxidase (HRP)-linked anti-rabbit IgG antibodies (Cell Signaling Technology Japan, K.K., Tokyo, Japan); HCP-1 antibody (Abcam, Cambridge, U.K.); iNOS antibody (BiossAntibodies, Massachusetts, U.S.A.) and Lumina Forte western HRP substrate (EMD Millipore, Billerica, MA, USA) were purchased and used without further purification or modification. .. Cell culture The rat cancerous gastric mucosa cell line RGK36 , which is established in our laboratory [ ], was cultured in DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.).

CCK-8 Assay:

Article Title: Nitric oxide regulates the expression of heme carrier protein-1 via hypoxia inducible factor-1α stabilization
Article Snippet: In this study, we determined whether the increase in NO production upregulated the expression of HCP-1 to enhance the PDT effect by stabilizing the expression of HIF-1α. .. Materials L-arginine, 2-methoxyestradiol (2-Me), deoxycholic acid, hydrochloric acid, and NaOH (Wako Pure Chemicals, Osaka, Japan); N6-(1-iminoethyl)-L-lysine (L-NIL) (Cayman, Michigan, USA); cell counting kit-8 (Dojindo, Tokyo, Japan); DAF-2DA (Daiichi Pure Chemicals, Tokyo, Japan); hematoporphyrin dihydrochloride (HpD), NaCl, sodium dodecyl sulfate (SDS), Trizma base, Triton X-100, and Tween 20 (Sigma-Aldrich Japan K.K., Tokyo, Japan); NuPAGE Novex 12% Bis-Tris gels (Life Technologies Japan, Tokyo, Japan); polyvinylidene difluoride (PVDF) Blocking Reagent for Can Get Signal, Can Get Signal Immunoreaction Enhancer Solution 1, and Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo, Osaka, Japan); HIF-1α, β-actin, and horseradish peroxidase (HRP)-linked anti-rabbit IgG antibodies (Cell Signaling Technology Japan, K.K., Tokyo, Japan); HCP-1 antibody (Abcam, Cambridge, U.K.); iNOS antibody (BiossAntibodies, Massachusetts, U.S.A.) and Lumina Forte western HRP substrate (EMD Millipore, Billerica, MA, USA) were purchased and used without further purification or modification. .. Cell culture The rat cancerous gastric mucosa cell line RGK36 , which is established in our laboratory [ ], was cultured in DMEM/F12 without L-glutamine (Sigma-Aldrich Japan K.K.).

Incubation:

Article Title: Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells
Article Snippet: In some experiments, cells were treated with MG132 (Calbiochem) or recombinant IFN-β (PBL Assay Science) for 12 h. In BrdU incorporation analysis, cells were treated with 20μM BrdU (BD Pharmingen) for 16 h . .. HSCs were incubated with deoxycholic acid (Wako) for 6 days to induce cellular senescence and then LTA (InvivoGen) for 6 h before harvest . ..

Article Title: A BET family protein degrader provokes senolysis by targeting NHEJ and autophagy in senescent cells
Article Snippet: In vitro senescence induction For the induction of oncogene-induced senescence, IMR-90 ER:RAS cells were cultured with 4-OHT for 7days and TIG-3 cells infected with retroviruses encoding RasV12 were cultured 10 days after infection . .. For the induction of doxorubicin-induced senescence, TIG-3, hRPE, HCT116, and MEF cells were incubated with doxorubicin (DXR, cat#: 046-21523, FUJIFILM Wako Chemicals, 250, 100, 200, and 100 ng/ml, respectively) for 10 days. mHSC cells were treated with 275 μM deoxycholic acid (DCA, cat#: 046-18811, FUJIFILM Wako Chemicals) for 10 days. .. Cell proliferation assay Cells were seeded in 12-well plates.

Concentration Assay:

Article Title: NleC, a Type III Secretion Protease, Compromises NF-?B Activation by Targeting p65/RelA
Article Snippet: The bacterial cultures were separated into the cell pellet (whole cell) and the culture supernatant (supernatant) fractions by centrifugation at 8,000 rpm. .. The supernatant was filtered (pore size, 0.22 µM) and concentrated by the addition of trichloroacetic acid (Sigma) and deoxycholic acid (Wako) to a final concentration of 6% and 0.05%, respectively. .. The precipitates were re-dissolved in acetone (Wako) and centrifuged.

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  • 99
    FUJIFILM sodium deoxycholate
    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
    Sodium Deoxycholate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium deoxycholate/product/FUJIFILM
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium deoxycholate - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    86
    FUJIFILM na deoxycholate
    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
    Na Deoxycholate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na deoxycholate/product/FUJIFILM
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    na deoxycholate - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium deoxycholate complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.

    Journal: International Journal of Molecular Sciences

    Article Title: Multifunctional Composite Microcapsules for Oral Delivery of Insulin

    doi: 10.3390/ijms18010054

    Figure Lengend Snippet: Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium deoxycholate complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.

    Article Snippet: Sodium deoxycholate and poly(lactic-co -glycolic acid) (PLGA 75/25, molecular weight of 20 kDa) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).

    Techniques: