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Nacalai deoxycholic acid sodium salt monohydrate
CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or <t>DLPC/deoxycholic</t> acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.
Deoxycholic Acid Sodium Salt Monohydrate, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxycholic acid sodium salt monohydrate/product/Nacalai
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deoxycholic acid sodium salt monohydrate - by Bioz Stars, 2021-04
86/100 stars

Images

1) Product Images from "pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity"

Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

Journal: Vaccines

doi: 10.3390/vaccines5040041

CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or DLPC/deoxycholic acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.
Figure Legend Snippet: CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or DLPC/deoxycholic acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.

Techniques Used: Confocal Laser Scanning Microscopy, Staining

Confocal laser scanning microscopic (CLSM) images of DC2.4 cells treated with DLPC liposomes ( a ), EYPC/deoxycholic acid micelles ( b ) and DLPC/deoxycholic acid micelles ( c ) for 5 h. Fluorescence of NBD-PE and Rh-PE upon excitation at 488 nm was observed using a CLSM. Scale bar represents 10 μm.
Figure Legend Snippet: Confocal laser scanning microscopic (CLSM) images of DC2.4 cells treated with DLPC liposomes ( a ), EYPC/deoxycholic acid micelles ( b ) and DLPC/deoxycholic acid micelles ( c ) for 5 h. Fluorescence of NBD-PE and Rh-PE upon excitation at 488 nm was observed using a CLSM. Scale bar represents 10 μm.

Techniques Used: Confocal Laser Scanning Microscopy, Fluorescence

pH-responsive properties of DLPC/deoxycholic acid micelles. ( a ) Time courses of pyranine fluorescence for pyranine-loaded EYPC liposomes after addition of DLPC/deoxycholic acid micelles at pH 7.4 (red) or 5.0 (blue). Triton-X100 was added to dissociate the liposomal membrane completely. ( b ) Comparison of membrane disruptive activity for pyranine-loaded EYPC liposomes by addition of DOPE/CHEMS (green), DOPE/oleic acid (red) and DLPC/deoxycholic acid micelles (blue) at various pH. ( c ) Lipid mixing assay using EYPC liposomes containing Rh-PE/NBD-PE and various vehicles. Ratios of fluorescence intensity at 530 nm and 580 nm, which indicate a decrease of FRET by lipid mixing, are shown as a function of pH. Methanol was used to achieve complete lipid mixing.
Figure Legend Snippet: pH-responsive properties of DLPC/deoxycholic acid micelles. ( a ) Time courses of pyranine fluorescence for pyranine-loaded EYPC liposomes after addition of DLPC/deoxycholic acid micelles at pH 7.4 (red) or 5.0 (blue). Triton-X100 was added to dissociate the liposomal membrane completely. ( b ) Comparison of membrane disruptive activity for pyranine-loaded EYPC liposomes by addition of DOPE/CHEMS (green), DOPE/oleic acid (red) and DLPC/deoxycholic acid micelles (blue) at various pH. ( c ) Lipid mixing assay using EYPC liposomes containing Rh-PE/NBD-PE and various vehicles. Ratios of fluorescence intensity at 530 nm and 580 nm, which indicate a decrease of FRET by lipid mixing, are shown as a function of pH. Methanol was used to achieve complete lipid mixing.

Techniques Used: Fluorescence, Activity Assay

Interaction of DLPC/deoxycholic acid micelles with DC2.4 cells. ( a ) Effects of temperature for cellular association of micelles. DC2.4 cells were treated with Rh-PE-labeled micelles for 5 h at various temperatures. Cellular fluorescence was measured using a flow cytometer. ( b – d ) Effect of inhibitors for cellular association of micelles. Cellular association of Rh-PE-labeled micelles in the presence of various amounts of indicating inhibitors was evaluated. * p
Figure Legend Snippet: Interaction of DLPC/deoxycholic acid micelles with DC2.4 cells. ( a ) Effects of temperature for cellular association of micelles. DC2.4 cells were treated with Rh-PE-labeled micelles for 5 h at various temperatures. Cellular fluorescence was measured using a flow cytometer. ( b – d ) Effect of inhibitors for cellular association of micelles. Cellular association of Rh-PE-labeled micelles in the presence of various amounts of indicating inhibitors was evaluated. * p

Techniques Used: Labeling, Fluorescence, Flow Cytometry, Cytometry

Induction of cellular immunity by pH-responsive micelles. C57BL/6 mice were immunized intradermally with 16 μg of OVA, OVA + CpG-ODN, or OVA + DLPC/deoxycholic acid micelle or OVA + CpG-ODN + DLPC/deoxycholic acid micelle. Splenocytes (2 × 10 6 cells) were collected from the immunized mice on day 7 after immunization and were cultured with ( a ) or without ( b ) OVA CTL epitope peptide (SIINFEKL) for 40 h. ( a , b ) Typical micrographs of IFN-γ-producing spots measured by ELIspot. The number of IFN-γ-producing spots is shown ( c ).
Figure Legend Snippet: Induction of cellular immunity by pH-responsive micelles. C57BL/6 mice were immunized intradermally with 16 μg of OVA, OVA + CpG-ODN, or OVA + DLPC/deoxycholic acid micelle or OVA + CpG-ODN + DLPC/deoxycholic acid micelle. Splenocytes (2 × 10 6 cells) were collected from the immunized mice on day 7 after immunization and were cultured with ( a ) or without ( b ) OVA CTL epitope peptide (SIINFEKL) for 40 h. ( a , b ) Typical micrographs of IFN-γ-producing spots measured by ELIspot. The number of IFN-γ-producing spots is shown ( c ).

Techniques Used: Mouse Assay, Cell Culture, CTL Assay, Enzyme-linked Immunospot

Design of pH-responsive micelles composed of DLPC and deoxycholic acid for antigen delivery into cytosol of dendritic cells and induction of antigen presentation via MHC class I molecules (cross-presentation), which leads to antigen-specific cellular immunity. An atomic force microscope (AFM) image for DLPC/deoyxcholic acid micelles is also shown.
Figure Legend Snippet: Design of pH-responsive micelles composed of DLPC and deoxycholic acid for antigen delivery into cytosol of dendritic cells and induction of antigen presentation via MHC class I molecules (cross-presentation), which leads to antigen-specific cellular immunity. An atomic force microscope (AFM) image for DLPC/deoyxcholic acid micelles is also shown.

Techniques Used: Microscopy

Related Articles

other:

Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity
Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

Irradiation:

Article Title: A Low-Level Carbon Dioxide Laser Promotes Fibroblast Proliferation and Migration through Activation of Akt, ERK, and JNK
Article Snippet: Western blot analysis HDFs were plated at a density of 2.0 × 105 cells per 100mm dish, incubated in DMEM containing 10% FBS for 24 h, and then incubated in DMEM containing 1.0% FBS for 24 h. Confluent HDFs were washed twice with PBS, which was aspirated before laser irradiation, and then irradiated with the laser at 1.0 J/cm2 . .. At specified time points after irradiation (5, 15, or 30 min), HDFs were lysed in RIPA buffer, which contained 100 mM Tris, 150 mM NaCl, 0.1% sodium dodecyl sulfate, 0.5% deoxycholic acid sodium salt monohydrate (Nacalai Tesque, Kyoto, Japan), and 1.0% Nonidet P-40 (Wako, Osaka, Japan), incubated for 20 min at 4°C, and centrifuged at 15,000 × g for 15 min at 4°C. .. The proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto nitrocellulose membrane using an iBlot 2 Dry Blotting System (Invitrogen, MA, USA).

Incubation:

Article Title: A Low-Level Carbon Dioxide Laser Promotes Fibroblast Proliferation and Migration through Activation of Akt, ERK, and JNK
Article Snippet: Western blot analysis HDFs were plated at a density of 2.0 × 105 cells per 100mm dish, incubated in DMEM containing 10% FBS for 24 h, and then incubated in DMEM containing 1.0% FBS for 24 h. Confluent HDFs were washed twice with PBS, which was aspirated before laser irradiation, and then irradiated with the laser at 1.0 J/cm2 . .. At specified time points after irradiation (5, 15, or 30 min), HDFs were lysed in RIPA buffer, which contained 100 mM Tris, 150 mM NaCl, 0.1% sodium dodecyl sulfate, 0.5% deoxycholic acid sodium salt monohydrate (Nacalai Tesque, Kyoto, Japan), and 1.0% Nonidet P-40 (Wako, Osaka, Japan), incubated for 20 min at 4°C, and centrifuged at 15,000 × g for 15 min at 4°C. .. The proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto nitrocellulose membrane using an iBlot 2 Dry Blotting System (Invitrogen, MA, USA).

Lysis:

Article Title: Musashi-1 Post-Transcriptionally Enhances Phosphotyrosine-Binding Domain-Containing m-Numb Protein Expression in Regenerating Gastric Mucosa
Article Snippet: Polysome Gradient Fractionation From Gastric Tissues Preparation of gastric tissue for polysome analysis was performed essentially according to previously described methods . .. A 20-mg piece of solid tissue was lysed in 1 ml of lysis buffer (10 mM Tris-HCl at pH 8.0, 150 mM NaCl, 5 mM MgCl2 , 1% Nonidet-P40, 40 mM dithiothreitol, 1000 U/ml RNAase inhibitor (Toyobo), 40 mM vanadyl ribosyl complex (New England Bio Labs, Ipswich, MA, USA) supplemented with 1% deoxycholic acid sodium salt monohydrate (Nacalai Tesque, Inc., Kyoto, Japan). ..

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    Nacalai deoxycholic acid sodium salt monohydrate
    CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or <t>DLPC/deoxycholic</t> acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.
    Deoxycholic Acid Sodium Salt Monohydrate, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholic acid sodium salt monohydrate/product/Nacalai
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxycholic acid sodium salt monohydrate - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or DLPC/deoxycholic acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or DLPC/deoxycholic acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Confocal Laser Scanning Microscopy, Staining

    Confocal laser scanning microscopic (CLSM) images of DC2.4 cells treated with DLPC liposomes ( a ), EYPC/deoxycholic acid micelles ( b ) and DLPC/deoxycholic acid micelles ( c ) for 5 h. Fluorescence of NBD-PE and Rh-PE upon excitation at 488 nm was observed using a CLSM. Scale bar represents 10 μm.

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: Confocal laser scanning microscopic (CLSM) images of DC2.4 cells treated with DLPC liposomes ( a ), EYPC/deoxycholic acid micelles ( b ) and DLPC/deoxycholic acid micelles ( c ) for 5 h. Fluorescence of NBD-PE and Rh-PE upon excitation at 488 nm was observed using a CLSM. Scale bar represents 10 μm.

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Confocal Laser Scanning Microscopy, Fluorescence

    pH-responsive properties of DLPC/deoxycholic acid micelles. ( a ) Time courses of pyranine fluorescence for pyranine-loaded EYPC liposomes after addition of DLPC/deoxycholic acid micelles at pH 7.4 (red) or 5.0 (blue). Triton-X100 was added to dissociate the liposomal membrane completely. ( b ) Comparison of membrane disruptive activity for pyranine-loaded EYPC liposomes by addition of DOPE/CHEMS (green), DOPE/oleic acid (red) and DLPC/deoxycholic acid micelles (blue) at various pH. ( c ) Lipid mixing assay using EYPC liposomes containing Rh-PE/NBD-PE and various vehicles. Ratios of fluorescence intensity at 530 nm and 580 nm, which indicate a decrease of FRET by lipid mixing, are shown as a function of pH. Methanol was used to achieve complete lipid mixing.

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: pH-responsive properties of DLPC/deoxycholic acid micelles. ( a ) Time courses of pyranine fluorescence for pyranine-loaded EYPC liposomes after addition of DLPC/deoxycholic acid micelles at pH 7.4 (red) or 5.0 (blue). Triton-X100 was added to dissociate the liposomal membrane completely. ( b ) Comparison of membrane disruptive activity for pyranine-loaded EYPC liposomes by addition of DOPE/CHEMS (green), DOPE/oleic acid (red) and DLPC/deoxycholic acid micelles (blue) at various pH. ( c ) Lipid mixing assay using EYPC liposomes containing Rh-PE/NBD-PE and various vehicles. Ratios of fluorescence intensity at 530 nm and 580 nm, which indicate a decrease of FRET by lipid mixing, are shown as a function of pH. Methanol was used to achieve complete lipid mixing.

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Fluorescence, Activity Assay

    Interaction of DLPC/deoxycholic acid micelles with DC2.4 cells. ( a ) Effects of temperature for cellular association of micelles. DC2.4 cells were treated with Rh-PE-labeled micelles for 5 h at various temperatures. Cellular fluorescence was measured using a flow cytometer. ( b – d ) Effect of inhibitors for cellular association of micelles. Cellular association of Rh-PE-labeled micelles in the presence of various amounts of indicating inhibitors was evaluated. * p

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: Interaction of DLPC/deoxycholic acid micelles with DC2.4 cells. ( a ) Effects of temperature for cellular association of micelles. DC2.4 cells were treated with Rh-PE-labeled micelles for 5 h at various temperatures. Cellular fluorescence was measured using a flow cytometer. ( b – d ) Effect of inhibitors for cellular association of micelles. Cellular association of Rh-PE-labeled micelles in the presence of various amounts of indicating inhibitors was evaluated. * p

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Labeling, Fluorescence, Flow Cytometry, Cytometry

    Induction of cellular immunity by pH-responsive micelles. C57BL/6 mice were immunized intradermally with 16 μg of OVA, OVA + CpG-ODN, or OVA + DLPC/deoxycholic acid micelle or OVA + CpG-ODN + DLPC/deoxycholic acid micelle. Splenocytes (2 × 10 6 cells) were collected from the immunized mice on day 7 after immunization and were cultured with ( a ) or without ( b ) OVA CTL epitope peptide (SIINFEKL) for 40 h. ( a , b ) Typical micrographs of IFN-γ-producing spots measured by ELIspot. The number of IFN-γ-producing spots is shown ( c ).

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: Induction of cellular immunity by pH-responsive micelles. C57BL/6 mice were immunized intradermally with 16 μg of OVA, OVA + CpG-ODN, or OVA + DLPC/deoxycholic acid micelle or OVA + CpG-ODN + DLPC/deoxycholic acid micelle. Splenocytes (2 × 10 6 cells) were collected from the immunized mice on day 7 after immunization and were cultured with ( a ) or without ( b ) OVA CTL epitope peptide (SIINFEKL) for 40 h. ( a , b ) Typical micrographs of IFN-γ-producing spots measured by ELIspot. The number of IFN-γ-producing spots is shown ( c ).

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Mouse Assay, Cell Culture, CTL Assay, Enzyme-linked Immunospot

    Design of pH-responsive micelles composed of DLPC and deoxycholic acid for antigen delivery into cytosol of dendritic cells and induction of antigen presentation via MHC class I molecules (cross-presentation), which leads to antigen-specific cellular immunity. An atomic force microscope (AFM) image for DLPC/deoyxcholic acid micelles is also shown.

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: Design of pH-responsive micelles composed of DLPC and deoxycholic acid for antigen delivery into cytosol of dendritic cells and induction of antigen presentation via MHC class I molecules (cross-presentation), which leads to antigen-specific cellular immunity. An atomic force microscope (AFM) image for DLPC/deoyxcholic acid micelles is also shown.

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Microscopy