Structured Review

Nacalai deoxycholate
Chain form confers attenuated resistance to <t>deoxycholate.</t> GFP-expressing S . Tm ssaV or ssaV amiA amiC mutants were grown in LB or LB plus 0.5 M NaCl, and subsequently were mixed with 1% deoxycholate (DOC), followed by incubation and treatment with PI. The resulting S . Tm cells were placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar, 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. ns, not significant; * P
Deoxycholate, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxycholate/product/Nacalai
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deoxycholate - by Bioz Stars, 2021-03
86/100 stars

Images

1) Product Images from "Tat-exported peptidoglycan amidase-dependent cell division contributes to Salmonella Typhimurium fitness in the inflamed gut"

Article Title: Tat-exported peptidoglycan amidase-dependent cell division contributes to Salmonella Typhimurium fitness in the inflamed gut

Journal: PLoS Pathogens

doi: 10.1371/journal.ppat.1007391

Chain form confers attenuated resistance to deoxycholate. GFP-expressing S . Tm ssaV or ssaV amiA amiC mutants were grown in LB or LB plus 0.5 M NaCl, and subsequently were mixed with 1% deoxycholate (DOC), followed by incubation and treatment with PI. The resulting S . Tm cells were placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar, 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. ns, not significant; * P
Figure Legend Snippet: Chain form confers attenuated resistance to deoxycholate. GFP-expressing S . Tm ssaV or ssaV amiA amiC mutants were grown in LB or LB plus 0.5 M NaCl, and subsequently were mixed with 1% deoxycholate (DOC), followed by incubation and treatment with PI. The resulting S . Tm cells were placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar, 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. ns, not significant; * P

Techniques Used: Expressing, Incubation, Fluorescence, Microscopy, Staining

2) Product Images from "Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract"

Article Title: Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract

Journal: Infection and Immunity

doi: 10.1128/IAI.00892-19

Chain of C. rodentium by cell division defect confers attenuated resistance to deoxycholate. GFP-expressing C. rodentium wild-type strain (wt, T307) or tatC mutant ( tatC , T329) grown in LB medium was incubated with 0.5% deoxycholate (DOC), followed by treatment with propidium iodide (PI) solution to evaluate the DOC-mediated killing. The mixture was placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar = 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. The horizontal bars indicate the median values. ***, P
Figure Legend Snippet: Chain of C. rodentium by cell division defect confers attenuated resistance to deoxycholate. GFP-expressing C. rodentium wild-type strain (wt, T307) or tatC mutant ( tatC , T329) grown in LB medium was incubated with 0.5% deoxycholate (DOC), followed by treatment with propidium iodide (PI) solution to evaluate the DOC-mediated killing. The mixture was placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar = 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. The horizontal bars indicate the median values. ***, P

Techniques Used: Expressing, Mutagenesis, Incubation, Fluorescence, Microscopy, Staining

Related Articles

Western Blot:

Article Title: Changes of drug pharmacokinetics mediated by downregulation of kidney organic cation transporters Mate1 and Oct2 in a rat model of hyperuricemia
Article Snippet: Total clearance (CLtot ) was estimated as dose (D) over AUCinf (D/AUCinf ), and renal clearance (CLR ) was estimated as Xurine,0-4 /AUC0-4 , where Xurine,0–4 represents the cumulative amount of drug recovered in the urine from 0 to 4 h. The apparent tissue-to-plasma concentration ratio (Kp, kidney ) was obtained as the ratio of kidney concentration divided by plasma concentration at 4 h after administration. .. Western blot analysis Kidney tissue was homogenized in RIPA buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1.0% (v/v) nonidet P-40, 0.1% sodium dodecyl sulfate (SDS) and 0.5% (w/v) sodium deoxycholate) containing 1% protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan) using Ultra-Turrax homogenizer (model: T-25, IKA, Staufen, Germany). ..

Article Title: Methylseleninic acid induces NAD(P)H:quinone oxidoreductase-1 expression through activation of NF-E2-related factor 2 in Chang liver cells
Article Snippet: The DNA–protein complexes formed were resolved by 6% non-denaturating polyacrylamide gel electrophoresis carried out at 170 V for 2 h. After a vacuum-drying, the gel was exposed to X-ray film and kept at -70°C for autoradiography. .. Immunoprecipitation and Western blot analysis After treatment with MSeA, Chang liver cells (3 × 106 ) were lysed with modified RIPA buffer containing 10 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40, 1% deoxycholate, 50 mM sodium fluoride, 50 mM sodium orthovanadate, 1 mM PMSF, and proteinase inhibitor cocktail (Nacarai Tesque, Kyoto, Japan). .. The lysates were homogenized in an ultrasonicator for 10 sec twice and incubated on ice for 30 min.

Article Title: Sirtuin 7 Deficiency Ameliorates Cisplatin-induced Acute Kidney Injury Through Regulation of the Inflammatory Response
Article Snippet: Relative gene expression was obtained using the ΔΔCt method (Ct sample − Ct calibrator ). .. Western Blotting Total lysates of cells and kidney tissues were obtained by lysis in RIPA Buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 0.1% sodium dodecyl sulfate (SDS), 1% NP-40, 5 mM EDTA, 0.5% sodium deoxycholate, 20 mg/mL Na3VO4, 10 mM NaF, and 1 mM PMSF) with a protease inhibitor cocktail (Nacalai Tesque). .. Nuclear fractions of cells and kidney tissues were prepared using a LysoPure™ Nuclear and Cytoplasmic Extractor Kit (Wako) according to the manufacturer’s protocol.

Protease Inhibitor:

Article Title: Changes of drug pharmacokinetics mediated by downregulation of kidney organic cation transporters Mate1 and Oct2 in a rat model of hyperuricemia
Article Snippet: Total clearance (CLtot ) was estimated as dose (D) over AUCinf (D/AUCinf ), and renal clearance (CLR ) was estimated as Xurine,0-4 /AUC0-4 , where Xurine,0–4 represents the cumulative amount of drug recovered in the urine from 0 to 4 h. The apparent tissue-to-plasma concentration ratio (Kp, kidney ) was obtained as the ratio of kidney concentration divided by plasma concentration at 4 h after administration. .. Western blot analysis Kidney tissue was homogenized in RIPA buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1.0% (v/v) nonidet P-40, 0.1% sodium dodecyl sulfate (SDS) and 0.5% (w/v) sodium deoxycholate) containing 1% protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan) using Ultra-Turrax homogenizer (model: T-25, IKA, Staufen, Germany). ..

Article Title: Sirtuin 7 Deficiency Ameliorates Cisplatin-induced Acute Kidney Injury Through Regulation of the Inflammatory Response
Article Snippet: Relative gene expression was obtained using the ΔΔCt method (Ct sample − Ct calibrator ). .. Western Blotting Total lysates of cells and kidney tissues were obtained by lysis in RIPA Buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 0.1% sodium dodecyl sulfate (SDS), 1% NP-40, 5 mM EDTA, 0.5% sodium deoxycholate, 20 mg/mL Na3VO4, 10 mM NaF, and 1 mM PMSF) with a protease inhibitor cocktail (Nacalai Tesque). .. Nuclear fractions of cells and kidney tissues were prepared using a LysoPure™ Nuclear and Cytoplasmic Extractor Kit (Wako) according to the manufacturer’s protocol.

Article Title: Nucleolar Stress Induces Ubiquitination-independent Proteasomal Degradation of PICT1 Protein *
Article Snippet: After a 2-day incubation, nuclei were isolated as described on the Lamond Lab web site and in Refs. , . .. The nuclei (3 × 107 ) were then resuspended in 0.2 ml of high salt RIPA buffer (50 m m HEPES-NaOH, 500 m m NaCl, 1% (w/v) Nonidet P-40, 0.5% (w/v) sodium deoxycholate, protease inhibitor mixture (Nacalai Tesque, Kyoto, Japan), 50 units of Benzonase (EMD Chemicals, Inc., Billerica, CA), pH 7.4) and incubated for 30 min at 4 °C. .. The suspension was sonicated to disrupt nuclei, and insoluble materials were precipitated by centrifugation (12,000 × g , 15 min, 4 °C).

Lysis:

Article Title: GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance
Article Snippet: The supernatant was collected and rotated gently for 90 min at 4 °C after adding 20 μl anti-Flag antibody beads (Sigma). .. In the experiment to detect the endogenous interaction between GRK6 and GIT1 in BMDM, the cells were lysed in lysis buffer 2 (50 mM Tris-Cl (pH 7.5), 300 mM NaCl, 0.1% SDS, 1% Triton X-100, 0.5% Sodium deoxycholate, 1 mM Na3 VO4 and 1 mM NaF) containing protease inhibitors (Nacalai). ..

Article Title: Sirtuin 7 Deficiency Ameliorates Cisplatin-induced Acute Kidney Injury Through Regulation of the Inflammatory Response
Article Snippet: Relative gene expression was obtained using the ΔΔCt method (Ct sample − Ct calibrator ). .. Western Blotting Total lysates of cells and kidney tissues were obtained by lysis in RIPA Buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 0.1% sodium dodecyl sulfate (SDS), 1% NP-40, 5 mM EDTA, 0.5% sodium deoxycholate, 20 mg/mL Na3VO4, 10 mM NaF, and 1 mM PMSF) with a protease inhibitor cocktail (Nacalai Tesque). .. Nuclear fractions of cells and kidney tissues were prepared using a LysoPure™ Nuclear and Cytoplasmic Extractor Kit (Wako) according to the manufacturer’s protocol.

Modification:

Article Title: Antioxidant Properties of a Traditional Vine Tea, Ampelopsis grossedentata
Article Snippet: .. The cells were harvested with modified RIPA buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1% Nonidet P-40, 0.25% Na-deoxycholate, 1 mM sodium fluoride, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride) plus proteinase inhibitor cocktail (Nacalai Tesque, Inc., Kyoto, Japan). .. Equal amounts of lysate protein were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane electrophoretically (GE Healthcare UK Ltd., Amersham, England).

Article Title: Methylseleninic acid induces NAD(P)H:quinone oxidoreductase-1 expression through activation of NF-E2-related factor 2 in Chang liver cells
Article Snippet: The DNA–protein complexes formed were resolved by 6% non-denaturating polyacrylamide gel electrophoresis carried out at 170 V for 2 h. After a vacuum-drying, the gel was exposed to X-ray film and kept at -70°C for autoradiography. .. Immunoprecipitation and Western blot analysis After treatment with MSeA, Chang liver cells (3 × 106 ) were lysed with modified RIPA buffer containing 10 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40, 1% deoxycholate, 50 mM sodium fluoride, 50 mM sodium orthovanadate, 1 mM PMSF, and proteinase inhibitor cocktail (Nacarai Tesque, Kyoto, Japan). .. The lysates were homogenized in an ultrasonicator for 10 sec twice and incubated on ice for 30 min.

Immunoprecipitation:

Article Title: Methylseleninic acid induces NAD(P)H:quinone oxidoreductase-1 expression through activation of NF-E2-related factor 2 in Chang liver cells
Article Snippet: The DNA–protein complexes formed were resolved by 6% non-denaturating polyacrylamide gel electrophoresis carried out at 170 V for 2 h. After a vacuum-drying, the gel was exposed to X-ray film and kept at -70°C for autoradiography. .. Immunoprecipitation and Western blot analysis After treatment with MSeA, Chang liver cells (3 × 106 ) were lysed with modified RIPA buffer containing 10 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.1% Nonidet P-40, 1% deoxycholate, 50 mM sodium fluoride, 50 mM sodium orthovanadate, 1 mM PMSF, and proteinase inhibitor cocktail (Nacarai Tesque, Kyoto, Japan). .. The lysates were homogenized in an ultrasonicator for 10 sec twice and incubated on ice for 30 min.

Incubation:

Article Title: Tat-exported peptidoglycan amidase-dependent cell division contributes to Salmonella Typhimurium fitness in the inflamed gut
Article Snippet: .. Tm strains grown to the logarithmic growth phase were diluted to 1×106 CFU per ml with different concentrations of magainin 2 (LKT Laboratories, Inc.) or deoxycholate (Nacalai tesque) in sterile LB broth, and incubated for 15 h at 37°C. .. After incubation, the A 595 values were determined using a microplate reader (Bio-Rad).

Article Title: Nucleolar Stress Induces Ubiquitination-independent Proteasomal Degradation of PICT1 Protein *
Article Snippet: After a 2-day incubation, nuclei were isolated as described on the Lamond Lab web site and in Refs. , . .. The nuclei (3 × 107 ) were then resuspended in 0.2 ml of high salt RIPA buffer (50 m m HEPES-NaOH, 500 m m NaCl, 1% (w/v) Nonidet P-40, 0.5% (w/v) sodium deoxycholate, protease inhibitor mixture (Nacalai Tesque, Kyoto, Japan), 50 units of Benzonase (EMD Chemicals, Inc., Billerica, CA), pH 7.4) and incubated for 30 min at 4 °C. .. The suspension was sonicated to disrupt nuclei, and insoluble materials were precipitated by centrifugation (12,000 × g , 15 min, 4 °C).

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    Nacalai deoxycholate
    Chain form confers attenuated resistance to <t>deoxycholate.</t> GFP-expressing S . Tm ssaV or ssaV amiA amiC mutants were grown in LB or LB plus 0.5 M NaCl, and subsequently were mixed with 1% deoxycholate (DOC), followed by incubation and treatment with PI. The resulting S . Tm cells were placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar, 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. ns, not significant; * P
    Deoxycholate, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholate/product/Nacalai
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxycholate - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Nacalai deoxycholic acid sodium salt monohydrate
    CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or <t>DLPC/deoxycholic</t> acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.
    Deoxycholic Acid Sodium Salt Monohydrate, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholic acid sodium salt monohydrate/product/Nacalai
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxycholic acid sodium salt monohydrate - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Chain form confers attenuated resistance to deoxycholate. GFP-expressing S . Tm ssaV or ssaV amiA amiC mutants were grown in LB or LB plus 0.5 M NaCl, and subsequently were mixed with 1% deoxycholate (DOC), followed by incubation and treatment with PI. The resulting S . Tm cells were placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar, 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. ns, not significant; * P

    Journal: PLoS Pathogens

    Article Title: Tat-exported peptidoglycan amidase-dependent cell division contributes to Salmonella Typhimurium fitness in the inflamed gut

    doi: 10.1371/journal.ppat.1007391

    Figure Lengend Snippet: Chain form confers attenuated resistance to deoxycholate. GFP-expressing S . Tm ssaV or ssaV amiA amiC mutants were grown in LB or LB plus 0.5 M NaCl, and subsequently were mixed with 1% deoxycholate (DOC), followed by incubation and treatment with PI. The resulting S . Tm cells were placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar, 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. ns, not significant; * P

    Article Snippet: Tm strains grown to the logarithmic growth phase were diluted to 1×106 CFU per ml with different concentrations of magainin 2 (LKT Laboratories, Inc.) or deoxycholate (Nacalai tesque) in sterile LB broth, and incubated for 15 h at 37°C.

    Techniques: Expressing, Incubation, Fluorescence, Microscopy, Staining

    Chain of C. rodentium by cell division defect confers attenuated resistance to deoxycholate. GFP-expressing C. rodentium wild-type strain (wt, T307) or tatC mutant ( tatC , T329) grown in LB medium was incubated with 0.5% deoxycholate (DOC), followed by treatment with propidium iodide (PI) solution to evaluate the DOC-mediated killing. The mixture was placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar = 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. The horizontal bars indicate the median values. ***, P

    Journal: Infection and Immunity

    Article Title: Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract

    doi: 10.1128/IAI.00892-19

    Figure Lengend Snippet: Chain of C. rodentium by cell division defect confers attenuated resistance to deoxycholate. GFP-expressing C. rodentium wild-type strain (wt, T307) or tatC mutant ( tatC , T329) grown in LB medium was incubated with 0.5% deoxycholate (DOC), followed by treatment with propidium iodide (PI) solution to evaluate the DOC-mediated killing. The mixture was placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar = 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. The horizontal bars indicate the median values. ***, P

    Article Snippet: Briefly, C. rodentium strains were diluted to 1 × 106 CFU per ml with different concentrations of maga inin-2 (LKT Laboratories, Inc.) or deoxycholate (Nacalai Tesque) in sterile LB broth and incubated at 37°C.

    Techniques: Expressing, Mutagenesis, Incubation, Fluorescence, Microscopy, Staining

    CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or DLPC/deoxycholic acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: CLSM images of DC2.4 cells treated with FITC-OVA in the absence ( a ) or the presence of DLPC liposomes ( b ) or DLPC/deoxycholic acid micelles ( c ) for 5 h. Cells were also stained with LysoTracker Red. Scale bar represents 10 μm.

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Confocal Laser Scanning Microscopy, Staining

    Confocal laser scanning microscopic (CLSM) images of DC2.4 cells treated with DLPC liposomes ( a ), EYPC/deoxycholic acid micelles ( b ) and DLPC/deoxycholic acid micelles ( c ) for 5 h. Fluorescence of NBD-PE and Rh-PE upon excitation at 488 nm was observed using a CLSM. Scale bar represents 10 μm.

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: Confocal laser scanning microscopic (CLSM) images of DC2.4 cells treated with DLPC liposomes ( a ), EYPC/deoxycholic acid micelles ( b ) and DLPC/deoxycholic acid micelles ( c ) for 5 h. Fluorescence of NBD-PE and Rh-PE upon excitation at 488 nm was observed using a CLSM. Scale bar represents 10 μm.

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Confocal Laser Scanning Microscopy, Fluorescence

    pH-responsive properties of DLPC/deoxycholic acid micelles. ( a ) Time courses of pyranine fluorescence for pyranine-loaded EYPC liposomes after addition of DLPC/deoxycholic acid micelles at pH 7.4 (red) or 5.0 (blue). Triton-X100 was added to dissociate the liposomal membrane completely. ( b ) Comparison of membrane disruptive activity for pyranine-loaded EYPC liposomes by addition of DOPE/CHEMS (green), DOPE/oleic acid (red) and DLPC/deoxycholic acid micelles (blue) at various pH. ( c ) Lipid mixing assay using EYPC liposomes containing Rh-PE/NBD-PE and various vehicles. Ratios of fluorescence intensity at 530 nm and 580 nm, which indicate a decrease of FRET by lipid mixing, are shown as a function of pH. Methanol was used to achieve complete lipid mixing.

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: pH-responsive properties of DLPC/deoxycholic acid micelles. ( a ) Time courses of pyranine fluorescence for pyranine-loaded EYPC liposomes after addition of DLPC/deoxycholic acid micelles at pH 7.4 (red) or 5.0 (blue). Triton-X100 was added to dissociate the liposomal membrane completely. ( b ) Comparison of membrane disruptive activity for pyranine-loaded EYPC liposomes by addition of DOPE/CHEMS (green), DOPE/oleic acid (red) and DLPC/deoxycholic acid micelles (blue) at various pH. ( c ) Lipid mixing assay using EYPC liposomes containing Rh-PE/NBD-PE and various vehicles. Ratios of fluorescence intensity at 530 nm and 580 nm, which indicate a decrease of FRET by lipid mixing, are shown as a function of pH. Methanol was used to achieve complete lipid mixing.

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Fluorescence, Activity Assay

    Interaction of DLPC/deoxycholic acid micelles with DC2.4 cells. ( a ) Effects of temperature for cellular association of micelles. DC2.4 cells were treated with Rh-PE-labeled micelles for 5 h at various temperatures. Cellular fluorescence was measured using a flow cytometer. ( b – d ) Effect of inhibitors for cellular association of micelles. Cellular association of Rh-PE-labeled micelles in the presence of various amounts of indicating inhibitors was evaluated. * p

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: Interaction of DLPC/deoxycholic acid micelles with DC2.4 cells. ( a ) Effects of temperature for cellular association of micelles. DC2.4 cells were treated with Rh-PE-labeled micelles for 5 h at various temperatures. Cellular fluorescence was measured using a flow cytometer. ( b – d ) Effect of inhibitors for cellular association of micelles. Cellular association of Rh-PE-labeled micelles in the presence of various amounts of indicating inhibitors was evaluated. * p

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Labeling, Fluorescence, Flow Cytometry, Cytometry

    Induction of cellular immunity by pH-responsive micelles. C57BL/6 mice were immunized intradermally with 16 μg of OVA, OVA + CpG-ODN, or OVA + DLPC/deoxycholic acid micelle or OVA + CpG-ODN + DLPC/deoxycholic acid micelle. Splenocytes (2 × 10 6 cells) were collected from the immunized mice on day 7 after immunization and were cultured with ( a ) or without ( b ) OVA CTL epitope peptide (SIINFEKL) for 40 h. ( a , b ) Typical micrographs of IFN-γ-producing spots measured by ELIspot. The number of IFN-γ-producing spots is shown ( c ).

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: Induction of cellular immunity by pH-responsive micelles. C57BL/6 mice were immunized intradermally with 16 μg of OVA, OVA + CpG-ODN, or OVA + DLPC/deoxycholic acid micelle or OVA + CpG-ODN + DLPC/deoxycholic acid micelle. Splenocytes (2 × 10 6 cells) were collected from the immunized mice on day 7 after immunization and were cultured with ( a ) or without ( b ) OVA CTL epitope peptide (SIINFEKL) for 40 h. ( a , b ) Typical micrographs of IFN-γ-producing spots measured by ELIspot. The number of IFN-γ-producing spots is shown ( c ).

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Mouse Assay, Cell Culture, CTL Assay, Enzyme-linked Immunospot

    Design of pH-responsive micelles composed of DLPC and deoxycholic acid for antigen delivery into cytosol of dendritic cells and induction of antigen presentation via MHC class I molecules (cross-presentation), which leads to antigen-specific cellular immunity. An atomic force microscope (AFM) image for DLPC/deoyxcholic acid micelles is also shown.

    Journal: Vaccines

    Article Title: pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity

    doi: 10.3390/vaccines5040041

    Figure Lengend Snippet: Design of pH-responsive micelles composed of DLPC and deoxycholic acid for antigen delivery into cytosol of dendritic cells and induction of antigen presentation via MHC class I molecules (cross-presentation), which leads to antigen-specific cellular immunity. An atomic force microscope (AFM) image for DLPC/deoyxcholic acid micelles is also shown.

    Article Snippet: Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Microscopy