deoxycholate (Nacalai)
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Deoxycholate, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "Tat-exported peptidoglycan amidase-dependent cell division contributes to Salmonella Typhimurium fitness in the inflamed gut"
Article Title: Tat-exported peptidoglycan amidase-dependent cell division contributes to Salmonella Typhimurium fitness in the inflamed gut
Journal: PLoS Pathogens
doi: 10.1371/journal.ppat.1007391

Figure Legend Snippet: Chain form confers attenuated resistance to deoxycholate. GFP-expressing S . Tm ssaV or ssaV amiA amiC mutants were grown in LB or LB plus 0.5 M NaCl, and subsequently were mixed with 1% deoxycholate (DOC), followed by incubation and treatment with PI. The resulting S . Tm cells were placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar, 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. ns, not significant; * P
Techniques Used: Expressing, Incubation, Fluorescence, Microscopy, Staining
2) Product Images from "Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract"
Article Title: Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract
Journal: Infection and Immunity
doi: 10.1128/IAI.00892-19

Figure Legend Snippet: Chain of C. rodentium by cell division defect confers attenuated resistance to deoxycholate. GFP-expressing C. rodentium wild-type strain (wt, T307) or tatC mutant ( tatC , T329) grown in LB medium was incubated with 0.5% deoxycholate (DOC), followed by treatment with propidium iodide (PI) solution to evaluate the DOC-mediated killing. The mixture was placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar = 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. The horizontal bars indicate the median values. ***, P
Techniques Used: Expressing, Mutagenesis, Incubation, Fluorescence, Microscopy, Staining
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