Structured Review

Amresco deoxycholate
Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) <t>deoxycholate,</t> (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ
Deoxycholate, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts"

Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

Journal: Biochemistry

doi: 10.1021/bi100335u

Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) deoxycholate, (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ
Figure Legend Snippet: Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) deoxycholate, (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ

Techniques Used:

(A) Overlay of four 2D 1 H- 15 N TROSY spectra of [ 2 H, 15 N, 13 C]-labeled SipD 39–343 with increasing amounts of deoxycholate. Some of the assignments are indicated as well as the noise peaks (*). (B) Expanded regions of the 2D 1 H- 15 N TROSY spectra for
Figure Legend Snippet: (A) Overlay of four 2D 1 H- 15 N TROSY spectra of [ 2 H, 15 N, 13 C]-labeled SipD 39–343 with increasing amounts of deoxycholate. Some of the assignments are indicated as well as the noise peaks (*). (B) Expanded regions of the 2D 1 H- 15 N TROSY spectra for

Techniques Used: Labeling

Ribbon representation of the crystal structure of SipD 39–343 (to be reported elsewhere). The side chains of residues that showed significant chemical shift perturbation (Δδ HN > 0.03) with deoxycholate were colored red (legend:
Figure Legend Snippet: Ribbon representation of the crystal structure of SipD 39–343 (to be reported elsewhere). The side chains of residues that showed significant chemical shift perturbation (Δδ HN > 0.03) with deoxycholate were colored red (legend:

Techniques Used:

Related Articles

Incubation:

Article Title: Wnt Signaling Inhibition Deprives Small Intestinal Stem Cells of Clonogenic Capacity
Article Snippet: .. The LacZ substrate [5 mM K3 FE(CN)6 , 5 mM K4 FE(CN)6 × 3H2 0, 2 mM MgCl2 , 0.02% NP40, 0.1% sodium deoxycholate, and 1 mg/mL X‐gal (Amresco) in PBS] was added and tissues were incubated overnight at RT protected from the light. ..

Radio Immunoprecipitation:

Article Title: Differential Activation of Cellular DNA Damage Responses by Replication-Defective and Replication-Competent Adenovirus Mutants
Article Snippet: Cells from each 35-mm dish were harvested, washed with ice-cold PBS, and pelleted by centrifugation. .. Cell pellets were lysed in 200 μl of radioimmunoprecipitation assay lysis buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 0.1% sodium dodecyl sulfate [SDS], 1.0% Igepal CA-630, 0.5% deoxycholic acid) supplemented with protease inhibitors, aprotinin, and leupeptin at 5 μg/ml (Amresco) and a phosphatase inhibitor cocktail in accordance with the manufacturer's instructions (Sigma-Aldrich). .. Cell lysates were sonicated, and total protein levels in samples were measured by Bradford assay using Coomassie Plus protein reagent (Pierce), according to the manufacturer's instructions.

Lysis:

Article Title: Differential Activation of Cellular DNA Damage Responses by Replication-Defective and Replication-Competent Adenovirus Mutants
Article Snippet: Cells from each 35-mm dish were harvested, washed with ice-cold PBS, and pelleted by centrifugation. .. Cell pellets were lysed in 200 μl of radioimmunoprecipitation assay lysis buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 0.1% sodium dodecyl sulfate [SDS], 1.0% Igepal CA-630, 0.5% deoxycholic acid) supplemented with protease inhibitors, aprotinin, and leupeptin at 5 μg/ml (Amresco) and a phosphatase inhibitor cocktail in accordance with the manufacturer's instructions (Sigma-Aldrich). .. Cell lysates were sonicated, and total protein levels in samples were measured by Bradford assay using Coomassie Plus protein reagent (Pierce), according to the manufacturer's instructions.

other:

Article Title: Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein
Article Snippet: Washing the IB pellet with 0.1% deoxycholic acid helped in the removal of contaminating membrane proteins.

Sonication:

Article Title: Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein
Article Snippet: Sonicated cells were centrifuged at 12,000 rpm (SA-300 rotor, Sorvall evolution RC) for 20 min at 4°C and the pellet containing recombinant asparaginase as IBs was separated. .. These pellets were resuspended in 20 ml buffer I containing 0.1% (w/v) deoxycholic acid, sonicated and centrifuged as described earlier (Patra et al., ). ..

Protease Inhibitor:

Article Title: A Synthetic Manassantin A Derivative Inhibits Hypoxia-Inducible Factor 1 and Tumor Growth
Article Snippet: Nuclear extracts were prepared using the Nuclear and Cytoplasmic Extraction Reagents (CWBio Co., Beijing, China). .. Cells were lysed using RIPA buffer containing 50 mM Tris-HCl, pH7.4, 150 mM NaCl, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, and protease inhibitor cocktails (Amresco, USA). .. Equal amounts of proteins were resolved by SDS-PAGE gels and transferred to PVDF membranes (Millipore, USA).

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    Amresco deoxycholate
    Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) <t>deoxycholate,</t> (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ
    Deoxycholate, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholate/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxycholate - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Amresco m sodium deoxycholate
    Crystal structure of the <t>SipD-deoxycholate</t> complex. Deoxycholate (shown as a stick model) binds at the interface of molecules A and B of the asymmetric unit. SipD is colored as follows: the coiled coil (helix α4 and α8), gray; the N-terminal
    M Sodium Deoxycholate, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m sodium deoxycholate/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m sodium deoxycholate - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Amresco sodium deoxycholate solution
    Crystal structure of the <t>SipD-deoxycholate</t> complex. Deoxycholate (shown as a stick model) binds at the interface of molecules A and B of the asymmetric unit. SipD is colored as follows: the coiled coil (helix α4 and α8), gray; the N-terminal
    Sodium Deoxycholate Solution, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium deoxycholate solution/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium deoxycholate solution - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) deoxycholate, (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ

    Journal: Biochemistry

    Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

    doi: 10.1021/bi100335u

    Figure Lengend Snippet: Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) deoxycholate, (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ

    Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

    Techniques:

    (A) Overlay of four 2D 1 H- 15 N TROSY spectra of [ 2 H, 15 N, 13 C]-labeled SipD 39–343 with increasing amounts of deoxycholate. Some of the assignments are indicated as well as the noise peaks (*). (B) Expanded regions of the 2D 1 H- 15 N TROSY spectra for

    Journal: Biochemistry

    Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

    doi: 10.1021/bi100335u

    Figure Lengend Snippet: (A) Overlay of four 2D 1 H- 15 N TROSY spectra of [ 2 H, 15 N, 13 C]-labeled SipD 39–343 with increasing amounts of deoxycholate. Some of the assignments are indicated as well as the noise peaks (*). (B) Expanded regions of the 2D 1 H- 15 N TROSY spectra for

    Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

    Techniques: Labeling

    Ribbon representation of the crystal structure of SipD 39–343 (to be reported elsewhere). The side chains of residues that showed significant chemical shift perturbation (Δδ HN > 0.03) with deoxycholate were colored red (legend:

    Journal: Biochemistry

    Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

    doi: 10.1021/bi100335u

    Figure Lengend Snippet: Ribbon representation of the crystal structure of SipD 39–343 (to be reported elsewhere). The side chains of residues that showed significant chemical shift perturbation (Δδ HN > 0.03) with deoxycholate were colored red (legend:

    Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

    Techniques:

    Crystal structure of the SipD-deoxycholate complex. Deoxycholate (shown as a stick model) binds at the interface of molecules A and B of the asymmetric unit. SipD is colored as follows: the coiled coil (helix α4 and α8), gray; the N-terminal

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate

    doi: 10.1002/pro.537

    Figure Lengend Snippet: Crystal structure of the SipD-deoxycholate complex. Deoxycholate (shown as a stick model) binds at the interface of molecules A and B of the asymmetric unit. SipD is colored as follows: the coiled coil (helix α4 and α8), gray; the N-terminal

    Article Snippet: A SipD C244S-deoxycholate solution was prepared by mixing 1 μL 400 m M sodium deoxycholate (Amresco) with 100 μL of 0.3 m M SipD C244S.

    Techniques:

    Fo-Fc omit maps contoured at 3σ of (A) deoxycholate and (B) chenodeoxycholate in complex with SipD shown with residues within 5 Å of bile salts (color scheme: molecule A, yellow; molecule B, cyan; and another molecule A related by a crystallographic

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate

    doi: 10.1002/pro.537

    Figure Lengend Snippet: Fo-Fc omit maps contoured at 3σ of (A) deoxycholate and (B) chenodeoxycholate in complex with SipD shown with residues within 5 Å of bile salts (color scheme: molecule A, yellow; molecule B, cyan; and another molecule A related by a crystallographic

    Article Snippet: A SipD C244S-deoxycholate solution was prepared by mixing 1 μL 400 m M sodium deoxycholate (Amresco) with 100 μL of 0.3 m M SipD C244S.

    Techniques: