denhardt s  (Millipore)

 
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    Name:
    Denhardt s Solution
    Description:
    Denhardt s Solution is a blocking agent routinely used to reduce non specific binding of detection reagents to the Northern and Southern Blot membranes This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre hybridization and hybridization buffers
    Catalog Number:
    D9905
    Price:
    None
    Applications:
    Suitable as a blocking Buffer for Southern and Northern blot membranes.
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    Structured Review

    Millipore denhardt s
    Denhardt s Solution is a blocking agent routinely used to reduce non specific binding of detection reagents to the Northern and Southern Blot membranes This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre hybridization and hybridization buffers
    https://www.bioz.com/result/denhardt s/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    denhardt s - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Hybridization:

    Article Title: Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays
    Article Snippet: Tubes were incubated overnight at −20°C, and nucleic acids were collected by centrifugation at 14,000 × g for 30 min. .. The resulting (dark-brown) pellet was washed in 70% ethanol, dried under a vacuum, resuspended in hybridization buffer (4× SSPE [1× SSPE is 0.18 M NaCl, 10 mM NaH2 PO4 , and 1 mM EDTA {pH 7.7}]–2.5× Denhardt's solution [50× Denhardt's solution is 10 g of Ficoll 400 {Sigma, St. Louis, Mo.} liter−1 , 10 g of polyvinylpyrrolidone (Sigma) liter−1 , and 10 g of ultrapure bovine serum albumin {Ambion} liter−1 ], with or without 30% formamide), and stored at −20°C until use. .. An alignment of Geobacter and Desulfovibrio 16S rRNA sequences was generated from GenBank and Ribosomal Database Project (RDP) ( ) entries and used to develop a set of capture and detector probes for this study (Table ).

    Article Title: Saci-1, -2, and -3 and Perere, Four Novel Retrotransposons with High Transcriptional Activities from the Human Parasite Schistosoma mansoni
    Article Snippet: Radiolabeled probes for each retrotransposon were generated from 25 ng of fragments of approximately 700 bp labeled with [32 P]dCTP by using Rediprime kits (Amersham Biosciences). .. Overnight hybridization was performed in 6× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate)-0.5% sodium dodecyl sulfate (SDS)-5× Denhardt's solution-25 μg of salmon testis DNA (Sigma)/ml at 68°C. ..

    Article Title: Leptin Induces Phosphorylation of Neuronal Nitric Oxide Synthase in Defined Hypothalamic Neurons
    Article Snippet: The nNOS riboprobe was generated by in vitro transcription with 35 S-uridine 5-triphosphate. .. The 35 S-labeled probe was diluted (106 dpm/ml) in hybridization solution containing 50% formamide, 10% dextran sulfate, and 1× Denhardt’s solution (Sigma). .. The hybridization solution (120 μl) was applied to each slide and they were incubated overnight at 56 C. Sections were then treated with 0.002% ribonuclease A solution and submitted to stringency washes in decreasing concentrations of sodium chloride/sodium citrate buffer.

    Article Title: Intron-Specific Neuropeptide Probes
    Article Snippet: For nucleic acid mix: salmon sperm DNA (available from Sigma Chemicals or Invitrogen), Yeast total RNA type XI (ribonucleic acid – Sigma Chemicals), Yeast tRNA (Invitrogen). .. Hybridization buffers: Tris–Cl, pH 7.4, EDTA pH 8.0, NaCl, Formamide, Dextran sulfate, Denhardt’s solution, DTT, sodium dodecyl sulfate (SDS), sodium thiosulfate (NTS – Sigma Chemicals), nuclease-free water. .. SSC buffer: 0.3 M sodium citrate, 3 M NaCl, pH 7.0.

    Article Title: Neuregulin1 Fine-tunes Pre-, Post-, and Peri-Synaptic Neuromuscular Junction Development
    Article Snippet: Afterward, the sections were incubated with 2 μg/ml probe in hybridization buffer at 60°C overnight. .. Hybridization buffer contained 50% formamide, 0.3 M NaCl, 0.5 mg/ml yeast tRNA, 1x Denhardt’s solution (Sigma), 20 mM Tris, pH 7.4, 10% Dextran Sulphate, 10 mM NaPO4 and 5 mM EDTA. .. After hybridization, the sections were sequentially washed in 5x SSC, 50% formamide in 1x SSC, 2x SSC, and 0.2x SSC for 10, 30, 20, and 20 min at 65°C sequentially.

    Article Title: An optimized microarray platform for assaying genomic variation in Plasmodium falciparum field populations
    Article Snippet: .. Labeled product was resuspended in water, and 10 μg of test and reference samples combined (6 μg for 5K SNP chip samples), dried down, and resuspended in hybridization buffer (Roche NimbleGen, Inc., Madison, WI, USA); hybridizations for patient blood samples included 1× Denhardt's solution (Sigma Aldrich) in the hybridization buffer. .. The combined sample was denatured at 95°C for 5 minutes and allowed to hybridize on the array for 24 h (16 h for 5K SNP chip samples) at 42°C in a NimbleGen hybridization system (Roche NimbleGen, Inc.).

    Article Title: Deletion of Interleukin-6 Signal Transducer gp130 in Small Sensory Neurons Attenuates Mechanonociception and Down-Regulates TRPA1 Expression
    Article Snippet: Cryostat sections (12 μm) were mounted on Super Frost Plus slides (Thermo Scientific), air-dried for 1–3 h at room temperature, fixed with 4% PFA for 30 min, and prehybridized with equilibration buffer containing the following: 12.6 m m Tris (Roth) at pH 7.5, 185 m m NaCl (Sigma), 10 m m NaH2 PO4 (Merck), 5 m m EDTA (Sigma), 50% deionized formamide (Sigma), 0.5× Denhardt's (Sigma) in DEPC-treated H2 O for 5–15 min at room temperature. .. Hybridization was performed overnight at 68°C in a humidified chamber. cRNA probes were diluted 1:50 in hybridization buffer containing 12.6 m m Tris (Roth), pH 7.5, 185 m m NaCl (Sigma), 10 m m NaH2 PO4 (Merck), 5 m m EDTA (Sigma), 50% deionized formamide (Sigma), 10% dextrane sulfate (Sigma), 1 mg/ml yeast RNA (Roche), and 0.5× Denhardt's (Sigma) in DEPC-treated H2 O. .. Sections were then washed two times for 30 min at 68°C in 1× SSC (Sigma), pH 7.0, 25% formamide, 0.1% Tween 20 (Sigma), twice for 30 min and once for 1 h at room temperature in 100 m m maleic acid (Sigma), 150 m m NaCl (Sigma), and 0.1% Tween 20 (Sigma), pH 7.5 (MABT).

    Article Title: Identification in GRMD dog muscle of critical miRNAs involved in pathophysiology and effects associated with MuStem cell transplantation
    Article Snippet: .. Then, the sections were permeabilized with proteinase K (20 μg/mL) for 10 min. For pre-hybridization, the tissue sections were covered for 1 h with hybridization buffer containing 50 % formamide, 4X SSC, 1X Denhardt’s solution, 500 μg/mL salmon sperm DNA (Sigma-Aldrich, Saint Quentin Fallavier, France), 10 % dextran sulfate and 1X Blocking Reagent (Roche, Basel, Switzerland). .. For hybridization, 50 nM of DIG-labelled probes diluted in hybridization buffer were applied per section and incubated in a sealed humidified chamber for 16 h at 55 °C.

    Labeling:

    Article Title: An optimized microarray platform for assaying genomic variation in Plasmodium falciparum field populations
    Article Snippet: .. Labeled product was resuspended in water, and 10 μg of test and reference samples combined (6 μg for 5K SNP chip samples), dried down, and resuspended in hybridization buffer (Roche NimbleGen, Inc., Madison, WI, USA); hybridizations for patient blood samples included 1× Denhardt's solution (Sigma Aldrich) in the hybridization buffer. .. The combined sample was denatured at 95°C for 5 minutes and allowed to hybridize on the array for 24 h (16 h for 5K SNP chip samples) at 42°C in a NimbleGen hybridization system (Roche NimbleGen, Inc.).

    Chromatin Immunoprecipitation:

    Article Title: An optimized microarray platform for assaying genomic variation in Plasmodium falciparum field populations
    Article Snippet: .. Labeled product was resuspended in water, and 10 μg of test and reference samples combined (6 μg for 5K SNP chip samples), dried down, and resuspended in hybridization buffer (Roche NimbleGen, Inc., Madison, WI, USA); hybridizations for patient blood samples included 1× Denhardt's solution (Sigma Aldrich) in the hybridization buffer. .. The combined sample was denatured at 95°C for 5 minutes and allowed to hybridize on the array for 24 h (16 h for 5K SNP chip samples) at 42°C in a NimbleGen hybridization system (Roche NimbleGen, Inc.).

    Blocking Assay:

    Article Title: Identification in GRMD dog muscle of critical miRNAs involved in pathophysiology and effects associated with MuStem cell transplantation
    Article Snippet: .. Then, the sections were permeabilized with proteinase K (20 μg/mL) for 10 min. For pre-hybridization, the tissue sections were covered for 1 h with hybridization buffer containing 50 % formamide, 4X SSC, 1X Denhardt’s solution, 500 μg/mL salmon sperm DNA (Sigma-Aldrich, Saint Quentin Fallavier, France), 10 % dextran sulfate and 1X Blocking Reagent (Roche, Basel, Switzerland). .. For hybridization, 50 nM of DIG-labelled probes diluted in hybridization buffer were applied per section and incubated in a sealed humidified chamber for 16 h at 55 °C.

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  • 93
    Millipore denhardt s solution
    Field sample analysis with the CNV-SNP array . (a) The manufacturer-recommended starting amount is 1,000 ng of DNA to produce at least 10 μg of labeled product. However, 250 ng of parasite DNA consistently produced sufficient labeled product when using 65% AT nonamers. Error bars indicate one standard deviation. (b) Hybridizations with field samples - straight from patient blood, or whole genome amplified - produced microarray data on par with standard lab clones, even when significant human DNA contamination was present. Microarray accuracy was determined through Illumina sequencing of lab-adapted parasites. Patient blood samples were hybridized with the addition of 1× <t>Denhardt's</t> solution while WGA samples were not.
    Denhardt S Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/denhardt s solution/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    denhardt s solution - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

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    Field sample analysis with the CNV-SNP array . (a) The manufacturer-recommended starting amount is 1,000 ng of DNA to produce at least 10 μg of labeled product. However, 250 ng of parasite DNA consistently produced sufficient labeled product when using 65% AT nonamers. Error bars indicate one standard deviation. (b) Hybridizations with field samples - straight from patient blood, or whole genome amplified - produced microarray data on par with standard lab clones, even when significant human DNA contamination was present. Microarray accuracy was determined through Illumina sequencing of lab-adapted parasites. Patient blood samples were hybridized with the addition of 1× Denhardt's solution while WGA samples were not.

    Journal: Genome Biology

    Article Title: An optimized microarray platform for assaying genomic variation in Plasmodium falciparum field populations

    doi: 10.1186/gb-2011-12-4-r35

    Figure Lengend Snippet: Field sample analysis with the CNV-SNP array . (a) The manufacturer-recommended starting amount is 1,000 ng of DNA to produce at least 10 μg of labeled product. However, 250 ng of parasite DNA consistently produced sufficient labeled product when using 65% AT nonamers. Error bars indicate one standard deviation. (b) Hybridizations with field samples - straight from patient blood, or whole genome amplified - produced microarray data on par with standard lab clones, even when significant human DNA contamination was present. Microarray accuracy was determined through Illumina sequencing of lab-adapted parasites. Patient blood samples were hybridized with the addition of 1× Denhardt's solution while WGA samples were not.

    Article Snippet: Labeled product was resuspended in water, and 10 μg of test and reference samples combined (6 μg for 5K SNP chip samples), dried down, and resuspended in hybridization buffer (Roche NimbleGen, Inc., Madison, WI, USA); hybridizations for patient blood samples included 1× Denhardt's solution (Sigma Aldrich) in the hybridization buffer.

    Techniques: Labeling, Produced, Standard Deviation, Amplification, Microarray, Clone Assay, Sequencing, Whole Genome Amplification

    Hybridization and detection of PCR amplicons relative to rRNA. Two micrograms of G. chapellei total RNA (approximately 2.4 × 10 12 copies of 16S rRNA) or 10 12 copies of a full-length G. chapellei rDNA PCR product (1,400 bp) were hybridized to a microarray overnight at room temperature (4× SSPE, 2.5× Denhardt's solution, 30% formamide), with or without a proximal chaperone (targeting the 214 region). In the absence of a chaperone, no rRNA was detected, as described in the text. The increased signal for the amplicon-plus-chaperone hybridizations is most likely due to the additive effect of two biotin labels; one on the PCR amplicon and one on the proximal chaperone.

    Journal: Applied and Environmental Microbiology

    Article Title: Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays

    doi: 10.1128/AEM.67.10.4708-4716.2001

    Figure Lengend Snippet: Hybridization and detection of PCR amplicons relative to rRNA. Two micrograms of G. chapellei total RNA (approximately 2.4 × 10 12 copies of 16S rRNA) or 10 12 copies of a full-length G. chapellei rDNA PCR product (1,400 bp) were hybridized to a microarray overnight at room temperature (4× SSPE, 2.5× Denhardt's solution, 30% formamide), with or without a proximal chaperone (targeting the 214 region). In the absence of a chaperone, no rRNA was detected, as described in the text. The increased signal for the amplicon-plus-chaperone hybridizations is most likely due to the additive effect of two biotin labels; one on the PCR amplicon and one on the proximal chaperone.

    Article Snippet: The resulting (dark-brown) pellet was washed in 70% ethanol, dried under a vacuum, resuspended in hybridization buffer (4× SSPE [1× SSPE is 0.18 M NaCl, 10 mM NaH2 PO4 , and 1 mM EDTA {pH 7.7}]–2.5× Denhardt's solution [50× Denhardt's solution is 10 g of Ficoll 400 {Sigma, St. Louis, Mo.} liter−1 , 10 g of polyvinylpyrrolidone (Sigma) liter−1 , and 10 g of ultrapure bovine serum albumin {Ambion} liter−1 ], with or without 30% formamide), and stored at −20°C until use.

    Techniques: Hybridization, Polymerase Chain Reaction, Microarray, Amplification

    Optimizing 16S rRNA detection sensitivity. Two micrograms of total RNA was hybridized in 4× SSPE–2.5× Denhardt's solution (pH 7.7) under the conditions indicated.

    Journal: Applied and Environmental Microbiology

    Article Title: Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays

    doi: 10.1128/AEM.67.10.4708-4716.2001

    Figure Lengend Snippet: Optimizing 16S rRNA detection sensitivity. Two micrograms of total RNA was hybridized in 4× SSPE–2.5× Denhardt's solution (pH 7.7) under the conditions indicated.

    Article Snippet: The resulting (dark-brown) pellet was washed in 70% ethanol, dried under a vacuum, resuspended in hybridization buffer (4× SSPE [1× SSPE is 0.18 M NaCl, 10 mM NaH2 PO4 , and 1 mM EDTA {pH 7.7}]–2.5× Denhardt's solution [50× Denhardt's solution is 10 g of Ficoll 400 {Sigma, St. Louis, Mo.} liter−1 , 10 g of polyvinylpyrrolidone (Sigma) liter−1 , and 10 g of ultrapure bovine serum albumin {Ambion} liter−1 ], with or without 30% formamide), and stored at −20°C until use.

    Techniques: