Journal: PLoS ONE

Article Title: Electrical Remodeling of Preoptic GABAergic Neurons Involves the Kv1.5 Subunit

doi: 10.1371/journal.pone.0096643

Figure Lengend Snippet: A. Hongotoxin-1 (0.3 nM) -sensitive component of I DR . Representative currents from a GAD65-GFP (left) and a Kv4.2−/−;GAD65-GFP (right) neuron. B. DPO-1 (0.5 µM) - sensitive component of I DR . Representative currents from a GAD65-GFP (left) and a Kv4.2−/−;GAD65-GFP (right) neuron. C. Margatoxin (5 nM) - sensitive component of I DR . Representative currents from a GAD65-GFP (left) and a Kv4.2−/−;GAD65-GFP (right) neuron. A-C. The currents were elicited by the same voltage step protocol depicted in the inset. TTX (1 µM) was present in all extracellular solutions. D. Bar chart summarizing the current density of the hongotoxin-1 (0.3 nM, n = 13 and n = 16 neurons, respectively)-sensitive, DPO-1 (0.5 µM, n = 23 and n = 26 neurons, respectively)-sensitive, dendrotoxin-K (50 nM, n = 5 and n = 6 neurons, respectively)-sensitive, and margatoxin (5 nM, n = 7 and n = 6 neurons, respectively)-sensitive in GAD65-GFP and Kv4.2−/−;GAD65-GFP neurons. Bars represent averages + S.D. The current densities were calculated using the I DR amplitude at the end of the depolarizing step to 0 mV. ** represents significant differences between GAD65-GFP and Kv4.2−/−;GAD65-GFP groups P<0.01 (t-test).

Article Snippet: DPO-1 and TTX were from Tocris (Ellisville, MO), dendrotoxin-K, hongotoxin-1, margatoxin, were from Alomone Labs (Jerusalem, Israel), while the other substances were purchased from Sigma.

Techniques:

Journal: eLife

Article Title: Kv1.1 channels regulate early postnatal neurogenesis in mouse hippocampus via the TrkB signaling pathway

doi: 10.7554/eLife.58779

Figure Lengend Snippet: ( A ) In situ hybridization results showed that Kcna1 mRNA is expressed in Fezf2-GFP-positive neural progenitor cells. Inset is displayed at a higher magnification. ( B ) Kcna1 mRNA was not detected in the Kv1.1KO mouse, which served as a negative control. ( C ) Kv1.1 protein was expressed in the doublecortin (DCX)-expressing late-stage neural progenitor cells (arrows) but not Sox2-positive early-stage neural progenitor cells. Kv1.1 protein was highly expressed in the inhibitory interneurons (yellow arrows). ( D–J ) Pharmacological isolation of Kv1 currents in Fezf2-GFP-positive cells. Kv1.1 currents were elicited by trains of voltage steps from -80 mV to +40 mV in 10 mV increments from the holding potential of -80 mV in the absence ( D and G ) or presence of the Kv1-specific blocker dendrotoxin-k (DTX-K; 100 nM) ( E and H ). The DTX-K-sensitive currents were considered Kv1-mediated potassium currents ( F, I, and J ), which were much reduced in the Kv1.1KO mice. n = 4 cells from each phenotype. Scale bar = 20 μm in ( C ). Data are presented as mean ± SEM.

Article Snippet: Dendrotoxin-κ (Alomone Labs, Israel) was used to specifically block the Kv1.1 channel.

Techniques: In Situ Hybridization, Negative Control, Expressing, Isolation

Journal: eLife

Article Title: Kv1.1 channels regulate early postnatal neurogenesis in mouse hippocampus via the TrkB signaling pathway

doi: 10.7554/eLife.58779

Figure Lengend Snippet:

Article Snippet: Dendrotoxin-κ (Alomone Labs, Israel) was used to specifically block the Kv1.1 channel.

Techniques: Multiplex Assay, Recombinant, Plasmid Preparation, Transferring, Software

Journal: Cell Reports

Article Title: Large, Stable Spikes Exhibit Differential Broadening in Excitatory and Inhibitory Neocortical Boutons

doi: 10.1016/j.celrep.2020.108612

Figure Lengend Snippet: Presynaptic Spike Amplitude Is Independent of Potassium Channels and Stable during Synaptic Scaling (A) Left: example action potential recorded from cultured boutons with 100 nM DTX-K included in the bath perfusion. Right: example action potential recorded from cultured boutons with 100 nM DTX-K + 1 mM TEA included in the bath perfusion. Representative control action potential recorded with quartz pipettes at physiological temperature (black, same as in Figure 5 B) is provided for comparison. Traces were aligned to the point of steepest rise during depolarization. (B) Left: peak potential of action potentials recorded under the conditions illustrated in (A). Right: half-duration of action potentials recorded under the conditions illustrated in (A) (color code as in A; bar graphs as mean ± SEM; n indicates number of recordings from individual boutons). (C) Example action potentials recorded from cultured boutons under control conditions (same as in Figure 5 B ) and after 48 h exposure to 1 μM TTX or 100 μM PTX (from left to right, respectively). (D) Left: overshoot of action potentials recorded under the conditions illustrated in (C). Right: half-duration of action potentials recorded under the conditions illustrated in (C) (color code as in C; bar graphs as mean ± SEM; n indicates number of recordings from individual boutons; control data same as in Figure 5 E). See also Figure S5 .

Article Snippet: DTX-K , Alomone Labs , Cat# D-400.

Techniques: Cell Culture

Journal: Cell Reports

Article Title: Large, Stable Spikes Exhibit Differential Broadening in Excitatory and Inhibitory Neocortical Boutons

doi: 10.1016/j.celrep.2020.108612

Figure Lengend Snippet:

Article Snippet: DTX-K , Alomone Labs , Cat# D-400.

Techniques: Recombinant, Software