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TaKaRa dde i
Dde I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dde i/product/TaKaRa
Average 99 stars, based on 3 article reviews
Price from $9.99 to $1999.99
dde i - by Bioz Stars, 2020-02
99/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA
Article Snippet: .. The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker. .. Nested RT-PCR The RT reaction was carried out using 1 μL RNA sample and the ThermoScript RT-PCR system (Thermo Fisher Scientific) according to the manufacturers’ protocol.

Multiplex Assay:

Article Title: Female genetic distribution bias in mitochondrial genome observed in Parkinson’s Disease patients in northern China
Article Snippet: Two multiplex and one single PCR were performed under the following cycle conditions: initial denaturation of 94 °C for 1 min, followed by 30 cycles (for system 1) or 35 cycles (for system 2 and 3) of 94 °C, denaturation for 30 s, 55 °C annealing for 30 s, and 72 °C elongation for 30 s, followed by a final extension at 72 °C for 1 min. .. Amplification products from system 1 and 0.25 μl Dde I (TaKaRa, Dalian, China) were mixed in 10 μl TaKaRa K buffer.

Amplification:

Article Title: Female genetic distribution bias in mitochondrial genome observed in Parkinson’s Disease patients in northern China
Article Snippet: .. Amplification products from system 1 and 0.25 μl Dde I (TaKaRa, Dalian, China) were mixed in 10 μl TaKaRa K buffer. .. Amplification products from systems 2 and 3 and 0.25 μl Hae II and Nae I (TaKaRa, Dalian, China) were mixed in 10 μl TaKaRa M buffer.

Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA
Article Snippet: RT-LAMP The RT-LAMP assay was carried out using the Loopamp RNA Amplification kit (Eiken Chemical, Tokyo, Japan) under the following conditions: 1 μL sample (RNA or DNA) was mixed with 20 pmol each of FIP and BIP primers, 10 pmol each of LF and LB primers, 2.5 pmol each of F3 and B3 primers, 1 μL Enzyme Mix, and 12.5 μL of 2 × Reaction Mix, with distilled water added to obtain a final volume of 25 μL. .. The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker.

Agarose Gel Electrophoresis:

Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA
Article Snippet: .. The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker. .. Nested RT-PCR The RT reaction was carried out using 1 μL RNA sample and the ThermoScript RT-PCR system (Thermo Fisher Scientific) according to the manufacturers’ protocol.

RT Lamp Assay:

Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA
Article Snippet: RT-LAMP The RT-LAMP assay was carried out using the Loopamp RNA Amplification kit (Eiken Chemical, Tokyo, Japan) under the following conditions: 1 μL sample (RNA or DNA) was mixed with 20 pmol each of FIP and BIP primers, 10 pmol each of LF and LB primers, 2.5 pmol each of F3 and B3 primers, 1 μL Enzyme Mix, and 12.5 μL of 2 × Reaction Mix, with distilled water added to obtain a final volume of 25 μL. .. The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker.

Incubation:

Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA
Article Snippet: .. The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker. .. Nested RT-PCR The RT reaction was carried out using 1 μL RNA sample and the ThermoScript RT-PCR system (Thermo Fisher Scientific) according to the manufacturers’ protocol.

Marker:

Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA
Article Snippet: .. The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker. .. Nested RT-PCR The RT reaction was carried out using 1 μL RNA sample and the ThermoScript RT-PCR system (Thermo Fisher Scientific) according to the manufacturers’ protocol.

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    TaKaRa dde i
    Schematic representation of primer design for detection of PML-RAR α mRNA by <t>RT-LAMP</t> and cutting sites of <t>Dde</t> I restriction enzyme assay. Exon numbers of the PML gene (dark) and the RAR α gene (light) are shown. PML , promyelocytic leukemia ; RAR α, retinoic acid receptor α; RT-LAMP, reverse transcription loop-mediated isothermal amplification.
    Dde I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde i/product/TaKaRa
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    dde i - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

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    Schematic representation of primer design for detection of PML-RAR α mRNA by RT-LAMP and cutting sites of Dde I restriction enzyme assay. Exon numbers of the PML gene (dark) and the RAR α gene (light) are shown. PML , promyelocytic leukemia ; RAR α, retinoic acid receptor α; RT-LAMP, reverse transcription loop-mediated isothermal amplification.

    Journal: Yonago Acta Medica

    Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA

    doi:

    Figure Lengend Snippet: Schematic representation of primer design for detection of PML-RAR α mRNA by RT-LAMP and cutting sites of Dde I restriction enzyme assay. Exon numbers of the PML gene (dark) and the RAR α gene (light) are shown. PML , promyelocytic leukemia ; RAR α, retinoic acid receptor α; RT-LAMP, reverse transcription loop-mediated isothermal amplification.

    Article Snippet: The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker.

    Techniques: Enzymatic Assay, Amplification

    Dde I restriction enzyme digestion of RT-LAMP products. Products of bcr1, bcr2, and bcr3 plasmid DNA amplification were incubated with (+) or without (−) Dde I at 37 °C for 120 min, followed by deactivation at 70 °C for 15 min and separation by 1.5% agarose gel electrophoresis (100 V, 35 min). The amounts of bcr1, bcr2 and bcr3 plasmid DNAs were 8.8, 7.8, and 8.0 × 10 6 copies/tube, respectively. The expected sizes of digested RT-LAMP products are the followings: 468 bp, about 234 bp (stem and loop structure) and 83 bp for bcr1; 232 bp, about 116 bp (stem and loop structure) and 83 bp for bcr2; and 139 bp and 121 bp (merged in Figure) for bcr3. DW, distilled water (negative control); M, 100-bp ladder size marker; neg RNA, IVS-0035 RNA (negative control); RT-LAMP, reverse transcription loop-mediated isothermal amplification.

    Journal: Yonago Acta Medica

    Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA

    doi:

    Figure Lengend Snippet: Dde I restriction enzyme digestion of RT-LAMP products. Products of bcr1, bcr2, and bcr3 plasmid DNA amplification were incubated with (+) or without (−) Dde I at 37 °C for 120 min, followed by deactivation at 70 °C for 15 min and separation by 1.5% agarose gel electrophoresis (100 V, 35 min). The amounts of bcr1, bcr2 and bcr3 plasmid DNAs were 8.8, 7.8, and 8.0 × 10 6 copies/tube, respectively. The expected sizes of digested RT-LAMP products are the followings: 468 bp, about 234 bp (stem and loop structure) and 83 bp for bcr1; 232 bp, about 116 bp (stem and loop structure) and 83 bp for bcr2; and 139 bp and 121 bp (merged in Figure) for bcr3. DW, distilled water (negative control); M, 100-bp ladder size marker; neg RNA, IVS-0035 RNA (negative control); RT-LAMP, reverse transcription loop-mediated isothermal amplification.

    Article Snippet: The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker.

    Techniques: Plasmid Preparation, Amplification, Incubation, Agarose Gel Electrophoresis, Negative Control, Marker