dcv infected drosophila s2 cells  (Thermo Fisher)


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    Thermo Fisher dcv infected drosophila s2 cells
    The heat shock response is dynamic and requires viral replication in Drosophila S2 cells. Expression of the genes encoding the heat shock proteins Hsp70, Hsp23, Hsp26, and the Heat shock transcription factor (Hsf) was monitored at the indicated time points by RT-qPCR after infection with ( a ) <t>DCV,</t> ( b ) CrPV or ( c ) IIV-6 (MOI = 10). ( d ) <t>S2</t> cells were inoculated with UV-inactivated viruses and gene expression was measured at 24, 16 or 48 hpi with DCV, CrPV and IIV-6, respectively. Expression of the gene of interest was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to mock infection. Data are mean and s.d. of three independent infections. Student’s t-tests were used to compare virus-infected samples to mock infections (* P
    Dcv Infected Drosophila S2 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dcv infected drosophila s2 cells/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dcv infected drosophila s2 cells - by Bioz Stars, 2022-10
    99/100 stars

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    1) Product Images from "The heat shock response restricts virus infection in Drosophila"

    Article Title: The heat shock response restricts virus infection in Drosophila

    Journal: Scientific Reports

    doi: 10.1038/srep12758

    The heat shock response is dynamic and requires viral replication in Drosophila S2 cells. Expression of the genes encoding the heat shock proteins Hsp70, Hsp23, Hsp26, and the Heat shock transcription factor (Hsf) was monitored at the indicated time points by RT-qPCR after infection with ( a ) DCV, ( b ) CrPV or ( c ) IIV-6 (MOI = 10). ( d ) S2 cells were inoculated with UV-inactivated viruses and gene expression was measured at 24, 16 or 48 hpi with DCV, CrPV and IIV-6, respectively. Expression of the gene of interest was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to mock infection. Data are mean and s.d. of three independent infections. Student’s t-tests were used to compare virus-infected samples to mock infections (* P
    Figure Legend Snippet: The heat shock response is dynamic and requires viral replication in Drosophila S2 cells. Expression of the genes encoding the heat shock proteins Hsp70, Hsp23, Hsp26, and the Heat shock transcription factor (Hsf) was monitored at the indicated time points by RT-qPCR after infection with ( a ) DCV, ( b ) CrPV or ( c ) IIV-6 (MOI = 10). ( d ) S2 cells were inoculated with UV-inactivated viruses and gene expression was measured at 24, 16 or 48 hpi with DCV, CrPV and IIV-6, respectively. Expression of the gene of interest was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to mock infection. Data are mean and s.d. of three independent infections. Student’s t-tests were used to compare virus-infected samples to mock infections (* P

    Techniques Used: Expressing, Quantitative RT-PCR, Infection

    Microarray analysis of DCV-infected Drosophila S2 cells. ( a ) Overview of the experimental workflow. S2 cells were infected with DCV (MOI = 10) or mock-infected with Schneider’s medium, and RNA was extracted at 8 and 24 hours post-infection (hpi) for microarray analyses. Figure drawn by S.H. Merkling. ( b ) Number of differentially expressed genes at 8 and 24 hpi (fold change ≥2 relative to mock infection). ( c ) Venn diagram representing the overlap between differentially induced genes after DCV infection at 8 and 24 hpi. ( d , e ) Gene ontology (GO) analysis of the genes that are upregulated ≥2-fold at ( d ) 8 hpi and ( e ) 24 hpi. All significantly enriched level 4 GO terms are shown ( P
    Figure Legend Snippet: Microarray analysis of DCV-infected Drosophila S2 cells. ( a ) Overview of the experimental workflow. S2 cells were infected with DCV (MOI = 10) or mock-infected with Schneider’s medium, and RNA was extracted at 8 and 24 hours post-infection (hpi) for microarray analyses. Figure drawn by S.H. Merkling. ( b ) Number of differentially expressed genes at 8 and 24 hpi (fold change ≥2 relative to mock infection). ( c ) Venn diagram representing the overlap between differentially induced genes after DCV infection at 8 and 24 hpi. ( d , e ) Gene ontology (GO) analysis of the genes that are upregulated ≥2-fold at ( d ) 8 hpi and ( e ) 24 hpi. All significantly enriched level 4 GO terms are shown ( P

    Techniques Used: Microarray, Infection

    2) Product Images from "The heat shock response restricts virus infection in Drosophila"

    Article Title: The heat shock response restricts virus infection in Drosophila

    Journal: Scientific Reports

    doi: 10.1038/srep12758

    The heat shock response is dynamic and requires viral replication in Drosophila S2 cells. Expression of the genes encoding the heat shock proteins Hsp70, Hsp23, Hsp26, and the Heat shock transcription factor (Hsf) was monitored at the indicated time points by RT-qPCR after infection with ( a ) DCV, ( b ) CrPV or ( c ) IIV-6 (MOI = 10). ( d ) S2 cells were inoculated with UV-inactivated viruses and gene expression was measured at 24, 16 or 48 hpi with DCV, CrPV and IIV-6, respectively. Expression of the gene of interest was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to mock infection. Data are mean and s.d. of three independent infections. Student’s t-tests were used to compare virus-infected samples to mock infections (* P
    Figure Legend Snippet: The heat shock response is dynamic and requires viral replication in Drosophila S2 cells. Expression of the genes encoding the heat shock proteins Hsp70, Hsp23, Hsp26, and the Heat shock transcription factor (Hsf) was monitored at the indicated time points by RT-qPCR after infection with ( a ) DCV, ( b ) CrPV or ( c ) IIV-6 (MOI = 10). ( d ) S2 cells were inoculated with UV-inactivated viruses and gene expression was measured at 24, 16 or 48 hpi with DCV, CrPV and IIV-6, respectively. Expression of the gene of interest was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to mock infection. Data are mean and s.d. of three independent infections. Student’s t-tests were used to compare virus-infected samples to mock infections (* P

    Techniques Used: Expressing, Quantitative RT-PCR, Infection

    Microarray analysis of DCV-infected Drosophila S2 cells. ( a ) Overview of the experimental workflow. S2 cells were infected with DCV (MOI = 10) or mock-infected with Schneider’s medium, and RNA was extracted at 8 and 24 hours post-infection (hpi) for microarray analyses. Figure drawn by S.H. Merkling. ( b ) Number of differentially expressed genes at 8 and 24 hpi (fold change ≥2 relative to mock infection). ( c ) Venn diagram representing the overlap between differentially induced genes after DCV infection at 8 and 24 hpi. ( d , e ) Gene ontology (GO) analysis of the genes that are upregulated ≥2-fold at ( d ) 8 hpi and ( e ) 24 hpi. All significantly enriched level 4 GO terms are shown ( P
    Figure Legend Snippet: Microarray analysis of DCV-infected Drosophila S2 cells. ( a ) Overview of the experimental workflow. S2 cells were infected with DCV (MOI = 10) or mock-infected with Schneider’s medium, and RNA was extracted at 8 and 24 hours post-infection (hpi) for microarray analyses. Figure drawn by S.H. Merkling. ( b ) Number of differentially expressed genes at 8 and 24 hpi (fold change ≥2 relative to mock infection). ( c ) Venn diagram representing the overlap between differentially induced genes after DCV infection at 8 and 24 hpi. ( d , e ) Gene ontology (GO) analysis of the genes that are upregulated ≥2-fold at ( d ) 8 hpi and ( e ) 24 hpi. All significantly enriched level 4 GO terms are shown ( P

    Techniques Used: Microarray, Infection

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    Thermo Fisher dcv infected drosophila s2 cells
    The heat shock response is dynamic and requires viral replication in Drosophila S2 cells. Expression of the genes encoding the heat shock proteins Hsp70, Hsp23, Hsp26, and the Heat shock transcription factor (Hsf) was monitored at the indicated time points by RT-qPCR after infection with ( a ) <t>DCV,</t> ( b ) CrPV or ( c ) IIV-6 (MOI = 10). ( d ) <t>S2</t> cells were inoculated with UV-inactivated viruses and gene expression was measured at 24, 16 or 48 hpi with DCV, CrPV and IIV-6, respectively. Expression of the gene of interest was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to mock infection. Data are mean and s.d. of three independent infections. Student’s t-tests were used to compare virus-infected samples to mock infections (* P
    Dcv Infected Drosophila S2 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dcv infected drosophila s2 cells/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dcv infected drosophila s2 cells - by Bioz Stars, 2022-10
    99/100 stars
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    The heat shock response is dynamic and requires viral replication in Drosophila S2 cells. Expression of the genes encoding the heat shock proteins Hsp70, Hsp23, Hsp26, and the Heat shock transcription factor (Hsf) was monitored at the indicated time points by RT-qPCR after infection with ( a ) DCV, ( b ) CrPV or ( c ) IIV-6 (MOI = 10). ( d ) S2 cells were inoculated with UV-inactivated viruses and gene expression was measured at 24, 16 or 48 hpi with DCV, CrPV and IIV-6, respectively. Expression of the gene of interest was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to mock infection. Data are mean and s.d. of three independent infections. Student’s t-tests were used to compare virus-infected samples to mock infections (* P

    Journal: Scientific Reports

    Article Title: The heat shock response restricts virus infection in Drosophila

    doi: 10.1038/srep12758

    Figure Lengend Snippet: The heat shock response is dynamic and requires viral replication in Drosophila S2 cells. Expression of the genes encoding the heat shock proteins Hsp70, Hsp23, Hsp26, and the Heat shock transcription factor (Hsf) was monitored at the indicated time points by RT-qPCR after infection with ( a ) DCV, ( b ) CrPV or ( c ) IIV-6 (MOI = 10). ( d ) S2 cells were inoculated with UV-inactivated viruses and gene expression was measured at 24, 16 or 48 hpi with DCV, CrPV and IIV-6, respectively. Expression of the gene of interest was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to mock infection. Data are mean and s.d. of three independent infections. Student’s t-tests were used to compare virus-infected samples to mock infections (* P

    Article Snippet: The heat shock response is induced in DCV-infected Drosophila S2 cells To identify novel factors or processes involved in antiviral defence in Drosophila , we generated transcriptional profiles of DCV-infected Drosophila S2 cells at 8 and 24 hours post-infection (hpi) using Affymetrix GeneChip microarrays ( ).

    Techniques: Expressing, Quantitative RT-PCR, Infection

    Microarray analysis of DCV-infected Drosophila S2 cells. ( a ) Overview of the experimental workflow. S2 cells were infected with DCV (MOI = 10) or mock-infected with Schneider’s medium, and RNA was extracted at 8 and 24 hours post-infection (hpi) for microarray analyses. Figure drawn by S.H. Merkling. ( b ) Number of differentially expressed genes at 8 and 24 hpi (fold change ≥2 relative to mock infection). ( c ) Venn diagram representing the overlap between differentially induced genes after DCV infection at 8 and 24 hpi. ( d , e ) Gene ontology (GO) analysis of the genes that are upregulated ≥2-fold at ( d ) 8 hpi and ( e ) 24 hpi. All significantly enriched level 4 GO terms are shown ( P

    Journal: Scientific Reports

    Article Title: The heat shock response restricts virus infection in Drosophila

    doi: 10.1038/srep12758

    Figure Lengend Snippet: Microarray analysis of DCV-infected Drosophila S2 cells. ( a ) Overview of the experimental workflow. S2 cells were infected with DCV (MOI = 10) or mock-infected with Schneider’s medium, and RNA was extracted at 8 and 24 hours post-infection (hpi) for microarray analyses. Figure drawn by S.H. Merkling. ( b ) Number of differentially expressed genes at 8 and 24 hpi (fold change ≥2 relative to mock infection). ( c ) Venn diagram representing the overlap between differentially induced genes after DCV infection at 8 and 24 hpi. ( d , e ) Gene ontology (GO) analysis of the genes that are upregulated ≥2-fold at ( d ) 8 hpi and ( e ) 24 hpi. All significantly enriched level 4 GO terms are shown ( P

    Article Snippet: The heat shock response is induced in DCV-infected Drosophila S2 cells To identify novel factors or processes involved in antiviral defence in Drosophila , we generated transcriptional profiles of DCV-infected Drosophila S2 cells at 8 and 24 hours post-infection (hpi) using Affymetrix GeneChip microarrays ( ).

    Techniques: Microarray, Infection