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Click Chemistry Tools dbco s s peg3 biotin
(a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
Dbco S S Peg3 Biotin, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dbco s s peg3 biotin/product/Click Chemistry Tools
Average 87 stars, based on 3 article reviews
Price from $9.99 to $1999.99
dbco s s peg3 biotin - by Bioz Stars, 2020-01
87/100 stars

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1) Product Images from "O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry"

Article Title: O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry

Journal: Analytical chemistry

doi: 10.1021/acs.analchem.8b05688

(a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
Figure Legend Snippet: (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).

Techniques Used: Labeling

Related Articles

Centrifugation:

Article Title: Bioorthogonal labeling cell-surface proteins expressed in pancreatic cancer cells to identify potential diagnostic/therapeutic biomarkers
Article Snippet: After 72 h, the cells were washed twice with phosphate-buffered saline (PBS) then treated with culture medium containing 200 µM of either EZ-Link Phosphine-PEG3 -Biotin (Thermo Fisher Scientific, 88901) or DBCO-S-S-PEG3-Biotin (Click Chemistry Tools, A112) conjugate suspended in DMSO to selectively tag glycoproteins on the surface of live cells. .. The cell suspensions from each sample (azido sugar or control sugar) were pooled, collected by centrifugation, washed twice with PBS, and lysed with RIPA buffer (1 mM EDTA, 1% NP-40, 0.5% deoxycholate, 0.1% SDS in PBS) followed by sonication.

Polymerase Chain Reaction:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

Mutagenesis:

Article Title: O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry
Article Snippet: Specifically, gCTD, α -crystallin digest, and TAB2 digest in 100 μ /L of 50 mM HEPES (pH 7.9) were chemoenzymatically labeled by incubating overnight with UDP–GalNAz (25 μ L; Life Technologies) and GalT1 mutant (15 μ L; Life Technologies) in the presence of 22 μ L of MgCl2 (Life Technologies) at 4 °C, according to the manufacturer instructions and our previous papers. .. Copper-free click chemistry was performed in 15 μ L of PBS and 4 μ L of 1 mM DBCO–S–S–PEG3–biotin (predissolved in DMSO; Click Chemistry Tools LLC) for 2 h at room temperature.

Labeling:

Article Title: Bioorthogonal labeling cell-surface proteins expressed in pancreatic cancer cells to identify potential diagnostic/therapeutic biomarkers
Article Snippet: Paragraph title: Metabolic labeling cells ... After 72 h, the cells were washed twice with phosphate-buffered saline (PBS) then treated with culture medium containing 200 µM of either EZ-Link Phosphine-PEG3 -Biotin (Thermo Fisher Scientific, 88901) or DBCO-S-S-PEG3-Biotin (Click Chemistry Tools, A112) conjugate suspended in DMSO to selectively tag glycoproteins on the surface of live cells.

Article Title: O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry
Article Snippet: Paragraph title: Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Derivatization of O-GlcNAc Peptides. ... Copper-free click chemistry was performed in 15 μ L of PBS and 4 μ L of 1 mM DBCO–S–S–PEG3–biotin (predissolved in DMSO; Click Chemistry Tools LLC) for 2 h at room temperature.

Purification:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

Concentration Assay:

Article Title: Bioorthogonal labeling cell-surface proteins expressed in pancreatic cancer cells to identify potential diagnostic/therapeutic biomarkers
Article Snippet: The following day, 10 mM N -azidoacetylmannosamine-tetraacylated prepared in DMSO (Ac4 ManNAz, Thermo Fisher Scientific, 88904) was added to 2 dishes of cells to yield a final concentration of 50 µM azido sugar. .. After 72 h, the cells were washed twice with phosphate-buffered saline (PBS) then treated with culture medium containing 200 µM of either EZ-Link Phosphine-PEG3 -Biotin (Thermo Fisher Scientific, 88901) or DBCO-S-S-PEG3-Biotin (Click Chemistry Tools, A112) conjugate suspended in DMSO to selectively tag glycoproteins on the surface of live cells.

Incubation:

Article Title: O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry
Article Snippet: – Calf intestine phosphatase (20 U; New England Biolabs) was then added and incubated for 3 h at room temperature. .. Copper-free click chemistry was performed in 15 μ L of PBS and 4 μ L of 1 mM DBCO–S–S–PEG3–biotin (predissolved in DMSO; Click Chemistry Tools LLC) for 2 h at room temperature.

DNA Purification:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

Marker:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

Sonication:

Article Title: Bioorthogonal labeling cell-surface proteins expressed in pancreatic cancer cells to identify potential diagnostic/therapeutic biomarkers
Article Snippet: After 72 h, the cells were washed twice with phosphate-buffered saline (PBS) then treated with culture medium containing 200 µM of either EZ-Link Phosphine-PEG3 -Biotin (Thermo Fisher Scientific, 88901) or DBCO-S-S-PEG3-Biotin (Click Chemistry Tools, A112) conjugate suspended in DMSO to selectively tag glycoproteins on the surface of live cells. .. The cell suspensions from each sample (azido sugar or control sugar) were pooled, collected by centrifugation, washed twice with PBS, and lysed with RIPA buffer (1 mM EDTA, 1% NP-40, 0.5% deoxycholate, 0.1% SDS in PBS) followed by sonication.

Binding Assay:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

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    Click Chemistry Tools dbco s s peg3 biotin
    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
    Dbco S S Peg3 Biotin, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dbco s s peg3 biotin/product/Click Chemistry Tools
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dbco s s peg3 biotin - by Bioz Stars, 2020-01
    87/100 stars
      Buy from Supplier

    79
    Click Chemistry Tools disulfide biotin linker
    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
    Disulfide Biotin Linker, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disulfide biotin linker/product/Click Chemistry Tools
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    disulfide biotin linker - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    Image Search Results


    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).

    Journal: Analytical chemistry

    Article Title: O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry

    doi: 10.1021/acs.analchem.8b05688

    Figure Lengend Snippet: (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).

    Article Snippet: Copper-free click chemistry was performed in 15 μ L of PBS and 4 μ L of 1 mM DBCO–S–S–PEG3–biotin (predissolved in DMSO; Click Chemistry Tools LLC) for 2 h at room temperature.

    Techniques: Labeling