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Click Chemistry Tools dbco s s peg3 biotin
(a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
Dbco S S Peg3 Biotin, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dbco s s peg3 biotin/product/Click Chemistry Tools
Average 88 stars, based on 4 article reviews
Price from $9.99 to $1999.99
dbco s s peg3 biotin - by Bioz Stars, 2020-09
88/100 stars

Images

1) Product Images from "O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry"

Article Title: O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry

Journal: Analytical chemistry

doi: 10.1021/acs.analchem.8b05688

(a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
Figure Legend Snippet: (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).

Techniques Used: Labeling

Related Articles

Polymerase Chain Reaction:

Article Title: Bisulfite-free and Base-resolution Analysis of 5-formylcytosine at Whole-genome Scale
Article Snippet: .. Click chemistry was performed by adding the DBCO-S-S-PEG3 -Biotin (Click Chemistry Tools, Cat. No. A112-10) to a final concentration of 400 mM and incubating at 37°C for 2 h. Purification steps were performed with QIAquick PCR Purification Kit. .. The precipitated DNA was again applied to Micro Bio-Spin P-6 Gel Columns (Bio-Rad) to remove any additional chemicals.

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

Purification:

Article Title: Bisulfite-free and Base-resolution Analysis of 5-formylcytosine at Whole-genome Scale
Article Snippet: .. Click chemistry was performed by adding the DBCO-S-S-PEG3 -Biotin (Click Chemistry Tools, Cat. No. A112-10) to a final concentration of 400 mM and incubating at 37°C for 2 h. Purification steps were performed with QIAquick PCR Purification Kit. .. The precipitated DNA was again applied to Micro Bio-Spin P-6 Gel Columns (Bio-Rad) to remove any additional chemicals.

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

Concentration Assay:

Article Title: Bisulfite-free and Base-resolution Analysis of 5-formylcytosine at Whole-genome Scale
Article Snippet: .. Click chemistry was performed by adding the DBCO-S-S-PEG3 -Biotin (Click Chemistry Tools, Cat. No. A112-10) to a final concentration of 400 mM and incubating at 37°C for 2 h. Purification steps were performed with QIAquick PCR Purification Kit. .. The precipitated DNA was again applied to Micro Bio-Spin P-6 Gel Columns (Bio-Rad) to remove any additional chemicals.

other:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: Dissolve 10 mg DBCO-S-S-PEG3-Biotin conjugate in 384 µl DMSO to make 30 mM stock solution and store at −80 °C.

DNA Purification:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

Marker:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

Binding Assay:

Article Title: 5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Article Snippet: .. Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S3014) Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884) Trizma base (Tris) (Sigma-Aldrich, catalog number: T1503) HEPES (Genscript, catalog number: ) Magnesium chloride (MgCl2 ) (Sigma-Aldrich, catalog number: M8266) Dimethyl sulfoxide (DMSO) (Fisher Chemical, catalog number: D128) Tween 20 (Sigma-Aldrich, catalog number: P9416) DBCO-S-S-PEG3-Biotin conjugate (Click Chemistry Tools, catalog number: A112P3) 1, 4-Dithiothreitol, ultrapure (DTT) (Roche Applied Science, catalog number: 3117006001) Wizard Genomic DNA Purification Kit (Promega Corporation, catalog number: A1120) 10 kDa Amicon Ultra-0.5 ml centrifugal filters (EMD Millipore, catalog number: UFC501008) QIAquick Nucleotide Removal Kit (QIAGEN, catalog number: 28304) Dynabeads MyOne Streptavidin C1 (Life Technologies, Invitrogen™, catalog number: 650-01) Qiagen MinElute PCR Purification Kit (QIAGEN, catalog number: 28004) UltraPure Agarose (Life Technologies, Invitrogen™, catalog number: 16500500) β-glucosyltransferase (β-GT) (New England Biolabs) DNA marker 10x β-GT reaction buffer (see Recipes) 2x binding and washing (B & W) buffer (see Recipes) TE (see Recipes) .. Sonication device (Covaris) Desktop centrifuges Water bath (Thermo Fisher Scientific) Gel running apparatus (Bio-Rad Laboratories) Nanodrop 1000 (Thermo Fisher Scientific) Labquake tube shaker (Barnstead/Thermolyne) Magnetic separation stand (Promega Corporation, catalog number: Z5342) Qubit 2.0 Fluorometer (Life Technologies, Invitrogen™) Hydroxymethyl collector (Active Motif, catalog number: 55013) Micro Bio-Spin 6 column (Bio-Rad Laboratories, catalog number: 732-6222)

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    Click Chemistry Tools dbco s s peg3 biotin
    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
    Dbco S S Peg3 Biotin, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dbco s s peg3 biotin/product/Click Chemistry Tools
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dbco s s peg3 biotin - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    86
    Click Chemistry Tools disulfide biotin linker
    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
    Disulfide Biotin Linker, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disulfide biotin linker/product/Click Chemistry Tools
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    disulfide biotin linker - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    85
    Click Chemistry Tools dbco s s peg3 biotin conjugate
    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
    Dbco S S Peg3 Biotin Conjugate, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dbco s s peg3 biotin conjugate/product/Click Chemistry Tools
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dbco s s peg3 biotin conjugate - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).

    Journal: Analytical chemistry

    Article Title: O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry

    doi: 10.1021/acs.analchem.8b05688

    Figure Lengend Snippet: (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).

    Article Snippet: Copper-free click chemistry was performed in 15 μ L of PBS and 4 μ L of 1 mM DBCO–S–S–PEG3–biotin (predissolved in DMSO; Click Chemistry Tools LLC) for 2 h at room temperature.

    Techniques: Labeling