dibenzylcyclooctyne biotin  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne biotin
    Dibenzylcyclooctyne Biotin, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dibenzylcyclooctyne biotin  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne biotin
    Dibenzylcyclooctyne Biotin, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dibenzylcyclooctyne flag  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne flag
    Dibenzylcyclooctyne Flag, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dibenzocyclooctyl dbco peg4  (Jena Bioscience)


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    Jena Bioscience dibenzocyclooctyl dbco peg4

    Dibenzocyclooctyl Dbco Peg4, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Simultaneous global labeling (SiGL) of 5-methylcytosine and 5-hydroxymethylcytosine by DNA alkylation with a synthetic cofactor and engineered methyltransferase"

    Article Title: Simultaneous global labeling (SiGL) of 5-methylcytosine and 5-hydroxymethylcytosine by DNA alkylation with a synthetic cofactor and engineered methyltransferase

    Journal: bioRxiv

    doi: 10.1101/2022.10.31.513813


    Figure Legend Snippet:

    Techniques Used: Modification

    dbco peg 4 5  (Jena Bioscience)


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    Jena Bioscience dbco peg 4 5
    Dbco Peg 4 5, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dibenzylcyclooctyne peg4 biotin  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne peg4 biotin
    Dibenzylcyclooctyne Peg4 Biotin, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dibenzylcyclooctyne peg 4 5  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne peg 4 5
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    dibenzylcyclooctyne peg 4 nh 2 dbco  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne peg 4 nh 2 dbco
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    maleimide peg4 dbco linker  (Jena Bioscience)


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    Jena Bioscience maleimide peg4 dbco linker
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    dbco peg 4 5  (Jena Bioscience)


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    Jena Bioscience dbco peg 4 5
    Proof of coupling specificity and maintained bioactivity after a copper-free reaction of BMP2 Azide with a DBCO-functionalized fluorophore. ( A ) BMP2 Azide (20 µM concentration) was coupled to different concentrations of a DBCO-functionalized fluorophore (DBCO-Cy5). Coupling of BMP2 Azide to Cy5-DBCO was detected by fluorescence (upper panel). Gels were stained with Coomassie Brilliant Blue for loading control (lower panel). BMP2 WT was used as negative control; ( B ) Graphs show dose-dependent ligand-induced ALP expression in C2C12 cells by uncoupled BMP2 Azide (red line) and BMP2 Azide (20 µM) coupled to DBCO-functionalized fluorophore (DBCO-PEG 4 -5/6-Texas Red, 100 µM) after being incubated for different reaction times (15 min, 30 min, 60 min, 120 min and overnight). Relative phosphatase expression shows absorption at 405 nm after subtraction of the background absorption at 405 nm. Statistical analyses of the ALP data were performed using two-way ANOVA–Dunnett’s multiple comparisons test. The only significant differences were observed by comparing the ALP activities induced by uncoupled BMP2 Azide and those induced by coupled BMP2 Azide (15, 30, 60 and 120 min of coupling time) at 25 nM concentrations; p < 0.05. ( C ) Alkaline phosphatase staining in C2C12 cells incubated with 200 nM uncoupled BMP2 Azide and 200 nM BMP2 Azide coupled to DBCO-PEG 4 -5/6-Texas Red for 2 h. Microscopic images were taken 5 min after addition of NBT/BCIP.
    Dbco Peg 4 5, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Site-Directed Immobilization of an Engineered Bone Morphogenetic Protein 2 (BMP2) Variant to Collagen-Based Microspheres Induces Bone Formation In Vivo"

    Article Title: Site-Directed Immobilization of an Engineered Bone Morphogenetic Protein 2 (BMP2) Variant to Collagen-Based Microspheres Induces Bone Formation In Vivo

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23073928

    Proof of coupling specificity and maintained bioactivity after a copper-free reaction of BMP2 Azide with a DBCO-functionalized fluorophore. ( A ) BMP2 Azide (20 µM concentration) was coupled to different concentrations of a DBCO-functionalized fluorophore (DBCO-Cy5). Coupling of BMP2 Azide to Cy5-DBCO was detected by fluorescence (upper panel). Gels were stained with Coomassie Brilliant Blue for loading control (lower panel). BMP2 WT was used as negative control; ( B ) Graphs show dose-dependent ligand-induced ALP expression in C2C12 cells by uncoupled BMP2 Azide (red line) and BMP2 Azide (20 µM) coupled to DBCO-functionalized fluorophore (DBCO-PEG 4 -5/6-Texas Red, 100 µM) after being incubated for different reaction times (15 min, 30 min, 60 min, 120 min and overnight). Relative phosphatase expression shows absorption at 405 nm after subtraction of the background absorption at 405 nm. Statistical analyses of the ALP data were performed using two-way ANOVA–Dunnett’s multiple comparisons test. The only significant differences were observed by comparing the ALP activities induced by uncoupled BMP2 Azide and those induced by coupled BMP2 Azide (15, 30, 60 and 120 min of coupling time) at 25 nM concentrations; p < 0.05. ( C ) Alkaline phosphatase staining in C2C12 cells incubated with 200 nM uncoupled BMP2 Azide and 200 nM BMP2 Azide coupled to DBCO-PEG 4 -5/6-Texas Red for 2 h. Microscopic images were taken 5 min after addition of NBT/BCIP.
    Figure Legend Snippet: Proof of coupling specificity and maintained bioactivity after a copper-free reaction of BMP2 Azide with a DBCO-functionalized fluorophore. ( A ) BMP2 Azide (20 µM concentration) was coupled to different concentrations of a DBCO-functionalized fluorophore (DBCO-Cy5). Coupling of BMP2 Azide to Cy5-DBCO was detected by fluorescence (upper panel). Gels were stained with Coomassie Brilliant Blue for loading control (lower panel). BMP2 WT was used as negative control; ( B ) Graphs show dose-dependent ligand-induced ALP expression in C2C12 cells by uncoupled BMP2 Azide (red line) and BMP2 Azide (20 µM) coupled to DBCO-functionalized fluorophore (DBCO-PEG 4 -5/6-Texas Red, 100 µM) after being incubated for different reaction times (15 min, 30 min, 60 min, 120 min and overnight). Relative phosphatase expression shows absorption at 405 nm after subtraction of the background absorption at 405 nm. Statistical analyses of the ALP data were performed using two-way ANOVA–Dunnett’s multiple comparisons test. The only significant differences were observed by comparing the ALP activities induced by uncoupled BMP2 Azide and those induced by coupled BMP2 Azide (15, 30, 60 and 120 min of coupling time) at 25 nM concentrations; p < 0.05. ( C ) Alkaline phosphatase staining in C2C12 cells incubated with 200 nM uncoupled BMP2 Azide and 200 nM BMP2 Azide coupled to DBCO-PEG 4 -5/6-Texas Red for 2 h. Microscopic images were taken 5 min after addition of NBT/BCIP.

    Techniques Used: Concentration Assay, Fluorescence, Staining, Negative Control, Expressing, Incubation

    dbco nhs 10  (Jena Bioscience)


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    Jena Bioscience dbco nhs 10
    Dbco Nhs 10, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jena Bioscience dibenzylcyclooctyne biotin
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    Image Search Results


    Journal: bioRxiv

    Article Title: Simultaneous global labeling (SiGL) of 5-methylcytosine and 5-hydroxymethylcytosine by DNA alkylation with a synthetic cofactor and engineered methyltransferase

    doi: 10.1101/2022.10.31.513813

    Figure Lengend Snippet:

    Article Snippet: Following inactivation, Dibenzocyclooctyl (DBCO)-PEG4-5/6-TAMRA or Dibenzocyclooctyl (DBCO)-Sulfo-Cy5 (Jena Bioscience, Jena, Germany) was added to a final concentration of 250 μM and then incubated overnight at 37°C.

    Techniques: Modification