datp 14 biotin  (Thermo Fisher)


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    Name:
    Biotin 14 dATP
    Description:
    Biotin 14 dATP is a patented dATP analog with biotin attached at the 6 position of the purine base by a 14 atom linker The long 14 atom spacer arm allows accessibility to streptavidin for sensitive detection of probe target hybrids in in situ and filter based hybridizations Biotin 14 dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP dGTP and dTTP 1 Biotin 14 dATP can be added to the 3 end of DNA using terminal deoxynucleotidyl transferase 2 The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation Performance and Quality Testing Purity is evaluated by reverse phase HPLC analysis
    Catalog Number:
    19524016
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Labeling|Nucleic Acid Labeling & Oligo Synthesis
    Category:
    Oligos Primers Probes Nucleotides
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    Structured Review

    Thermo Fisher datp 14 biotin
    Biotin 14 dATP is a patented dATP analog with biotin attached at the 6 position of the purine base by a 14 atom linker The long 14 atom spacer arm allows accessibility to streptavidin for sensitive detection of probe target hybrids in in situ and filter based hybridizations Biotin 14 dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP dGTP and dTTP 1 Biotin 14 dATP can be added to the 3 end of DNA using terminal deoxynucleotidyl transferase 2 The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation Performance and Quality Testing Purity is evaluated by reverse phase HPLC analysis
    https://www.bioz.com/result/datp 14 biotin/product/Thermo Fisher
    Average 98 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    datp 14 biotin - by Bioz Stars, 2020-08
    98/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
    Article Snippet: .. Probes and Fluorescence In situ Hybridization, measurement of sex chromosomes and distance between signals The PCR products for each satDNA family with more than 50 bp were labeled by nick translation using biotin-14-dATP (Invitrogen) or digoxigenin-11-dUTP (Roche, Mannheim, Germany). .. SatDNAs with less than 50 bp were labeled directly at the 5′ end with biotin-14 dATP (Sigma-Aldrich, St Louis, MO, USA) during their synthesis.

    Nick Translation:

    Article Title: Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don
    Article Snippet: .. Plasmid DNA was labelled by nick translation using biotin-14-dATP of the Bionick™ Labeling System (Invitrogen Life Technologies, Paisley, UK). .. Plasmid DNA (0.5–1 μg) was labelled using 50 μL of labelling reaction (using 5 μL of 10× enzyme mix) for 2 h at 16 °C, and the reaction was stopped with 5 μL of 500 mM EDTA (pH 8.0).

    Article Title: Presence and integration of HBV DNA in mouse oocytes
    Article Snippet: .. The 3.2 kb HBV DNA probe was labeled with biotin-14-dATP by nick translation (BioNick DNA Labeling System, GIBCO BRL). .. Unincorporated nucleotides were separated by cold ethanol precipitation method.

    Article Title: First record of supernumerary (B) chromosomes in electric fish (Gymnotiformes) and the karyotype structure of three species of the same order from the upper Paran? River basin
    Article Snippet: .. The probes were labelled with biotin 14-dATP via nick translation (Bio Nick Labeling System - Gibco, BRL). .. We incubated the slides with RNase (37 ºC, 1 h) and then treated them with 30 μl of hybridization mixture containing 100–300 ng of labelled probes (3 μl), 15 μl 100% formamide, 20×SSC (0.5 μl), 0.1 μl calf thymus DNA, 0.6 μl 50% Dextran and 0.1 μl 10% SDS.

    Article Title: Description of karyotype in Hypostomus regani (Ihering, 1905) (Teleostei, oricariidae) from the Piumhi river in Brazil with comments on karyotype variation found in Hypostomus
    Article Snippet: .. Both probes were labeled with 14-dATP biotin by nick translation, following the manufacturer’s instructions (Bionick Labeling System – Invitrogen). .. Amplification and detection of the hybridization signals was carried out using the avidin-FITC and anti-avidin biotin complex system (Sigma).

    Article Title: Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
    Article Snippet: .. Probes and Fluorescence In situ Hybridization, measurement of sex chromosomes and distance between signals The PCR products for each satDNA family with more than 50 bp were labeled by nick translation using biotin-14-dATP (Invitrogen) or digoxigenin-11-dUTP (Roche, Mannheim, Germany). .. SatDNAs with less than 50 bp were labeled directly at the 5′ end with biotin-14 dATP (Sigma-Aldrich, St Louis, MO, USA) during their synthesis.

    Labeling:

    Article Title: Tracking the evolution of sex chromosome systems in Melanoplinae grasshoppers through chromosomal mapping of repetitive DNA sequences
    Article Snippet: .. Probes labeled with digoxigenin-11-dUTP were detected using anti-digoxigenin-rhodamine (Roche), and probes labeled with biotin-14-dATP were detected using streptavidin, alexa fluor 488 conjugate (Invitrogen). .. The preparations were counterstained using 4′, 6-diamidine-2′-phenylindole dihydrochloride (DAPI) and mounted using Vectashield (Vector, Burlingame, CA, USA).

    Article Title: Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don
    Article Snippet: .. Plasmid DNA was labelled by nick translation using biotin-14-dATP of the Bionick™ Labeling System (Invitrogen Life Technologies, Paisley, UK). .. Plasmid DNA (0.5–1 μg) was labelled using 50 μL of labelling reaction (using 5 μL of 10× enzyme mix) for 2 h at 16 °C, and the reaction was stopped with 5 μL of 500 mM EDTA (pH 8.0).

    Article Title: Presence and integration of HBV DNA in mouse oocytes
    Article Snippet: .. The 3.2 kb HBV DNA probe was labeled with biotin-14-dATP by nick translation (BioNick DNA Labeling System, GIBCO BRL). .. Unincorporated nucleotides were separated by cold ethanol precipitation method.

    Article Title: First record of supernumerary (B) chromosomes in electric fish (Gymnotiformes) and the karyotype structure of three species of the same order from the upper Paran? River basin
    Article Snippet: .. The probes were labelled with biotin 14-dATP via nick translation (Bio Nick Labeling System - Gibco, BRL). .. We incubated the slides with RNase (37 ºC, 1 h) and then treated them with 30 μl of hybridization mixture containing 100–300 ng of labelled probes (3 μl), 15 μl 100% formamide, 20×SSC (0.5 μl), 0.1 μl calf thymus DNA, 0.6 μl 50% Dextran and 0.1 μl 10% SDS.

    Article Title: Description of karyotype in Hypostomus regani (Ihering, 1905) (Teleostei, oricariidae) from the Piumhi river in Brazil with comments on karyotype variation found in Hypostomus
    Article Snippet: .. Both probes were labeled with 14-dATP biotin by nick translation, following the manufacturer’s instructions (Bionick Labeling System – Invitrogen). .. Amplification and detection of the hybridization signals was carried out using the avidin-FITC and anti-avidin biotin complex system (Sigma).

    Article Title: Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
    Article Snippet: .. Probes and Fluorescence In situ Hybridization, measurement of sex chromosomes and distance between signals The PCR products for each satDNA family with more than 50 bp were labeled by nick translation using biotin-14-dATP (Invitrogen) or digoxigenin-11-dUTP (Roche, Mannheim, Germany). .. SatDNAs with less than 50 bp were labeled directly at the 5′ end with biotin-14 dATP (Sigma-Aldrich, St Louis, MO, USA) during their synthesis.

    Article Title: CDK inhibitor p21 is prosurvival in adriamycin-induced podocyte injury, in vitro and in vivo
    Article Snippet: .. Following incubation in One-Phor-All buffer (GE Healthcare, Piscataway, NJ), fragmented DNA was labeled by exposure of sections to diluted TdT (GE Healthcare) and biotin-14-dATP (Invitrogen, Grand Island, NY) for 60 min. ..

    Incubation:

    Article Title: CDK inhibitor p21 is prosurvival in adriamycin-induced podocyte injury, in vitro and in vivo
    Article Snippet: .. Following incubation in One-Phor-All buffer (GE Healthcare, Piscataway, NJ), fragmented DNA was labeled by exposure of sections to diluted TdT (GE Healthcare) and biotin-14-dATP (Invitrogen, Grand Island, NY) for 60 min. ..

    DNA Labeling:

    Article Title: Presence and integration of HBV DNA in mouse oocytes
    Article Snippet: .. The 3.2 kb HBV DNA probe was labeled with biotin-14-dATP by nick translation (BioNick DNA Labeling System, GIBCO BRL). .. Unincorporated nucleotides were separated by cold ethanol precipitation method.

    Polymerase Chain Reaction:

    Article Title: Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
    Article Snippet: .. Probes and Fluorescence In situ Hybridization, measurement of sex chromosomes and distance between signals The PCR products for each satDNA family with more than 50 bp were labeled by nick translation using biotin-14-dATP (Invitrogen) or digoxigenin-11-dUTP (Roche, Mannheim, Germany). .. SatDNAs with less than 50 bp were labeled directly at the 5′ end with biotin-14 dATP (Sigma-Aldrich, St Louis, MO, USA) during their synthesis.

    In Situ Hybridization:

    Article Title: Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
    Article Snippet: .. Probes and Fluorescence In situ Hybridization, measurement of sex chromosomes and distance between signals The PCR products for each satDNA family with more than 50 bp were labeled by nick translation using biotin-14-dATP (Invitrogen) or digoxigenin-11-dUTP (Roche, Mannheim, Germany). .. SatDNAs with less than 50 bp were labeled directly at the 5′ end with biotin-14 dATP (Sigma-Aldrich, St Louis, MO, USA) during their synthesis.

    Plasmid Preparation:

    Article Title: Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don
    Article Snippet: .. Plasmid DNA was labelled by nick translation using biotin-14-dATP of the Bionick™ Labeling System (Invitrogen Life Technologies, Paisley, UK). .. Plasmid DNA (0.5–1 μg) was labelled using 50 μL of labelling reaction (using 5 μL of 10× enzyme mix) for 2 h at 16 °C, and the reaction was stopped with 5 μL of 500 mM EDTA (pH 8.0).

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  • 98
    Thermo Fisher biotin 14 datp
    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, <t>biotin-14-dCTP,</t> and <t>biotin-14-dATP,</t> are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.
    Biotin 14 Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin 14 datp/product/Thermo Fisher
    Average 98 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    biotin 14 datp - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    Image Search Results


    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    doi: 10.3389/fbioe.2019.00401

    Figure Lengend Snippet: Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Article Snippet: Biotin-14-dCTP and biotin-14-dATP were obtained from Thermo Scientific Co., Ltd. (Shanghai, China).

    Techniques: Labeling, Amplification

    Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    doi: 10.3389/fbioe.2019.00401

    Figure Lengend Snippet: Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Article Snippet: Biotin-14-dCTP and biotin-14-dATP were obtained from Thermo Scientific Co., Ltd. (Shanghai, China).

    Techniques: