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RT-qPCR validation of RNA-sequencing. Top 6 downregulated (lower expression in blood-exposed samples) and top 6 upregulated (higher expression in blood-exposed samples). All statistical analyses were performed with unpaired t-tests. ns = not significant, ns = not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, N = 3, α = 0.05.

Journal: bioRxiv

Article Title: Adult organotypic brain slice cultures recapitulate extracellular matrix remodelling in haemorrhagic stroke

doi: 10.1101/2025.07.15.665021

Figure Lengend Snippet: RT-qPCR validation of RNA-sequencing. Top 6 downregulated (lower expression in blood-exposed samples) and top 6 upregulated (higher expression in blood-exposed samples). All statistical analyses were performed with unpaired t-tests. ns = not significant, ns = not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, N = 3, α = 0.05.

Article Snippet: All visualisation of RNA- sequencing data was performed using GraphPad Prism 10.3.

Techniques: Quantitative RT-PCR, Biomarker Discovery, RNA Sequencing, Expressing