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Vector TrueVIEW Autofluorescence Quenching Kit with DAPI

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The Vector TrueVIEW Autofluorescence Quenching Kit with DAPI provides a novel way to diminish unwanted autofluorescence from non lipofuscin sources and dramatically improve signal to noise ratio It removes unwanted autofluorescence in tissue sections due to aldehyde fixation red blood cells and structural elements such as collagen and elastin The quenching action of the kit reagents provides a clear unambiguous “true view localization of the target antigen

Catalog Number:

SP-8500

Price:

None

Category:

Protein chemifluorescent detection reagents or kits or substrates

Size:

15 ml

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Structured Review

Vector Laboratories dapi

Vector TrueVIEW Autofluorescence Quenching Kit with DAPI

dapi - by Bioz Stars, 2021-04

86/100 stars

Images

1) Product Images from "Characterization and isolation of highly purified porcine satellite cells"

Article Title: Characterization and isolation of highly purified porcine satellite cells

Journal: Cell Death Discovery

doi: 10.1038/cddiscovery.2017.3

Pig satellite cells participated in the muscle regeneration in recipient mice and lose the engraftment efficiency after long-term expansion in vitro . ( a ) Four weeks after transplantation, engraftment efficiency of freshly isolated pig satellite cells was determined by Dystrophin and Lamin A/C immunofluorescent staining with muscle sections derived from receipient mice. PBS was injected as negative control. Blue indicated DAPI; red indicated Lamin A/C; green indicated Dystrophin. Scale bars, 20 μ m. ( b ) Lamin A/C, Myh3 immunofluorescent staining with series muscle sections derived from recipient mice 7 days after transplantation. Blue indicated DAPI; red indicated Lamin A/C ( b , left) or Myh3 ( b , right); green indicated Dystrophin. Scale bars, 100 μ m. ( c ) Representative immunofluorescent staining images of Dystrophin and Lamin A/C on mouse sections from mice injected with freshly isolated ( c , left), P2 ( c , middle), and P5 ( c , right) satellite cells. Blue indicated DAPI; red indicated Lamin A/C; green indicated Dystrophin. Scale bars, 100 μ m. ( d ) Quantification of the number of Lamin A/C + fibers in each TA 4 weeks after transplantation. Error bars represented S.E.M. and were based on four independent experiments. Significance was analyzed by student’s t -test. * indicated P

Figure Legend Snippet: Pig satellite cells participated in the muscle regeneration in recipient mice and lose the engraftment efficiency after long-term expansion in vitro . ( a ) Four weeks after transplantation, engraftment efficiency of freshly isolated pig satellite cells was determined by Dystrophin and Lamin A/C immunofluorescent staining with muscle sections derived from receipient mice. PBS was injected as negative control. Blue indicated DAPI; red indicated Lamin A/C; green indicated Dystrophin. Scale bars, 20 μ m. ( b ) Lamin A/C, Myh3 immunofluorescent staining with series muscle sections derived from recipient mice 7 days after transplantation. Blue indicated DAPI; red indicated Lamin A/C ( b , left) or Myh3 ( b , right); green indicated Dystrophin. Scale bars, 100 μ m. ( c ) Representative immunofluorescent staining images of Dystrophin and Lamin A/C on mouse sections from mice injected with freshly isolated ( c , left), P2 ( c , middle), and P5 ( c , right) satellite cells. Blue indicated DAPI; red indicated Lamin A/C; green indicated Dystrophin. Scale bars, 100 μ m. ( d ) Quantification of the number of Lamin A/C + fibers in each TA 4 weeks after transplantation. Error bars represented S.E.M. and were based on four independent experiments. Significance was analyzed by student’s t -test. * indicated P

Techniques Used: Mouse Assay, In Vitro, Transplantation Assay, Isolation, Staining, Derivative Assay, Injection, Negative Control

2) Product Images from "Nuclear removal during terminal lens fiber cell differentiation requires CDK1 activity: appropriating mitosis-related nuclear disassembly"

Article Title: Nuclear removal during terminal lens fiber cell differentiation requires CDK1 activity: appropriating mitosis-related nuclear disassembly

Journal: Development (Cambridge, England)

doi: 10.1242/dev.106005

CDK1 deficiency decreased the phosphorylation of lamin A/C, blocked the entry of DLAD into the nucleus and decreased DNA degradation in maturing lens fiber cells. (A-H,J,K) Primary antibodies to p57 KIP2 (A,D), p27 KIP1 (B,E), pLamin A/C (C,F) and DLAD (H,K) were used on E17.5 Cdk1 L/L (A,B,C,G,H) and MLR10; Cdk1 L/L (D,E,F,J,K) lens sections to detect appropriate antigens. (I,L) TUNEL analysis on E17.5 Cdk1 L/L (I) and MLR10; Cdk1 L/L (L) lens sections revealed DNA degradation. (A,D) p57 KIP2 expression (green stained nuclei) initiated in transitional zone epithelial cells in both control ( Cdk1 L/L ) and Cdk1 -deficient ( MLR10; Cdk1 L/L ) lenses but declined quickly as fiber cells elongated (bracket). (B,E) By contrast, p27 KIP1 persisted in the nuclei of fiber cells deep into the cortex of the control lens (bracket in B) but remained more cortical in the MLR10; Cdk1 L/L lenses (E). (C) Lamin A/C phosphorylation (green foci, dashed circle) initiated near the center of the lens where the organelle-free zone is forming. (H,I) In the same region where pLamin A/C is detected in the control lenses, both DLAD-positive nuclei (lower right inset, H) and TUNEL-positive foci (I, yellow staining, arrows) are found. (F,K,L) MLR10; Cdk1 L/L lenses do not contain pLamin A/C (F), display reduced TUNEL-positive fiber cell foci (L, yellow staining, arrow) and exhibit DLAD accumulation around rather than within late fiber cell nuclei (lower right inset, K). Upper right insets in H and K are high magnifications of cortical fiber cells where DLAD expression is comparatively weak; lower right insets are high magnifications of more mature fiber cells where DLAD expression is clearly evident. Nuclei are counterstained with DAPI, which is blue in A-G,J but pseudocolored red in I and L to enhance the contrast for the TUNEL assay. Scale bars: 100 µm in A-F; 200 µm in G-L; 20 µm in H,K (insets).

Figure Legend Snippet: CDK1 deficiency decreased the phosphorylation of lamin A/C, blocked the entry of DLAD into the nucleus and decreased DNA degradation in maturing lens fiber cells. (A-H,J,K) Primary antibodies to p57 KIP2 (A,D), p27 KIP1 (B,E), pLamin A/C (C,F) and DLAD (H,K) were used on E17.5 Cdk1 L/L (A,B,C,G,H) and MLR10; Cdk1 L/L (D,E,F,J,K) lens sections to detect appropriate antigens. (I,L) TUNEL analysis on E17.5 Cdk1 L/L (I) and MLR10; Cdk1 L/L (L) lens sections revealed DNA degradation. (A,D) p57 KIP2 expression (green stained nuclei) initiated in transitional zone epithelial cells in both control ( Cdk1 L/L ) and Cdk1 -deficient ( MLR10; Cdk1 L/L ) lenses but declined quickly as fiber cells elongated (bracket). (B,E) By contrast, p27 KIP1 persisted in the nuclei of fiber cells deep into the cortex of the control lens (bracket in B) but remained more cortical in the MLR10; Cdk1 L/L lenses (E). (C) Lamin A/C phosphorylation (green foci, dashed circle) initiated near the center of the lens where the organelle-free zone is forming. (H,I) In the same region where pLamin A/C is detected in the control lenses, both DLAD-positive nuclei (lower right inset, H) and TUNEL-positive foci (I, yellow staining, arrows) are found. (F,K,L) MLR10; Cdk1 L/L lenses do not contain pLamin A/C (F), display reduced TUNEL-positive fiber cell foci (L, yellow staining, arrow) and exhibit DLAD accumulation around rather than within late fiber cell nuclei (lower right inset, K). Upper right insets in H and K are high magnifications of cortical fiber cells where DLAD expression is comparatively weak; lower right insets are high magnifications of more mature fiber cells where DLAD expression is clearly evident. Nuclei are counterstained with DAPI, which is blue in A-G,J but pseudocolored red in I and L to enhance the contrast for the TUNEL assay. Scale bars: 100 µm in A-F; 200 µm in G-L; 20 µm in H,K (insets).

Techniques Used: TUNEL Assay, Expressing, Staining

$MLR10; Cdk1 L/L lens epithelial cells continue to synthesize DNA but fail to enter mitosis. (A-H) BrdU incorporation and phosphorylated histone H3 (pHH3) immunohistochemistry (green nuclei) were used to determine the proportion of cells in S phase (A-D) and late G2 phase (E-H), respectively. Nuclei were counterstained with DAPI. The proliferation index (S-phase fraction) did not differ significantly between MLR10; Cdk1 L/L and Cdk1 L/L lenses at E12.5 or at E15.5 (compare B with A, solid bars in I) but significantly increased in MLR10; Cdk1 L/L lenses by E17.5 (compare D with C, solid bars in I). Although there were fewer overall BrdU-positive nuclei in the MLR10; Cdk1 L/L lenses, the proportion of total nuclei that were BrdU positive was relatively increased at E17.5. The proportion of pHH3-positive cells levels were significantly higher in Cdk1 -deficient lenses beginning at E15.5 (compare E with F, lightly shaded bars in I) and most remaining epithelial cells in MLR10; Cdk1 L/L lenses stained positive for pHH3 by E17.5 (compare H with G, lightly shaded bars in I). (J,K) Whole lenses from Cdk1 L/L (J) and MLR10; Cdk1 L/L mice (K) were stained with DAPI, and the intact epithelium was visualized by confocal microscopy to visualize the size and density of epithelial nuclei. (K,L) MLR10; Cdk1 L/L lenses exhibited a significant increase in nuclear size and an increased DAPI staining foci in each cell. All bars represent the mean ± s.e.m., with each bar representing nine measurements (three sections from each of three different embryos). Scale bars: 100 µm in A-D; 200 µm in E-H; 20 µm J,K. le, lens; re, retina; epi, lens epithelium.$
Figure Legend Snippet: MLR10; Cdk1 L/L lens epithelial cells continue to synthesize DNA but fail to enter mitosis. (A-H) BrdU incorporation and phosphorylated histone H3 (pHH3) immunohistochemistry (green nuclei) were used to determine the proportion of cells in S phase (A-D) and late G2 phase (E-H), respectively. Nuclei were counterstained with DAPI. The proliferation index (S-phase fraction) did not differ significantly between MLR10; Cdk1 L/L and Cdk1 L/L lenses at E12.5 or at E15.5 (compare B with A, solid bars in I) but significantly increased in MLR10; Cdk1 L/L lenses by E17.5 (compare D with C, solid bars in I). Although there were fewer overall BrdU-positive nuclei in the MLR10; Cdk1 L/L lenses, the proportion of total nuclei that were BrdU positive was relatively increased at E17.5. The proportion of pHH3-positive cells levels were significantly higher in Cdk1 -deficient lenses beginning at E15.5 (compare E with F, lightly shaded bars in I) and most remaining epithelial cells in MLR10; Cdk1 L/L lenses stained positive for pHH3 by E17.5 (compare H with G, lightly shaded bars in I). (J,K) Whole lenses from Cdk1 L/L (J) and MLR10; Cdk1 L/L mice (K) were stained with DAPI, and the intact epithelium was visualized by confocal microscopy to visualize the size and density of epithelial nuclei. (K,L) MLR10; Cdk1 L/L lenses exhibited a significant increase in nuclear size and an increased DAPI staining foci in each cell. All bars represent the mean ± s.e.m., with each bar representing nine measurements (three sections from each of three different embryos). Scale bars: 100 µm in A-D; 200 µm in E-H; 20 µm J,K. le, lens; re, retina; epi, lens epithelium.

Techniques Used: BrdU Incorporation Assay, Immunohistochemistry, Staining, Mouse Assay, Confocal Microscopy

MLR10; Cdk1 L/L lenses remove both mitochondria and endoplasmic reticulum, despite retaining nuclei. Mitochondria were detected by Tom20 (A,B,D,E) and endoplasmic reticulum by PDI (C,F) immunofluorescence (green staining) in E17.5 lenses. Cdk1 L/L (A-C) and MLR10; Cdk1 L/L (D-F) lens fiber cells lose their mitochondria (A,B,D,E) and endoplasmic reticulum (C,F) prior to reaching the center of the lens. Tom20 staining drops precipitously outside the most peripheral fiber cells (asterisks in A,D) but remains as punctate foci until the deep fiber cells of the central zone (arrows in A,D). Cdk1 L/L lenses form an organelle-free zone (OFZ) lacking both mitochondria and nuclei (B). The MLR10; Cdk1 L/L central lens fibers lack mitochondria, but retain nuclei (E, yellow arrowheads). Likewise, both control (C) and MLR10; Cdk1 L/L (F) lenses remove PDI-staining endoplasmic reticulum from mature nuclear fiber cells (lack of green staining within the dotted line border in C,F). Nuclei were counterstained with DAPI (B,E). Scale bars: 100 µm A,B,D,E; 200 µm C,F. epi, lens epithelium; cf, central fiber cells.

Figure Legend Snippet: MLR10; Cdk1 L/L lenses remove both mitochondria and endoplasmic reticulum, despite retaining nuclei. Mitochondria were detected by Tom20 (A,B,D,E) and endoplasmic reticulum by PDI (C,F) immunofluorescence (green staining) in E17.5 lenses. Cdk1 L/L (A-C) and MLR10; Cdk1 L/L (D-F) lens fiber cells lose their mitochondria (A,B,D,E) and endoplasmic reticulum (C,F) prior to reaching the center of the lens. Tom20 staining drops precipitously outside the most peripheral fiber cells (asterisks in A,D) but remains as punctate foci until the deep fiber cells of the central zone (arrows in A,D). Cdk1 L/L lenses form an organelle-free zone (OFZ) lacking both mitochondria and nuclei (B). The MLR10; Cdk1 L/L central lens fibers lack mitochondria, but retain nuclei (E, yellow arrowheads). Likewise, both control (C) and MLR10; Cdk1 L/L (F) lenses remove PDI-staining endoplasmic reticulum from mature nuclear fiber cells (lack of green staining within the dotted line border in C,F). Nuclei were counterstained with DAPI (B,E). Scale bars: 100 µm A,B,D,E; 200 µm C,F. epi, lens epithelium; cf, central fiber cells.

Techniques Used: Immunofluorescence, Staining

Staining:

Article Title: Role of epithelial integrin-linked kinase in promoting intestinal inflammation: effects on CCL2, fibronectin and the T cell repertoire
Article Snippet: For detection of Th17 cells by immunofluorescence, the slides were processed as for IHC and the following antibodies were used: DAPI and IL-17A (eBio 17B7). .. Sections were stained with Vectastain ABC elite kit and biotinylated ant-rabbit for DAPI, or eFluor650 Nanocrystal conjugation kit, cat no. 88-7072-98 antibody, and Avidin D-FITC (or Avidin-Texas Red) used for immunofluorescence (Vector laboratories, CA, USA). ..

Article Title: Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1
Article Snippet: After a 2-hour pulse, the hearts were collected and processed for frozen sections as described above in Immunostaining Section. .. The serial sections crossing the whole heart were first stained with PECAM1 or VEGFR3 antibody followed by EdU staining with EdU imaging Kit (Life Technology) and counterstained with DAPI (Vector lab). .. The stained sections were photographed using a Zeiss Observer Z1 or Leica SP5 confocal microscope.

Article Title: Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter rodentium-induced colitis
Article Snippet: For detection of Tir and LPS by immunofluorescence, the slides were processed as for IHC and the following antibodies were used: Tir and LPS (Vallance). .. Sections were stained with Vectastain ABC elite kit and biotinylated ant-rabbit for DAPI, or eFluor650 Nanocrystal conjugation kit, cat no. 88-7072-98 antibody, and Avidin D-FITC (or Avidin-Texas Red) used for immunofluorescence (Vector laboratories, CA, USA). .. A Zeiss LSM-780 microscope was used to capture images.

Article Title: The EJC component Magoh regulates proliferation and expansion of neural crest-derived melanocytes
Article Snippet: The following antibodies were used at 1:200: mouse anti- -galactosidase (Promega, madison, WI), rabbit anti-cleaved Caspase-3 (Cell Signaling, Danvers, MA), rabbit anti-phospho-Histone H3 (Upstate Biotechnology, Temecula, CA), and anti-mouse and anti-rabbit FITC-conjugated and rhodamine-conjugated secondary antibodies (Invitrogen, CA). .. Staining was performed using the MOM kit and Vectashield mount with DAPI (Vector labs). ..

Article Title: Nanoparticle engineered TRAIL-overexpressing adipose-derived stem cells target and eradicate glioblastoma via intracranial delivery
Article Snippet: Another seven GBM orthotopic mice underwent repetitive injection of NPs/pT RAIL-hADSCs at day 5 and day 15 after inoculation. .. To detect in vivo apoptotic activity, tumor-bearing brain sections were stained with a TUNEL assay kit (Roche) and mounted with Vectashield mounting medium with DAPI (Vector Lab). .. Imaging was performed with the fluorescence microscope (Carl Zeiss).

Article Title: Preclinical Evaluation of Intravesical Cisplatin Nanoparticles for Non–Muscle-Invasive Bladder Cancer
Article Snippet: .. Immunofluorescence was performed on a separate set of slides which were stained for TUNEL per manufacturer instructions (ApopTag Peroxidase In Situ Apoptosis Detection Kit, Millipore) and counterstained with DAPI (Vector Laboratories). ..

Article Title: Platelet-Derived Growth Factor BB Induces Nuclear Export and Proteasomal Degradation of CREB via Phosphatidylinositol 3-Kinase/Akt Signaling in Pulmonary Artery Smooth Muscle Cells
Article Snippet: SuperSignal West Pico chemiluminescent substrate and NE-PER nuclear and cytoplasmic extract reagents were from Pierce (Rockford, IL). .. Vector NovaRed peroxidase staining kits, VectaShield mounting medium with DAPI (4′,6′-diamidino-2-phenylindole), and peroxidase-conjugated goat anti-rabbit secondary antibody were from Vector Laboratories (Burlingame, CA). .. The plasmid pECFP-C1 was purchased from Clontech Laboratories, Inc. (Palo Alto, CA).

Conjugation Assay:

Avidin-Biotin Assay:

Immunofluorescence:

Microscopy:

Article Title: Targeting β-tubulin:CCT-β complexes incurs Hsp90- and VCP-related protein degradation and induces ER stress-associated apoptosis by triggering capacitative Ca2+ entry, mitochondrial perturbation and caspase overactivation
Article Snippet: For mitochondrial staining, cells were incubated with MitoTracker (Invitrogen) for another 15 min at RT. .. After mounting the cells with a commercial kit containing DAPI reagent (Vector Laboratories), the cells were analyzed using a FluoView confocal microscope system (Olympus). .. Determination of caspase activity The caspase activity assay was performed according to the manufacturer's guidelines (BioVision).