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Vector Laboratories dapi vector laboratories cat
Dapi Vector Laboratories Cat, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+vector+laboratories+cat/VECTASHIELD+Vibrance+Antifade+Mounting+Medium+with+DAPI/pm41198902-260-60-61
Average 96 stars, based on 712 article reviews

dapi vector laboratories cat - by Bioz Stars, 2026-09

96/100 stars

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Article Title: Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity
Article Snippet: DOI: https://doi.org/10.7554/eLife.47498 20 of 33 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Mitochondrial Membrane Potential Assay kit Cell Signaling Cat. #: 13296 final concentration of TMRE dye: 200 nM Chemical compound, drug protease/phosphatase inhibitors Pierce Cat. #: 88669 Chemical compound, drug RapiGest Waters Cat. #: 186002123 Chemical compound, drug DTT Thermo Fisher Scientific Cat. #: 20290 Chemical compound, drug chloroacetamide Sigma Cat. #: 22790 Chemical compound, drug L-alanyl-Lglutamine Millipore Cat. #: K0302 Chemical compound, drug MEM-Vitamine Sigma Cat. #: K0373 Chemical compound, drug Mito+Serum extender Corning Costar Cat. #: 355006 Chemical compound, drug FUDR Sigma Cat. #: F0503 Chemical compound, drug Thioflavin S Santa Cruz Cat. #: CAS 1326-12-1 Chemical compound, drug hemin Sigma Cat. #: 51289 final concentration: 10 mM Chemical compound, drug tert-butylhydroperoxide Sigma Cat. #: 458139 final concentration: 25 mM Chemical compound, drug 1 mM staurosporine Santa Cruz Cat. #: sc-3510 final concentration: 1 mM Software, algorithm MaxQuant, software package version 1.5.0.25 (Cox and Mann, 2008) RRID:SCR_014485 Software, algorithm Andromeda search engine (Cox et al., 2011) Software, algorithm Perseus, version 1.5.5.3 (Cox and Mann, 2008) RRID:SCR_015753 Software, algorithm Ingenuity Pathway Analysis QIAGEN Inc RRID:SCR_008653 Software, algorithm GraphPad Prism GraphPad Prism (https://graphpad.com) RRID:SCR_015807 Other Vectashield mounting medium containing DAPI Vector Laboratories Cat. #: VEC-H-1500 RRID:AB_2336788

Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stellar Competent Cells Takara Bio Cat #636763 Ready-to-use AAV9 Virus (100 mL at titer R131013 vg/mL Addgene Cat #105537-AAV9 Chemicals, peptides, and recombinant proteins Hy-Clone DMEM high glucose Cytiva Cat #SH30022.01 Gibco DMEM, High Glucose Gibco Cat #11965118 Ham’s F10, with L-Glutamine Cytiva Cat #SH30025.01 Ham’s F-10 nutrient mixture Gibco Cat #11550043 USDA Origin FBS Peak Serum Cat #PS-FB2 FBS – USDA Origin Pure Premium, Tet-Free Peak Serum Cat # PS-FB3 Horse Serum, heat inactivated, New Zealand origin Gibco Cat # 26050088 Sodium Pyruvate (100 mM) Gibco Cat #11360070 Penicillin-Streptomycin (5,000 U/mL) Gibco Cat #15070063 L Glutamine (200mM) Gibco Cat #25030081 Doxycycline Hydrochloride, Ready Made Solution Sigma Aldrich Cat #D3072 Recombinant Human FGF-basic PeproTech Cat #100-18C Matrigel Basement Membrane Matrix Corning Cat #354234 Puromycin Dihydrochloride Gibco Cat #A1113802 Blasticidin S HCl Gibco Cat #A1113903 FuGENE-6 Promega Cat #E2692 Polyethylenimine (PEI) Polysciences Cat #24314-2 Collagenase, Type 2 Worthington Biochemical Cat # LS004176 Pronase, from Streptomyces griseus Sigma Aldrich Cat #10165921001 HEPES (1 M) Gibco Cat #15630106 Vybrant Multicolor Cell-Labeling Kit (DiO, DiI, DiD Solutions, 1 mL each) Invitrogen Cat #V22889 DPBS without Calcium and Magnesium Gibco Cat #14190250 Ultrapure DNase RNase free water Invitrogen Cat #10977023 Acetone Sigma Aldrich Cat # 320110 Paraformaldehyde, 16% w/v aq. soln., methanol free Thermo Scientific Cat # 43368 Cardiotoxin EMD Millipore Cat #217503-1MG Evans blue dye Sigma Aldrich Cat #E2129-10G D-luciferin Promega Cat #E1605 D-luciferin Goldbio Cat #LUCK-2G Hoechst 33342 Invitrogen Cat #H3570 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat #H1200 Ammonium molybdate tetrahydrate Sigma Aldrich Cat #A7302-100G Phosphotungstic Acid (PTA) Electron Microscopy Sciences Cat #19500 Masson Trichrome Kit Richard-Allan Scientific Cat # 22-110-648 Halt Protease Inhibitor Cocktail, EDTA-Free (100X) Thermo Scientific Cat #78437 Phosphatase Inhibitor Cocktail 2 Sigma Aldrich Cat #P5726 Bio-Rad Protein Assay Dye Reagent Concentrate BioRad Cat #5000006 Critical commercial assays InFusion HD cloning plus Kit Clontech Cat #638909 Experimental models: Cell lines Mouse primary myoblasts from C57BL/6J mice This study N/A C3H/10T1/2 fibroblasts ATCC Cat #CCL-226; RRID:CVCL_0190 (Continued on next page) ll Cell 186, 2062–2077.e1–e9, May 11, 2023 e2 Article

Recombinant:

Article Title: Bach2 repression of CD36 regulates lipid-metabolism-linked effector functions in follicular B cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse B220-FITC BD Biosciences Cat# 553088; RRID:AB_394618 Anti-mouse CD36-PE BD Biosciences Cat# 562702; RRID:AB_2737732 Anti-mouse CD138-PE BD Biosciences Cat# 553714; RRID:AB_395000 Anti-mouse CD19-PerCP BD Biosciences Cat#551001; RRID:AB_394004 Anti-mouse CD19-APC BD Biosciences Cat#550992; RRID:AB_398483 Anti-mouse phospho-p38-FITC BD Biosciences Cat#612594; RRID:AB_399877 Anti-mouse CD43-PEcy7 BD Biosciences Cat#562866; RRID:AB_2737852 Anti-mouse CD23-PEcy7 BD Biosciences Cat#562825; RRID:AB_2737820 Anti-mouse CD21-FITC Biolegend Cat#123408; RRID:AB_940405 Anti-mouse IL-6-FITC Invitrogen Cat#11-7061-81; RRID:AB_465394 Anti-mouse Bach2 Rockland Immunochemicals Cat# 600-401-H35; RRID: AB_2614304 Normal Rabbit IgG Cell Signaling Cat#2729; RRID:AB_1031062 Chemicals, peptides, and recombinant proteins Live/Dead Dead Fixable Near-IR Dead Cell kit Invitrogen Cat# L10119 MitoTracker Green MitoTracker Deep Red Invitrogen Cat#M22426 MitoProbeTM TMRM Assay Kit Invitrogen Cat#M20036 MitoSOXTM Mitochondrial Superoxide Indicators Invitrogen Cat#36008 Bodipy 493/503 Invitrogen Cat#D3922 C16-Bodipy Invitrogen Cat#D3821 C11-Bodipy 581/591 Invitrogen Cat#D3861 Dil-oxLDL Invitrogen Cat#L34358 Palmitic acid Sigma-aldrich Cat#P5585 Fatty-acid free BSA Sigma-aldrich Cat#A0281 Lipopolysaccharide Sigma-Aldrich Cat#L8274 Resiquimod Enzo Cat#ALX-420-038-M005 sulfo-N-succinimidyl oleate Cayman Chemical Cat#11211 GW3965 Chemscene Cat# 405911-09-3 SB203580 Selleckchem Cat# S1076 2-deoxy-D-glucose Sigma-Aldrich Cat#D8375 Etomoxir Sigma-Aldrich Cat# 236020 TRIzol reagent Invitrogen Cat#15596026 Vectashield with DAPI Vector Laboratories CatH1500-10 Cytofix/CytopermTM Fixation/Permeabilization Kit BD Biosciences Cat#554714 Perm/Wash Buffer BD Biosciences Cat#554723 Critical commercial assays Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit BioVision Cat#K603-100 Chromatin Immunoprecipitation (ChIP) Assay Kit Merck Millipore Cat#17-295 Secrete Pair Gaussia Luciferase Assay kit GeneCopoeia Cat#LF061 Reverse transcribed into cDNA using AmfiRivert platinum cDNA synthesis master mix GeneDepot Cat#R5600-050 Mouse IL-6 uncoated ELISA Invitrogen Cat#88-7064 Total IgG ELISA KIT Bethyl Laboratories, Inc. Cat#E99-131 (Continued on next page) Cell Reports 43, 114878, November 26, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Total IgM ELISA KIT Bethyl Laboratories, Inc. Cat#E99-101 Experimental models: Organisms/strains Mouse: Bach2 Knock-Out Tohoku University N/A Mouse: CD36 fl/fl Jackson Laboratory Cat#032276 Mouse: CD19-cre Jackson Laboratory Cat#006785 Experimental models: Cell line Jurkat cells ATCC Cat# TIB-152 Oligonucleotides CpG oligonucleotide BD Biosciences Cat#554714 Deoxyribonucleic acid Sigma Cat#D1501 b-actin-FWD: 50-GACGGCCAGGTCATCACTATTG -30 This paper N/A b-actin-REV: 50-AGGAAGGCTGGAAAAGAGCC-30 This paper N/A Bach2-FWD: 50-CTGTAGCCTTCTCATCTCTTCC-30 This paper N/A Bach2-REV: 50-TCCTTCTTCCGTTGGTCATTG-30 This paper N/A Blimp1-FWD: 50-ACCGTCTTGAGGACATGGAG-30 This paper N/A Blimp1-REV: GTTGCTGTGAGGCAACTTCA-30 This paper N/A Cd36-FWD: 50-GCATGGCAGCTTTGGGCAGG-30 This paper N/A Cd36-REV: 50-TGCACAGGAGAGGCGGGCAT-30 This paper N/A Uchl1-FWD: 50-GAAGCAGACCATCGGAAACTCC-30 This paper N/A Uchl1-REV: 50-GGACAGCTT CTCCGTTTCAGAC-30 This paper N/A Lxr-b-FWD: 50-CGCTACAACCACGAGACAGA-30 This paper N/A Lxr-b-REV: 50-TGTTGATGGCGATAAGCAAG-30 This paper N/A Abca1-FWD: 50-CTCAGTTAAGGCTGCTGCTG- 30 This paper N/A Abca1-REV: 50-TCAGGCGTACAGAGATCAGG-3’.

Chromatin Immunoprecipitation:

Luciferase:

Reverse Transcription:

cDNA Synthesis:

Enzyme-linked Immunosorbent Assay:

Labeling:

Article Title: Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional Information Antibody Rabbit polyclonal anti-Dnmt3a Santa Cruz Biotechnology Cat. #: 20703; RRID:AB_2093990 (1:1000) Antibody Rabbit polyclonal anti-MeCP2 Huda Zoghbi lab (in house) 0535 (1:10,000) Antibody Rabbit polyclonal anti-histone H3 Abcam Cat. #: 1791; RRID:AB_302613 (1:20,000) Antibody goat anti-rabbit-HRP BioRad Cat. #: 170–5046; RRID:AB_11125757 (1:10,000) Antibody Mouse monoclonal anti-Dnmt3a Novus Cat. #: NB120-13888; RRID:AB_789607 (1:250) Antibody Rabbit polyclonal anti-Myc Sigma Cat. #: C3956; RRID:AB_439680 (1:200) Antibody Rabbit monoclonal anti-MeCP2 Cell Signaling Technology Cat. #: 3456; RRID:AB_2143849 (1:500) Antibody Chicken polyclonal anti-GFP Abcam Cat. #: 13970; RRID:AB_300798 (1:800) Antibody Goat anti-mouse Alexa Fluor 555 Invitrogen Cat. #: A21127; RRID:AB_141596 (1:1000) Antibody Goat polyclonal anti-rabbit Dylight 488 Bethyl Laboratories Cat. #: A120-201D2; RRID:AB_10634085 (1:750) Antibody Goat anti-chicken Alexa Fluor 488 Invitrogen Cat. #: A11039; RRID:AB_142924 (1:750) Antibody Donkey anti-rabbit Alexa Fluor 568 Thermo Fisher Scientific Cat. #: A10042; RRID:AB_2534017 (1:1000) Antibody Mouse monoclonal anti-NeuN Millipore Cat. #: MAB377; RRID:AB_2298772 (1:300) (each batch needs to be empirically tested after labeling with Alexa Fluor 647) Antibody Rabbit polyclonal anti-GFP 488 Thermo Fisher Scientific Cat. #: A-21311; RRID:AB_221477 (1:1000) Other cOmplete, EDTA-free Protease Inhibitor Cocktail Sigma Cat. #: 5056489001 Other Pierce Universal Nuclease for Cell Lysis Thermo Fisher Scientific Cat. #: 88701 Other Optimal Cutting Temperature medium VWR Cat. #: 25608–930 Other VECTASHIELD HardSet Antifade Mounting Medium without DAPI Vector Laboratories Cat. #: H-1400 Other Cryoseal XYL Fisher Scientific Cat. #: 22-050-262 Other Aqueous Glutaraldehyde EM Grade, 10% 10 ML Electron Microscopy Sciences Cat. #: 16100 Dilute fresh Other Paraformaldehyde Sigma Cat. #: P6148 Make fresh Other X-Gal Gold Biotechnologies Cat. #: X4281C Other RNasin Ribonuclease Inhibitors Promega Cat. #: N261A Other UltraPure BSA (50 mg/mL) Thermo Fisher Scientific Cat. # AM2618 Other DPBS, no calcium, no magnesium Invitrogen Cat. #: 14190144 Continued on next page Lavery et al. eLife 2020;9:e52981. .. DOI: 10.7554/eLife.52981 13 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional Information Other UltraPure DEPCTreated Water (1L) Invitrogen Cat. #: 750023 Commercial assay, kit GE Healthcare Amersham ECL Prime Western Blotting Detection Reagent Fisher Scientific Cat. #: 45010090 Commercial assay, kit APEX Alexa Fluor 647 Antibody Labeling Kit Thermo Fisher Scientific Cat. #: A10475 Commercial assay, kit Single Cell RNA Purification Kit Norgen Biotek Cat. #: 51800 Commercial assay, kit NuGEN Ovation RNA-Seq v2 NuGen protocol p/n 7102, kit p/n 7102–08 Commercial assay, kit Rubicon ThruPlex DNA-Seq Rubicon Genomics protocol: QAM-108–002, kit p/n R400428 Commercial assay, kit EZ DNA Methy lation-Direct Kit Zymo Cat. #: D5021 Other Unmethylated lambda DNA spike-in Promega Cat. #: D1521 Strain, Strain background (Mus musculus) Dnmt3aflox/flox mice Dr. Margaret Goodell, Baylor College of Medicine (Can be purchased from Riken BRC) Cat. #: RBRC03731; RRID:IMSR_RBRC03731 Strain, Strain background (Mus musculus) Slc32a1-Cre+/+ mice Jackson Laboratory Cat. #: 017535; RRID:IMSR_JAX:017535 backcrossed to C57Bl/6J Strain, Strain background (Mus musculus) Mecp2flox/flox and Mecp2flox/y mice MMRRC Cat. #: 011918-UCD; RRID:MMRRC_011918-UCD backcrossed to C57Bl/6J Strain, Strain background (Mus musculus) R26-CAG-LSL-Sun1sfGFP-Myc+/+ mice Dr. M. Margarita Behrens, The Salk Institute for Biological Studies (can be purchased from Jackson Laboratory) Cat. #: 021039; RRID:IMSR_JAX:021039 backcrossed to C57Bl/6J Software, algorithm GraphPad Prism version 6 GraphPad Software www.graphpad.com Other pClamp10 Molecular Devices https://www.moleculardevices.com Software, algorithm Minianalysis 6.0.3 Synaptosoft Inc http://www.synaptosoft. com/MiniAnalysis/ Software, algorithm STAR aligner version 2.5.3a GitHub https://github.com/alexdobin/STAR Software, algorithm FeatureCount v1.5.3 Subread http://subread.sourceforge.net Software, algorithm DESeq2 v1.6.2 Bioconductor https://bioconductor.org/packages/ release/bioc/html/DESeq2.html

Protease Inhibitor:

Lysis:

Electron Microscopy:

RNAscope:

Article Title: Secretin targets interstitial cells of Cajal to regulate intestinal contractions.
Article Snippet: .. Reagent or resource Source Identifier RNAscope Wash Buffer ACDBio PN 310091 TSA buffer ACDBio PN 322809 RNAscopeTM ProbeMm-Sctr ACDBio Cat. No. 508511 RNAscope 3-plex Negative control Probe -Mm ACDBio Cat. No. 320871 RNAscopeTM 3-plex Positive Control ProbeMm ACDBio Cat. No. 320881 co-detection antibody dilutant ACDBio Cat. No. 323160 Opal 570 Akoya Biosciences PN FP1488001KT Vectashield Vibrance Antifade Mounting Medium with DAPI Vector laboratories Cat. No. H-1800 Antibodies Goat polyclonal primary antibody- mSCFR R and D systems Cat. No. AF1356 Donkey anti-goat Alexa Fluor® 488 Jackson ImmunoResearch Laboratories Inc. Cat. No. 705-545- 147 .. Reagent or resource Source Identifier RNAscope Wash Buffer ACDBio PN 310091 TSA buffer ACDBio PN 322809 RNAscope ProbeMm-Sctr ACDBio Cat. No. 508511 RNAscope 3-plex Negative control Probe -Mm ACDBio Cat. No. 320871 RNAscope 3-plex Positive Control ProbeMm ACDBio Cat. No. 320881 co-detection antibody dilutant ACDBio Cat. No. 323160 Opal 570 Akoya Biosciences PN FP1488001KT Vectashield Vibrance Antifade Mounting Medium with DAPI Vector laboratories Cat. No. H-1800 Antibodies Goat polyclonal primary antibody- mSCFR R and D systems Cat. No. AF1356 Donkey anti-goat Alexa Fluor 488 Jackson ImmunoResearch Laboratories Inc. Cat. No. 705-545- 147

Negative Control:

Positive Control:

Western Blot:

Article Title: Prolonged Cell Cycle Arrest in Response to DNA damage in Yeast Requires the Maintenance of DNA Damage Signaling and the Spindle Assembly Checkpoint
Article Snippet: Mouse monoclonal anti-Rad53 AbCam Cat # ab166859; RRID:AB_2801547 Rabbit polyclonal anti-Rad53 AbCam ab104232 Mouse monoclonal anti-myc AbCam Cat# ab16918; RRID:AB_30256 Mouse monoclonal anti-Pgk1 AbCam Cat# ab32, RRID:AB_30359 .. Indole-3-acetic acid Sigma-Aldrich I3750-25G-A Formaldehyde Sigma-Aldrich 47608 VECTASHIELD® Antifade Mounting Medium with DAPI Vector-Laboratories Cat. No. H-1200 Prometheus Protein Biology Products 20-313 OneBlockTM Western-CL Blocking Buffer, For Chemiluminescent Blots Genesee Scientific Cat #: 20-313 ECLTM Prime Western Blotting System Millipore Sigma GERPN2232 ..

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