dapi staining (MedChemExpress)
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Dapi Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining/DAPI/pm42365506-96-13-24
Average 98 stars, based on 182 article reviews
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Incubation:Article Title: Lipoamide Attenuates Hypertensive Myocardial Hypertrophy Through PI3K/Akt-Mediated Nrf2 Signaling Pathway. Article Snippet: Cells were inoculated in Confocal dishes (Biosharp, China) and treated with different drugs. .. 50 μM of reactive oxygen fluorescent dye H2DCFDA (MCE, China) was added to each well, and cells were incubated for 1 h in the incubator and washed with PBS, and the ROS production in each group was observed under a laser confocal microscope after nuclear blocking with Article Title: The landscape of cell regulatory and communication networks in the human dental follicle Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by Article Title: Identification of inflammation-related biomarkers and therapeutic targets for neurogenic bladder fibrosis via multi-omics analysis. Article Snippet: Inflammatory responses play a crucial role in the progression of pediatric neurogenic bladder (NB)-associated fibrosis; however, their specific contributions remain poorly understood.. This study aimed to identify inflammation-related biomarkers for diagnosing NB fibrosis and to explore potential therapeutic drugs.. The NB rat model was established by spinal nerve severing, followed by single-cell and bulk RNA sequencing of bladder tissues. Microscopy:Article Title: Lipoamide Attenuates Hypertensive Myocardial Hypertrophy Through PI3K/Akt-Mediated Nrf2 Signaling Pathway. Article Snippet: Cells were inoculated in Confocal dishes (Biosharp, China) and treated with different drugs. .. 50 μM of reactive oxygen fluorescent dye H2DCFDA (MCE, China) was added to each well, and cells were incubated for 1 h in the incubator and washed with PBS, and the ROS production in each group was observed under a laser confocal microscope after nuclear blocking with Article Title: Low-Dose Trans -Resveratrol Ameliorates Diabetes-Induced Retinal Ganglion Cell Degeneration via TyrRS/c-Jun Pathway Article Snippet: Slides were incubated in diluent primary antibody overnight at 4°C, washed with PBS 3 times, and incubated in diluent containing Alexa Fluor 488/594-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature. .. After Blocking Assay:Article Title: Lipoamide Attenuates Hypertensive Myocardial Hypertrophy Through PI3K/Akt-Mediated Nrf2 Signaling Pathway. Article Snippet: Cells were inoculated in Confocal dishes (Biosharp, China) and treated with different drugs. .. 50 μM of reactive oxygen fluorescent dye H2DCFDA (MCE, China) was added to each well, and cells were incubated for 1 h in the incubator and washed with PBS, and the ROS production in each group was observed under a laser confocal microscope after nuclear blocking with Article Title: The landscape of cell regulatory and communication networks in the human dental follicle Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by Staining:Article Title: Lipoamide Attenuates Hypertensive Myocardial Hypertrophy Through PI3K/Akt-Mediated Nrf2 Signaling Pathway. Article Snippet: Cells were inoculated in Confocal dishes (Biosharp, China) and treated with different drugs. .. 50 μM of reactive oxygen fluorescent dye H2DCFDA (MCE, China) was added to each well, and cells were incubated for 1 h in the incubator and washed with PBS, and the ROS production in each group was observed under a laser confocal microscope after nuclear blocking with Article Title: Imipramine inhibits feline infectious peritonitis virus infection by targeting host cholesterol metabolism via NPC1. Article Snippet: .. The procedure closely followed that of the immunofluorescence assay, with the exception that Article Title: The landscape of cell regulatory and communication networks in the human dental follicle Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by Article Title: Identification of inflammation-related biomarkers and therapeutic targets for neurogenic bladder fibrosis via multi-omics analysis. Article Snippet: Inflammatory responses play a crucial role in the progression of pediatric neurogenic bladder (NB)-associated fibrosis; however, their specific contributions remain poorly understood.. This study aimed to identify inflammation-related biomarkers for diagnosing NB fibrosis and to explore potential therapeutic drugs.. The NB rat model was established by spinal nerve severing, followed by single-cell and bulk RNA sequencing of bladder tissues. Article Title: Low-Dose Trans -Resveratrol Ameliorates Diabetes-Induced Retinal Ganglion Cell Degeneration via TyrRS/c-Jun Pathway Article Snippet: Slides were incubated in diluent primary antibody overnight at 4°C, washed with PBS 3 times, and incubated in diluent containing Alexa Fluor 488/594-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature. .. After Article Title: A proteostasis clock underlies the timing of bacterial dormancy and antibiotic tolerance Article Snippet: .. For Immunofluorescence:Article Title: Imipramine inhibits feline infectious peritonitis virus infection by targeting host cholesterol metabolism via NPC1. Article Snippet: .. The procedure closely followed that of the immunofluorescence assay, with the exception that Fluorescence:Article Title: The landscape of cell regulatory and communication networks in the human dental follicle Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by Article Title: Low-Dose Trans -Resveratrol Ameliorates Diabetes-Induced Retinal Ganglion Cell Degeneration via TyrRS/c-Jun Pathway Article Snippet: Slides were incubated in diluent primary antibody overnight at 4°C, washed with PBS 3 times, and incubated in diluent containing Alexa Fluor 488/594-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature. .. After Construct:Article Title: Engineering a Mechanoactive Fibrous Substrate with Enhanced Efficiency in Regulating Stem Cell Tenodifferentiation. Article Snippet: Electrospun-aligned fibers in ultrathin fineness have previously demonstrated a limited capacity in driving stem cells to differentiate into tendon-like cells.. In view of the tendon’s mechanoactive nature, endowing such aligned fibrous structure with mechanoactivity to exert in situ mechanical stimulus by itself, namely, without any forces externally applied, is likely to potentiate its efficiency of tenogenic induction.. To test this hypothesis, in this study, a shape-memory-capable poly(L-lactide-co-caprolactone) (PLCL) copolymer was electrospun into aligned fibrous form followed by a “stretching-recovery” shape-programming procedure to impart shape memory capability. Concentration Assay:Article Title: A proteostasis clock underlies the timing of bacterial dormancy and antibiotic tolerance Article Snippet: .. For |
