Review



dapi staining  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    MedChemExpress dapi staining
    Dapi Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/DAPI/pm42365506-96-13-24
    Average 98 stars, based on 182 article reviews
    dapi staining - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Lipoamide Attenuates Hypertensive Myocardial Hypertrophy Through PI3K/Akt-Mediated Nrf2 Signaling Pathway.
    Article Snippet: Cells were inoculated in Confocal dishes (Biosharp, China) and treated with different drugs. .. 50 μM of reactive oxygen fluorescent dye H2DCFDA (MCE, China) was added to each well, and cells were incubated for 1 h in the incubator and washed with PBS, and the ROS production in each group was observed under a laser confocal microscope after nuclear blocking with DAPI staining. .. All data were expressed as mean ± standard deviation (SD) and were analyzed by SPSS Version 27 software.

    Article Title: The landscape of cell regulatory and communication networks in the human dental follicle
    Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by DAPI staining for 10 min or directly sealing slides with AntiFade Mounting Medium (HY-K1042, MedChemExpress, United States). .. The fluorescence images were captured with CLSM (Nikon, Japan).

    Article Title: Identification of inflammation-related biomarkers and therapeutic targets for neurogenic bladder fibrosis via multi-omics analysis.
    Article Snippet: Inflammatory responses play a crucial role in the progression of pediatric neurogenic bladder (NB)-associated fibrosis; however, their specific contributions remain poorly understood.. This study aimed to identify inflammation-related biomarkers for diagnosing NB fibrosis and to explore potential therapeutic drugs.. The NB rat model was established by spinal nerve severing, followed by single-cell and bulk RNA sequencing of bladder tissues.

    Microscopy:

    Article Title: Lipoamide Attenuates Hypertensive Myocardial Hypertrophy Through PI3K/Akt-Mediated Nrf2 Signaling Pathway.
    Article Snippet: Cells were inoculated in Confocal dishes (Biosharp, China) and treated with different drugs. .. 50 μM of reactive oxygen fluorescent dye H2DCFDA (MCE, China) was added to each well, and cells were incubated for 1 h in the incubator and washed with PBS, and the ROS production in each group was observed under a laser confocal microscope after nuclear blocking with DAPI staining. .. All data were expressed as mean ± standard deviation (SD) and were analyzed by SPSS Version 27 software.

    Article Title: Low-Dose Trans -Resveratrol Ameliorates Diabetes-Induced Retinal Ganglion Cell Degeneration via TyrRS/c-Jun Pathway
    Article Snippet: Slides were incubated in diluent primary antibody overnight at 4°C, washed with PBS 3 times, and incubated in diluent containing Alexa Fluor 488/594-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature. .. After DAPI staining for 5 minutes and another 3 washes with PBS, the slides were covered in AntiFade Mounting Medium (MCE) and then observed under a fluorescence microscope (Olympus BX53) or a confocal microscope (C2; Nikon, Tokyo, Japan). ..

    Blocking Assay:

    Article Title: Lipoamide Attenuates Hypertensive Myocardial Hypertrophy Through PI3K/Akt-Mediated Nrf2 Signaling Pathway.
    Article Snippet: Cells were inoculated in Confocal dishes (Biosharp, China) and treated with different drugs. .. 50 μM of reactive oxygen fluorescent dye H2DCFDA (MCE, China) was added to each well, and cells were incubated for 1 h in the incubator and washed with PBS, and the ROS production in each group was observed under a laser confocal microscope after nuclear blocking with DAPI staining. .. All data were expressed as mean ± standard deviation (SD) and were analyzed by SPSS Version 27 software.

    Article Title: The landscape of cell regulatory and communication networks in the human dental follicle
    Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by DAPI staining for 10 min or directly sealing slides with AntiFade Mounting Medium (HY-K1042, MedChemExpress, United States). .. The fluorescence images were captured with CLSM (Nikon, Japan).

    Staining:

    Article Title: Lipoamide Attenuates Hypertensive Myocardial Hypertrophy Through PI3K/Akt-Mediated Nrf2 Signaling Pathway.
    Article Snippet: Cells were inoculated in Confocal dishes (Biosharp, China) and treated with different drugs. .. 50 μM of reactive oxygen fluorescent dye H2DCFDA (MCE, China) was added to each well, and cells were incubated for 1 h in the incubator and washed with PBS, and the ROS production in each group was observed under a laser confocal microscope after nuclear blocking with DAPI staining. .. All data were expressed as mean ± standard deviation (SD) and were analyzed by SPSS Version 27 software.

    Article Title: Imipramine inhibits feline infectious peritonitis virus infection by targeting host cholesterol metabolism via NPC1.
    Article Snippet: .. The procedure closely followed that of the immunofluorescence assay, with the exception that DAPI staining was replaced by staining with 0.5 mg/mL Filipin complex (MedChemExpress, USA), which specifically binds to unesterified cholesterol. ..

    Article Title: The landscape of cell regulatory and communication networks in the human dental follicle
    Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by DAPI staining for 10 min or directly sealing slides with AntiFade Mounting Medium (HY-K1042, MedChemExpress, United States). .. The fluorescence images were captured with CLSM (Nikon, Japan).

    Article Title: Identification of inflammation-related biomarkers and therapeutic targets for neurogenic bladder fibrosis via multi-omics analysis.
    Article Snippet: Inflammatory responses play a crucial role in the progression of pediatric neurogenic bladder (NB)-associated fibrosis; however, their specific contributions remain poorly understood.. This study aimed to identify inflammation-related biomarkers for diagnosing NB fibrosis and to explore potential therapeutic drugs.. The NB rat model was established by spinal nerve severing, followed by single-cell and bulk RNA sequencing of bladder tissues.

    Article Title: Low-Dose Trans -Resveratrol Ameliorates Diabetes-Induced Retinal Ganglion Cell Degeneration via TyrRS/c-Jun Pathway
    Article Snippet: Slides were incubated in diluent primary antibody overnight at 4°C, washed with PBS 3 times, and incubated in diluent containing Alexa Fluor 488/594-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature. .. After DAPI staining for 5 minutes and another 3 washes with PBS, the slides were covered in AntiFade Mounting Medium (MCE) and then observed under a fluorescence microscope (Olympus BX53) or a confocal microscope (C2; Nikon, Tokyo, Japan). ..

    Article Title: A proteostasis clock underlies the timing of bacterial dormancy and antibiotic tolerance
    Article Snippet: .. For DAPI staining, fixed cells were stained with DAPI (MCE, HY-D0814) at a concentration of 1 μg/mL in PBS for 10 minutes at room temperature. ..

    Immunofluorescence:

    Article Title: Imipramine inhibits feline infectious peritonitis virus infection by targeting host cholesterol metabolism via NPC1.
    Article Snippet: .. The procedure closely followed that of the immunofluorescence assay, with the exception that DAPI staining was replaced by staining with 0.5 mg/mL Filipin complex (MedChemExpress, USA), which specifically binds to unesterified cholesterol. ..

    Fluorescence:

    Article Title: The landscape of cell regulatory and communication networks in the human dental follicle
    Article Snippet: Embedded in the O.C.T compound (4583, Sakura Finetek, United States), tissues were made to obtain 10-μm frozen sections by a freezing microtome (Leica, Germany). .. After permeabilization with 0.3% Triton X-100 (X100PC, Sigma-Aldrich, United States) and blocking with the goat serum (AR0009, BOSTER, China), sections were incubated with primary antibodies for FOXO3 (12829, CST, United States; diluted 1:400), RUNX3 (sc-376591, Santa Cruz, United States; diluted 1:100), CD31 (FAB3628G, R&D Systems, United States; diluted 1:100), CD31 (3528, CST, United States; diluted 1:400), F4/80 (14-4801-82, eBioscience, United States; diluted 1:100), COL1 (72026, CST, United States; diluted 1:100), CD44-PE (553134, BD Pharmingen, United States; diluted 1:100), CD20 (sc-7735, Santa Cruz, United States; diluted 1:100), ANGPTL (sc-365146, Santa Cruz, United States; diluted 1:100), and ITGB-FITC (11-0299-42, eBioscience, United States; diluted 1:100) at 4°C for 14 h. Fluorescence-conjugated secondary antibodies were then stained at 37°C for 1 h, followed by DAPI staining for 10 min or directly sealing slides with AntiFade Mounting Medium (HY-K1042, MedChemExpress, United States). .. The fluorescence images were captured with CLSM (Nikon, Japan).

    Article Title: Low-Dose Trans -Resveratrol Ameliorates Diabetes-Induced Retinal Ganglion Cell Degeneration via TyrRS/c-Jun Pathway
    Article Snippet: Slides were incubated in diluent primary antibody overnight at 4°C, washed with PBS 3 times, and incubated in diluent containing Alexa Fluor 488/594-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature. .. After DAPI staining for 5 minutes and another 3 washes with PBS, the slides were covered in AntiFade Mounting Medium (MCE) and then observed under a fluorescence microscope (Olympus BX53) or a confocal microscope (C2; Nikon, Tokyo, Japan). ..

    Construct:

    Article Title: Engineering a Mechanoactive Fibrous Substrate with Enhanced Efficiency in Regulating Stem Cell Tenodifferentiation.
    Article Snippet: Electrospun-aligned fibers in ultrathin fineness have previously demonstrated a limited capacity in driving stem cells to differentiate into tendon-like cells.. In view of the tendon’s mechanoactive nature, endowing such aligned fibrous structure with mechanoactivity to exert in situ mechanical stimulus by itself, namely, without any forces externally applied, is likely to potentiate its efficiency of tenogenic induction.. To test this hypothesis, in this study, a shape-memory-capable poly(L-lactide-co-caprolactone) (PLCL) copolymer was electrospun into aligned fibrous form followed by a “stretching-recovery” shape-programming procedure to impart shape memory capability.

    Concentration Assay:

    Article Title: A proteostasis clock underlies the timing of bacterial dormancy and antibiotic tolerance
    Article Snippet: .. For DAPI staining, fixed cells were stained with DAPI (MCE, HY-D0814) at a concentration of 1 μg/mL in PBS for 10 minutes at room temperature. ..



    Similar Products

    98
    MedChemExpress dapi staining
    Dapi Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/DAPI/pm42365506-96-13-24
    Average 98 stars, based on 1 article reviews
    dapi staining - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    97
    Thermo Fisher dihydrochloride dapi staining
    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
    Dihydrochloride Dapi Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/4'%2C6'-DIAMIDINO-2-PHENYLINDOLE+DIHYDROCHLORIDE/pmc13112262-66-41-44
    Average 97 stars, based on 1 article reviews
    dihydrochloride dapi staining - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    99
    Beyotime dapi staining solution
    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
    Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/DAPI+Staining+Solution/pmc13097096-64-0-21
    Average 99 stars, based on 1 article reviews
    dapi staining solution - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Servicebio Inc diamidino 2 phenylindole dapi staining
    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
    Diamidino 2 Phenylindole Dapi Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/2+4+6+diamidino+phenylindole/pmc13049910-67-22-29
    Average 86 stars, based on 1 article reviews
    diamidino 2 phenylindole dapi staining - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Servicebio Inc dapi staining solution
    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
    Dapi Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/dapi+solution/pm42296610-74-1-4
    Average 86 stars, based on 1 article reviews
    dapi staining solution - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Sangon Biotech dapi stain solution
    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
    Dapi Stain Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/dapi+solution+staining/pmc13138055-43-0-4
    Average 86 stars, based on 1 article reviews
    dapi stain solution - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    10X Genomics diamidino 2 phenylindole dapi staining
    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
    Diamidino 2 Phenylindole Dapi Staining, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/dapi/pm42135486-420-0-17
    Average 86 stars, based on 1 article reviews
    diamidino 2 phenylindole dapi staining - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Journal: Biomaterials and Biosystems

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

    doi: 10.1016/j.bbiosy.2026.100137

    Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

    Techniques: Staining, Cell Culture