cell staining reagents (Beyotime)

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Beyotime cell staining reagents
Cell Staining Reagents, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32539 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+reagent/DAPI/pmc13098730-37-0-14
Average 99 stars, based on 32539 article reviews

cell staining reagents - by Bioz Stars, 2026-10

99/100 stars

Images

Staining:

Article Title: Farnesyltransferase inhibitor LB42708 disables oncogenic RAS signaling and overcomes gefitinib resistance in NSCLC via FTase α-subunit and RAS degradation.
Article Snippet: .. DAPI reagent (C1005, Beyotime Biotechnology, Shanghai, China) was then applied for nuclear staining. .. Images were captured using a fluorescence microscope (Olympus, Tokyo, Japan), and the proportion of EdUpositive cells was quantified using ImageJ software (NIH, NY, USA).

Article Title: Multifunctional hollow manganese silicate nanoparticles for the treatment of acute lung injury and Pseudomonas aeruginosa pulmonary infection.
Article Snippet: Acute respiratory distress syndrome (ARDS) is a severe inflammatory lung condition associated with high morbidity and mortality, underscoring the urgent need for effective treatment options.. This study proposes a nanotherapeutic strategy for acute lung injury (ALI) based on manganese silicate (MS) hollow nanospheres, whose intrinsic properties include ROS scavenging enabled by multivalent Mn, and immunomodulatory and tissue-protective effects endowed by the bioactive ions released from MS.. The designed hollow structure of MS grants the nanoparticles with drug delivery capability.

Article Title: Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regeneration
Article Snippet: .. IHC staining involved inhibiting endogenous peroxidase activity with hydrogen peroxide solution and blocking the sections with a 3 % bovine serum albumin solution for 30 min. Primary antibodies (Bioss: HSPA5, bs-1219R; IRE1, bs-8680R; phospho-IRE1α, bs-4308R; PERK, bs-2469R; phospho-PERK, bs-3330R; ATF6, bs-1634R; Caspase-12, bs-0967R; FHOD3, bs-13156R, CN; Proteintech: CHOP, 15204-1-AP, CN; Thermo Fisher Scientific: IL-6, MA5-51298, US) were added and incubated overnight at 4 °C, followed by incubation with secondary antibodies for 1 h. Nuclei were stained with DAPI reagent (C1005, Beyotime, CN), and the sections were mounted with glycerol. .. The samples were observed and photographed using an upright fluorescence microscope (DM2500, Leica).

Article Title: NSDHL as a regulator of lipid peroxidation and ER stress-mediated apoptosis in ovarian cancer.
Article Snippet: .. Finally, DAPI reagent (Beyotime, P0131) was employed to stain cell nuclei. .. Images were visualized using Olympus FLUOVIEW FV1200 laser scanning microscope.

Article Title: TRAF6 promotes the ferroptosis defense through AKT/mitochondria damage in KRAS-driven lung cancer.
Article Snippet: Ferroptosis, an iron-dependent form of cell death, has emerged as a therapeutic vulnerability in cancer, yet its regulation in kirsten rat sarcoma viral oncogene homolog (KRAS)-mutant lung cancer remains poorly defined.. Here we identified tumor necrosis factor receptor-associated factor 6 (TRAF6) as a critical driver of mitochondria-dependent ferroptosis in KRAS-driven lung cancer.. TRAF6 inhibition increased lipid peroxidation, mitochondrial reactive oxygen species (ROS), and Fe2+ accumulation, thereby exacerbating mitochondrial damage and enhancing RAS-selective lethal 3 (RSL3)-induced ferroptosis.

Article Title: Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regeneration
Article Snippet: .. IHC staining involved inhibiting endogenous peroxidase activity with hydrogen peroxide solution and blocking the sections with a 3 % bovine serum albumin solution for 30 min. Primary antibodies (Bioss: HSPA5, bs-1219R; IRE1, bs-8680R; phospho-IRE1α, bs-4308R; PERK, bs-2469R; phospho-PERK, bs-3330R; ATF6, bs-1634R; Caspase12, bs-0967R; FHOD3, bs-13156R, CN; Proteintech: CHOP, 15204-1-AP, CN; Thermo Fisher Scientific: IL-6, MA5-51298, US) were added and incubated overnight at 4 ◦C, followed by incubation with secondary antibodies for 1 h. Nuclei were stained with DAPI reagent (C1005, Beyotime, CN), and the sections were mounted with glycerol. .. The samples were observed and photographed using an upright fluorescence microscope (DM2500, Leica).

Article Title: Farnesyltransferase inhibitor LB42708 disables oncogenic RAS signaling and overcomes gefitinib resistance in NSCLC via FTase α-subunit and RAS degradation
Article Snippet: .. DAPI reagent (C1005, Beyotime Biotechnology, Shanghai, China) was then applied for nuclear staining. .. Images were captured using a fluorescence microscope (Olympus, Tokyo, Japan), and the proportion of EdU-positive cells was quantified using ImageJ software (NIH, NY, USA).

Incubation:

Article Title: SIRT5 mediates the pro-osteogenic effects of estrogen through FDX1 demalonylation and cuproptosis inhibition in mesenchymal stem cells
Article Snippet: .. Mito-Tracker Red (C1032, 234 Beyotime) was added to the cells and incubated at 37 °C for 30 min. Nuclear location 235 was subsequently performed using DAPI reagent (C1006, Beyotime). .. Images were 236 taken using a Leica TCS SP5 Confocal Microscope (Leica, Wetzlar, Germany).

Article Title: Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regeneration
Article Snippet: .. IHC staining involved inhibiting endogenous peroxidase activity with hydrogen peroxide solution and blocking the sections with a 3 % bovine serum albumin solution for 30 min. Primary antibodies (Bioss: HSPA5, bs-1219R; IRE1, bs-8680R; phospho-IRE1α, bs-4308R; PERK, bs-2469R; phospho-PERK, bs-3330R; ATF6, bs-1634R; Caspase12, bs-0967R; FHOD3, bs-13156R, CN; Proteintech: CHOP, 15204-1-AP, CN; Thermo Fisher Scientific: IL-6, MA5-51298, US) were added and incubated overnight at 4 ◦C, followed by incubation with secondary antibodies for 1 h. Nuclei were stained with DAPI reagent (C1005, Beyotime, CN), and the sections were mounted with glycerol. .. The samples were observed and photographed using an upright fluorescence microscope (DM2500, Leica).

Immunohistochemistry:

Article Title: Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regeneration
Article Snippet: .. IHC staining involved inhibiting endogenous peroxidase activity with hydrogen peroxide solution and blocking the sections with a 3 % bovine serum albumin solution for 30 min. Primary antibodies (Bioss: HSPA5, bs-1219R; IRE1, bs-8680R; phospho-IRE1α, bs-4308R; PERK, bs-2469R; phospho-PERK, bs-3330R; ATF6, bs-1634R; Caspase12, bs-0967R; FHOD3, bs-13156R, CN; Proteintech: CHOP, 15204-1-AP, CN; Thermo Fisher Scientific: IL-6, MA5-51298, US) were added and incubated overnight at 4 ◦C, followed by incubation with secondary antibodies for 1 h. Nuclei were stained with DAPI reagent (C1005, Beyotime, CN), and the sections were mounted with glycerol. .. The samples were observed and photographed using an upright fluorescence microscope (DM2500, Leica).

Activity Assay:

Article Title: Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regeneration
Article Snippet: .. IHC staining involved inhibiting endogenous peroxidase activity with hydrogen peroxide solution and blocking the sections with a 3 % bovine serum albumin solution for 30 min. Primary antibodies (Bioss: HSPA5, bs-1219R; IRE1, bs-8680R; phospho-IRE1α, bs-4308R; PERK, bs-2469R; phospho-PERK, bs-3330R; ATF6, bs-1634R; Caspase12, bs-0967R; FHOD3, bs-13156R, CN; Proteintech: CHOP, 15204-1-AP, CN; Thermo Fisher Scientific: IL-6, MA5-51298, US) were added and incubated overnight at 4 ◦C, followed by incubation with secondary antibodies for 1 h. Nuclei were stained with DAPI reagent (C1005, Beyotime, CN), and the sections were mounted with glycerol. .. The samples were observed and photographed using an upright fluorescence microscope (DM2500, Leica).

Blocking Assay:

Article Title: Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regeneration
Article Snippet: .. IHC staining involved inhibiting endogenous peroxidase activity with hydrogen peroxide solution and blocking the sections with a 3 % bovine serum albumin solution for 30 min. Primary antibodies (Bioss: HSPA5, bs-1219R; IRE1, bs-8680R; phospho-IRE1α, bs-4308R; PERK, bs-2469R; phospho-PERK, bs-3330R; ATF6, bs-1634R; Caspase12, bs-0967R; FHOD3, bs-13156R, CN; Proteintech: CHOP, 15204-1-AP, CN; Thermo Fisher Scientific: IL-6, MA5-51298, US) were added and incubated overnight at 4 ◦C, followed by incubation with secondary antibodies for 1 h. Nuclei were stained with DAPI reagent (C1005, Beyotime, CN), and the sections were mounted with glycerol. .. The samples were observed and photographed using an upright fluorescence microscope (DM2500, Leica).

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