dab1 expression  (Millipore)


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  • 99
    Name:
    Anti DAB1 antibody
    Description:
    Anti DAB1 Antibody detects endogenous levels of total DAB1 protein
    Catalog Number:
    sab4503448
    Price:
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    Structured Review

    Millipore dab1 expression
    Anti DAB1 antibody
    Anti DAB1 Antibody detects endogenous levels of total DAB1 protein
    https://www.bioz.com/result/dab1 expression/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dab1 expression - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Dab1 (Disable Homolog-1) Reelin Adaptor Protein Is Overexpressed in the Olfactory Bulb at Early Postnatal Stages"

    Article Title: Dab1 (Disable Homolog-1) Reelin Adaptor Protein Is Overexpressed in the Olfactory Bulb at Early Postnatal Stages

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0026673

    Relative levels of both Reln and Dab1 proteins by western blot in the olfactory bulb. (a) Reln and Dab1 protein bands identified in cytoplasm (CF) and nuclear (NF) fractions of wt and reeler mice. Identities of protein bands are indicated. Reln is absent in reeler . Dab1 quantities are different in CF and NF identified by distinct band intensities. (b) Amount of Dab1 compared between wt and reeler animals in both subcellular compartments. (c) Comparison of relative levels of Dab1 in CF and NF of wt and reeler mice. CF amount of Dab1 is statistically higher in wt at P15, while in reeler the levels are higher at P7 and P15. In NF, Dab1 levels are statistically higher exclusively at P15. (d) Comparison of Dab1 quantities between wt and reeler animals. Dab1 reeler content is significantly higher in CF at all studied ages. In NF this difference is restricted to P15. (* p
    Figure Legend Snippet: Relative levels of both Reln and Dab1 proteins by western blot in the olfactory bulb. (a) Reln and Dab1 protein bands identified in cytoplasm (CF) and nuclear (NF) fractions of wt and reeler mice. Identities of protein bands are indicated. Reln is absent in reeler . Dab1 quantities are different in CF and NF identified by distinct band intensities. (b) Amount of Dab1 compared between wt and reeler animals in both subcellular compartments. (c) Comparison of relative levels of Dab1 in CF and NF of wt and reeler mice. CF amount of Dab1 is statistically higher in wt at P15, while in reeler the levels are higher at P7 and P15. In NF, Dab1 levels are statistically higher exclusively at P15. (d) Comparison of Dab1 quantities between wt and reeler animals. Dab1 reeler content is significantly higher in CF at all studied ages. In NF this difference is restricted to P15. (* p

    Techniques Used: Western Blot, Mouse Assay

    Characterization of periglomerular Dab1 neurons with calbindin (CB), parvalbumin (PV) and tyrosine-hydroxylase (TH) markers. Green color corresponds to Dab1 expression, while the red one corresponds to either CB (a–b, g–h, m–n, s–t), PV (c–d, i––j, o–p, u–v) or TH (e–f, k–l, q–r, w–x) at P0 (a–f), P3 (g–l), P7 (m–r) and P15 (s–x). None CB (a–b) and PV (c–d) positive cells express Dab1 at P0–P3. By contrast, whole TH cell populations express nuclear Dab1 at these ages (e–f, g–i). At P7 some CB and PV are positive for Dab1. By contrary, in reeler mice PV cells do not express Dab1 and the cell number is lower (m–p). At this age, TH cells show a lack of Dab1 nuclear expression, which become apparent in some of TH-fibers (q–r). Similar results are observed at P15 (s–x). Nuclei counterstained with Hoechst (blue). GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cell layer. Scale bar: 50 µm.
    Figure Legend Snippet: Characterization of periglomerular Dab1 neurons with calbindin (CB), parvalbumin (PV) and tyrosine-hydroxylase (TH) markers. Green color corresponds to Dab1 expression, while the red one corresponds to either CB (a–b, g–h, m–n, s–t), PV (c–d, i––j, o–p, u–v) or TH (e–f, k–l, q–r, w–x) at P0 (a–f), P3 (g–l), P7 (m–r) and P15 (s–x). None CB (a–b) and PV (c–d) positive cells express Dab1 at P0–P3. By contrast, whole TH cell populations express nuclear Dab1 at these ages (e–f, g–i). At P7 some CB and PV are positive for Dab1. By contrary, in reeler mice PV cells do not express Dab1 and the cell number is lower (m–p). At this age, TH cells show a lack of Dab1 nuclear expression, which become apparent in some of TH-fibers (q–r). Similar results are observed at P15 (s–x). Nuclei counterstained with Hoechst (blue). GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cell layer. Scale bar: 50 µm.

    Techniques Used: Expressing, Mouse Assay

    Identification of astroglial and oligodendroglial cell expressing Dab1. P0 (a–d), P3 (e–h), P7 (i–l) and P15 (m–p). Astrocytes are recognized by GFAP (a–b, e–f, i–j, m–n) whereas oligodendrocytes by RIP (c–d, g–h, k–l, o–p). At P0–P3, GFAP (red) is restricted to the ONL and GL (a–b, e–f) and onwards labels the remaining layers, being negative for Dab1 (i–j, m–n). RIP labeling (red) is restricted to non-myelin oligodendrocytes in the GcL at P0–P3 (c–d, g–h). At P7, RIP expression is located in myelin and non-myelin oligodendrocytes (k–l). Non-myelin oligodendrocytes are absent at P15 (o–p). In all cases oligodendrocytes are negatives for Dab1. Labeling pattern is indistinguishable between wt and reeler animals. Nuclei are counterstained with Hoechst (blue). ONL, olfactory nerve layer; GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cells layer; GcL, granular cells layer. Scale bar: 100 µm.
    Figure Legend Snippet: Identification of astroglial and oligodendroglial cell expressing Dab1. P0 (a–d), P3 (e–h), P7 (i–l) and P15 (m–p). Astrocytes are recognized by GFAP (a–b, e–f, i–j, m–n) whereas oligodendrocytes by RIP (c–d, g–h, k–l, o–p). At P0–P3, GFAP (red) is restricted to the ONL and GL (a–b, e–f) and onwards labels the remaining layers, being negative for Dab1 (i–j, m–n). RIP labeling (red) is restricted to non-myelin oligodendrocytes in the GcL at P0–P3 (c–d, g–h). At P7, RIP expression is located in myelin and non-myelin oligodendrocytes (k–l). Non-myelin oligodendrocytes are absent at P15 (o–p). In all cases oligodendrocytes are negatives for Dab1. Labeling pattern is indistinguishable between wt and reeler animals. Nuclei are counterstained with Hoechst (blue). ONL, olfactory nerve layer; GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cells layer; GcL, granular cells layer. Scale bar: 100 µm.

    Techniques Used: Expressing, Labeling

    Colocalization of Dab1/Reln and Dab1/NeuN in postnatal olfactory bulb cells. Dab1 (green) and Reln (red) in wild type (a–d); Dab1 and NeuN (red) in wild type (e, h, k, n) and reeler (f, i, l, o). Nuclei are counterstained with Hoechst (blue). Images correspond to representative sagittal sections at P0 (a, e, f, g), P3, (b, h, i, j), P7 (c, k, l, m) and P15 (d, n, o, p). Reln shows a complementary location pattern with Dab1 positive nuclei of mitral cells (a–d). At P0, glomeruli are slightly apparent with Reln staining (a). From P3 to P15 glomeruli strongly express Dab1 and periglomerular cells start to express both Reln and nuclear Dab1 (b–d). From P3 the majority of granular and periglomerular cells are positive for NeuN (e–p), many of them with nuclear Dab1 expression (arrows). Cartoons represent the bilayer formation by the mitral cells (nuclear Dab1 staining) with respect to the superficial granular cells (NeuN labeling). This cell positioning is disrupted in reeler mice (cartoons). ONL, olfactory nerve layer; GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cells layer; IPL, internal plexiform layer; GcL, granular cells layer; SEZ, subependymal zone. Scale bar: 100 µm.
    Figure Legend Snippet: Colocalization of Dab1/Reln and Dab1/NeuN in postnatal olfactory bulb cells. Dab1 (green) and Reln (red) in wild type (a–d); Dab1 and NeuN (red) in wild type (e, h, k, n) and reeler (f, i, l, o). Nuclei are counterstained with Hoechst (blue). Images correspond to representative sagittal sections at P0 (a, e, f, g), P3, (b, h, i, j), P7 (c, k, l, m) and P15 (d, n, o, p). Reln shows a complementary location pattern with Dab1 positive nuclei of mitral cells (a–d). At P0, glomeruli are slightly apparent with Reln staining (a). From P3 to P15 glomeruli strongly express Dab1 and periglomerular cells start to express both Reln and nuclear Dab1 (b–d). From P3 the majority of granular and periglomerular cells are positive for NeuN (e–p), many of them with nuclear Dab1 expression (arrows). Cartoons represent the bilayer formation by the mitral cells (nuclear Dab1 staining) with respect to the superficial granular cells (NeuN labeling). This cell positioning is disrupted in reeler mice (cartoons). ONL, olfactory nerve layer; GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cells layer; IPL, internal plexiform layer; GcL, granular cells layer; SEZ, subependymal zone. Scale bar: 100 µm.

    Techniques Used: Staining, Expressing, Labeling, Mouse Assay

    Pattern of Dab1 mRNA location in the postnatal olfactory bulb. Dab1 transcripts are shown in wt (a, c, e, g) and reeler (b, d, f, h) mice at P0 (a,b), P3, (c, d), P7 (e, f) and P15 (g, h). Transcripts are mainly detected in granular cells (black stars in GcL), mitral cells (white arrows in MCL) and several periglomerular cell populations and intra-glomeruli processes (black arrows in GL). No differences are observed between wt and reeler mice. Note the high presence of Dab1 mRNA in the rostral migratory stream (RMS) cells. Scale bar: 1 mm.
    Figure Legend Snippet: Pattern of Dab1 mRNA location in the postnatal olfactory bulb. Dab1 transcripts are shown in wt (a, c, e, g) and reeler (b, d, f, h) mice at P0 (a,b), P3, (c, d), P7 (e, f) and P15 (g, h). Transcripts are mainly detected in granular cells (black stars in GcL), mitral cells (white arrows in MCL) and several periglomerular cell populations and intra-glomeruli processes (black arrows in GL). No differences are observed between wt and reeler mice. Note the high presence of Dab1 mRNA in the rostral migratory stream (RMS) cells. Scale bar: 1 mm.

    Techniques Used: Mouse Assay

    Colocalization of Dab1/Map2a,b and Dab1/RC2 in postnatal olfactory bulb cells. Dab1 (green), Map2a,b (red) (a–b, f–g, k–l, p–q) and the radial glial cell marker RC2 (red) (c–e, h–j, m–o, r–t). OB sagittal sections at P0 (a–e), P3 (f–j), P7 (k–o) and P15 (p–t). At P0 neuronal Dab1 positive processes (Map2a,b) are evident in the GL, EPL and MCL (a, b) while radial glial Dab1 positive processes (RC2) occupied the OB surface (c–e). From P3 onwards, neuronal Dab1 positive neuronal fibers become evident in the IPL and GcL (f–g) while the radial cell processes decreased in quantity. Some Rc2 positive/Dab1 negative cells surround the glomeruli (h–j). Neuronal fibers pattern is similar at P7 (k–i) and P15 (p–q), whereas Rc2 fibers are absent from P7 (m–o). From P7 to P15 all radial glial cells are Dab1 negative (m–o; r–t). Nuclei counterstained with Hoechst (blue). ONL, olfactory nerve layer; GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cells layer; IPL, internal plexiform layer; GcL, granular cells layer; SEZ, subependymal zone. Scale bar: 100 µm.
    Figure Legend Snippet: Colocalization of Dab1/Map2a,b and Dab1/RC2 in postnatal olfactory bulb cells. Dab1 (green), Map2a,b (red) (a–b, f–g, k–l, p–q) and the radial glial cell marker RC2 (red) (c–e, h–j, m–o, r–t). OB sagittal sections at P0 (a–e), P3 (f–j), P7 (k–o) and P15 (p–t). At P0 neuronal Dab1 positive processes (Map2a,b) are evident in the GL, EPL and MCL (a, b) while radial glial Dab1 positive processes (RC2) occupied the OB surface (c–e). From P3 onwards, neuronal Dab1 positive neuronal fibers become evident in the IPL and GcL (f–g) while the radial cell processes decreased in quantity. Some Rc2 positive/Dab1 negative cells surround the glomeruli (h–j). Neuronal fibers pattern is similar at P7 (k–i) and P15 (p–q), whereas Rc2 fibers are absent from P7 (m–o). From P7 to P15 all radial glial cells are Dab1 negative (m–o; r–t). Nuclei counterstained with Hoechst (blue). ONL, olfactory nerve layer; GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cells layer; IPL, internal plexiform layer; GcL, granular cells layer; SEZ, subependymal zone. Scale bar: 100 µm.

    Techniques Used: Marker

    Detection of Dab1 protein within cell nuclei. Labeling of isolated nuclei and tissue sections of the OB in wild type (a, c, e, g, i, k, m, o) and reeler (b, d, f, h, j, l, n, p) at P0 (a, b, e, f), P3 (c, d, g, h), P7 (i, j, m, n) and P15 (k, l, o, p). Labeling shows a green dotted pattern in nuclei from both isolated (a–d, i–l) and in vivo sections (e–h, m–p). Nuclei are counterstained with Hoechst (blue). In vivo images are single confocal sections from MCL (mitral cells layer). Scales bars: 10 µm in e–h, m–p; 5 µm in a–d, i–l.
    Figure Legend Snippet: Detection of Dab1 protein within cell nuclei. Labeling of isolated nuclei and tissue sections of the OB in wild type (a, c, e, g, i, k, m, o) and reeler (b, d, f, h, j, l, n, p) at P0 (a, b, e, f), P3 (c, d, g, h), P7 (i, j, m, n) and P15 (k, l, o, p). Labeling shows a green dotted pattern in nuclei from both isolated (a–d, i–l) and in vivo sections (e–h, m–p). Nuclei are counterstained with Hoechst (blue). In vivo images are single confocal sections from MCL (mitral cells layer). Scales bars: 10 µm in e–h, m–p; 5 µm in a–d, i–l.

    Techniques Used: Labeling, Isolation, In Vivo

    Dab1 protein expression in the postnatal olfactory bulb. Protein expression in wild type (a, c, e, g) and reeler (b, d, f, h) mice at P0 (a, b), P3, (c, d), P7 (e, f) and P15 (g, h) . Dab1 immunostaining is strongly localized in cell processes of the GL, EPL, and IPL. A dotted pattern is evident in GcL. Mitral and some periglomerular cells are easily identified by a characteristic nuclear Dab1 labeling. Nuclei labeling in periglomerular cells is evident from P3. No marked differences are observed between wt and reeler at these ages. Nuclei are counterstained with Hoechst (blue). Images represent sagittal sections of OB. Scale bars a-h: 100 µm; inset images: 1 mm.
    Figure Legend Snippet: Dab1 protein expression in the postnatal olfactory bulb. Protein expression in wild type (a, c, e, g) and reeler (b, d, f, h) mice at P0 (a, b), P3, (c, d), P7 (e, f) and P15 (g, h) . Dab1 immunostaining is strongly localized in cell processes of the GL, EPL, and IPL. A dotted pattern is evident in GcL. Mitral and some periglomerular cells are easily identified by a characteristic nuclear Dab1 labeling. Nuclei labeling in periglomerular cells is evident from P3. No marked differences are observed between wt and reeler at these ages. Nuclei are counterstained with Hoechst (blue). Images represent sagittal sections of OB. Scale bars a-h: 100 µm; inset images: 1 mm.

    Techniques Used: Expressing, Mouse Assay, Immunostaining, Labeling

    Related Articles

    Immunohistochemistry:

    Article Title: Dab1 (Disable Homolog-1) Reelin Adaptor Protein Is Overexpressed in the Olfactory Bulb at Early Postnatal Stages
    Article Snippet: .. Dab1 expression using two different anti-Dab1 antibodies by western blot and immunohistochemistry. (a) Western blot of wt OB extracts using two anti-Dab1 antibodies from Chemicon and Sigma-Aldrich. .. Both labeled the specific 80 kDa band correspond to Dab1 protein.

    Immunodetection:

    Article Title: Role for Reelin in the Development of Granule Cell Dispersion in Temporal Lobe Epilepsy
    Article Snippet: .. Immunodetection of dab1 was performed by treatment with an anti-dab1 antibody (1:5000;Chemicon International, Temecula, CA) followed by incubation with an alkaline phosphatase-coupled secondary antibody and enhanced chemiluminescence for detection. .. In hippocampal sections of control tissue (Fig. a–c ), many reelin mRNA-expressing neurons were found by ISH.

    Blocking Assay:

    Article Title: Ectopic Reelin Induces Neuronal Aggregation with a Normal Birthdate-Dependent “Inside-Out” Alignment in the Developing Neocortex
    Article Snippet: .. The blot was treated for at least 1 h with a blocking buffer, TBS-T (50 m m Tris, pH 7.6, 150 m m NaCl, 0.05% Tween 20), containing 5% nonfat dry milk, and after incubating for 1 h with a rabbit anti-Dab1 antibody (1:500, Millipore Bioscience Research Reagents) or a mouse anti-HA antibody (1:2000, Covance) and washing 3 times, it was incubated for 1 h with an HRP-labeled goat anti-rabbit IgG (1:500, DAKO) and washed 3 times again. ..

    Incubation:

    Article Title: Role for Reelin in the Development of Granule Cell Dispersion in Temporal Lobe Epilepsy
    Article Snippet: .. Immunodetection of dab1 was performed by treatment with an anti-dab1 antibody (1:5000;Chemicon International, Temecula, CA) followed by incubation with an alkaline phosphatase-coupled secondary antibody and enhanced chemiluminescence for detection. .. In hippocampal sections of control tissue (Fig. a–c ), many reelin mRNA-expressing neurons were found by ISH.

    Article Title: Ectopic Reelin Induces Neuronal Aggregation with a Normal Birthdate-Dependent “Inside-Out” Alignment in the Developing Neocortex
    Article Snippet: .. The blot was treated for at least 1 h with a blocking buffer, TBS-T (50 m m Tris, pH 7.6, 150 m m NaCl, 0.05% Tween 20), containing 5% nonfat dry milk, and after incubating for 1 h with a rabbit anti-Dab1 antibody (1:500, Millipore Bioscience Research Reagents) or a mouse anti-HA antibody (1:2000, Covance) and washing 3 times, it was incubated for 1 h with an HRP-labeled goat anti-rabbit IgG (1:500, DAKO) and washed 3 times again. ..

    Expressing:

    Article Title: Dab1 (Disable Homolog-1) Reelin Adaptor Protein Is Overexpressed in the Olfactory Bulb at Early Postnatal Stages
    Article Snippet: .. Dab1 expression using two different anti-Dab1 antibodies by western blot and immunohistochemistry. (a) Western blot of wt OB extracts using two anti-Dab1 antibodies from Chemicon and Sigma-Aldrich. .. Both labeled the specific 80 kDa band correspond to Dab1 protein.

    Western Blot:

    Article Title: Dab1 (Disable Homolog-1) Reelin Adaptor Protein Is Overexpressed in the Olfactory Bulb at Early Postnatal Stages
    Article Snippet: .. Dab1 expression using two different anti-Dab1 antibodies by western blot and immunohistochemistry. (a) Western blot of wt OB extracts using two anti-Dab1 antibodies from Chemicon and Sigma-Aldrich. .. Both labeled the specific 80 kDa band correspond to Dab1 protein.

    Immunofluorescence:

    Article Title: Rbx2 regulates neuronal migration through different Cullin5-RING ligase adaptors
    Article Snippet: .. The following antibodies were used for immunofluorescence: rabbit anti-CDP (Cux1, Santa Cruz Biotechnology); rat anti-Ctip2 and rabbit anti-Tbr1 (Abcam); rabbit anti-Calbindin and mouse anti-Reelin (Calbiochem); rabbit anti-pS.H3 (Cell Signaling); mouse anti-Nestin and anti-GM130 (BD Bioscience); mouse anti-Lhx1/5 (4F2) (Developmental Studies Hybridoma Bank); and rabbit anti-Dab1 (Millipore). .. Binding of pY.Dab1 to different SOCS proteins was assayed in vitro.

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  • 94
    Millipore rabbit anti dab1
    SOCS7 promotes <t>Dab1</t> polyubiquitylation and degradation
    Rabbit Anti Dab1, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti dab1/product/Millipore
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit anti dab1 - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    90
    Millipore dab1
    <t>Dab1</t> interacts with Csmd2 via FENPMY motif. A. Schematic of Dab1 protein, noting the phosphotyrosine binding (PTB) domain through which Dab1 docks to the plasma membrane and simultaneously binds F/Y-D/E-NPxY motifs of protein interactors. B. Known interactors of Dab1 and their corresponding Dab1-binding motifs. Csmd protein family members each contain an FENPMY motif. C. mRNA in situ hybridization of E14.5 mouse forebrains comparing expression of Csmd1 , Csmd2 , and Csmd3 to that of Dab1 . Of the three Csmd mRNAs, the Csmd2 mRNA expression pattern appears to match that of Dab1 mRNA most closely. Dab1 in situ image taken from the GenePaint database, set ID MH356, image C997.6.1.B. D. Schematic of FLAG-tagged Csmd2 constructs generated for in vitro characterization of Csmd2-Dab1 interaction. Immunoprecipitation of FLAG-Csmd2 from transfected HEK293T cells pulls down co-transfected Dab1, but this interaction is abolished when the NPMY motif in Csmd2 is mutated to AAAA.
    Dab1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dab1/product/Millipore
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dab1 - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    SOCS7 promotes Dab1 polyubiquitylation and degradation

    Journal: Developmental cell

    Article Title: Rbx2 regulates neuronal migration through different Cullin5-RING ligase adaptors

    doi: 10.1016/j.devcel.2013.09.022

    Figure Lengend Snippet: SOCS7 promotes Dab1 polyubiquitylation and degradation

    Article Snippet: The following antibodies were used for immunofluorescence: rabbit anti-CDP (Cux1, Santa Cruz Biotechnology); rat anti-Ctip2 and rabbit anti-Tbr1 (Abcam); rabbit anti-Calbindin and mouse anti-Reelin (Calbiochem); rabbit anti-pS.H3 (Cell Signaling); mouse anti-Nestin and anti-GM130 (BD Bioscience); mouse anti-Lhx1/5 (4F2) (Developmental Studies Hybridoma Bank); and rabbit anti-Dab1 (Millipore).

    Techniques:

    Rbx2 regulates Dab1 in cortical neurons and Purkinje cells

    Journal: Developmental cell

    Article Title: Rbx2 regulates neuronal migration through different Cullin5-RING ligase adaptors

    doi: 10.1016/j.devcel.2013.09.022

    Figure Lengend Snippet: Rbx2 regulates Dab1 in cortical neurons and Purkinje cells

    Article Snippet: The following antibodies were used for immunofluorescence: rabbit anti-CDP (Cux1, Santa Cruz Biotechnology); rat anti-Ctip2 and rabbit anti-Tbr1 (Abcam); rabbit anti-Calbindin and mouse anti-Reelin (Calbiochem); rabbit anti-pS.H3 (Cell Signaling); mouse anti-Nestin and anti-GM130 (BD Bioscience); mouse anti-Lhx1/5 (4F2) (Developmental Studies Hybridoma Bank); and rabbit anti-Dab1 (Millipore).

    Techniques:

    Rbx2 phenotype requires Dab1

    Journal: Developmental cell

    Article Title: Rbx2 regulates neuronal migration through different Cullin5-RING ligase adaptors

    doi: 10.1016/j.devcel.2013.09.022

    Figure Lengend Snippet: Rbx2 phenotype requires Dab1

    Article Snippet: The following antibodies were used for immunofluorescence: rabbit anti-CDP (Cux1, Santa Cruz Biotechnology); rat anti-Ctip2 and rabbit anti-Tbr1 (Abcam); rabbit anti-Calbindin and mouse anti-Reelin (Calbiochem); rabbit anti-pS.H3 (Cell Signaling); mouse anti-Nestin and anti-GM130 (BD Bioscience); mouse anti-Lhx1/5 (4F2) (Developmental Studies Hybridoma Bank); and rabbit anti-Dab1 (Millipore).

    Techniques:

    SOCS6 and 7 bind Dab1 and SOCS7 inhibits over-migration

    Journal: Developmental cell

    Article Title: Rbx2 regulates neuronal migration through different Cullin5-RING ligase adaptors

    doi: 10.1016/j.devcel.2013.09.022

    Figure Lengend Snippet: SOCS6 and 7 bind Dab1 and SOCS7 inhibits over-migration

    Article Snippet: The following antibodies were used for immunofluorescence: rabbit anti-CDP (Cux1, Santa Cruz Biotechnology); rat anti-Ctip2 and rabbit anti-Tbr1 (Abcam); rabbit anti-Calbindin and mouse anti-Reelin (Calbiochem); rabbit anti-pS.H3 (Cell Signaling); mouse anti-Nestin and anti-GM130 (BD Bioscience); mouse anti-Lhx1/5 (4F2) (Developmental Studies Hybridoma Bank); and rabbit anti-Dab1 (Millipore).

    Techniques: Migration

    SOCS7 regulates Dab1 levels and cortical but not cerebellar development

    Journal: Developmental cell

    Article Title: Rbx2 regulates neuronal migration through different Cullin5-RING ligase adaptors

    doi: 10.1016/j.devcel.2013.09.022

    Figure Lengend Snippet: SOCS7 regulates Dab1 levels and cortical but not cerebellar development

    Article Snippet: The following antibodies were used for immunofluorescence: rabbit anti-CDP (Cux1, Santa Cruz Biotechnology); rat anti-Ctip2 and rabbit anti-Tbr1 (Abcam); rabbit anti-Calbindin and mouse anti-Reelin (Calbiochem); rabbit anti-pS.H3 (Cell Signaling); mouse anti-Nestin and anti-GM130 (BD Bioscience); mouse anti-Lhx1/5 (4F2) (Developmental Studies Hybridoma Bank); and rabbit anti-Dab1 (Millipore).

    Techniques:

    Dab1 interacts with Csmd2 via FENPMY motif. A. Schematic of Dab1 protein, noting the phosphotyrosine binding (PTB) domain through which Dab1 docks to the plasma membrane and simultaneously binds F/Y-D/E-NPxY motifs of protein interactors. B. Known interactors of Dab1 and their corresponding Dab1-binding motifs. Csmd protein family members each contain an FENPMY motif. C. mRNA in situ hybridization of E14.5 mouse forebrains comparing expression of Csmd1 , Csmd2 , and Csmd3 to that of Dab1 . Of the three Csmd mRNAs, the Csmd2 mRNA expression pattern appears to match that of Dab1 mRNA most closely. Dab1 in situ image taken from the GenePaint database, set ID MH356, image C997.6.1.B. D. Schematic of FLAG-tagged Csmd2 constructs generated for in vitro characterization of Csmd2-Dab1 interaction. Immunoprecipitation of FLAG-Csmd2 from transfected HEK293T cells pulls down co-transfected Dab1, but this interaction is abolished when the NPMY motif in Csmd2 is mutated to AAAA.

    Journal: bioRxiv

    Article Title: Csmd2 interacts with Dab1 and is Required in Reelin-Mediated Neuronal Maturation

    doi: 10.1101/2020.01.30.925537

    Figure Lengend Snippet: Dab1 interacts with Csmd2 via FENPMY motif. A. Schematic of Dab1 protein, noting the phosphotyrosine binding (PTB) domain through which Dab1 docks to the plasma membrane and simultaneously binds F/Y-D/E-NPxY motifs of protein interactors. B. Known interactors of Dab1 and their corresponding Dab1-binding motifs. Csmd protein family members each contain an FENPMY motif. C. mRNA in situ hybridization of E14.5 mouse forebrains comparing expression of Csmd1 , Csmd2 , and Csmd3 to that of Dab1 . Of the three Csmd mRNAs, the Csmd2 mRNA expression pattern appears to match that of Dab1 mRNA most closely. Dab1 in situ image taken from the GenePaint database, set ID MH356, image C997.6.1.B. D. Schematic of FLAG-tagged Csmd2 constructs generated for in vitro characterization of Csmd2-Dab1 interaction. Immunoprecipitation of FLAG-Csmd2 from transfected HEK293T cells pulls down co-transfected Dab1, but this interaction is abolished when the NPMY motif in Csmd2 is mutated to AAAA.

    Article Snippet: For this reason, we focused our attention on the relationship between Dab1 and Csmd2 in this study.

    Techniques: Binding Assay, In Situ Hybridization, Expressing, In Situ, Construct, Generated, In Vitro, Immunoprecipitation, Transfection

    Mutation of Dab1-binding region does not affect the localization of Csmd2 at postsynaptic dendritic spines. A. Experimental design to visualize the localization of Csmd2 upon mutation of Dab1-binding FENPMY motif. FLAG-tagged wild-type and FENPMY mutant Csmd2 constructs were introduced into mouse forebrain progenitors at E14.5 by in utero electroporation. Electroporated brains were subsequently used at P7 or P60 for biochemical and immunohistochemical analyses. B. PSD-fraction enrichment by Triton X-100 extraction of crude synaptosomal fractions obtained from experiments outlined in A. Western blots reveal no difference in PSD enrichment between FLAG-Csmd2 wildtype and FENPMY mutant constructs. C. Immunohistochemical analysis for Csmd2 and PSD-95 confirmed no difference in dendritic spine localization between the two experiment groups. Scale bars = 10 μm.

    Journal: bioRxiv

    Article Title: Csmd2 interacts with Dab1 and is Required in Reelin-Mediated Neuronal Maturation

    doi: 10.1101/2020.01.30.925537

    Figure Lengend Snippet: Mutation of Dab1-binding region does not affect the localization of Csmd2 at postsynaptic dendritic spines. A. Experimental design to visualize the localization of Csmd2 upon mutation of Dab1-binding FENPMY motif. FLAG-tagged wild-type and FENPMY mutant Csmd2 constructs were introduced into mouse forebrain progenitors at E14.5 by in utero electroporation. Electroporated brains were subsequently used at P7 or P60 for biochemical and immunohistochemical analyses. B. PSD-fraction enrichment by Triton X-100 extraction of crude synaptosomal fractions obtained from experiments outlined in A. Western blots reveal no difference in PSD enrichment between FLAG-Csmd2 wildtype and FENPMY mutant constructs. C. Immunohistochemical analysis for Csmd2 and PSD-95 confirmed no difference in dendritic spine localization between the two experiment groups. Scale bars = 10 μm.

    Article Snippet: For this reason, we focused our attention on the relationship between Dab1 and Csmd2 in this study.

    Techniques: Mutagenesis, Binding Assay, Construct, In Utero, Electroporation, Immunohistochemistry, Western Blot