dab substrate powder 3 3 diaminobenzidine tetrahydrochloride  (Thermo Fisher)


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    DAB Substrate Powder 3 3 diaminobenzidine tetrahydrochloride
    Description:
    Thermo Scientific Pierce DAB enables chromogenic detection of horseradish peroxidase HRP activity based the action of 3 3 diaminobenzidine DAB in Western blot and tissue staining methods DAB 3 3 diaminobenzidine tetrahydrochloride MW 214 1 reacts with HRP in the presence of peroxide to yield an insoluble brown colored product at locations where peroxidase conjugated antibodies are bound to samples The brown precipitate is insoluble in alcohol and other organic solvents making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media Features of DAB Powder • HRP substrate for detection of horseradish peroxidase activity on solid media including nitrocellulose and PVDF membranes and fixed tissue samples • Chromogenic no special equipment needed for visualization the DAB reaction produces brown bands or spots at sites of reaction with HRP conjugated antibodies or probes • Package options choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use DAB has been used in a variety of applications including Western blotting immunohistochemistry and electron microscopy Many enhancement methods have been reported for DAB involving different buffer conditions the addition of metal ions and post treatment applications Ref 1 7 also see the Pierce Metal Enhanced DAB Substrate Kit Part No 34065 One disadvantage of DAB compared to other substrates is the need to use the working solution immediately because it begins a reaction process upon the addition of hydrogen peroxide This results in increased background color of the substrate However the brown bands resulting from DAB reactions do not fade as quickly as the color products of other precipitating HRP substrates Related Products Pierce DAB Substrate Kit
    Catalog Number:
    34001
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Cell Analysis|Cellular Imaging|IHC Staining & Detection|Immunohistochemistry (IHC)|Protein Assays and Analysis|Protein Biology|Western Blotting
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    Structured Review

    Thermo Fisher dab substrate powder 3 3 diaminobenzidine tetrahydrochloride
    Disruption of reactive oxygen species homeostasis in the rice osacbp6 mutant. a Relative expression of genes encoding class III peroxidases. Values are mean ± SD ( n = 3). b Comparison of peroxidase activities of the roots and shoots of 7-day-old seedlings and leaves of 21-day-old wild type (WT), osacbp6 and complemented line osacbp6 - C4 ( C4 ). Values are means ± SD ( n = 3). c Accumulation of superoxide anion (O 2 ∙- ) and hydrogen peroxide (H 2 O 2 ) in the leaf blades of osacbp6 . Leaf blades of 21-day-old WT and osacbp6 were subjected to nitroblue tetrazolium (NBT) staining to detect O 2 ∙- or <t>3,3′-diaminobenzidine</t> <t>tetrahydrochloride</t> (DAB) staining to detect H 2 O 2 in the dark for 3 and 24 h, respectively. Bars, 1 cm. d H 2 O 2 content in the roots and shoots of 7-day-old seedlings and leaves of 21-day-old WT, osacbp6 and C4 . Values are means ± SD ( n = 3). Asterisks indicate significant differences as evaluated by Student’s t -tests: * P
    Thermo Scientific Pierce DAB enables chromogenic detection of horseradish peroxidase HRP activity based the action of 3 3 diaminobenzidine DAB in Western blot and tissue staining methods DAB 3 3 diaminobenzidine tetrahydrochloride MW 214 1 reacts with HRP in the presence of peroxide to yield an insoluble brown colored product at locations where peroxidase conjugated antibodies are bound to samples The brown precipitate is insoluble in alcohol and other organic solvents making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media Features of DAB Powder • HRP substrate for detection of horseradish peroxidase activity on solid media including nitrocellulose and PVDF membranes and fixed tissue samples • Chromogenic no special equipment needed for visualization the DAB reaction produces brown bands or spots at sites of reaction with HRP conjugated antibodies or probes • Package options choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use DAB has been used in a variety of applications including Western blotting immunohistochemistry and electron microscopy Many enhancement methods have been reported for DAB involving different buffer conditions the addition of metal ions and post treatment applications Ref 1 7 also see the Pierce Metal Enhanced DAB Substrate Kit Part No 34065 One disadvantage of DAB compared to other substrates is the need to use the working solution immediately because it begins a reaction process upon the addition of hydrogen peroxide This results in increased background color of the substrate However the brown bands resulting from DAB reactions do not fade as quickly as the color products of other precipitating HRP substrates Related Products Pierce DAB Substrate Kit
    https://www.bioz.com/result/dab substrate powder 3 3 diaminobenzidine tetrahydrochloride/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dab substrate powder 3 3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "RICE ACYL-COA-BINDING PROTEIN6 Affects Acyl-CoA Homeostasis and Growth in Rice"

    Article Title: RICE ACYL-COA-BINDING PROTEIN6 Affects Acyl-CoA Homeostasis and Growth in Rice

    Journal: Rice

    doi: 10.1186/s12284-020-00435-y

    Disruption of reactive oxygen species homeostasis in the rice osacbp6 mutant. a Relative expression of genes encoding class III peroxidases. Values are mean ± SD ( n = 3). b Comparison of peroxidase activities of the roots and shoots of 7-day-old seedlings and leaves of 21-day-old wild type (WT), osacbp6 and complemented line osacbp6 - C4 ( C4 ). Values are means ± SD ( n = 3). c Accumulation of superoxide anion (O 2 ∙- ) and hydrogen peroxide (H 2 O 2 ) in the leaf blades of osacbp6 . Leaf blades of 21-day-old WT and osacbp6 were subjected to nitroblue tetrazolium (NBT) staining to detect O 2 ∙- or 3,3′-diaminobenzidine tetrahydrochloride (DAB) staining to detect H 2 O 2 in the dark for 3 and 24 h, respectively. Bars, 1 cm. d H 2 O 2 content in the roots and shoots of 7-day-old seedlings and leaves of 21-day-old WT, osacbp6 and C4 . Values are means ± SD ( n = 3). Asterisks indicate significant differences as evaluated by Student’s t -tests: * P
    Figure Legend Snippet: Disruption of reactive oxygen species homeostasis in the rice osacbp6 mutant. a Relative expression of genes encoding class III peroxidases. Values are mean ± SD ( n = 3). b Comparison of peroxidase activities of the roots and shoots of 7-day-old seedlings and leaves of 21-day-old wild type (WT), osacbp6 and complemented line osacbp6 - C4 ( C4 ). Values are means ± SD ( n = 3). c Accumulation of superoxide anion (O 2 ∙- ) and hydrogen peroxide (H 2 O 2 ) in the leaf blades of osacbp6 . Leaf blades of 21-day-old WT and osacbp6 were subjected to nitroblue tetrazolium (NBT) staining to detect O 2 ∙- or 3,3′-diaminobenzidine tetrahydrochloride (DAB) staining to detect H 2 O 2 in the dark for 3 and 24 h, respectively. Bars, 1 cm. d H 2 O 2 content in the roots and shoots of 7-day-old seedlings and leaves of 21-day-old WT, osacbp6 and C4 . Values are means ± SD ( n = 3). Asterisks indicate significant differences as evaluated by Student’s t -tests: * P

    Techniques Used: Mutagenesis, Expressing, Staining

    Related Articles

    Staining:

    Article Title: Downregulation of claudin-7 potentiates cellular proliferation and invasion in endometrial cancer
    Article Snippet: The slides were then incubated with primary anti-claudin-7 antibodies (1:1,000; Epitomics, Inc., Burlingame, CA, USA) overnight at 4°C. .. Antibody staining was visualized with 3,3′-diaminobenzidine (DAB; Invitrogen Life Technologies, Carlsbad, CA, USA). .. For evaluation, the following criteria were used: 0, no expression (complete negative staining); 1, weak expression (1–15% positive staining); 2, moderate expression (16–49% positive staining); and 3, strong expression (50–100% positive staining).

    Article Title: Glioblastoma Multiforme Cancer Stem Cells Express Components of the Renin–Angiotensin System
    Article Snippet: Immunohistochemical (IHC) staining was performed on the Leica Bond Rx autostainer (Leica, Nussloch, Germany) as previously described ( ). .. 3,3-Diaminobenzidine (DAB) IHC staining for SOX2 (1:500; cat# PA094, Thermo Fisher, Scientific, Scoresby, VIC, Australia), PRR (1:2000; cat# ab40790, Abcam, Cambridge, UK), ATIIR1 (1:30; cat# ab9391, Abcam), ATIIR2 (1:2000; cat# NBP1-77368, Novus Biologicals, LLC, Littleton, CO, USA), ACE (1:100; cat# MCA2054, AbD Serotec, Kidlington, UK) diluted with Bond™ primary antibody diluent (cat# AR9352, Leica) was done for all tissue samples. .. Immunofluorescent (IF) IHC staining was performed on two representative GBM tissue samples from the original cohort of patients used for DAB IHC staining, using identical primary antibodies and concentrations.

    Article Title: Central nervous system neurons acquire mast cell products via transgranulation
    Article Snippet: Both employed a biotinylated secondary antibody (Vector, Burlingame, CA, USA) and avidin-biotin-horseradish peroxidase (Vector). .. The first made use of conventional staining with 3,3′ diaminobenzidine (DAB) (Immunopure DAB, Pierce) and the second, a silver intensification followed by gold toning of the DAB product (not shown). ..

    Article Title: Severe acute respiratory syndrome coronavirus 3C‐like protease‐induced apoptosis
    Article Snippet: Each 100 μL of cell suspension was incubated with 5 μL of antiannexin V antibody (Biocompare, Abcom, Cambridge, UK) at room temperature for 15 min. .. The apoptotic cells were further stained using a horseradish peroxidase/diaminobenzidine (HRP/DAB) system of the SuperPicTure™ Polymer Detection Kit (Zymed Laboratories Inc., San Francisco, CA). .. The percentage of apoptotic cells with a permanent intense brown deposit was counted at × 200 magnification using bright field microscopy.

    Article Title: A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins
    Article Snippet: Cells were incubated with anti-myc 9E-10 antibody and goat anti-mouse secondary antibody (1:100) (HyClone). .. For immunostaining, cells at about 100% confluency were fixed with 2% paraformaldehyde-0.2% glutaraldehyde and stained with anti-myc (1:1,000) followed by anti-mouse secondary antibody and diaminobenzidine substrate (Lifetech). .. For FACS of transiently expressed CB5, A7 monolayers were transfected with pB5myc or vector only.

    Immunohistochemistry:

    Article Title: Glioblastoma Multiforme Cancer Stem Cells Express Components of the Renin–Angiotensin System
    Article Snippet: Immunohistochemical (IHC) staining was performed on the Leica Bond Rx autostainer (Leica, Nussloch, Germany) as previously described ( ). .. 3,3-Diaminobenzidine (DAB) IHC staining for SOX2 (1:500; cat# PA094, Thermo Fisher, Scientific, Scoresby, VIC, Australia), PRR (1:2000; cat# ab40790, Abcam, Cambridge, UK), ATIIR1 (1:30; cat# ab9391, Abcam), ATIIR2 (1:2000; cat# NBP1-77368, Novus Biologicals, LLC, Littleton, CO, USA), ACE (1:100; cat# MCA2054, AbD Serotec, Kidlington, UK) diluted with Bond™ primary antibody diluent (cat# AR9352, Leica) was done for all tissue samples. .. Immunofluorescent (IF) IHC staining was performed on two representative GBM tissue samples from the original cohort of patients used for DAB IHC staining, using identical primary antibodies and concentrations.

    Article Title: NADPH oxidase 1 mediates caerulein-induced pancreatic fibrosis in chronic pancreatitis
    Article Snippet: .. IHC for α-SMA, NF-ĸB and p-AKT in whole pancreas: Cells positive for these proteins were determined using 3,3-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (color: brown). .. Briefly, slides were incubated first with blocking buffer (3% BSA in PBS with 0.05% Tween-20) for 1 h at room temperature and then with antibody against α-SMA, NF-ĸB or p-AKT overnight at 4 °C.

    Expressing:

    Article Title: Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila
    Article Snippet: .. HRP expression was revealed overnight using the DAB substrate kit (Thermofisher). ..

    Incubation:

    Article Title: The Effects of Maekmoondong-Tang on Cockroach Extract-Induced Allergic Asthma
    Article Snippet: Subsequently, the sectioned tissues were incubated at 4°C overnight with an anti-MLC2 rabbit polyclonal antibody (1 : 50 dilution; Santa Cruz Biotechnology, CA, USA). .. After the slides were incubated with avidin-biotin peroxidase complex (ABC kit, Vestor Laboratories, CA, USA), the color was developed with 3,3′-diaminobenzidine tetrachloride (DAB; Zymed Laboratories, CA, USA). .. After immunohistochemical staining, the slides were counterstained with Harris's hematoxylin for 1 minute and then mounted with Canada balsam (Show Chemical Co. Ltd., Tokyo, Japan).

    Avidin-Biotin Assay:

    Article Title: The Effects of Maekmoondong-Tang on Cockroach Extract-Induced Allergic Asthma
    Article Snippet: Subsequently, the sectioned tissues were incubated at 4°C overnight with an anti-MLC2 rabbit polyclonal antibody (1 : 50 dilution; Santa Cruz Biotechnology, CA, USA). .. After the slides were incubated with avidin-biotin peroxidase complex (ABC kit, Vestor Laboratories, CA, USA), the color was developed with 3,3′-diaminobenzidine tetrachloride (DAB; Zymed Laboratories, CA, USA). .. After immunohistochemical staining, the slides were counterstained with Harris's hematoxylin for 1 minute and then mounted with Canada balsam (Show Chemical Co. Ltd., Tokyo, Japan).

    Immunostaining:

    Article Title: A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins
    Article Snippet: Cells were incubated with anti-myc 9E-10 antibody and goat anti-mouse secondary antibody (1:100) (HyClone). .. For immunostaining, cells at about 100% confluency were fixed with 2% paraformaldehyde-0.2% glutaraldehyde and stained with anti-myc (1:1,000) followed by anti-mouse secondary antibody and diaminobenzidine substrate (Lifetech). .. For FACS of transiently expressed CB5, A7 monolayers were transfected with pB5myc or vector only.

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    Thermo Fisher 3 3 diaminobenzidine dab
    Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) <t>3,3′</t> <t>diaminobenzidine</t> <t>(DAB),</t> used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.
    3 3 Diaminobenzidine Dab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab - by Bioz Stars, 2021-03
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    Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) 3,3′ diaminobenzidine (DAB), used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.

    Journal: The European Journal of Neuroscience

    Article Title: Central nervous system neurons acquire mast cell products via transgranulation

    doi: 10.1111/j.1460-9568.2005.04429.x

    Figure Lengend Snippet: Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) 3,3′ diaminobenzidine (DAB), used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.

    Article Snippet: The first made use of conventional staining with 3,3′ diaminobenzidine (DAB) (Immunopure DAB, Pierce) and the second, a silver intensification followed by gold toning of the DAB product (not shown).

    Techniques: Derivative Assay, Binding Assay

    Cell surface expression of B5 in porcine cells. (A) Cartoon of the predicted structure of B5 as an integral membrane protein with a predicted C-terminal α-helix. The C terminus and N terminus are labeled. (B) Cells transiently transfected with pB5-myc or vector only (pcDNA-myc) fixed at 48 h posttransfection and stained with anti-myc antibody, a secondary, peroxidase-conjugated antibody and diaminobenzidine substrate. (C) FACS of SK6-A7 cells transiently transfected with pcDNA vector (left) or pB5-myc (right) and stained with anti-myc (9E10) and a secondary, FITC-conjugated antibody (gray lines). Black peaks are cells in the absence of primary anti-myc antibody. (D) FACS of the indicated stable porcine cell line exposed to anti-Xpress and a secondary, FITC-conjugated antibody. Cells were permeabilized (P) with methanol-acetone or not permeabilized (unpermeabilized [U]) before staining. Neo is a vector-only-transformed cell line. NB5 is a stable cell line made with an N-terminal Xpress epitope-tagged B5. HB1-9 is a stable untagged HVEM-expressing porcine cell line. (E and F) NB5 and HB1-9 cells surface labeled with biotin and lysed as described in Materials and Methods. Lysates normalized for protein concentration were immunoprecipitated with preimmune serum (Pre) or with anti-HVEM polyclonal serum (HVEM), or anti-Xpress monoclonal antibody (XPRS). Proteins visualized in Western blots hybridized with streptavidin-conjugated peroxidase and enhanced chemiluminescence substrate (Amersham) (E) or anti-Xpress monoclonal antibody and alkaline phosphatase-conjugated anti-mouse secondary antibody and NBT/BCIP (F). The positions of molecular mass markers (in kilodaltons) are shown to the left of the gels. The arrow points to B5 at about 43 kDa.

    Journal: Journal of Virology

    Article Title: A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins

    doi: 10.1128/JVI.79.12.7419-7430.2005

    Figure Lengend Snippet: Cell surface expression of B5 in porcine cells. (A) Cartoon of the predicted structure of B5 as an integral membrane protein with a predicted C-terminal α-helix. The C terminus and N terminus are labeled. (B) Cells transiently transfected with pB5-myc or vector only (pcDNA-myc) fixed at 48 h posttransfection and stained with anti-myc antibody, a secondary, peroxidase-conjugated antibody and diaminobenzidine substrate. (C) FACS of SK6-A7 cells transiently transfected with pcDNA vector (left) or pB5-myc (right) and stained with anti-myc (9E10) and a secondary, FITC-conjugated antibody (gray lines). Black peaks are cells in the absence of primary anti-myc antibody. (D) FACS of the indicated stable porcine cell line exposed to anti-Xpress and a secondary, FITC-conjugated antibody. Cells were permeabilized (P) with methanol-acetone or not permeabilized (unpermeabilized [U]) before staining. Neo is a vector-only-transformed cell line. NB5 is a stable cell line made with an N-terminal Xpress epitope-tagged B5. HB1-9 is a stable untagged HVEM-expressing porcine cell line. (E and F) NB5 and HB1-9 cells surface labeled with biotin and lysed as described in Materials and Methods. Lysates normalized for protein concentration were immunoprecipitated with preimmune serum (Pre) or with anti-HVEM polyclonal serum (HVEM), or anti-Xpress monoclonal antibody (XPRS). Proteins visualized in Western blots hybridized with streptavidin-conjugated peroxidase and enhanced chemiluminescence substrate (Amersham) (E) or anti-Xpress monoclonal antibody and alkaline phosphatase-conjugated anti-mouse secondary antibody and NBT/BCIP (F). The positions of molecular mass markers (in kilodaltons) are shown to the left of the gels. The arrow points to B5 at about 43 kDa.

    Article Snippet: For immunostaining, cells at about 100% confluency were fixed with 2% paraformaldehyde-0.2% glutaraldehyde and stained with anti-myc (1:1,000) followed by anti-mouse secondary antibody and diaminobenzidine substrate (Lifetech).

    Techniques: Expressing, Labeling, Transfection, Plasmid Preparation, Staining, FACS, Transformation Assay, Stable Transfection, Protein Concentration, Immunoprecipitation, Western Blot

    Effects of MMDT treatment on airway remodeling in lung tissue (MLC2 immunohistochemistry). (a) Stainedmouse lung sections and (b) MLC2 area ( μ m 2 ). Mouse lung sections were stained for MLC2 detection. The sections were incubated at 4°C overnight with an anti-MLC2 goat polyclonal antibody (1 : 50). The slides were then incubated with avidin-biotin peroxidase complex, and color was developed using 3,3-diaminobenzidine tetrachloride. The arrows indicate MCL2 positive cells (magnification 200x). Normal control mice treated with PBS only (NC), CKA-challenged mice treated with PBS (CKA), CKA-challenged mice treated with 10 mg/kg of Montelukast (MK), CKA-challenged mice treated with 100 mg/kg of MMDT (MMDT 100 mg/kg), CKA-challenged mice treated with 200 mg/kg of MMDT (MMDT 200 mg/kg), and CKA-challenged mice treated with 400 mg/kg of MMDT (MMDT 400 mg/kg). The data are shown as the mean ± S.E.M. Statistical analysis was conducted by one-way ANOVA followed by the Newman-Keuls Multiple Comparison test (significantly different from NC, ** P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Effects of Maekmoondong-Tang on Cockroach Extract-Induced Allergic Asthma

    doi: 10.1155/2014/958965

    Figure Lengend Snippet: Effects of MMDT treatment on airway remodeling in lung tissue (MLC2 immunohistochemistry). (a) Stainedmouse lung sections and (b) MLC2 area ( μ m 2 ). Mouse lung sections were stained for MLC2 detection. The sections were incubated at 4°C overnight with an anti-MLC2 goat polyclonal antibody (1 : 50). The slides were then incubated with avidin-biotin peroxidase complex, and color was developed using 3,3-diaminobenzidine tetrachloride. The arrows indicate MCL2 positive cells (magnification 200x). Normal control mice treated with PBS only (NC), CKA-challenged mice treated with PBS (CKA), CKA-challenged mice treated with 10 mg/kg of Montelukast (MK), CKA-challenged mice treated with 100 mg/kg of MMDT (MMDT 100 mg/kg), CKA-challenged mice treated with 200 mg/kg of MMDT (MMDT 200 mg/kg), and CKA-challenged mice treated with 400 mg/kg of MMDT (MMDT 400 mg/kg). The data are shown as the mean ± S.E.M. Statistical analysis was conducted by one-way ANOVA followed by the Newman-Keuls Multiple Comparison test (significantly different from NC, ** P

    Article Snippet: After the slides were incubated with avidin-biotin peroxidase complex (ABC kit, Vestor Laboratories, CA, USA), the color was developed with 3,3′-diaminobenzidine tetrachloride (DAB; Zymed Laboratories, CA, USA).

    Techniques: Immunohistochemistry, Staining, Incubation, Avidin-Biotin Assay, Mouse Assay

    Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.

    Journal: Frontiers in Surgery

    Article Title: Glioblastoma Multiforme Cancer Stem Cells Express Components of the Renin–Angiotensin System

    doi: 10.3389/fsurg.2016.00051

    Figure Lengend Snippet: Representative 3,3-diaminobenzidine immunohistochemical stained images demonstrating cytoplasmic expression of SOX2 [(A), brown], PRR [(B), brown] by cells within GBM, and the endothelium of the microvessels . ACE [ (C) , brown] was present only in the endothelium of the microvessels with no staining of the cells within the tumor. Cytoplasmic and nuclear staining of ATIIR1 [ (D) , brown] and ATIIR2 [ (E) , brown] was observed on the cells within the tumor and the endothelium of the microvessels. Cell nuclei were counterstained with hematoxylin [ (A–E) , blue]. Original magnification: 400×.

    Article Snippet: 3,3-Diaminobenzidine (DAB) IHC staining for SOX2 (1:500; cat# PA094, Thermo Fisher, Scientific, Scoresby, VIC, Australia), PRR (1:2000; cat# ab40790, Abcam, Cambridge, UK), ATIIR1 (1:30; cat# ab9391, Abcam), ATIIR2 (1:2000; cat# NBP1-77368, Novus Biologicals, LLC, Littleton, CO, USA), ACE (1:100; cat# MCA2054, AbD Serotec, Kidlington, UK) diluted with Bond™ primary antibody diluent (cat# AR9352, Leica) was done for all tissue samples.

    Techniques: Immunohistochemistry, Staining, Expressing